Fig; F Ig. 4-5 Centering The Annular Phase Diaphragm (Light-Colored, In The Condenser) And The Phase Ring (Dark-Colored, In The Object) - Zeiss Axio Scope.A1 Operating Manual

Axio Scope.A1
• If the overlap is not exact (
lighter annular diaphragm must be recentered
with the aid of two hexagon screwdrivers
SW 1.5 ( F ig. 4-4/1 and 4). Adjust the two
6 3 3 H 6 3 3 H
centering screws ( F ig. 4-4/2 and 3) so that you
6 3 4 H 6 3 4 H
achieve a full overlap with the darker phase ring
( F ig. 4-5/B).
6 3 5 H 6 3 5 H
• Remove the auxiliary microscope from the tube
and replace it with the eyepiece.
In order to increase the image contrast, an
interference broadband filter, green 32 x 4, may be
mounted on the field diaphragm or inserted into
the color glass carrier (if available).
A complete phase contrast can only be achieved
when the light-colored annular diaphragm (in the
condenser) overlaps exactly with the dark-colored
phase ring (in the object) in the illumination beam
path ( F ig. 4-5/B).
6 3 6 H 6 3 6 H
M60-2-0007 e 05/08
OPERATION
Illumination and Contrasting Method
F ig. 4-5/A), the
6 3 2 H 6 3 2 H

Fig. 4-5

Centering the annular phase
diaphragm (light-colored, in the
condenser) and the phase ring
(dark-colored, in the object)
Carl Zeiss
75