Log-Lin; Automatic Statistic Percentiles; Absolute Count Calibration; Setting A Cal Factor For Absolute Counts - Beckman Coulter Navios Instructions For Use Manual

Log-Lin

Selecting the Log-Lin mode calculates the mean values by determining the arithmetic mean of the
raw ADC channels and then converting this to its relative linear channel equivalent.

Automatic Statistic Percentiles

This is the channel, which divides the events in a region by the percentile value. The default
percentile is 50 (the median) but can be set to request any desired value. Different values may be set
for both the X and Y axis.

Absolute Count Calibration

Use absolute count calibration particles for the calculation of absolute numbers of cells present in
a sample.
As a sample is analyzed, the numbers of a particular cell type in a region, and the numbers of
absolute count calibration particles present in a CAL region are compared, and the absolute
concentration of the cell type is then calculated automatically.
A listmode files replays with its runtime Cal Factor. Use this screen to enter a different Cal Factor to
calculate individual listmode absolute counts.
NOTE
•
A CAL region MUST be set for the system to be able to calculate an absolute count.
See
•
Listmode replay uses the runtime protocol Cal Factor unless you select a different Cal Factor before
replaying the file.
To display the absolute count,

Setting a CAL Factor for Absolute Counts

From the list of
select the Lot Number currently in use.
Manual Absolute Count Calibration Value
Enter a
required Lot is not present in the list. A manual
calibration value is only used for the current session, it
cannot be saved. A listmode file replays with its
runtime Cal Factor.
NOTE
The number of events in the CAL region must be
greater than 1000, otherwise absolute counts are not
determined.
773232AH
Setting a CAL Region.
Cells/μL
Available Absolute Count Calibrators
from the Analysis/Select Results
,
if the
Using Navios Software 10
Analysis Menu
menu option.
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