Idexx VetTest Operator's Manual
  1. Manuals
  2. Brands
  3. Idexx Manuals
  4. Measuring Instruments
  5. VetTest
  6. Operator's manual
Idexx VetTest Operator's Manual

Idexx VetTest Operator's Manual

Chemistry
Hide thumbs
Table of Contents

Advertisement

Quick Links

IDEXX VetTest*
Chemistry Analyzer
Operator's Manual

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the VetTest and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Idexx VetTest

Summary of Contents for Idexx VetTest

  • Page 1 IDEXX VetTest* Chemistry Analyzer Operator’s Manual...
  • Page 3 IDEXX Laboratories. *VetTest, VetTrol, Cornerstone, and SNAP are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the United States and other countries. All other product and company names and logos are trademarks of their respective holders.

  • Page 5: Table Of Contents

    Safety Symbols on the Product....................xiv Connection to a Power Source ....................xiv Personal Safety ........................xiv Important Things to Know About the VetTest* Analyzer ........A–1 Overview ............................A–1 Slide Packaging and Storage ....................... A–1 Profile Interpretation (Canine and Feline only) ................A–1 Star Reference System ........................
  • Page 6 IDEXX UPRO Control ........................F–3 Preparing the VetTrol Quality Control Fluid ..................F–3 Preparing the UPRO Control ......................F–3 Performing the Quality Control Procedure on the VetTest Analyzer ..........F– Documenting Quality Control Results ...................F– Performing the Ca Offset Procedure .....................F– Other Maintenance ..................... G–1 General Upkeep and Cleaning .....................G–1...
  • Page 7 UV Lamp Replacement ........................G–2 Autocalibration ..........................G–2 Autocalibration Kit .........................G–2 Chemistry Description and Guide ...............H–1 Introduction to Biochemical Profiling .................... H–1 Enzymes ............................H–1 Enzymes as Indicators of Organ Damage ................H–1 ........................H–2 Albumin/ALB ..........................H–3 Alkaline phosphatase/ALKP ......................H– Alanine aminotransferase/ALT (SGPT) ..................
  • Page 8 Appendix: Reference Ranges ................L–1 Canine—Puppies .........................L–1 Canine—Adults ..........................L–2 Canine—Geriatric .........................L–3 Feline—Kittens ..........................L– Feline—Adults ..........................L– Feline—Geriatric ..........................L– Equine—Yearlings .........................L– Equine—Foals ..........................L–8 Equine—Adults ..........................L– Equine—Mares at Stud .......................L–10 Dairy Cows ...........................L–11 Beef Cattle ...........................L–12 Llama ............................L–13 Sea Turtles ...........................L–1 Provisional Reference Ranges ....................L–1 Avian—Parrots ........................L–1 Avian—Cockatoos .......................L–1 Avian—Budgerigar .......................L–1...

  • Page 9: Preface

    Preface About this Guide Use This Section... To Learn About... A Important Things to Know About How the analyzer works the VetTest* Analyzer Slide packaging and storage Profile interpretation Star reference system Software updates Available chemistries B System Overview and Installation...
  • Page 10 IDEXX VetTest* Chemistry Analyzer Operator’s Manual Use This Section... To Learn About... K Appendix: Differences in Results Differences in results from the VetTest analyzer as compared to other analyzers or your expectations L Appendix: Reference Ranges Reference ranges for a variety of species M Unit Conversions Conversion factors (U.S.

  • Page 11: Idexx Technical Support Contact Information

    Preface IDEXX Technical Support Contact Information United States/Canada/Latin America .............................. www.idexx.com Europe ............................Australia ..............................www.idexx.com.au Japan ..............................www.idexx.co.jp New Zealand .............. Asia ...........

  • Page 12: International Symbol Descriptions

    (such as use by, temperature limitations, batch code, etc.). IDEXX Laboratories has adopted the use of international symbols on our analyzers, product boxes, labels, inserts, and guides in an effort to provide our users If you are unsure of the meaning for a particular symbol, see the table below for a description of each symbol that can be found on IDEXX Laboratories packaging.
  • Page 13 Preface Symbol Description Symbol Description Authorized Representative in the WEEE Directive 2002/96/EC European Community Représentant agréé pour la C.E.E. Rappresentante autorizzato nella Comunitá Europea Representante autorizado en la Comunidad Europea Manufacturer Biological Risks Fabricant Risques biologiques Hersteller Biogefährlich Ditta produttrice Rischi biologici Fabricante Riesgos biológicos...

  • Page 14: General Safety Summary

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual General Safety Summary Please review the precautions on this page and on the analyzer to avoid personal injury, fire hazard, or damage to the product. Use of the equipment in a manner not specified by the manufacturer may impair the equipment’s safety features.

  • Page 15: A Important Things To Know About The Vettest* Analyzer

    Important Things to Know About the VetTest* Analyzer Overview The VetTest* Chemistry Analyzer is a blood and urine testing instrument that analyzes up to 12 biochemical tests simultaneously, using a single sample of either serum, plasma, or urine, in about six minutes.

  • Page 16: Star Reference System

    You can contribute to this important work each time you run a test by first enabling your VetTest analyzer, then indicating if this animal is sick or questionable, or healthy on the appropriate VetTest analyzer screen display. To do so, first enable the patient “Health Screen”...

