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Setup
Cable Connections
Bath clamp headstage: After positioning the probe as described above, connect it to the
BATH PROBE socket (BATH ELECTRODES section).
Voltage electrode headstage: The high impedance probe for recording membrane
potential should be mounted on a micro-manipulator and connected to the VOLTAGE
PROBE socket (VOLTAGE ELECTRODE section).
High voltage current electrode: The holder should be mounted on a micro-manipulator
and the cable connected to the I ELECTRODE socket (CURRENT ELECTRODE section).
Command potential: If a computer or external generator is used for controlling the
clamp command potential, its signal should be connected to the front panel COMMAND
IN ÷10 input (COMMANDS section).
External monitoring: To monitor the microelectrode's potentials on an oscilloscope,
computer or a chart recorder, the following connections should be made:
V
: Membrane potential may be recorded from the Vm x10 connector (VOLTAGE
m
ELECTRODE section).
V
: The voltage of the current electrode can be monitored from the Ve connector on the
e
REAR PANEL.
The output will be the same as that reported on the CURRENT ELECTRODE METER,
showing the voltage across the current electrode when the CLAMP SELECTOR switch is
in the off position. (Recall that the meter indicates the current (Im) when the CLAMP
SELECTOR switch is in either the slow or fast position).
I
: The current signal is available from the I MONITOR and I MONITOR FILTERED outputs
m
(BATH ELECTRODES section).
Gain telegraph: Automatic monitoring of the Im gain can be achieved by connecting the
rear panel GAINE TELE BNC to the appropriate input on your analog-to-digital converter.
Power: The power cord should be connected to a properly grounded AC receptacle with
the line voltage specified on the instrument nameplate (REAR PANEL).
24
Publication 5720-001 REV1.0
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