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Summary of Contents for Primerdesign genesig q16
- Page 1 P r o d u c t s f o r R e a l - T i m e P C R The genesig ® Instruction Manual...
- Page 2 DNA testing to everyone. As well as being beautiful and highly portable, the q16 is powerful. In combination with the genesig Easy kit range the genesig q16 can be used to detect and quantify over 450 different DNA/RNA targets.
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Page 3: Table Of Contents
Connecting the machine and installing the software General operation of the instrument Opening the software and selecting a test type The main screen layout Results Exporting results What do my results mean? Troubleshooting... -
Page 4: Connecting The Machine And
Mac/PC. Ideally your Mac/PC should run the most recent version of the operating system. The genesig q16 will run on OS X version 10.7.3 and newer releases for Mac and Windows Vista or 7, 8, 8.1 and 10 for PC. -
Page 5: 2| General Operation
Standalone operation via a USB drive If you are planning to run the instrument in standalone mode no Ethernet cable connection is required. Instead a USB drive is loaded with the experiment setup data and then inserted in to the base of the q16 via an extension cable, which is supplied, to initiate a run. -
Page 6: 2| General Operation
Installation of software The software requires a JavaScript runtime environment to operate successfully. If your PC/Mac does not currently support a current Java run-time environment you will be prompted to download and install an update. Follow the onscreen instructions to download and install the update and then re-start your computer. -
Page 8: General Operation Of The Instrument
The lid is replaced by repeating this operation in reverse. The genesig q16 uses low profile 0.2ml reaction tubes that are supplied as part of each genesig EASY kit. Do not use alternative reaction tubes as this may damage your instrument. -
Page 10: 4| The Main Screen
Open the software Once installed double click on the q16 software icon to open the software. Click the ‘New’ button to begin a new test. -
Page 11: Selecting A Test Type
Selecting a test type Select the type of test you wish to perform: Species test Select this if you wish to use a genesig EASY speciation kit. This test will calculate the percentage of your sample that is a particular species e.g. you can calculate what percentage of your beefburger is horse meat. -
Page 12: The Main Screen Layout
The main screen layout There are 2 tabs in the view. One for setting up your test and a second where your results will be displayed after the analysis is complete: In the setup screen there are 5 simple panels to consider; 1. -
Page 13: 5| Results
Your test will be given a default name but this can be changed by clicking in the name field and entering new text. The Notes field allows you to make notes on your test if required. 2. Samples:... -
Page 14: 5| Results
The main screen layout 2. Samples cont... In this panel you can define how many samples you wish to test. Use the + button to add more, and the – button to remove them. You can alter the order of the samples using the up and down arrows. -
Page 15: 5| Results
You can perform multiple tests on the genesig q16. In this panel you can define how many tests you wish to perform. Use the + button to add more and the – button to remove them. You can alter the order of the tests using the up and down arrows. -
Page 16: 5| Results
Similarly the well-position of the Positive and Negative Controls are also defined automatically. Be sure to follow this carefully when loading your samples in to the instrument. -
Page 17: 5| Results
From the list select the q16 instrument that you are using and analysis will begin automatically. The run can be stopped at any time by clicking the “Abort Run” button. N.B. if you abort the run, discard your samples as they may be affected by the run beginning. -
Page 18: 5| Results
• Click “Configuration” at top right hand corner of software screen. • Select the “genesig q16” tab at the left hand side of the pop-up. • Click on the “Add” button now displayed at the bottom of the pop-up window. -
Page 19: 5| Results
• Primerdesign can provide your I.T. department with the recommended protocols for manually configuring the instrument connection. 6. Standalone operation from a USB drive... -
Page 20: 5| Results
The main screen layout 6. Standalone operation from a USB drive In order to conduct a successful USB run, please follow the steps below: • Setup your experiment as normal • Connect the USB stick to the PC • Click ‘Start Run’ •... -
Page 21: 5| Results
• The USB should not be removed until the run is completed as indicated by the q16 saying ‘analysis complete’ and the LED light indicators changing to rainbow colours. -
Page 24: Results
.USB file on the USB drive. This can be opened in the software on your Mac or PC. Insert the USB in to your Mac/PC. Open the genesig q16 software and click “Open” then change the ‘Files of Type’... -
Page 25: 5| Results
The results window has 2 further tabs. The first shows a summary of the results complete with automated analysis. The second gives a more detailed view showing the underlying raw data and is designed for advance users or for troubleshooting. Summary view: A text based summary of the entire analysis is displayed in the top left panel. -
Page 26: 5| Results
