THORLABS Cerna Series User Manual
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THORLABS Cerna Series User Manual

THORLABS Cerna Series User Manual

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Cerna® Mini Microscopes
User Guide

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Summary of Contents for THORLABS Cerna Series

  • Page 1 Cerna® Mini Microscopes User Guide...

  • Page 2: Table Of Contents

    Cerna Mini Microscopes Table of Contents Chapter 1 Warning Symbol Definitions ............1 Chapter 2 Safety ....................2 Chapter 3 Description ..................3 Features at a Glance .............. 3 Overview ................. 4 Chapter 4 Getting Started .................. 7 Ordering Codes ..............7 Unpacking and Inspection ............
  • Page 3 Cerna Mini Microscopes Chapter 8 Warranty ..................35 Chapter 9 Regulatory ..................36 Chapter 10 Thorlabs Worldwide Contacts ............. 37...

  • Page 4: Chapter 1 Warning Symbol Definitions

    Cerna Mini Microscopes Chapter 1: Warning Symbol Definitions Chapter 1 Warning Symbol Definitions Below is a list of warning symbols you may encounter in this manual or on your device. Symbol Description Direct Current Alternating Current Both Direct and Alternating Current Earth Ground Terminal Protective Conductor Terminal Frame or Chassis Terminal...

  • Page 5: Chapter 2 Safety

    WARNING DO NOT use the device for anything other than its intended use. If the device is used in a manner not specified by Thorlabs, the protection provided by the equipment may be impaired. CAUTION DO NOT operate in a wet, damp, or explosive environment.

  • Page 6: Chapter 3 Description

    Chapter 3 Description The Cerna Mini Microscopes provide simple microscope configurations that are ideal for experiments requiring simple imaging. Thorlabs offers four microscope configurations: SFM2 (Cerna Mini Microscope), SFM (Cerna Mini Microscope with Epi-Illuminator Module), SFMGFP (Cerna Mini Microscope with Epi-Illuminator Module for GFP/Alexa Fluor®...

  • Page 7: Overview

    Cerna Mini Microscopes Chapter 3: Description 10X Magnification Objective Filter Cube with Filter Set for GFP Epi-Illuminator Module for LED with 80° to 120° Divergence Angle Blue LED for GFP Excitation  470 nm Nominal Wavelength  710 mW Typical Output Power at Max Current ...
  • Page 8 Cerna Mini Microscopes Chapter 3: Description Camera Port Focus Module 3-Axis 3-Axis Controller Knob Box Figure 2 SFM2 Microscope Monochrome Gig-E Camera Camera Port Filter Cube Housing N10X-PF 3-Axis Knob Box 3-Axis SFM80 Controller Figure 3 SFMGFP Microscope T-Cube Focus LED Driver Module Rev.
  • Page 9 Cerna Mini Microscopes Chapter 3: Description sCMOS Camera Camera Port Filter Cube Housing N10X-PF 3-Axis Knob SFM80 3-Axis Controller Focus T-Cube Module LED Driver Figure 4 SFMGFP2 Microscope Page 6 TTN109666-D02...

  • Page 10: Chapter 4 Getting Started

    Cerna Mini Microscopes Chapter 4: Getting Started Chapter 4 Getting Started This section is provided for those interested in getting the Cerna Mini Microscopes up and running quickly. Ordering Codes Ordering Codes Description Cerna Mini Microscope with Epi-Illuminator Module SFM2 Cerna Mini Microscope Cerna Mini Microscope with Epi-Illuminator Module for SFMGFP...
  • Page 11 MCM3000 Series 3-Axis Controller User Guide Inspect the device and its accessories for any missing parts or damage. If there is any problem, please contact our nearest office (see Thorlabs Worldwide Contacts on page 37 for details). Page 8 TTN109666-D02...

  • Page 12: Cerna Mini Microscope Setup

    Cerna Mini Microscopes Chapter 4: Getting Started Cerna Mini Microscope Setup Note: To set up the SFM and SFM2 microscopes, follow the procedure in Section 4.3.1. For full functionality, the SFM will require the user to purchase an objective, light source, filter cube, fluorescence filter set, collimator and digital camera. The SFM2 will require an objective, light source, and digital camera.
  • Page 13 Cerna Mini Microscopes Chapter 4: Getting Started M4 x 12 mm Screw Figure 6 Mounting the Filter Cube Housing For SFM, use the 2 mm hex key to mount the Camera Port onto the Filter Cube Housing. For SFM2, mount the Camera Port directly to the Dovetail Plate.
  • Page 14 Cerna Mini Microscopes Chapter 4: Getting Started Connect the adapter cables from the Focus Module and PLS-XY Stage to the 3-Axis Knob Box. Connect the cable from the 3-Axis Knob Box to the 3-Axis Controller Box. Connect the power supply from the 3-Axis Controller Box to a standard power outlet.

