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Related Manuals for THORLABS CM100
Summary of Contents for THORLABS CM100
- Page 1 Reflective Confocal Microscope CM100 User Guide...
- Page 2 The software may be used or copied only in accordance with the terms of those agreements. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or any means electronic or mechanical, including photocopying and recording for any purpose other than the purchaser's personal use without the written permission of ThorLabs, Inc.. ThorLabs, Inc.
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Page 3: Table Of Contents
Chapter 3 Description 3.1. Features at a Glance 3.2. Imaging Capabilities Chapter 4 Getting Started 4.1. Unpacking and Inspection 4.2. Setting up the CM100 4.3. Cable Connection Diagram Chapter 5 Alignment 5.1. Widefield Imaging Chapter 6 Maintaining the CM100 6.1. Cleaning 6.2. -
Page 4: Warning Symbols And Definitions
Chapter 1: CM100 Warning Symbols and Definitions Chapter 1 Warning Symbols and Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current... -
Page 5: Safety
WARNING DO NOT use the device for anything other than its intended use. If the device is used in a manner not specified by Thorlabs, the protection provided by the equipment may be impaired. WARRANTY WARNING DO NOT open the scanner block, electronics enclosure, detector module or computer. -
Page 6: Description
The CM100 has a 35 mm x 52 mm x 3 mm silver-coated mirror at the front of the scan path that directs light from the scanners to the objective. In order to use this microscope for widefield as well as confocal imaging, you can replace this mirror with a beamsplitter. -
Page 7: Getting Started
4.1. Unpacking and Inspection Open the package, and carefully remove the CM100 and its accessories. Unpack the laser, Galvo- Galvo Controller, PMT, computer, and monitor from their respective packages. The table lists the standard accessories shipped with the device. -
Page 8: Setting Up The Cm100
DO NOT place the SMA to BNC cable or DB-25 Connector cable near any electrical noise sources such as a power cable. This may cause poor quality images. A. Microscope Use four screws (M6 for metric/1/4-20x5/8" for imperial) to mount the CM100 onto the optical table. See the for mounting holes. - Page 9 DO NOT switch on the laser while installing the Confocal system. This may cause injury due to laser radiation. Make sure the laser source is off. Remove the red cap from the CM100 back panel. Connect one end of the PM (Polarization-Maintaining) Patch cable to the laser and the other end to the CM100 back panel.
- Page 10 Connect the SMA to BNC cable from the OUT port of the PMT to the AI1 port on the NI Breakout Box. Connect one end of the Armored Fiber Patch cable to the PMT and the other end to the Pinhole port in the CM100. Page 7 TTN118785-D02...
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Page 11: Cable Connection Diagram
Chapter 4: CM100 Getting Started 4.3. Cable Connection Diagram Laser CM100 Galvo/Galvo Controller PM Patch Cable DB-25 Connector Cable USB Cable NI SHC6868 Cable Computer Motorized Objective Arm Cable Armored Fiber Patch Cable NI SHC68-68-EPM Cable K-Cube K-Cube USB Cable... -
Page 12: Alignment
Chapter 5: CM100 Alignment Chapter 5 Alignment You must align the microscope before imaging. There are two Alignment procedures: Illumination Alignment and Grid Slide Alignment. A. Illumination Alignment Perform the Illumination Alignment procedure prior to Grid Slide Alignment. Use the 2.5 mm hex wrench to remove the red front and back cover of the microscope. - Page 13 Chapter 5: CM100 Alignment Turn on all devices. For the laser, turn the POWER key switch clockwise. The unit is ON when the display lights up. DO NOT activate the laser at this step. Use the PWR ADJUST knob on the laser to set it to maximum power.
- Page 14 Chapter 5: CM100 Alignment In the Galvo/Galvo Area Control panel, select Advanced and click WARNING DO NOT stare into the laser beam. Avoid direct eye exposure. Wear protective laser eyewear. Use proper laser safety procedures. Consult with your organization's laser safety officer for recommendations.