  • Page 17: Vettest Chemistries

    Urea Glucose URIC Uric Acid Note: Globulin is calculated by subtracting albumin from total protein. Several prepackaged panels and profiles are also available for the VetTest analyzer. These include: Profile/Panel Name Included Chemistries General Health Profile ALB, ALKP , ALT, AMYL, Ca...

  • Page 18: Vettest Species

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual VetTest Species Adult Geriatric (> 8 years) Adult Geriatric (> 8 years) Equine Yearling Foal Adult Mare at Stud Bovine Beef Cattle Dairy Cow Avian Budgerigar Cockatoos (Grey Cheek, Moluccan, Umbrella) Cockatiel Canary Conure...

  • Page 19: B System Overview And Installation

    “temperature warning” message and results may show some drift. Make sure the VetTest* Chemistry Analyzer is not in direct sunlight. The analyzer is a Choose a flat, stable surface for the analyzer. Do not move it frequently. Be sure to leave adequate room for the pipettor and paper roll, and to load slides.

  • Page 20: Precautions

    Take care not to spill water or other fluids on the analyzer. Do not use any of the following liquids, abrasives, or aerosol sprays on or near the VetTest analyzer, as they may damage the outer case and may influence the analysis results: –...

  • Page 21: Vettest Analyzer Components (Front And Back Views

    Remove the VetTest analyzer from the shipping carton by firmly gripping the base of the machine and lifting it; place it on the work surface with the front facing forward. Keep the shipping carton in case you need it for future transportation.
  • Page 22 (See the “To install the transit clamps” section that follows.) Figure B-4: Top views of the VetTest analyzer with transit clamps in place and the transit clamps removed If you are using a SNAP* Reader with the VetTest analyzer, position it in place of the cover (removed in step 1).

  • Page 23: Connecting The Vettest Pipettor

    Connecting the VetTest Pipettor The VetTest pipettor is a sensitive electronic instrument that is connected to the VetTest analyzer by wire and plastic tubing. The pipettor draws and dispenses precise volumes of sample through its disposable plastic pipette tip. Always treat the pipettor with care.

  • Page 24: Inserting The Paper Roll

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Inserting the Paper Roll IDEXX recommends using only VetTest brand thermal paper for best results. The thermal paper is coated on one side; the print only shows on the coated side. The paper must be inserted correctly for print to appear.

  • Page 25: Connecting The Power Cord And Turning On The Analyzer

    “Settings” from the Main Menu. Scroll through each option, Time and Date The time and the date are set when the VetTest analyzer is installed. You can change the time and date whenever necessary. Follow the onscreen instructions to reach the “Settings"...

  • Page 26: Connecting Your Printer

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Printout Settings The VetTest analyzer has its own internal printer. You can also connect to an external printer (see Connecting Your Printer each printing option to adjust the printer, depending on your configuration. Paper Cutting —For use with the internal VetTest printer only. This option sets the number of blank lines that appear after the results print.

  • Page 27: Connecting An Idexx Snap* Reader Analyzer

    System Overview and Installation Note: The relationship of the keys on the external keyboard with the VetTest keypad are as follows: Keypad External Keyboard ENTER BACKSPACE Restrictions: use spaces or the following symbols when entering a patient identification: Period Colon...
  • Page 28 IDEXX VetTest* Chemistry Analyzer Operator’s Manual B–10...

  • Page 29: C Sample Collection And Preparation

    Sample Collection and Preparation The VetTest* Chemistry Analyzer is validated for animal plasma, serum or urine analysis only, and should not be used for the analysis of other body fluids. The VetTest analyzer is for veterinary use only. Plasma and Serum Collection and Preparation Minimize Stress—Make sure the pet is calm when taking the blood sample.

  • Page 30: Serum Sample Preparation

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Serum Sample Preparation To prepare a serum sample: Use the appropriate tube. Use the appropriate sample collection device. Gently draw the patient’s blood and transfer it, if necessary, to a serum tube. Notes: If using a syringe and needle draw, be sure to remove the needle before transferring the blood into the tube to avoid hemolysis.

  • Page 31: Centrifuged Sample Inspection

    A urine analysis on the VetTest analyzer can only be run with urine protein slides or urine creatinine slides. The IDEXX Urine P:C Ratio (urine protein:creatinine [UPC] ratio) is the first test to run urine on the VetTest analyzer. It consists of two slides specifically validated and calibrated for urine samples:...

  • Page 32: Urine Sample Volumes

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Urine Sample Volumes Note: The IDEXX Urine Protein:Creatinine Sample Preparation Kit prepares the sample for the urine creatinine test so the VetTest analyzer can calculate the most accurate and quantitative UPC ratio result. Urine Sample Preparation To prepare a urine sample: Obtain a urine sample through cystocentesis.

  • Page 33: Storing Urine Samples

    Chemistry testing on the VetTest requires serum or plasma. Note: A urine analysis on the VetTest analyzer can only be run with urine protein slides or urine creatinine slides and when performing UPC ratios.
  • Page 34 IDEXX VetTest* Chemistry Analyzer Operator’s Manual...

  • Page 35: D Basic System Operation

    Selected VetTest* chemistry slides. Slides can be used directly from the freezer or refrigerator. You do not need to bring them to room temperature before using them on the VetTest analyzer. Keep individual slides in their foil packs until just before inserting them into the analyzer.

