Regions; Gating; Data Storage - Beckman Coulter Navios Instructions For Use Manual

Operation Principles
Plot Display

Regions

To analyze data or gate plots, you must first create and assign regions to these tasks. You can create
five different types of regions (see
• Linear
• Rectangular
• Quadrant/FlexQuad
• Polygonal
• Elliptical
• Contour
Once a region is created it can be assigned to function in a specific way. The functions that are
available include:
• Analysis
• Prime
• CAL (calibration)

• Gating

• Linked
• Listmode gating (LIVEGATE)
• Contour AutoGate
• Elliptical AutoGate
• Positives analysis
• Minimum Count.
These functions are not available for all region types.
Gating
The software lets you use gating to specify that only certain cells are to be analyzed. A gate can be
defined as the events that are inside or out of one or more regions.

Data Storage

Sample results can be printed out, saved to removable media, saved to a local hard drive or saved to
a network drive. You can store sample results in the form of a list of the measurements from each
cell, called listmode data. Listmode data can be replayed into plots or archived for analysis later.
Histograms can also be saved to a file. The runtime protocol is always saved to the listmode file.
3-14
Regions Introduction). The region types are:
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