  • Page 15: Fluorescence Filter Installation

    Install your filter set inside the filter cube. See the MDFM-MF2 manual for detailed instructions- https://www.thorlabs.com/manuals.cfm Slide the filter cube on the Filter Cube Housing cover and tighten the screw at the center of the cover...

  • Page 16: Led Power Source Installation

    Cerna Mini Microscopes Chapter 4: Getting Started 4.3.3 LED Power Source Installation For easy installation, the Filter Cube Housing included in the SFMGFP and SFMGFP2 configurations comes with a pre-installed LED and SFM80 collimation tube. Use two SHCS to mount the T-Cube LED Driver onto the optical table/breadboard.

  • Page 17: Camera

    Cerna Mini Microscopes Chapter 4: Getting Started 4.3.4 Camera Mount the camera (Monochrome Gig-E camera for SFMGFP or sCMOS camera for SFMGFP2) on the Camera Port. Refer to the included camera user guide for setup and installation. Figure 9 Mounting the Camera Page 14 TTN109666-D02...

  • Page 18: Chapter 5 Maintaining The Sfm Series

    Maintaining the SFM Series There are no serviceable parts in the SFM series modules. If you suspect a problem with your module, please contact our nearest office (see Thorlabs Worldwide Contacts on page 37 for details) for assistance from an applications engineer.

  • Page 19: Chapter 6 Specifications

    Cerna Mini Microscopes Chapter 6: Specifications Chapter 6 Specifications Microscope Body Specifications Specification Value Rail Length 174.3 mm (6.86") Post Length 200 mm (7.87") 162 mm (6.38”) Post Manual Height Adjustment Throat Depth 152.4 mm (6") Fine Motorized Motion X,Y, and Z Axes 1″...

  • Page 20: Motion Controller

    Cerna Mini Microscopes Chapter 6: Specifications Stepper Motor Specifications Thread Screw Pitch 0.3 mm Step Angle 1.8° Limit Switches Hall Effect Sensors Phase Current 0.49 A 5.1 Ω Phase Resistance Phase Inductance 1.5 mH 20 MΩ Insulation Resistance Motion Controller Specification Value Item #...

  • Page 21: Led Collimation Module Compatibility (Sfm/Sfmgfp/Sfmgfp2)

    Cerna Mini Microscopes Chapter 6: Specifications General Computer Connection USB 2.0 97.3 mm x 50.8 mm x 73.6 mm Housing Dimensions (W x D x H) (3.83″ x 2.00″ x 2.90″) LED Collimation Module Compatibility (SFM/SFMGFP/SFMGFP2) Collimation Module Item # Compatible LEDs SFM80 (Only Included with For Mounted LEDs with 80 - 120°...
  • Page 22 Cerna Mini Microscopes Chapter 6: Specifications 11 V (Min) LED Forward Voltage 12 V (Typical) Current Ripple 8 mA Current Ripple Frequency 570 kHz Modulation Mode Frequency Range 0 - 5 kHz, Sine Wave Modulation Form Arbitrary Input Voltage Rate 0 - 5 V Slew Rate 13.6 mA/µs...

  • Page 23: Fluorescence Filter Specifications (Sfmgfp/Sfmgfp2)

    Cerna Mini Microscopes Chapter 6: Specifications Fluorescence Filter Specifications (SFMGFP/SFMGFP2) Value Specification Excitation Emission Dichroic Filter Filter Filter Item # MDF-GFP Size Ø25 ± 0.1 mm Ø25 ± 0.1 mm 25.0 mm x 36.0 mm Clear Aperture >Ø21 mm >Ø21 mm >(22.5 mm x 32.4 mm) Angle of Incidence 0°...