  • Page 36: Enter Patient Identification (Id

    30 characters. If you do not want to include a client first and last name, press E on the VetTest keypad or the Enter key on the external keyboard. Note: Including a client first and last name enables practice information management systems to better determine with which patient and client a set of results should be associated.

  • Page 37: Preparing The Pipettor For A Sample

    Basic System Operation Preparing the Pipettor for a Sample When the VetTest analyzer is ready, it will prompt you to prepare the pipettor. To prepare the pipettor for a sample: Remove the pipettor from its holder on the VetTest analyzer.

  • Page 38: Postanalysis Steps

    A full slide drawer can jam the rotor. Also, if the drawer is empty at the start of slide insertion, any ejected unused slides will be easily identified and can be reloaded. Results Internal VetTest printer results Results from the internal VetTest printer are shown here: Software version Analyzer serial number 06824 Ver X.X...
  • Page 39 Basic System Operation External printer results External printer results are shown here: Bay View Animal Clinic 7264 Bradley Lane Nome, Alaska 34728 Species: Adult Canine Patient: Mitzy Ver: X.X Client: Bob Jones Date: 16-Jul-2007 02:14PM Test Results Reference Range Indicator NORMAL HIGH 3.19 g/dl...
  • Page 40 IDEXX VetTest* Chemistry Analyzer Operator’s Manual Profile Interpretation printout An example of the Profile Interpretation option results are shown here: Bay View Animal Clinic 7264 Bradley Lane Nome, Alaska 34728 Species: Adult Patient: Mitzy Ver: X.X Client: Bob Jones Date: 16-Jul-2007 02:14PM Results of this profile may be consistent with the following conditions...

  • Page 41: E Advanced Operations

    See the following chart for linearity ranges on individual chemistries. If a value is required, it will be necessary to dilute the sample and repeat the test. Note: A dilution message is printed on the internal VetTest* printer results. Analyzer Linearity Range Test U.S. Units S.I.

  • Page 42: Dilution Protocols

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Dilution Protocols The ability to perform dilutions on the VetTest analyzer allows you to qualify extremely elevated results. Plasma and Serum Dilutions The VetTest analyzer supports plasma and serum dilutions in two circumstances: When a test value is outside the linearity range of the VetTest analyzer When the sample contains interfering substances (e.g., medications) that cause a...

  • Page 43: Urine Dilutions

    Total Parts (Dilution Factor) Parts DI Water: the number entered into the VetTest when running the diluted sample Dilution Factor: the total number of parts in the diluted sample; the VetTest automatically multiplies the result by this number to correct for the dilution...

  • Page 44: Running A Dilution On The Vettest Analyzer

    7 – Dilutions, enter the number of diluent parts (parts saline or parts DI water) and press E. The species menu appears. Note: If you are running the most recent patient entered into the VetTest analyzer, select 2 – Current Sample. Then select 1 – Dilution, enter the number of diluent parts and press E.

  • Page 45: Patient Monitoring

    Enter the patient ID. Select the print option. Note: Results will print to the internal VetTest printer only. Important: When monitoring one patient (multiple samples) it is important to select “Monitor Patient” after each sample has been run. When patient monitoring is complete, you should print and file the results in the patient's permanent folder because...
  • Page 46 Previous results cannot be retrieved. Important! COMBINE RESULTS FROM A SINGLE SAMPLE ONLY. IDEXX does not recommend combining results from samples collected at different times, even from the same patient. Patient conditions can change even over short time...

  • Page 47: F Quality Control

    Quality Control The purpose of Quality Control (QC) is to verify that your VetTest* Chemistry Analyzer is functioning properly. The following QC procedure verifies both the VetTest optics groups and the integrity of your slides. If a QC test fails (i.e., test results fall outside of to determine the source of failure.

  • Page 48: Vettest Quality Control Panel

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual VetTest Quality Control Panel The VetTest Quality Control Panel includes all the chemistries needed to ensure a comprehensive quality control procedure on the VetTest analyzer. The Quality Control of quality control material. The six chemistries included are: calcium (Ca...

  • Page 49: Idexx Upro Control

    Be sure to bring a vial to room temperature before use. Immediately return Preparing the VetTrol Quality Control Fluid Use the VetTrol Quality Control fluid to test the VetTest analyzer’s optic lamps, the blood chemistry slides, and the UCRE slide.

  • Page 50: Performing The Quality Control Procedure On The Vettest Analyzer

    The current quality control lot numbers will be displayed. Select the number that corresponds with the number on your control vial (either VetTrol or UPRO Control). Proceed as if running a patient sample, following the prompts on the VetTest screen. Compare your results with the corresponding ranges on the printout.

  • Page 51: Documenting Quality Control Results

    The four calcium values will be printed at the end of the analysis on the internal printer. The values should all be in range for that QC lot. If one or all values are out of range, contact IDEXX Technical Support to determine if further steps need to be taken.
  • Page 52 IDEXX VetTest* Chemistry Analyzer Operator’s Manual...

  • Page 53: G Other Maintenance

    A VetTest dust cover should be used when the analyzer is turned off. Do not use the cover when the analyzer is turned on. The cover may cause the VetTest analyzer to overheat or cause the printer paper to jam.

