Zeiss LSM 710 Manual

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Zeiss LSM 710

Startup

1. Turn on the Main Switch (see image below, #1).

2. Turn on the Systems/PC and Components Switches (see image below, #2).

3. Power up the X-Cite short arc lamp (see image below, #3).

Note: the lamp should not be turned on/off in quick succession. Always wait ca. 20 min.

4. Turn the key of the Argon laser power supply 90° (clockwise) from position 0 to 1 (see below, #4).

5. Turn on the PC (no image shown).

6. After about 5 min, switch the Lasos Argon laser control unit from 'idle' to 'run' (see image below,

#6). Wait until the green and/or red LED on the Lasos control unit lights up. If the red LED shows

up, turn the control knob counterclockwise until only the green LED is on.

Note: if you use only 488 nm at low power, you may leave it in 'idle' mode all the time.

7. Start the software: double-click on the ZEN icon. In the Login dialog, select 'Start System'. Once

ZEN is loaded, select the 'View' menu and check 'Show all (global)'.

1 LSM 710 – Quick Guide

MFPL BioOptics - Light Microscopy

Page 1 7. Start the software: double-click on the ZEN icon. In the Login dialog, select 'Start System'. Once ZEN is loaded, select the 'View' menu and check 'Show all (global)'. 1 LSM 710 – Quick Guide MFPL BioOptics - Light Microscopy...
Page 2 3. In ZEN, close your images. If you have used the 561 and/or 633 nm laser, leave them running. Leave ZEN running. 4. Disconnect from servers: HIVE: click on the HIVE icon. In the dialog popping up, select 'Disconnect' VBC share: click on the 'Logout' icon 2 LSM 710 – Quick Guide MFPL BioOptics - Light Microscopy...
Page 3: Troubleshooting
3. If there's a message "No connection to realtime controller", open the the door of the RTC (black box left to the microscope) , press the Reset Button (see image below, #3) and wait until ZEN resumes. 3 LSM 710 – Quick Guide MFPL BioOptics - Light Microscopy...
Page 4 INTERLOCK LED is off, exit ZEN, power off the power supply and turn it on again (ON/OFF switch beneath the thick cable. Do NOT use the Mains or Component Switches). 5. Contact the facility if the problem persists. 4 LSM 710 – Quick Guide MFPL BioOptics - Light Microscopy...