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Quantitation Database
MSD Productivity ChemStation Getting Started
What is a quantitation database?
The quantitation database lists the significant details about
each compound you are looking for.
What kind of data is required in the quantitation database?
For each compound you want to quantify, the quantitation
database should include:
• One entry that identifies the compound you are looking for,
including details such as:
- Retention time
- Quantitation parameters
- Identification selection criteria
- Method for calculating qualifier ion ratios
- Acceptable range for the relative response
- Mathematical treatment applied to calibration data for a
compound
- Data points used in the calibration curve
• One entry that identifies the target ion (usually the base peak
ion) in the compound you are looking for
• Two or more entries for ions that further qualify the
presence of the compound. (For example, these ions will
always appear with the compound's peak ion and always in
the same ratios to it.)
• Any internal standards you will be using
This sounds difficult to do, but AutoQuant Setup can identify
these ions for you automatically. See
set up a quantitation database?
this works.
How big is the quatitation database?
To quantify a single compound, the quantitation database could
consist of as little as three entries:
• The compound's base peak ion
Understanding Quantitation
How do I use AutoQuant to
on page 63 for details on how
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