CellaVision DM1200 User Manual page 162

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CellaVision
May Grünwald stain, stock solution:
May Grünwald working solution:
Buffer stock solutions 1 and 2:
Buffer working solution, pH 6.8
Giemsa stain, stock solution:
Giemsa working solution:
®
DM1200
Merck M1424 Eosin-methylenblue
solution, modified for microscopy
(contains methanol).
Store at +15 ºC to +25 ºC.
Dilute 1 part of May Grünwald stock
solution with 1 part buffer work solution.
Stable for 8 hours.
1) 9.07g KH
deionized water
Store at +4 ºC to +8 ºC.
2) 9.45 g Na
1000ml deionized water
Store at +4 ºC to +8 ºC.
Combine 127 ml KH
solution with 123 ml Na
solution ad 5000 ml deionized water.
Adjust to pH 6.8.
Store at +4 ºC to +8 ºC.
Durable for a month at +4 ºC.
Merck M9204 Azur-eosine-
methylenblue solution for microscopy
(contains methanol).
Store at +15 ºC to +25 ºC.
Durable for several months if kept in a
dark bottle.
Dilute 1 part Giemsa stock solution with
19 parts buffer work solution.
Stable for 8 hours.
PO (0.067M) ad 1000ml
2 4
HPO (0.067M) ad
2 4
PO stock
2 4
HPO stock
2 4
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User's Manual