Idexx Catalyst One Operator's Manual
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Idexx Catalyst One Operator's Manual

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IDEXX Catalyst One
Chemistry Analyzer
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Operator's Guide

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Summary of Contents for Idexx Catalyst One

  • Page 1 IDEXX Catalyst One Chemistry Analyzer Operator’s Guide...
  • Page 2 *IDEXX VetLab, Catalyst, Catalyst One, IDEXX VetTrol, SmartLink, IDEXX InterLink, IDEXX SmartService, SNAP, and 4Dx are trademarks or registered trademarks of IDEXX Laboratories, Inc. in the United States and/or other countries. All other product and company names and logos are trademarks of their respective...

  • Page 3: Table Of Contents

    Entering Standby Mode ..........................18 Exiting Standby Mode..........................18 Sample Preparation and Storage ......................19 Supported Sample Types for Catalyst* CLIPs and Slides.................19 Preparing Samples for Use on the Catalyst One Analyzer................20 Proper Sample Cup Volume ........................22 Sample Inspection After Centrifugation .....................22 Sample Storage ............................23 Quality Control ............................24...
  • Page 4 Cleaning the Outside of the Analyzer and the Sample Drawer ........................29 Emptying the Waste Drawer ........................29 Appendices ............................30 Chemistry Descriptions ..........................30 Medical Protocol Descriptions ........................51 Profile Selection ............................56 Differences in Results ..........................57 Technical Specifications ..........................57 IDEXX Technical Support Contact Information ..................58...

  • Page 5: Preface

    The analyzer does not contain any user-serviceable components. DO NOT disassemble. Line voltage for the Catalyst One AC power adapter is 100–240 V AC, 50–60 Hz. Be sure to plug all equipment into properly grounded electrical outlets.

  • Page 6: International Symbol Descriptions

    (such as expiration date, temperature limitations, batch code, etc.). IDEXX Laboratories has adopted the use of international symbols on our analyzers, product boxes, labels, inserts, and manuals in an effort to provide our users with easy-to-read information.

  • Page 7: Other Symbols

    Preface Symbol Description Symbol Description Caution, hot surface Electrostatic-sensitive device Attention, surface très chaude Appareil sensible aux charges Precaución, superficie caliente éléctrostatiques Vorsicht, heiße Oberfläche Dispositivo sensible a descargas Attenzione, superficie rovente electrostáticas 高温注意 Gerät ist sensibel auf elektrostatische Ladung Dispositivo sensibile alle scariche elettrostatiche 静電気の影響を受ける装置...

  • Page 8: Getting Started

    The Catalyst One analyzer is for veterinary use only. IDEXX VetLab* Station Connectivity The Catalyst One analyzer is part of the IDEXX VetLab* suite of analyzers, all of which connect to the IDEXX VetLab Station (IDEXX’s laboratory information management system). Connecting multiple analyzers to the IDEXX VetLab Station helps you attain a comprehensive picture of your patient’s health, with the ability to view test results from multiple analyzers on a single report,...

  • Page 9: Catalyst One Components

    There are several important steps that the analyzer performs in order to present the results of a sample. Once the slides and sample have been inserted into the analyzer, the Catalyst One analyzer incubates the slides. Then, if using a Catalyst* whole blood separator, the plasma is separated from a whole blood sample.

  • Page 10: Analyzer Status

    Analyzer Status The light-emitting diode (LED) indicator on the front panel of the Catalyst One analyzer indicates the analyzer’s status. Note: You can also view the analyzer status by viewing its icon on the IDEXX VetLab Station Home screen. LED Color...

  • Page 11: Responding To An Alert

    When the analyzer experiences a problem, an alert message appears on the upper right side of the IDEXX VetLab Station title bar, the LED on the front panel of the Catalyst One analyzer flashes red, and the Catalyst One icon on the IDEXX VetLab Station Home screen appears with an Alert status.

