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Related Manuals for THORLABS CM100
Summary of Contents for THORLABS CM100
- Page 1 CM100 Reflective Confocal Microscope User Guide...
- Page 3 Copyright © January 28, 2022 Thorlabs, Inc. All rights reserved. Information in this document is subject to change without notice. The software described in this document is furnished under a license agreement or nondisclosure agreement. The software may be used or copied only in accordance with the terms of those agreements.
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Page 5: Table Of Contents
3.2. Imaging Capabilities ..................... 3 Getting Started ........................4 Chapter 4 4.1. Unpacking and Inspection ................... 4 4.2. Setting up the CM100 ....................5 4.3. Cable Connection Diagram ..................8 4.3.1. Electronic Control Unit (ECU) Connections ................8 4.3.2. Galvo-Galvo Scanning Pair ....................10 Alignment .......................... -
Page 6: Chapter 1 Warning Symbol Definitions
CM100 Reflective Confocal Microscope Chapter 1: Warning Symbol Definitions Warning Symbol Definitions Chapter 1 Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current... -
Page 7: Chapter 2 Safety
Any modification or servicing of this system by unqualified personnel renders Thorlabs free of any liability. Only personnel authorized by Thorlabs and trained in the maintenance of this equipment should remove any covers or housings or attempt any repairs or adjustments. -
Page 8: Chapter 3 Description
The CM100 has a 35 mm x 52 mm x 3 mm silver-coated mirror at the front of the scan path that directs light from the scanners to the objective. In order to use this microscope for widefield as well as confocal imaging, you can replace the mirror with a beamsplitter. -
Page 9: Chapter 4 Getting Started
This section is provided for those interested in getting the Reflective Confocal Microscope up and running quickly. 4.1. Unpacking and Inspection Open the package, and carefully remove the CM100 and its accessories. Unpack the laser, Galvo-Galvo Controller, PMT, computer, and monitor from their respective packages. The table lists the standard accessories shipped with the device. -
Page 10: Setting Up The Cm100
This may cause poor quality images. A. Microscope 1. Use four screws (M6 for metric, 1/4-20 x 5/8" for imperial) to mount the CM100 onto the optical table. See the Mechanical Drawing Chapter on Page 25 for mounting holes. - Page 11 DO NOT switch on the laser while installing the Confocal system. This may cause injury due to laser radiation. Make sure the laser source is off. 1. Remove the red cap from the CM100 back panel. 2. Connect one end of the PM (Polarization-Maintaining) Patch cable to the laser and the other end to the CM100 back panel.
- Page 12 CM100 Reflective Confocal Microscope Chapter 4: Getting Started E. PMT 1. Use the post holder and clamping fork to mount the PMT onto the optical table. 2. Connect the USB port to the computer. 3. Connect the SMA to BNC cable from the OUT port of the PMT to the AI1 port on the NI Breakout Box.
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Page 13: Cable Connection Diagram
CM100 Reflective Confocal Microscope Chapter 4: Getting Started 4.3. Cable Connnection Diagram 4.3.1. Electronic Control Unit (ECU) Connections Figure 4-3.1 Electronic Control Unit (ECU), Rear View Page 8 TTN118785-D02... - Page 14 ECU IO | X IN: This is the command signal for the x galvanometer. It is not used in Thorlabs’ complete imaging systems setups. It may be monitored without interrupting the system, so long as the impedance is 1 kΩ.
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Page 15: Galvo-Galvo Scanning Pair
CM100 Reflective Confocal Microscope Chapter 4: Getting Started 4.3.2. Galvo-Galvo Scanning Pair With Galvo-Galvo systems there are two galvos, a fast (X axis) Galvo and a slow (Y axis) Galvo. The fast Galvo moves across the image linearly, with even velocity. It takes 200 µs to turnaround at the edges of the image. There is a turnaround at each side of the image. - Page 16 CM100 Reflective Confocal Microscope Chapter 4: Getting Started The slow Y Galvo moves once per image frame. During each sweep of the Y Galvo scanner, the ECU signals a frame trigger (FRAME TRIGGER OUT or vertical line sync) for acquisition synchronization.