- Page 15 Use the iris on the Alignment tool to resize the beam on the center of the IR Disk. If the beam on the IR disk appears clipped (not circular), please contact Thorlabs for assistance. Press and release the ENABLE switch to turn off the laser.
- Page 16 Chapter 5: CM100 Alignment Beam that Requires Adjustment Adjusted Beam When you view the beam on the paper, the edges of the scanner mirrors give the beam a non-circular profile. The illumination alignment procedure is now complete. Page 13 TTN118785-D02...
- Page 17 Chapter 5: CM100 Alignment B. Grid Slide Alignment Make sure to perform the Illumination Alignment procedure prior to Grid Slide Alignment. Mount the objective onto the microscope. Use four screws and washers to mount the Rigid Stand Slide Holder on the optical table.
- Page 18 Chapter 5: CM100 Alignment Use the 2 mm hex key to adjust the pinhole position in the X and Y directions to get maximum beam intensity (a XY Mount is attached to the pinhole inside the microscope). In the Z Control panel, set the Slider Step Size to 1µm. Click Set next to the Start field to store the Start location.
- Page 19 Chapter 5: CM100 Alignment Follow one of the below procedures based on your observation. If there is only one maximum intensity for the image, move the objective to the maximum point and adjust the pinhole's X and Y position to maximize the intensity.
- Page 20 Chapter 5: CM100 Alignment If there are two maximum intensities for the image, move the objective to the mid point of the two maximum intensities. Then adjust the pinhole's X and Y position to maximize the intensity. After the adjustment, you can see only one maximum intensity for the image.
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Page 21: Widefield Imaging
Replace the silver-coated mirror at the front of the scan path with a user-supplied beam splitter or appropriate dichroic filter to use the CM100 for a widefield as well as confocal imaging. Use the 2.5 mm hex key to remove the red front cover on the microscope. -
Page 22: Maintaining The Cm100
CM100 Maintaining the CM100 Chapter 6 Maintaining the CM100 The unit does not need regular maintenance. If you suspect a problem with your CM100, please contact our nearest office for assistance from an application engineer (see Thorlabs Worldwide for details). -
Page 23: Specifications
Chapter 7: CM100 Specifications Chapter 7 Specifications Specification Value Excitation Laser Single Mode Fiber-Coupled Laser Wavelength 660 nm Max Output Power 15 mW (Min) Power Control Manual, 0 to 5 V External Modulation Signal Scanning Scan Head Galvo-Galvo Mirrors Protected-Silver Coated with λ/4 Surface Flatness (P-V) - Page 24 Chapter 7: CM100 Specifications Specification Value Coverslip Correction 0.17 mm Threading Fiber Patch Cables Laser to Scan Head 2 m PM Patch Cable, 620–850 nm, FC/PC Connectors MM Fiber, 1 m, Stainless Steel Protective Tubing, AR-Coated End Faces, Pinhole to Detector SMA Connectors...
- Page 25 This microscope includes an RMS20X objective that provides an effective magnification of 22.2X when used with the Confocal System as it is designed for a 180 mm tube lens and Thorlabs' microscopes use a 200 mm tube lens focal length. See Magnification & FOV tab for Cerna...
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Page 26: Mechanical Drawing
Chapter 8: CM100 Mechanical Drawing Chapter 8 Mechanical Drawing Microscope without Sample Holder Foot Landing for 2.36" 1.57" Accessories (4 Places) (60.0 mm) (40.0 mm) Imperial and Metric 3.15" Compatible Base Plate (80.0 mm) 6.88" (174.7 mm) 2.36" 5.20" 3.15"... -
Page 27: Regulatory
Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site. -
Page 28: Thorlabs Worldwide Contacts
Chapter 10: CM100 Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts For technical support or sales inquiries, please visit us at www.thorlabs.com/contact for our most up-to- date contact information. USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd.