GE EPS 3501 XL Operating Instructions Manual page 31

endpoint. The electrophoresis will be controlled by this voltage gradient provided the
limiting current or power is not attained. The EPS 3501 XL thus automatically switches
over the controlling parameter according to the programmed limits and conductivity
variations in the system.
E
3500
No.
A
B
C
Figure 4-3. Programming the voltage limiting profile in GRADIENT mode. The parameters shown
are the same as those listed in Table 4-2.
To illustrate voltage gradient programming, the programming for the IEF part of a 2-D
electrophoresis with Immobiline™ DryStrip Gels is shown in Table 4-2 and Figure 4-3.
Note that the first phase is a very steep gradient to reach the 500 V start level (0–500 V,
within 1 Vh). The next phase is actually a step since the endpoint for phase 2 is 500 V
which is the same as the endpoint for phase 1. Phase 3 is the "real" gradient, the voltage
is changed from 500 to 3500 V in 5 hours. The last phase is a step again, the endpoint
voltage is the same as for the phase before and the voltage will remain on 3500 V for
9.5 hours.
Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA
A
1 Vh
B
1
500
2500 Vh
Description
Phase 1
Phase 2
Phase 3
3
C
10000 Vh
2
5 10
No.
D
E
F
Operation 4
Setting up a run 4.3
D
33250 Vh
Description
Phase 4
Voltage (V)
Time (h)
4
19.5
F
31