  • Page 54: Pipettor Cleaning

    Autocalibration The autocalibration procedure allows the VetTest analyzer to compensate for lamp lamp, or, when instructed by IDEXX Technical Support during the life of the lamp to correct for minor changes. Autocalibration kits are provided by IDEXX free of charge. The materials provided in the kit are intended to be used together.
  • Page 55 (see Inserting the VetTest Software Disk more information). Insert the AUTOCAL 3SL Ver X.X diskette into the disk drive on your VetTest analyzer and turn on the power. Load the three slides as instructed on the VetTest analyzer screen. Use the chart below to guide in the proper identification of each slide to assure that the slides...
  • Page 56 IDEXX VetTest* Chemistry Analyzer Operator’s Manual...

  • Page 57: H Chemistry Description And Guide

    IDEXX VetTest* Chemistry Analyzer. Enzymes Eight tests for enzymes are available for use on the VetTest analyzer. Please read the following information as well as the section on the individual chemistry tests. The eight enzymes fall into one of two categories: those found in the cellular cytoplasm (or mitochondria) and those bound to cell membranes.
  • Page 58 IDEXX VetTest* Chemistry Analyzer Operator’s Manual In diseases such as viral hepatitis where the damage may be prolonged and progressive, the enzyme release will also be prolonged. Here, removal of enzyme from the circulation may not keep pace with enzyme release and the serum activity will remain high for as long as damage continues.

  • Page 59: Albumin/Alb

    Chemistry Description and Guide Albumin/ALB Albumin forms the largest fraction of the total serum protein in the healthy animal. It is synthesized solely in the liver, has a low molecular weight, and plays an important role in the transport of endogenous and exogenous compounds in bound form. Albumin also makes a major contribution to osmoregulation.

  • Page 60: Alkaline Phosphatase/Alkp

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Alkaline phosphatase/ALKP The enzyme alkaline phosphatase is found in many body tissues. Highest levels are found in the kidney cortex, small intestinal mucosa, and osteoblasts. In many instances the enzyme is present in the epithelial cells lining excretory ducts.

  • Page 61: Alanine Aminotransferase/Alt (Sgpt)

    Chemistry Description and Guide Alanine aminotransferase/ALT (SGPT) For practical purposes the enzyme alanine aminotransferase is specific to the liver in dogs and cats. It is found in the cellular cytoplasm and may be released into the blood during changes in cell membrane permeability or necrosis. Principal reason for performing the test: To investigate hepatic damage in dogs and cats.

  • Page 62: Amylase/Amyl

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Amylase/AMYL Important: Read this page in conjunction with the details on Lipase (LIPA). The enzymes amylase and lipase are found at high activity in the pancreas and to a lesser extent in the salivary glands, small intestinal mucosa, and liver. They have a short may be used in the confirmation of acute pancreatitis, but because of the slightly longer...

  • Page 63: Aspartate Aminotransferase/Ast (Sgot)

    Chemistry Description and Guide Aspartate aminotransferase/AST (SGOT) The enzyme aspartate aminotransferase is present in large amounts in the organs and tissues of dogs and cats. It is found in the cytoplasm and mitochondria of the cells and is released into the blood during changes in cell membrane permeability or necrosis. (See Enzymes The test is not a specific or sensitive indicator of damage to any organ or tissue.

  • Page 64: Calcium/Ca

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Calcium/Ca Calcium is an essential element which is involved in many body systems. These include the skeleton, enzyme activation, muscle metabolism, blood coagulation, and Factors governing the total plasma or serum concentration are complex and include interaction with other chemical moieties, proteins, and hormones.

  • Page 65: Cholesterol/Chol

    Chemistry Description and Guide Cholesterol/CHOL Serum cholesterol occurs at high concentration in the esterified form and at much lower concentration in the free form. Cholesterol is synthesized in the liver and other tissues and is also absorbed in the free form from the small intestine. It is esterified in the liver and is the precursor of steroid hormones.

  • Page 66: Creatine Kinase/Ck

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Creatine kinase/CK Creatine kinase is found at high activity only in the cytoplasm of cardiac and skeletal muscle. This enzyme catalyzes the reversible phosphorylation of creatine by ATP to creatine phosphate and ADP . Creatine phosphate is the major source of high energy phosphate used in muscle contraction.

  • Page 67: Creatinine/Crea

    Chemistry Description and Guide Creatinine/CREA Creatinine is a degradation product of creatine in muscle metabolism. The daily production of creatinine is fairly constant and not influenced markedly by age, diet, exercise, or catabolism. Creatinine is eliminated from the body by glomerular filtration and tubular secretion in the kidneys.
  • Page 68 IDEXX VetTest* Chemistry Analyzer Operator’s Manual Gamma-glutamyltransferase/GGT quantities in the kidney medulla and cortex and to a lesser extent in the small intestinal mucosa and bile canaliculi. (See Enzymes does not result in high enzyme activity in the serum sample.

  • Page 69: Glucose/Glu

    Chemistry Description and Guide Glucose/GLU Glucose is the principal source of energy in monogastric mammals. The circulating concentration in the healthy animal is maintained within narrow limits. Principal reason for performing the test: To investigate carbohydrate metabolism. Most common abnormality indicated by the test: Diabetes mellitus Special precautions in sample collection: sampling.

