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Understanding Quantitation
Tutorial – Using AutoQuant Setup
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This tutorial will guide you through the steps of using
AutoQuant Setup to create a quantitation database. This
exercise is intended to illustrate how quickly you can create and
use a quantitation database using AutoQuant Setup. It should
take you about 5 minutes to complete.
During this exercise you will create a method containing a
quantitation database that can identify and quantify Biphenyl,
Chlorobiphenyl, and Methyl palmitate.
To do this you will:
1 Load the default method DEFAULT.M supplied with your
ChemStation.
2 Load the demo data file EVALDEMO.D supplied with your
ChemStation.
3 Use the demo spectrum library DEMO.L supplied with your
ChemStation.
4 Use AutoQuant Setup to create a quantitation database with
the following compounds:
- Dodecane
- Biphenyl
- Chlorobiphenyl
- Methyl palmitate
The resulting method and quantitation database will be able to
identify and quantify Biphenyl, Chlorobiphenyl, and
Methyl palmitate.
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