Focus On The Field Diaphragm And Center Its Position - Nikon ECLIPSE E200 MV Series Instruction Manual

9
Focus on the field diaphragm and
center its position.
(This adjustment is only for microscopes with
the field diaphragm.)
(1) Check that the 10x or 4x objective is in
the optical path. Turn the field
diaphragm ring to its right limit to stop
down the diaphragm.
(2) While looking into the eyepieces, rotate
the condenser focus knob so that the
outline of the field diaphragm image may
become clear.
When you rotate the condenser focus knob too
much to pass the focal point, the color of the image
outline looks reddish or bluish. Find the position
that the image outline has no color, where the
diaphragm image is in focus.
(3) Rotate the field diaphragm centering
screws until the field diaphragm image
comes to the center of the viewfield.
(4) Switch to the 40x objective. If the image
outline blurs, rotate the condenser focus
knob to obtain the best-focused position.
Note that the field diaphragm image focused with
the 40x objective is not so clear as that focused
with the 10x objective.
(5) Turn the field diaphragm ring so that the
field diaphragm image may become
about the same size as the viewfield.
(6) If the field diaphragm image is not
centered, use the centering screws
again.
If the field diaphragm image is not in the
exact center of the viewfield, it will be
off-centered especially when stopped down.
Perform the centering adjustment without
fail.
This completes the preparatory operations.
From the next step, you proceed to the actual microscopy for the target point of desired specimen
with the desired objective magnification.
Chapter 4 Microscopy Procedure (Detailed)
14
(1)
Turn the field
diaphragm ring
to stop down the
field diaphragm.
(2)
Rotate the condenser
focus knob to focus
on the field
diaphragm image.
Image of the
field diaphragm
(3)
Use the field diaphragm
centering screws to center
the image in the viewfield.
(4)(5)
Rotate the
condenser
focus knob to
focus on the
image.
Use the field
diaphragm ring to open
the image to the same
size as the viewfield.
(6)
Image of the field diaphragm
Use the field diaphragm
centering screws to center
the image in the viewfield.