  • Page 24: Monochrome Ccd Camera Specifications (Sfmgfp)

    Cerna Mini Microscopes Chapter 6: Specifications Figure 10 Objective Dimensional Drawing 6.10 Monochrome CCD Camera Specifications (SFMGFP) Specification Value Item # 1501M-GE Sony ICX285AL Monochrome CCD Sensor Type (Grade 0) Effective Number of Pixels (Horizontal x 1392 x 1040 Vertical) Imaging Area (Horizontal x Vertical) 8.98 mm x 6.71 mm Pixel Size...
  • Page 25 Cerna Mini Microscopes Chapter 6: Specifications Digital Output 14 Bit Cooling None Host PC Interface Gigabit Ethernet C-Mount (1.000″-32) Lens Mount Recommended System Requirements Operating System Windows 7, 8.1, or 10 (64 bit) ≥3.0 GHz Intel Core (i5 or Higher) Processor (CPU) ≥8 GB Memory (RAM)
  • Page 26 Cerna Mini Microscopes Chapter 6: Specifications Figure 11 Quantum Efficiency of 1501M-GE Camera Rev. D, July 9, 2018 Page 23...
  • Page 27 Cerna Mini Microscopes Chapter 6: Specifications Figure 12 IR-Blocking Filter Transmission of 1501M-GE Camera Page 24 TTN109666-D02...

  • Page 28: Scmos Camera Specifications (Sfmgfp2)

    Cerna Mini Microscopes Chapter 6: Specifications 6.11 sCMOS Camera Specifications (SFMGFP2) Specification Value Item # CM2100M-USB Sensor Type Monochrome sCMOS Effective Number of Pixels (Horizontal x 1920 x 1080 Vertical) Imaging Area (Horizontal x Vertical) 9.6768 mm x 5.4432 mm Pixel Size 5.04 µm x 5.04 µm Optical Format...
  • Page 29 Cerna Mini Microscopes Chapter 6: Specifications Adapter with ≥256 MB RAM Graphics Card Dedicated ≥600 W Power Supply Motherboard Integrated Intel USB 3.0 Controller USB or Internet Connectivity Connectivity for Driver Installation Data Rate Example Frame Rates at 1 ms Exposure Time 30 fps 50 fps...

  • Page 30: Chapter 7 Mechanical Drawings

    Cerna Mini Microscopes Chapter 7: Mechanical Drawings Chapter 7 Mechanical Drawings Figure 14 Mechanical Drawing of SFM Module (Side, Front, and Back View) Rev. D, July 9, 2018 Page 27...
  • Page 31 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 15 Mechanical Drawing of SFM Module (Top and Bottom View) Page 28 TTN109666-D02...
  • Page 32 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 16 Mechanical Drawing of SFM2 Module (Side, Front, and Back View) Rev. D, July 9, 2018 Page 29...
  • Page 33 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 17 Mechanical Drawing of SFM2 Module (Top and Bottom View) Page 30 TTN109666-D02...
  • Page 34 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 18 Mechanical Drawing of SFMGFP Module (Side, Front, and Back View) Rev. D, July 9, 2018 Page 31...
  • Page 35 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 19 Mechanical Drawing of SFMGFP Module (Top and Bottom View) Page 32 TTN109666-D02...
  • Page 36 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 20 Mechanical Drawing of SFMGFP2 Module (Side, Front, and Back View) Rev. D, July 9, 2018 Page 33...
  • Page 37 Cerna Mini Microscopes Chapter 7: Mechanical Drawings Figure 21 Mechanical Drawing of SFMGFP2 Module (Top and Bottom View) Page 34 TTN109666-D02...
  • Page 38 Chapter 8: Warranty Chapter 8 Warranty Thorlabs warrants that all products sold by Thorlabs will conform to the published specifications and shall be free from defects in material and workmanship under normal use, handling, and service. Thorlabs provides warranty for a period of one year.
  • Page 39 Waste Treatment is Your Own Responsibility If you do not return an “end of life” unit to Thorlabs, you must hand it to a company specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.
  • Page 40 Cerna Mini Microscopes Chapter 10: Thorlabs Worldwide Contacts Chapter 10 Thorlabs Worldwide Contacts USA, Canada, and South America UK and Ireland Thorlabs, Inc. Thorlabs Ltd. 56 Sparta Avenue 1 Saint Thomas Place, Ely Newton, NJ 07860 Cambridgeshire CB7 4EX Great Britain...
  • Page 41 www.thorlabs.com...

This manual is also suitable for:

Sfm2SfmSfmgfpSfmgfp2

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