  • Page 70: Lactic Acid (Lac

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Lactic Acid (LAC) Lactate is produced by anaerobic metabolism of glucose and its concentration depends on relative rates of production in muscle cells and erythrocytes and metabolism in the liver. Principal reason for performing the test: Elevated lactate levels usually are caused by overproduction or undermetabolism.

  • Page 71: Lactate Dehydrogenase/Ldh

    Chemistry Description and Guide Lactate dehydrogenase/LDH The enzyme lactate dehydrogenase is present in large amounts in all organs and tissues (including red blood cells) of dogs and cats. It is found in the cell cytoplasm and is released into the blood during changes in cell membrane permeability or necrosis.

  • Page 72: Lipase/Lipa

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Lipase/LIPA Important: Read this page in conjunction with the details on Amylase (AMYL). The enzymes lipase and amylase are found at high activity in the pancreas and to a lesser extent in the salivary glands, small intestinal mucosa, and liver. They have a short may be used in the confirmation of acute pancreatitis, but because of the slightly longer...

  • Page 73: Magnesium/Mg

    Chemistry Description and Guide Magnesium/Mg Magnesium plays an important intracellular role in the activation of enzymes, including those responsible for many anabolic and catabolic processes. It is also involved in the formation and destruction acetylcholine, which governs the transmission of electrical impulses at the neuromuscular junction.

  • Page 74: Ammonia/Nh

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Ammonia/NH Ammonia is the catabolic product of protein digestion and is extremely toxic. It is converted rapidly in the liver to urea which is eliminated from the body by the kidneys. Principal reason for performing the test: To detect a portosystemic shunt.

  • Page 75: Inorganic Phosphate/Phos

    Chemistry Description and Guide Inorganic phosphate/PHOS Phosphorus is an element which plays a major role as a metabolic intermediate and is a constituent of nucleic acids, phospholipids, and nucleotides. Phosphates are also important components of buffering systems within the body fluids. Phosphate and calcium are absorbed in the small intestine.

  • Page 76: Total Bilirubin/Tbil

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Total bilirubin/TBIL Hemoglobin from degenerate erythrocytes is converted to bilirubin in the reticuloendothelial system. Free unconjugated bilirubin is transported to the liver bound to albumin where it is conjugated with glucuronic acid and eliminated in the bile.

  • Page 77: Total Protein/Tp

    Chemistry Description and Guide Total protein/TP The serum total protein concentration comprises all the proteins found in the aqueous phase of the blood. In the healthy animal, albumin constitutes the major single component. The remaining proteins are the alpha, beta, and gamma globulins. The globulin concentration is determined by subtracting the albumin from the total protein.

  • Page 78: Triglycerides/Trig

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Triglycerides/TRIG Triglycerides are usually present in the diet of dogs and cats, especially when the animals are fed table scraps. They are also synthesized in the liver, mainly from carbohydrates providing a secondary energy source, and are stored in fatty tissue.

  • Page 79: Urea/Urea/Bun

    Chemistry Description and Guide Urea/UREA/BUN The catabolism of proteins results in the production of ammonia which is extremely toxic. This is converted to urea in the liver and eliminated from the body by glomerular filtration in the kidneys. Principal reason for performing the test: As an indicator of renal disease.

  • Page 80: Uric Acid/Uric

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Uric acid/URIC Uric acid determinations are useful in avian patients and Dalmation dogs in place of urea determinations. In all dogs (except Dalmations) with diffuse hepatic disease, there is marked elevation of blood uric acid above the normal levels of <1 mg/dL.

  • Page 81: Urine Creatinine

    Chemistry Description and Guide Urine Creatinine Urine creatinine is determined so the concentration of electrolytes filtered or lost through the glomeruli or renal tubules such as urinary protein or cortisol can be quantitated, compared, and expressed as ratios with diagnostic significance. Principal reason for performing the test: To be performed with urine protein in order to determine the urine protein to creatinine ratio (UPC).

  • Page 82: Urine Protein/Upro

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Urine Protein/UPRO Urinary protein is determined and compared to the concentration of creatinine in order to assess the level of renal protein (glomeruli and tubular) loss to determine the urine protein/creatinine ratio (UPC). Principal Reason for Performing the Test To be performed with urine creatinine in order to determine the urine protein to creatinine ratio (UPC).

  • Page 83: Upc Protocol

    Chemistry Description and Guide UPC Protocol Principle reason for performing test: amyloidosis and as an early marker of chronic renal failure. Includes: Urine protein, urine creatinine, protein:creatinine ratio Submission Requirements: 2 mL urine in a sterile container Storage/Stability: Interferences: Gross hematuria, pyuria. Complementary tests include complete urinalysis with culture and sensitivity.
  • Page 84 IDEXX VetTest* Chemistry Analyzer Operator’s Manual H–28...

  • Page 85: I Self-Help Guide

    Solution: Reboot the VetTest analyzer using another disk. Cause: The disk drive is damaged or dirty. Solution: Turn off the VetTest analyzer. On the back of the VetTest analyzer, remove the metal cover over the software disk (see Connecting the Power Cord and Turning On the Analyzer Turn on the VetTest analyzer and observe the light above the electrical connection.
  • Page 86 Cause: The connection between the keyboard and the VetTest analyzer is faulty. Solution: Turn off the VetTest analyzer, remove and reinsert the cable connection on the back of the analyzer, and then turn on the VetTest analyzer. Analyzer returns to Startup during analysis.
  • Page 87 VetTest analyzer from the bottom of the roll. Cause: The paper is jammed in printer. Solution: Turn off the VetTest analyzer and remove the paper roll. Carefully remove any pieces of paper visible in the slot. analyzer. Note: You can still use the VetTest analyzer when the printer is not working.