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Page 17: Chapter 5 Alignment
CM100 Reflective Confocal Microscope Chapter 5: Alignment Alignment Chapter 5 You must align the microscope before imaging. There are two Alignment procedures: Illumination Alignment and Grid Slide Alignment. A. Illumination Alignment Perform the Illumination Alignment procedure prior to Grid Slide Alignment. - Page 18 CM100 Reflective Confocal Microscope Chapter 5: Alignment 4. Turn on all devices. For the laser, turn the POWER key switch clockwise. The unit is ON when the display lights up. DO NOT activate the laser at this step. 5. Use the PWR ADJUST knob on the laser to set it to maximum power.
- Page 19 Alignment tool. Use the iris on the Alignment tool to resize the beam on the center of the IR Disk. If the beam on the IR Disk appears clipped (not circular), please contact Thorlabs for assistance. Page 14...
- Page 20 CM100 Reflective Confocal Microscope Chapter 5: Alignment 10. Press and release the ENABLE switch to turn off the laser. 11. Remove the Alignment tool. 12. Place a piece of paper at the base of the microscope to visualize the beam.
- Page 21 CM100 Reflective Confocal Microscope Chapter 5: Alignment Beam that Requires Adjustment Adjusted Beam When you view the beam on the paper, the edges of the scanner mirrors give the beam a non-circular profile. The Illumination Alignment procedure is now complete.
- Page 22 CM100 Reflective Confocal Microscope Chapter 5: Alignment 4. In the Galvo/Galvo Area Control panel, set the parameters as shown in the image below. 5. Use the K-Cube Stepper Motor Controller to focus the objective onto the slide. 6. Use the 2 mm hex key to adjust the pinhole position in the X and Y directions to get maximum beam intensity (an XY Mount is attached to the pinhole inside the microscope).
- Page 23 CM100 Reflective Confocal Microscope Chapter 5: Alignment 7. In the Z Control panel, set the Slider Step Size to 1 µm. Click Set next to the Start field to store the Start location. 8. In the Z Control panel, use the ± button to gradually move the objective up (20 µm distance from the Start position).
- Page 24 CM100 Reflective Confocal Microscope Chapter 5: Alignment ▪ If there is only one maximum intensity for the image move the objective to the maximum point and adjust the pinhole’s X and Y position to maximize the intensity. Rev F, January 28, 2022...
- Page 25 CM100 Reflective Confocal Microscope Chapter 5: Alignment ▪ If there are two maximum intensities for the image, move the objective to the mid-point of the two maximum intensities. Then, adjust the pinhole’s X and Y position to maximize the intensity.
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Page 26: Widefield Imaging
Replace the silver-coated mirror at the front of the scan path with a user-supplied beamsplitter or appropriate dichroic filter to use the CM100 for widefield imaging, as well as confocal imaging. 1. Use the 2.5 mm hex key to remove the red front cover on the microscope. -
Page 27: Chapter 6 Maintaining The Cm100
Maintaining the CM100 Chapter 6 The unit does not need regular maintenance. If you suspect a problem with your CM100, please contact our nearest office for assistance from an application engineer (see the Thorlabs Worldwide Contacts Chapter on Page 27 for details). -
Page 28: Chapter 7 Specifications
CM100 Reflective Confocal Microscope Chapter 7: Specifications Specifications Chapter 7 Specification Value Excitation Laser Single Mode Fiber-Coupled Laser Wavelength 660 nm Max Output Power 15.0 mW (Min) Power Control Manual, 0 to 5 V External Modulation Signal Scanning Scan Head Galvo-Galvo Protected-Silver Coated with λ/4 Surface Flatness (P-V) - Page 29 This microscope includes an RMS20X objective that provides an effective magnification of 22.2X when used with the Confocal System as it is designed for a 180 mm tube lens and Thorlabs’ microscopes use a 200 mm tube lens focal length. See the Magnification & FOV tab for Cerna™-Based Confocal Systems on Thorlabs’...
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Page 30: Chapter 8 Mechanical Drawing
CM100 Reflective Confocal Microscope Chapter 8: Mechanical Drawing Mechanical Drawing Chapter 8 Rev F, January 28, 2022 Page 25... -
Page 31: Chapter 9 Regulatory
Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site. -
Page 32: Chapter 10 Thorlabs Worldwide Contacts
CM100 Reflective Confocal Microscope Chapter 10: Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to-date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. - Page 34 www.thorlabs.com...