What Do My Results Mean - Primerdesign genesig q16 Instruction Manual

Qpcr machine
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What do my results mean?

Analysis of your data is carried out automatically by the
genesig q16. The following information is designed to
help you fully understand a result or to troubleshoot:
"Positive"
Explanation
Your sample has produced a result. Your target of
interest is present and you can use the reported quantity/
percentage.
"Negative"
Explanation
Your sample has produced a negative result. The target
is not present in your sample.
"Test Contaminated"
Explanation
The Negative Control should be completely free of any
DNA/RNA. If you see this warning message it means
that at some point during the setup, the Negative Control
has been contaminated with DNA/RNA and has given
a positive signal. This contamination has invalidated
the test. The Positive Control and your test samples
are both possible sources of contaminating DNA/RNA.
The PCR tubes from previous runs will also contain
very high amounts of DNA so it is important that these
are carefully disposed of after each run is completed
and NEVER OPENED. It may be the case that your kits
have become contaminated which will lead to the same
problem occurring repeatedly.
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