Single Oocyte Setup With Direct Ground - Warner OC-725C User Manual

Setup

Single Oocyte Setup with Direct Ground

Applications where use of the bath clamp is not suitable (such as those with a very
long solution path to ground) can be configured using the alternate method of directly
grounding the bath as shown in Figure B. In this configuration, current is read from the
"high side" of the current output leg.
This method also requires the use of the optional DIFFERENTIAL VOLTAGE HEADSTAGE.
Two disadvantages are: (1) The noise levels of the current signal measured in the "high
side" is approximately double that obtained with a bath clamp, and (2) high levels
of clamp current could produce a substantial voltage drop across the solution series
resistance.
NOTES: The oocyte clamp has no provisions for compensating the voltage drop
described above. The differential voltage electrode should be placed close to
the oocyte and in the current path between the Vm electrode and ground.
Dual Ooctye Setup
Dual oocyte setup is accomplished using two clamps as illustrated in Figure C. Both
clamps must be configured to read current from the "high side" as described in the
section titled HIGH SIDE CURRENT MEASURING (see page 26) and each clamp must be
equipped with the optional DIFFERENTIAL VOLTAGE HEADSTAGE.
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