  • Page 12: Catalyst One Analyzer Consumables

    Getting Started Catalyst One Analyzer Consumables The following consumables are available for use with the Catalyst One analyzer: CLIPs, Panels, and Slides Chemistry Abbreviation Albumin       Alkaline Phosphatase ALKP      ...

  • Page 13: Compatible Species

    Getting Started Compatible Species Canine Ferret Rabbit † Feline Goat † Equine Lizard Sea Turtles † Bovine (beef cattle, dairy cow) Llama Sheep Avian (budgerigar, cockatoos Monkey Snake [grey cheek, Moluccan, umbrella], Mouse Tortoise cockatiel, canary, conure, macaw [blue and gold, hyacinth, scarlet], parrots [Amazon blue, Amazon yellow, eclectus, African grey]) Species-specific intervals are available for these species.

  • Page 14: Using The Catalyst One Analyzer

    For more information on these work flows, see the IDEXX VetLab Station Operator’s Guide. Slide Handling The Catalyst One analyzer allows you to run up to 25 tests on a single sample. Before you begin, please take note of the following: •...
  • Page 15 Use the proper diluent material for your sample type. – For plasma and serum samples, use normal saline. – IDEXX does not recommend manually diluting whole blood in a Catalyst* whole blood separator—only dilute the separated plasma. – For urine, use Catalyst* Urine P:C Diluent.

  • Page 16: Viewing And Printing Test Results

    Patient test results can be printed automatically each time a set of results are returned or you can manually print the results when needed. For more information about how to view and print test results, see the IDEXX VetLab Station Operator’s Guide.

  • Page 17: Outside Of Reportable Range Samples

    Using the Catalyst One* Analyzer Outside of Reportable Range Samples Occasionally a test value may be outside the analyzer’s reportable range capability. The test value may be greater than (“>”) the reportable range, or interfering substances in the sample may be causing a nonlinear or invalid result.

  • Page 18: Modifying The Settings On The Analyzer

    The analyzer will beep when it encounters an alert. You can modify the Sound settings to turn the sound off or adjust its volume. Tap Instruments on the IDEXX VetLab Station Home screen. 2. Tap the Catalyst One side tab.

  • Page 19: Sample Preparation And Storage

    Sample Preparation and Storage Supported Sample Types for Catalyst* CLIPs and Slides The following sample types can be used with Catalyst* CLIPs and slides: CLIPs/Slides Abbreviation Chem 17 CLIP    Chem 15 CLIP    Chem 10 CLIP ...

  • Page 20: Preparing Samples For Use On The Catalyst One Analyzer

    Sample Preparation and Storage Preparing Samples for Use on the Catalyst One Analyzer You can run untreated whole blood, lithium heparinized whole blood, plasma, serum, and urine samples on the Catalyst One analyzer. IMPORTANT: Do not use EDTA or sodium heparin for chemistry analysis.
  • Page 21 Sample Preparation and Storage To Prepare a Serum Sample Use the appropriate tube and collection device. 2. Draw the sample gently and transfer if necessary. 3. Let the sample clot for a minimum of 20 minutes. 4. Centrifuge the sample. 5.

  • Page 22: Proper Sample Cup Volume

    Sample Preparation and Storage Proper Sample Cup Volume The volume of plasma, serum, or urine sample required varies based on the number of slides being used in the run: Number of slides Sample cup fill volume (µL) Sample Inspection After Centrifugation It is good practice to examine the sample carefully following centrifugation in a centrifuge and/or in the analyzer (by running a whole blood separator).

  • Page 23: Sample Storage

    (Na, K, Cl), and glucose (GLU) chemistry descriptions for additional special handling and storage requirements. • IDEXX does not recommend freezing samples that will be used to run electrolytes. Storing Whole Blood Lithium heparinized whole blood samples should be analyzed immediately. Samples that are not analyzed within 30 minutes should be placed in a tube to be separated and stored.

  • Page 24: Quality Control

    Quality Control Materials IDEXX VetTrol* Control In each box of IDEXX VetTrol* Control, there are four vials containing freeze-dried powder (dark brown bottle marked “VetTrol Control”) and four vials containing diluent (lighter bottles marked “Diluent for VetTrol”). The lot numbers for the diluent and the control are different and can be found on the product packaging.

