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Related Manuals for Multi Channel Systems MEA2100-Mini-System
Summary of Contents for Multi Channel Systems MEA2100-Mini-System
- Page 1 MEA2100-Mini-System Manual...
- Page 2 Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher...
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Page 3: Table Of Contents
Table of Content Welcome to the MEA2100-Mini-System ..............5 Before You Start......................6 Important Safety Advice ....................6 Guarantee and Liability ....................7 Hardware Components ....................8 Headstage MEA2100-Mini ..................8 Signal Collector Unit MCS-SCU ................... 9 Interface Board MCS-IFB 3.0 Multiboot ............... 9 Front Panel ...................... - Page 4 Appendix…………………………………………………………………………………….26 Technical Specifications ..................... 27 Pin Layout ........................31 MCS-IFB 3.0 Multiboot Interface Board ..............31 MEA2100-Mini Interface Board: Digital IN / OUT Connector ........31 MCS-IFB 3.0 Multiboot Interface Board: 10-Pin Connector for Analog IN ....32 ME2100 Interface Board: 20-Pin Connector (JTAG) for Digital Signal Processor ..33 Contact Information ....................
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Page 5: Welcome To The Mea2100-Mini-System
Welcome to the MEA2100-Mini-System The MEA2100-Mini-System is a miniaturized in vitro recording system. The state of the art power saving electronics allow to benefit from almost all advantages of the MEA2100 platform and still be able to operate the system continuously in the incubator. Headstages have 60 recording channels and two integrated stimulators each. -
Page 6: Before You Start
Before You Start Important Safety Advice Warning: Make sure to read the following advice prior to installation or use of the device and the software. If you do not fulfill all requirements stated below, this may lead to malfunctions or breakage of connected hardware, or even fatal injuries. Warning: Always obey the rules of local regulations and laws. -
Page 7: Guarantee And Liability
The operator will receive these no later than on conclusion of the contract. Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software. It is up to the user to use good laboratory practice to establish the validity of his / her findings. -
Page 8: Hardware Components
Hardware Components Headstage MEA2100-Mini The first element of the MEA2100-Mini-System is the headstage with small footprint and operable in a humid incubator. The MEA2100-Mini headstage is available with 60 channels and can be with a cable length of 5 m. -
Page 9: Signal Collector Unit Mcs-Scu
Signal Collector Unit MCS-SCU The second element of the MEA2100-Mini-System is the signal collector unit MCS-SCU for up to four headstages. The MCS-SCU also contains a connector to control up to four independent optical stimulation devices. The control functions are integrated into the Multi Channel Experimenter software. -
Page 10: Front Panel
The power LED near to the MCS logo on the front panel of the interface board should light up when the MEA2100-Mini-System is "ON" and the device is connected to the power line. If not, please check the power source and cabling. - Page 11 Power IN Connect the power supply unit here. This power supply powers both, the headstage and the interface board of the MEA2100-Mini-System. The device needs 12 V and 2.5 A / 30 W. Ground If an additional ground connection is needed, you can connect this plug with an external ground using a standard common jack (4 mm).
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Page 12: Side Panel
Analog Channels 1 and 2 Two of the analog channels described above (analog channel 1 and 2) are directly accessible via two Lemo connectors. JTAG Connector The JTAG connector has access to the digital signal processor DSP, but the DSP is not yet in use in MEA2100-Mini-Systems. -
Page 13: Software Multi Channel Suite
Please contact your local retailer for more information on recommended computer hardware. Important: You need to have installed the latest MEA2100-Mini-System driver to operate the MEA2100-Mini-System, which is automatically installed with the data acquisition software. -
Page 14: Multi Channel Experimenter
The icon indicates which MCS-SCU it represents. 1 and 2 indicate the signal collector unit connected to port 1 or 2 of the interface board. Once the MEA2100-Mini-System is added to the instrument configuration, the MEA2100-Mini stimulator and the MCS-SCU stimulator will also become available as instruments. - Page 15 Drag the MEA2100-Mini data source icon into the main window and double click it to open the control window. The MEA2100-Mini data source control window shows all available data channels for all connected headstages, and optionally also the additional analog input channels from the interface board. One headstage is visible at a time.
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Page 16: System Setup
System Setup Connecting the Hardware Components The setup of the MEA2100-Mini-System is very simple. Familiarize yourself with the components as described in the chapters above, then go through the step-by-step setup instruction below. Provide a power supply in the immediate vicinity of the installation site. -
Page 17: Setting Up The Mea
Check the power LED. It should light up as soon as the power line is connected, and the toggle switch is switched on. If not, check the power source and cabling. Warning: It is recommended to switch off the MEA2100-Mini-System on the interface board whenever connecting or disconnecting headstages. -
Page 18: First Functional Tests
Test Model Probe Please use the provided test model probe to test the functionality of the MEA2100-Mini-System. Connect the test model probe "Test-60MEA" to each headstage. The test model probe simulates a MEA with a resistor of 220 k and a 1 nF capacitor between ground and electrode for each of the 60 electrodes. - Page 19 Generator" manual and datasheet "60MEA2100-SG". Temperature Controller TC01 or TC02 The MEA2100-Mini-System features a heating element with a PT100 temperature sensor. If you connect a temperature controller TC02 for example, to the heating element via D-Sub 9 connector, the heating element guarantees constant temperature conditions for the biological sample, placed on the MEA.
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Page 20: Mcs Filter Configuration
MCS Filter Configuration The MCS Filter Configuration software is an add on program for changing the hardware filter settings by the software. No firmware update is necessary. You can operate the MCS Filter Config software with MEA2100-Systems, MEA2100-Mini-Systems and ME2100-Systems. Please install the MCS Filter Configuration software by clicking the “McsFilterConfig.exe”... - Page 21 Select the cut off frequency of the filter from the second drop-down box. The message “The cut off frequency of the filter. The amplitude is reduced by 3 db at this frequency. A filter with Bessel characteristic is used.” is shown. If displayed, the option “Custom”...
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Page 22: Service And Maintenance
Service and Maintenance Cleaning the Connector Warning: Please be very careful when handling the headstage or cleaning the device! The pins can be damaged easily. You should clean the contact pins of the MEA2100-Mini headstage carefully with a soft tissue and alcohol or isopropanol from time to time, especially if you have problems with the noise level. -
Page 23: About Troubleshooting
Multi Channel Systems has put all effort into making the product fully stable and reliable, but like all high-performance products, it has to be handled with care. -
Page 24: Triggering / Digital Input Does Not Work
The MEA2100-Mini-System can only accept TTL signals as a digital input stream. The TTL pulse needs to be optimized according to the sampling rate. Otherwise, a detection of the trigger cannot be guaranteed. -
Page 25: Mea Is Defective
MEA is defective MEAs wear out after multiple uses or over a longer time of use, for example, for long-term cultures. This is considered a normal behavior. MEAs are also easily damaged by mishandling, for example, if wrong cleaning solutions or too severe cleaning methods are used or if the recording area is touched. -
Page 26: Missing Spikes Or Strange Signal Behavior
Missing Spikes or Strange Signal Behavior MEAs wear out after multiple uses or over a longer time of use, for example for long-term cultures. The insulation layer gets thin over time. This is considered a normal behavior. Possible causes: ? The insulation layer is too thin. As a result, the MEA gets the behavior of a low pass filter. This means, that the signal frequency may be shifted to a lower frequency, and spikes are missing. -
Page 27: Appendix
Appendix Technical Specifications MEA2100-Mini-System Warning: The device may only be used together with microelectrode arrays (MEAs) from Multi Channel Systems MCS GmbH and only for the specified purpose. Damage of the device and even fatal injuries can result from improper use. Do not open the headstage, the signal collector unit or the interface board and do not change hardware configuration as it could lead to improper behavior of the system. - Page 28 Integrated Stimulus Generators Output current ± 1.5 mA Current output compliance voltage ± 16 V Output voltage ± 10 V Voltage output compliance current ± 20 mA Stimulation pattern Almost arbitrary patterns Number of stimulation channels 2 independent stimulation patterns per 60 channels Resolution 16 bit Integrated Heating Element...
- Page 29 MCS-IFB 3.0 Multiboot Interface Board Dimensions (W x D x H) 250 mm x 83 mm x 25 mm Weight 300 g Front Panel 4 Digital inputs Lemo connector, EPL.00.250 NTN 4 Digital outputs Lemo connector, EPL.00.250 NTN 2 Auxiliary channels (not in use) Lemo connector, EPL.00.250 NTN Rear panel 1 16 bit Digital In / Out...
- Page 30 Power supply unit (MPU 30) Input voltage 90 – 264 VAC @ 47 – 63 Hz Output voltage 11 – 13 V Max. Power 30 W Mark of conformity CE, TÜV, cUL European standards EN60601 Software Operating system Microsoft Windows ® 10, 8.1, 32 or 64 bit, English and German version supported Multi Channel Experimenter Version 2.6.0 and higher...
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Page 31: Pin Layout
Pin Layout MCS-IFB 3.0 Multiboot Interface Board MEA2100-Mini Interface Board: Digital IN / OUT Connector 68-Pin MCS Standard Connector 3 - 10 Digital output channels bit 0 - 7 11 - 14 15 - 22 Digital output channels bit 8 - 15 23 - 26 27 - 34 Digital input channels bit 0 - 7... -
Page 32: Mcs-Ifb 3.0 Multiboot Interface Board: 10-Pin Connector For Analog In
Digital IN / OUT, separate available via Lemo Connectors Separate Digital IN Lemo Connectors Digital In 1 Bit 0 of the 16 bit digital input channels (Pin 27) Digital IN 2 Bit 1 of the 16 bit digital input channels (Pin 28) Digital IN 3 Bit 2 of the 16 bit digital input channels (Pin 29) Digital IN 4... -
Page 33: Me2100 Interface Board: 20-Pin Connector (Jtag) For Digital Signal Processor
MEA2100-Mini Interface Board: Lemo Connectors for Analog IN Two of the eight analog channels are additionally separate available via Lemo Connectors Analog IN 1, Channel 1, Pin 3 Analog IN 2, Channel 2, Pin 4 The gain factor for amplification of all analog channels is 2. ME2100 Interface Board: 20-Pin Connector (JTAG) for Digital Signal Processor Number Assign-... -
Page 34: Contact Information
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