Agilent Technologies 1290 Infinity II User Manual page 41

4
Preparing the Fraction Collector
Solvent Information
PEEK capillaries are very sensitive to high pressure. Therefore Agilent uses
stainless-steel cladded PEEK capillaries in bio-inert systems. The use of
stainless steel cladded PEEK capillaries keeps the flow path free of steel and
ensures pressure stability to at least 600 bar. If in doubt, consult the available
literature about the chemical compatibility of PEEK.
Polyimide
Agilent uses semi-crystalline polyimide for rotor seals in valves and needle
seats in autosamplers. One supplier of polyimide is DuPont, which brands
polyimide as Vespel, which is also used by Agilent.
Polyimide is stable in a pH range between 1 and 10 and in most organic
solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric
acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic
substances like ammonia (e.g. ammonium salts in basic conditions) or
acetates.
Polyethylene (PE)
Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow
piston and wash seals, which are used in 1290 Infinity pumps and for normal
phase applications in 1260 Infinity pumps.
Polyethylene has a good stability for most common inorganic solvents
including acids and bases in a pH range of 1 to 12.5. It is compatible to many
organic solvents used in chromatographic systems like methanol, acetonitrile
and isopropanol. It has limited stability with aliphatic, aromatic and
halogenated hydrocarbons, THF, phenol and derivatives, concentrated acids
and bases. For normal phase applications, the maximum pressure should be
limited to 200 bar.
Tantalum (Ta)
Tantalum is inert to most common HPLC solvents and almost all acids except
fluoric acid and acids with free sulfur trioxide. It can be corroded by strong
bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended
for the use with fluoric acid and fluorides.
1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 41