Body Fluid Application - CellaVision DM1200 User Manual

1.3.2

Body Fluid Application

General Functionality
• Presents an image on a screen of every located cell or object;
• Organizes and suggests cell classification (preclassification) for the located
nucleated cells;
• Makes it possible to identify, confirm or modify (reclassification) the suggested
classification of the located cells;
• Presents an overview image.
Preclassification for Body Fluid
The system preclassifies the following WBC classes: Neutrophils, Eosinophils,
Lymphocytes, Macrophages (including Monocytes) and Other. Cells pre-classified
as Basophils, Lymphoma cells, Atypical lymphocytes, Blasts and Tumor cells are
automatically forwarded to the cell class Other.
Unidentified is a class for cells and objects which the system has pre-classified
with a low confidence level.
The system preclassifies the following non-WBCs: Smudge cells and Artefacts.
Non-WBCs are reported as number of cells or objects /100 WBCs.
WBC Reclassification for Body Fluid
Besides the cell classes mentioned above, the operator can reclassify cells into the
following cell classes: Not classed and 15 user defined cell classes.
Not classed should be used for cells and objects which the operator cannot identify
and wants to exclude from the differential count.
Sample Preparation
Body fluid samples are prepared by using a cytocentrifuge and a staining unit. The
sample is centrifuged onto a glass slide and stained with Romanovsky stain (see
Appendix G — Slide Preparation Guidelines for recommended staining recipes).
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CellaVision DM1200
User's Manual