  • Page 25: Quality Control Clips And Slides

    Use the convenient QC CLIP and the Lyte 4 CLIP in conjunction with the IDEXX VetTrol Control fluid to perform quality control on your Catalyst One analyzer. It is recommended that you wait at least 30 minutes after running any slides before running the QC CLIP.

  • Page 26: Preparing Control Fluid

    Reconstitution, with occasional inversion, will take 45–60 minutes. Visually verify that all freeze-dried material is dissolved before using. Run quality control on the Catalyst One analyzer (see instructions below). To Prepare UPRO Control Fluid Take one vial of UPRO Control out of the refrigerator and gently invert it 6–10 times to mix thoroughly.

  • Page 27: Running Quality Control

    Running Quality Control To Run General Quality Control on the Catalyst One analyzer Tap the Catalyst One icon on the IDEXX VetLab Station Home screen. 2. Tap Maintenance and then tap Quality Control. 3. Tap the quality control lot number you are using and then tap Run QC.

  • Page 28: Maintenance

    Upgrade the software promptly. Upgrading the Software As new features and functionality are added to the Catalyst One analyzer, you will receive software upgrades from IDEXX. If you have IDEXX SmartService* Solutions, the upgrade will be sent via your IDEXX VetLab* Station automatically. If you do not have IDEXX SmartService Solutions, you will receive an upgrade disc in the mail.

  • Page 29: Cleaning The Outside Of The Analyzer And The Sample Drawer

    Maintenance d. Using an IDEXX-supported alcohol prep pad, wipe the incubator ring and optics window in a counterclockwise direction. Repeat this step at least three times using a new alcohol prep pad for each wipe. e. Clean the white reference tile using a new alcohol prep pad.