  • Page 88: Screen "Failure" Messages

    The slide insertor tray was pushed in accidentally during analysis. Solution: Repeat the analysis. Do not push the slide insertor tray in during analysis. Note: Shut down and restart the VetTest analyzer if the abort was caused by the slide insertor tray hitting the rotor.
  • Page 89 The software version is not current. Solution: Make sure the latest software is in use. Updated software is routinely mailed and should be inserted immediately upon receipt. Turn off the VetTest analyzer, Call IDEXX Technical Support if your latest version still does not work.
  • Page 90 Important: Ensure the rotor cover is refitted correctly. Light entering the analyzer could interfere with the result readings. 8. Turn on the VetTest analyzer to reset the rotor to its proper position. Note: If no physical obstruction is noted and rotor knob is tight, slide chute may need adjustment or ejector arm may be stuck.
  • Page 91 Screen display reads: “Results invalidated. Slide spotting failure.” Cause: Sample wicking up the outside of the pipettor tip during slide spotting. Solution: Rerun the sample with new slides and thoroughly wipe the pipettor tip with a twisting motion all the way down to the end (see Preparing the Pipettor for a Sample, Note: not a generic version.
  • Page 92 Air leak in the pipettor line. Solution: Ensure there is a tight connection for the luer–lock syringe connector. Inspect the tubing for holes and replace the tube if necessary (see VetTest Pipettor, Note: Even though the VetTest analyzer may show only one result as invalid, the other results are suspect.
  • Page 93 Temperature Warnings Problem: The VetTest analyzer takes more than 25 minutes to warm up. Cause: The VetTest analyzer cannot reach the proper operating temperature due to a cold or hot room, or analyzer temperature. The room temperature Note: If the temperature is not correct, the VetTest analyzer will change the warm–up cycle until it reaches the proper temperature.

  • Page 94: Miscellaneous

    Result differs with commercial laboratory or other instrument result. Solution: Comparing results using different equipment or methods is imprecise at best. (Review Appendix: Differences in Results QC procedure for that particular chemistry to rule out a VetTest analyzer or slide issue. Cause: Result differs with your expectations.

  • Page 95: J Specifications And Warnings

    Use only the power cable that is supplied with the unit. The VetTest analyzer contains moving parts that are accessible to the user during cleaning. Disconnect the power before cleaning. Refer to the cleaning instructions in this manual for the correct procedure.
  • Page 96 IDEXX VetTest* Chemistry Analyzer Operator’s Manual J–2...

  • Page 97: K Appendix: Differences In Results

    Reference ranges must be created for each analyte and each new instrument or method of analysis. Every commercial laboratory must establish its own species reference ranges for the equipment and methodology used. IDEXX is continually doing this work for you with every software release (see Your Participation in Reference Range Data Collection using different equipment or methods is imprecise at best.
  • Page 98 IDEXX VetTest* Chemistry Analyzer Operator’s Manual K–2...

  • Page 99: Appendix: Reference Ranges

    Appendix: Reference Ranges Canine—Puppies Test U.S. Units S.I. Units *ALB g/dL ALKP AMYL 300–1300 U/L 300–1300 U/L mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA 0.3–1.2 mg/dL 0–2 U/L 0–2 U/L mg/dL mmol/L mmol/L mmol/L LIPA mg/dL mmol/L PHOS mg/dL mmol/L TBIL 0–0.8 mg/dL...

  • Page 100: Canine-Adults

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Canine—Adults Test U.S. Units S.I. Units *ALB g/dL ALKP 23–212 U/L 23–212 U/L 10–100 U/L 10–100 U/L AMYL mg/dL mmol/L mg/dL mmol/L CHOL 110–320 mg/dL mmol/L 10–200 U/L 10–200 U/L CREA mg/dL mg/dL mmol/L...

  • Page 101: Canine-Geriatric

    Appendix: Reference Ranges Canine—Geriatric Test U.S. Units S.I. Units *ALB g/dL ALKP 23–212 U/L 23–212 U/L 10–100 U/L 10–100 U/L AMYL mg/dL mmol/L mg/dL mmol/L CHOL 110–320 mg/dL mmol/L 10–200 U/L 10–200 U/L CREA mg/dL mg/dL mmol/L mmol/L mmol/L LIPA 200–1800 U/L 200–1800 U/L mg/dL...

  • Page 102: Feline-Kittens

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Feline—Kittens Test U.S. Units S.I. Units g/dL ALKP AMYL 0–32 U/L 0–32 U/L mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL 0–1 U/L 0–1 U/L mg/dL mmol/L mmol/L mmol/L 0–1128 U/L 0–1128 U/L...

  • Page 103: Feline-Adults

    Appendix: Reference Ranges Feline—Adults Test U.S. Units S.I. Units g/dL ALKP 12–130 U/L 12–130 U/L AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL 0–1 U/L 0–1 U/L mg/dL mmol/L mmol/L mmol/L LIPA mg/dL mmol/L PHOS mg/dL mmol/L TBIL mg/dL g/dL TRIG...