Results In the summary view the samples are listed exactly as you set them up in the previous screen. The status field shows the test result as a simple, automatically analysed status. Species test results When carrying out a genesig EASY speciation test quantitative analysis is carried out automatically. -
Page 27: 5| Results
The q16 also automatically calculates the sensitivity of the test. The quality of your DNA sample will affect the sensitivity of testing that is possible. With a good quality sample, highly sensitive testing is possible. With a poor quality sample then testing becomes less sensitive. -
Page 28: 5| Results
Results Detailed results view: The detailed results view shows the raw underlying data and is designed for advanced users and for troubleshooting. Amplification plots are visible in the top left panel. The drop down menu allows you to view the amplification plots derived from the test reaction or the Internal Extraction Control/Universal target reaction. -
Page 29: 5| Results
Zooming: Click the magnifying glass icon to select it. To zoom into the graph, move the mouse to the top left of the area you wish to zoom to. Hold down the left mouse button, and move the mouse to the bottom right of the area you wish to zoom to, and release. -
Page 30: Exporting Results
Exporting results Detailed results can be exported by clicking the export button. A choice of export formats are available. e.g. .pdf .csv. An image file version of the amplification plots can be exported by clicking the graph export icon. -
Page 32: What Do My Results Mean
What do my results mean? Analysis of your data is carried out automatically by the genesig q16. The following information is designed to help you fully understand a result or to troubleshoot: “Positive” Explanation Your sample has produced a result. Your target of interest is present and you can use the reported quantity/ percentage. - Page 33 Solutions Clean your working area using a commercial solution such as PCR Clean™ to ensure the area is DNA free at the start of your run and re-run the test. If the problem persists then the kit has become contaminated and it will have to be discarded and replaced with a new kit.
- Page 34 What do my results mean? “Sample preparation failed” Explanation The test has failed because the quality of the sample was not high enough. The Internal Extraction Control component identifies whether the sample has been prepared correctly or if the sample is of low quality. This error message means that this quality control test has failed and the sample is not fit for analysis.
- Page 35 “Positive result, poor quality sample” Explanation The test is positive so if you are only interested in obtaining a ‘present or absent’ answer for your sample then your result is secure. However, the test contains an Internal Extraction Control component that identifies if the sample is of high quality.
- Page 36 What do my results mean? “Test failed” Explanation The Positive Control is present to show that all aspects of the test are working correctly together. This error message shows that the quality control test has failed and the test as a whole is invalidated. This finding indicates that a problem has occurred in the reaction set-up part of the experiment and has nothing to do with sample preparation.
- Page 37 “Positive result lower than test sensitivity” Explanation The test is positive so if you are only interested in obtaining a ‘present or absent’ answer for your sample then your result is secure as a positive test. However, if the calculated percentage falls outside the accurate range for the test the exact percentage cannot accurately be calculated.
- Page 38 What do my results mean? “Test failed and is contaminated” Explanation The Positive Control is indicating test failure, and the Negative Control is indicating test contamination. Please read the “Test Failed” and “Test Contamination” sections of this technical support handbook for a further explanation.
- Page 39 Poor samples can result from overloading the DNA/ RNA extraction protocol with too much starting material. Try reducing the amount of starting material and repeat the sample preparation.
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Page 40: Troubleshooting
Troubleshooting Network connection problems: USB run fails to start:... - Page 41 Solution The software can connect to one or more instruments via an Ethernet network. There are two ways of configuring the network to allow this: Standard Network: The software and instruments will connect automatically to a standard network, where TCP/IP addresses are assigned automatically by a DHCP server.
- Page 42 Troubleshooting Unusual looking data: If your test results are not as you expect and you can see that the raw data looks unusual – poor quality amplification plots etc. Failed results: The instrument is reporting failed results.
- Page 43 Check that you have put all reaction tubes in the correct wells. If the positive and negative controls are in the wrong place this will cause problems. Please contact Primerdesign technical support for advice or manual data interpretation.
- Page 44 Please feel free to contact us for free advice or technical support. Primerdesign Ltd, York House, School Lane, Chandlers Ford, United Kingdom, SO53 4DG Telephone +44 (0)23 8074 8830 Fax +44 (0)870 836 2155 Orders: orders@primerdesign.co.uk...
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