  • Page 30: Appendices

    A medical review board has approved the content presented in this document. IDEXX has more than 40 reference laboratories worldwide employing over 100 veterinarians. If you have any questions about the medical content or interpretation of results in this document, please contact IDEXX Laboratories.
  • Page 31 Appendices Reaction Sequence Albumin (ALB) Albumin forms the largest fraction of the total serum protein in the healthy animal. It is synthesized solely by the liver, has a relatively low molecular weight, and plays an important role in the transport of endogenous and exogenous compounds by binding with those compounds.
  • Page 32 Appendices Principal Reason for Performing the Test As an indicator of hepatic and/or biliary disease. Most Common Abnormality Indicated by the Test Obstructive changes in the biliary system. A special consideration for interpreting ALKP changes in the dog is required because there are “induced” forms of ALKP due to glucocorticoids and other influences that are not associated with the natural tissue sources of ALKP.
  • Page 33 Appendices Complementary Tests Ammonia may be determined in isolation but more often in conjunction with other tests of hepatic damage or dysfunction, such as pre- and postprandial bile acids. Reaction Sequence Amylase (AMYL) This section should be read in conjunction with the Lipase (LIPA) section. The main source of serum amylase is the pancreas, although pathology of the liver and small intestine may result in significant elevations of this enzyme (above the reference range).
  • Page 34 Appendices Most Common Abnormalities Indicated by the Test Dogs and cats—cardiac or skeletal muscle injury when ALT is not increased; liver, cardiac, or skeletal muscle injury if both ALT and AST are increased. Horses, cows, and pigs —liver, cardiac, or skeletal muscle injury. Sample Type and Precautions Remove plasma or serum promptly from the cells or clot.
  • Page 35 Appendices Complementary Tests Urea concentration should usually be determined in conjunction with measurements of creatinine, inorganic phosphate, total protein, albumin, and a complete urinalysis. Urea concentration is influenced by high-protein diet rather than creatinine. Reaction Sequence Calcium (Ca) Calcium is an essential element that is involved in many body systems. These include the skeleton, enzyme activation, muscle metabolism, blood coagulation, and osmoregulation.
  • Page 36 Avoid hemolysis—sample should be run as soon as possible after serum or plasma is separated from the cells or clot. If plasma is being collected, use only lithium heparinized samples. Do not freeze samples for use with the Catalyst One analyzer. Complementary Tests Sodium, potassium, and chloride should always be assayed together to determine electrolyte balance.
  • Page 37 Appendices Reaction Sequence Creatine Kinase (CK) Creatine kinase is found at high activity only in the cytoplasm of cardiac and skeletal muscle. This enzyme catalyzes the reversible phosphorylation of creatine by ATP to creatine phosphate and ADP. Creatine phosphate is the major source of high-energy phosphate used in muscle contraction. Principal Reason for Performing the Test To identify injury to skeletal or cardiac muscle.
  • Page 38 Appendices Creatinine (CREA) Creatinine is a degradation product of creatine in muscle metabolism. The daily production of creatinine is fairly constant and not influenced markedly by age, diet, exercise, or catabolism. Creatinine is eliminated from the body by glomerular filtration and tubular secretion in the kidneys. Principal Reasons for Performing the Test As an indicator of renal disease and/or an index of glomerular filtration rate.
  • Page 39 Serum is preferred for fructosamine testing as customer experience shows that it more consistently provides good quality samples. Examine the serum or plasma for hemolysis. Although IDEXX dry-slide technology dramatically reduces the effect of this interfering substance, marked hemolysis can result in inaccurate fructosamine results.
  • Page 40 Appendices Reaction Sequence Glucose (GLU) Glucose is the principal source of energy in monogastric mammals. The circulating concentration in the healthy animal is maintained within narrow limits. Principal Reason for Performing the Test To investigate carbohydrate metabolism. Most Common Abnormality Indicated by the Test Increased glucose—diabetes mellitus;...
  • Page 41 Appendices Principal Reason for Performing the Test As a measure of glomerular filtration rate. Most Common Abnormality Indicated by the Test Increased inorganic phosphate—decreased glomerular filtration. Sample Type and Precautions Remove plasma or serum promptly from the cells or clot. If plasma is being collected, use only lithium heparinized samples.
  • Page 42 Appendices Reaction Sequence Lactate (LAC) Lactate is produced by anaerobic metabolism of glucose and its concentration depends on relative rates of production in muscle cells and erythrocytes and metabolism in the liver. Principal Reason for Performing the Test Elevated lactate levels usually are caused by overproduction or under metabolism. They result from tissue hypoxia, diabetes mellitus, malignancies, ethanol or methanol ingestion, and metabolic acidosis.
  • Page 43 Appendices Complementary Tests Lipase and amylase are usually determined in conjunction with tests of hepatic and pancreatic function or damage. Canine and feline pancreas-specific lipase tests should be performed in questionable cases. Reaction Sequence Magnesium (Mg) Magnesium plays an important intracellular role in the activation of enzymes including those responsible for many anabolic and catabolic processes.
  • Page 44 Appendices Reaction Sequence Phenobarbital (PHBR) Phenobarbital is a commonly used drug used to treat seizures in a variety of species. Phenobarbital levels should be evaluated during initial dosing and throughout treatment to ensure that the blood levels are within the targeted therapeutic range. Principal Reasons for Performing the Test Phenobarbital is a controlled barbiturate medication that is used to treat veterinary patients that have seizures.
  • Page 45 Remove plasma or serum promptly from cells or clot. If plasma is being collected, use only lithium heparinized samples. Avoid hemolysis. Do not freeze samples for use with the Catalyst One analyzer. Complementary Tests Sodium, potassium, and chloride should always be assayed together to determine electrolyte balance.
  • Page 46 Appendices Complementary Tests Sodium, potassium, and chloride should always be assayed together to determine electrolyte balance. The additional measurement of bicarbonate will allow accurate assessment of metabolic acid-base physiology. Reaction Sequence Total Bilirubin (TBIL) Hemoglobin from degenerated erythrocytes is converted to bilirubin in the monocyte-macrophage system.
  • Page 47 Appendices Total Protein (TP) The serum total protein concentration comprises all the proteins found in the aqueous phase of the blood. In healthy animals, albumin is the major single component. The remaining proteins are the alpha, beta, and gamma globulins. The globulin concentration is determined by subtracting the albumin from the total protein.
  • Page 48 Appendices Hypothyroidism—a decreased TT is consistent with but not necessarily definitively diagnostic of hypothyroidism. Naturally occurring hypothyroidism is a common endocrine disorder in dogs and rare in cats. Hypothyroidism can be seen in horses as well. Nonthyroidal illness (NTI)—nonthyroidal illness can affect TT levels (and potentially other thyroid tests as well).
  • Page 49 Appendices Reaction Sequence Uric Acid (URIC) Uric acid determinations are useful in avian patients and dalmatians in place of urea determinations. In all dogs (except dalmatians) with diffuse hepatic disease, there is marked elevation of blood uric acid above the normal levels of <1 mg/dL. Principal Reason for Performing the Test As an indicator of the severity of renal disease in avian populations (and dalmatians).
  • Page 50 Appendices Principal Reason for Performing the Test To be performed with urine protein in order to determine the urine protein:creatinine ratio (UPC). Most Common Abnormality Indicated by the Test Proteinuria indicating early renal disease, protein-losing nephropathy. Sample Type and Precautions Urine, preferably collected through cycstocentesis, collected in a clean container.