  • Page 104: Feline-Geriatric

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Feline—Geriatric Test U.S. Units S.I. Units g/dL ALKP 12–130 U/L 12–130 U/L AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL 0–1 U/L 0–1 U/L mg/dL mmol/L mmol/L mmol/L LIPA mg/dL mmol/L PHOS...

  • Page 105: Equine-Yearlings

    Appendix: Reference Ranges Equine—Yearlings Test U.S. Units S.I. Units g/dL ALKP AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L mmol/L mmol/L LIPA mg/dL mmol/L PHOS mg/dL mmol/L TBIL mg/dL g/dL TRIG mg/dL mmol/L GLOB g/dL mmol/L mmol/L mmol/L mmol/L...

  • Page 106: Equine-Foals

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Equine—Foals Test U.S. Units S.I. Units g/dL ALKP AMYL 0–10 U/L 0–10 U/L 0–228 U/L 0–228 U/L mg/dL mmol/L mg/dL mmol/L CHOL mg/dL 2.02–11.83 mmol/L CREA mg/dL mg/dL mmol/L mmol/L mmol/L 0–1830 U/L 0–1830 U/L LIPA 200–1300 U/L...

  • Page 107: Equine-Adults

    Appendix: Reference Ranges Equine—Adults Test U.S. Units S.I. Units g/dL ALKP AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA 0.8–2.2 mg/dL mg/dL mmol/L mmol/L mmol/L LIPA mg/dL mmol/L PHOS mg/dL mmol/L TBIL mg/dL g/dL TRIG mg/dL mmol/L GLOB g/dL mmol/L mmol/L mmol/L...

  • Page 108: Equine-Mares At Stud

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Equine—Mares at Stud Test U.S. Units S.I. Units g/dL ALKP 0–20 U/L 0–20 U/L AMYL 0–8 U/L 0–8 U/L 0–333 U/L 0–333 U/L mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L...

  • Page 109: Dairy Cows

    Appendix: Reference Ranges Dairy Cows Test U.S. Units S.I. Units g/dL ALKP 28–233 U/L 28–233 U/L AMYL mg/dL mmol/L 8–12 mg/dL 2–3 mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L LIPA 30–200 U/L 30–200 U/L 1.8–3.0 mg/dL 0.8–1.3 mmol/L PHOS mg/dL 1.3–2.8 mmol/L TBIL...

  • Page 110: Beef Cattle

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Beef Cattle Test U.S. Units S.I. Units g/dL ALKP AMYL 0–28 U/L 0–28 U/L mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L 0–110 U/L 0–110 U/L CREA mg/dL 0–80 U/L 0–80 U/L mg/dL mmol/L LIPA...

  • Page 111: Llama

    Appendix: Reference Ranges Llama Test U.S. Units S.I. Units g/dL ALKP AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L mg/dL mmol/L PHOS 1.0–11.0 mg/dL mmol/L TBIL 0.1–0.2 mg/dL 2–3 g/dL GLOB g/dL mmol/L mmol/L mmol/L mmol/L Cl–...

  • Page 112: Sea Turtles

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Sea Turtles Test U.S. Units S.I. Units g/dL ALKP BUN/UREA mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA 0.1–0.3 mg/dL GLOB g/dL mg/dL mmol/L 100–800 U/L 100–800 U/L mg/dL mmol/L PHOS mg/dL mmol/L g/dL...

  • Page 113: Provisional Reference Ranges

    Appendix: Reference Ranges Provisional Reference Ranges Avian—Parrots Amazon U.S. Test Amazon Blue Yellow Eclectus African Grey Units g/dL ALKP 2–21 AMYL 28–200 mg/dL 8.13–11.31 mg/dL CHOL mg/dL CREA 0.00–0.10 0.00–0.11 mg/dL 1–12 0–1 mg/dL LIPA 1.81–3.08 mg/dL PHOS mg/dL TBIL 0.00–0.01 0.00–0.13 0.00–0.10...

  • Page 114: Avian-Cockatoos

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Avian—Cockatoos Test Grey Cheek Moluccan Umbrella U.S. Units g/dL ALKP 0–3000 0–8 AMYL mg/dL 8.33–11.10 mg/dL CHOL mg/dL 100–300 CREA mg/dL 0–1 0–3 mg/dL LIPA mg/dL 103–318 PHOS mg/dL TBIL 0.00–0.30 mg/dL g/dL TRIG...

  • Page 115: Avian-Budgerigar

    Appendix: Reference Ranges Avian—Budgerigar Test Budgerigar U.S. Units g/dL ALKP AMYL mg/dL mg/dL CHOL mg/dL CREA mg/dL mg/dL LIPA mg/dL PHOS mg/dL TBIL mg/dL 2.00–3.00 g/dL TRIG mg/dL URIC mg/dL GLOB 2.10–3.20 g/dL...

  • Page 116: Avian-Cockatiel, Canary, Conure

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Avian—Cockatiel, Canary, Conure Test Cockatiel Canary Conure U.S. Units g/dL ALKP 0–3000 0–3000 0–3000 AMYL mg/dL mg/dL CHOL mg/dL 100–300 100–300 CREA mg/dL mg/dL LIPA mg/dL PHOS mg/dL TBIL mg/dL g/dL TRIG mg/dL URIC 2.10–3.20...

