Bath Electrodes - Warner OC-725C User Manual

Oocyte clamp amplifier
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Control Description
In general, negative capacity compensation is not useful for oocyte clamp applications
since clamp speed is a function of (1) the current electrode resistance, (2) the RC time
constant of the oocyte (typically 1 MΩ in parallel with 0.5 µF), and (3) the compliance
voltage of the clamp current.

Bath Electrodes

The bath electrodes control block contains the bath probe connector, the current
meter, the gain select switches, and the i monitor output BNC's.
The current meter reads the voltage (V
) of the current electrode when the clamp
e
mode selector switch is off (see clamp section). A lit LED indicates voltage readings in
mV.
When in clamp mode (clamp mode
selector switch set to slow or fast), the
current meter displays the current (I
)
m
sensed by the bath electrode. A lit LED
indicates current readings in µA.
Instrument gain is set by the two gain
select controls. Gain is selected by a
7-position gain selector switch, which
ranges from 0.1 to 10 in 1-2-5 steps, and
a 3-position toggle switch which selects
the gain multiplier (x0.1, x1.0 and
x10). LED's indicate the gain multiplier
selection. The combination of these controls allows gain to be set from 0.01 to 100.
10
Publication 5720-001 REV1.0

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