  • Page 51: Medical Protocol Descriptions

    Appendices Complementary Tests Complete urinalysis with culture and sensitivity. Serum chemistries such as creatinine, BUN, albumin, and globulin. SNAP 4Dx Test Storage Information Handle and store urine samples in closed containers to avoid evaporation and contamination. Samples may be stored at room temperature for up to 4 hours. Refrigerated samples may be stored up to 3 days.
  • Page 52 Appendices Interpretation: Proteinuria requires proof of persistence and localization to prerenal, renal, or postrenal origins. Prove persistence of proteinuria by repeating the UPC ratio at least three times, a minimum of 2 weeks apart. • Prerenal proteinuria is possible when a CBC and a biochemical profile detect hemolysis, hyperglobulinemia or evidence of muscle damage.
  • Page 53 Appendices...
  • Page 54 Appendices Total T Protocols Canine hypothyroidism suspected Common clinical signs in dogs • Obesity Initial database • Skin disease • Total T • Lethargy • CBC • Mental dullness • Chemistry with • Exercise/Cold intolerance electrolytes • Complete urinalysis Low T with NTI Low T Low Normal T...
  • Page 55 Appendices Feline hyperthyroidism suspected Common clinical signs in cats • Weight loss Initial database • Hyperactivity • Total T • Polyphagia • CBC • Palpable goiter • Chemistry with • Unkempt coat electrolytes • Complete urinalysis Low T Normal T High T <0.8 µg/dL 0.8–4.7 µg/dL...

  • Page 57: Differences In Results

    For instance, a sample giving a Catalyst One* result that is slightly below the Catalyst One analyzer’s normal range should give a laboratory result slightly below the laboratory’s normal range.

  • Page 58: Idexx Technical Support Contact Information

    Appendices IDEXX Technical Support Contact Information IDEXX Sales Representative: Telephone/Voice Mail: United States United Kingdom IDEXX Laboratories, Inc. IDEXX Laboratories Ltd. One IDEXX Drive Riverside House, Riverside Walk Westbrook, Maine 04092 USA Windsor, Berkshire SL4 1NA Toll-Free Technical Support ..1-800-248-2483...
  • Page 59 Appendices Denmark IDEXX Laboratories Denmark ApS c/o Harbour House Sundkrogsgade 21 2100 Copenhagen, Denmark Telephone ........80 34 76 18 idexx.dk Finland IDEXX Laboratories Oy c/o Accounting Services Tilimatic Oy Mannerheimintie 16 A 3 00100 Helsinki, Finland Telephone ........0800 98458 idexx.fi...

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