  • Page 117: Avian—Macaws

    Appendix: Reference Ranges Avian—Macaws Test Blue and Gold Blue Hyacinth Scarlet U.S. Units g/dL ALKP 0–11 3–8 AMYL mg/dL mg/dL CHOL mg/dL CREA 0.10–0.13 mg/dL 0–1 0–1 0–1 mg/dL LIPA 1.33–2.80 mg/dL PHOS mg/dL TBIL 0.00–1.02 mg/dL g/dL TRIG mg/dL URIC mg/dL...

  • Page 118: Ferret

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Ferret Test U.S. Units S.I. Units g/dL ALKP 28–120 IU 28–120 IU mg/dL mmol/L 8.0–11.8 mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L PHOS mg/dL mmol/L TBIL 0.1–1.0 mg/dL g/dL TRIG mg/dL mmol/L GLOB 1.8–3.1 g/dL...

  • Page 119: Goat

    Appendix: Reference Ranges Goat Test U.S. Units S.I. Units 2.8–3.8 g/dL 28–38 g/L ALKP AMYL 1–30 IU 1–30 IU 122–321 IU 122–321 IU 10–21 mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L 28–130 IU 28–130 IU CREA mg/dL mg/dL mmol/L PHOS mg/dL mmol/L TBIL...

  • Page 120: Monkey

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Monkey Test U.S. Units S.I. Units g/dL ALKP 20–120 IU 20–120 IU AMYL mg/dL mmol/L 8.3–10.1 mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L PHOS mg/dL 0.8–2.1 mmol/L TBIL mg/dL g/dL TRIG mg/dL mmol/L L–22...

  • Page 121: Mouse

    Appendix: Reference Ranges Mouse Test U.S. Units S.I. Units g/dL ALKP 28–132 IU 28–132 IU AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA 0.2–0.8 mg/dL mg/dL mmol/L PHOS mg/dL 2.0–3.3 mmol/L TBIL mg/dL g/dL TRIG mg/dL mmol/L URIC mg/dL 101–321 L–23...

  • Page 122: Pig

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Test U.S. Units S.I. Units 1.8–33.0 g/dL 18–330 g/L ALKP AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L LIPA PHOS mg/dL 1.2–3.0 mmol/L TBIL 0.1–0.3 mg/dL g/dL TRIG mg/dL mmol/L...

  • Page 123: Rabbit

    Appendix: Reference Ranges Rabbit Test U.S. Units S.I. Units g/dL ALKP mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA 0.8–1.8 mg/dL mg/dL mmol/L PHOS mg/dL mmol/L TBIL 0.3–0.8 mg/dL g/dL TRIG mg/dL mmol/L GLOB g/dL...

  • Page 124: Rat

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Test U.S. Units S.I. Units g/dL ALKP AMYL mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L LIPA PHOS mg/dL mmol/L TBIL mg/dL g/dL TRIG mg/dL mmol/L GLOB g/dL URIC mg/dL...

  • Page 125: Sheep

    Appendix: Reference Ranges Sheep Test U.S. Units S.I. Units g/dL ALKP AMYL 1–30 IU 1–30 IU mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L 8–100 IU 8–100 IU CREA mg/dL mg/dL mmol/L LIPA 2.3–3.0 mg/dL mmol/L PHOS mg/dL mmol/L TBIL mg/dL g/dL TRIG mg/dL...

  • Page 126: Snake

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Snake Test U.S. Units S.I. Units ALKP 1–11 mg/dL mmol/L 10–22 mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL 10–108 mg/dL mmol/L PHOS mg/dL mmol/L g/dL TRIG mg/dL mmol/L URIC 1–10 mg/dL L–28...

  • Page 127: Tortoise

    Appendix: Reference Ranges Tortoise Test U.S. Units S.I. Units 1.3–3.0 g/dL 13–30 g/L ALKP mg/dL mmol/L mg/dL mmol/L CREA mg/dL PHOS mg/dL mmol/L TBIL mg/dL g/dL GLOB g/dL URIC 1.2–2.8 mg/dL...

  • Page 128: Lizard

    IDEXX VetTest* Chemistry Analyzer Operator’s Manual Lizard Test U.S. Units S.I. Units ALKP mg/dL mmol/L mg/dL mmol/L CHOL mg/dL mmol/L CREA mg/dL mg/dL mmol/L PHOS mg/dL mmol/L g/dL TRIG mg/dL mmol/L URIC mg/dL L–30...

  • Page 129: M Appendix: Unit Conversion

    Appendix: Unit Conversion Conversion Factors Conversion Factors Units Chemistry U.S. Conc. Unit U.S. to S.I. U.S. to French S.I. Conc. Unit French Conc. Unit mg/dL 0.01 mmol/L mg/dl 0.021 mmol/L mg/dL mmol/L mg/L URIC mg/dL mg/L g/dL TRIG mg/dL 0.01 nmol/L CHOL mg/dL...
  • Page 130 IDEXX VetTest* Chemistry Analyzer Operator’s Manual M–2...
  • Page 132 One IDEXX Drive Westbrook, Maine 04092 USA idexx.com 06-02247-05...

Table of Contents

Save PDF