Zeiss LSM 710 Quick Manual
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Zeiss LSM 710 Quick Manual

Laser scanning microscopes
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  • Page 3: Table Of Contents

    Carl Zeiss Introduction This Quick Guide describes the basic operation of the LSM 710, LSM 780, LSM 710 NLO, LSM 780 NLO and ConfoCor 3 Laser Scanning microscopes with the ZEN 2012 software. The purpose of this document is to guide the user to get started with the system as quick as possible in order to obtain some first images from his samples.

  • Page 4: Starting The System

    L TUM=wbk= Carl Zeiss 1. Starting the System 1-1. Starting the System Switching on the LSM system 1-1-1. Switching on the X-Cite 120 or the HBO 100 mercury lamp 1-1-2. Switching on the Ar Laser 1-1-3. 1-1-4. Switching on the Ti:Sa Laser 1-1-1.
  • Page 5 1-2. Starting ZEN software Double click the ZEN icon on the desktop to start the Carl Zeiss LSM software. The ZEN Main Application window and the Startup window appear on the screen. Choose Start system to start the system hardware for acquiring new images.
  • Page 6 LSM710 / 780 ZEN Carl Zeiss 《 Introduction to ZEN – Efficient Navigation 》 The ZEN 2012 interface is clearly structured and follows the typical workflow of the experiments performed with confocal microscopy systems: Left Tool Area（Fig.3-①,②,③） The user finds the tools for sample observation, image acquisition, FCS, image processing and system maintenance, easily accessible via four Main Tabs.
  • Page 7 LSM710 / 780 ZEN Carl Zeiss Show all With Show all de-activated, the most commonly used tools are displayed. For each tool, the user can activate Show all mode to display and use additional functionality Fig.5 Show all More features of ZEN 2012 include: −...

  • Page 8: Turning On The Lasers

    LSM710 / 780 ZEN Carl Zeiss Work flow to acquire image 1. Starting the system, and Turning on the Lasers ↓ 2. Microscope Observation via Eye pieces ↓ 3. Configuring the LSM Beam path for used Dye ↓ 4. Scanning an Image 2.

  • Page 9: Setting Up The Microscope

    LSM710 / 780 ZEN Carl Zeiss 3. Setting up the microscope Changing between direct observation and laser scanning mode The Locate and Acquisition buttons switch between the use of the LSM and the microscope: • Click on the Locate tab for direct observation;...

  • Page 10: Configuring The Beam Path And Lasers

    LSM710 / 780 ZEN Carl Zeiss 4. Configuring the beam path and lasers Click on the Acquisition button to change to the LSM mode. There are 3 ways to configure the beam path and lasers. We highly recommend to use ‘4-1.Settings for track configuration’ especially for a user who is not familiar with LSM.
  • Page 11 LSM710 / 780 ZEN Carl Zeiss 4-2. Smart Setup ① Click on the Smart Setup button to open the smart setup window. ① ② Click on the arrow in the dye list and simply choose the dye(s) you use in your experiment from the list dialogue.
  • Page 12 LSM710 / 780 ZEN Carl Zeiss 4-3. Setting up a configuration manually ① Activate ‘Show all tools’. Fig.14 Show manual tools ② Open the Light Path tools to set-up the beam path. The Light Path tool displays the selected track configuration which is used for the scan procedure.
  • Page 13 LSM710 / 780 ZEN Carl Zeiss Activation / deactivation of the excitation wavelengths (check box) and setting of excitation intensities (slider) Selection of the main dichroic beam splitter (MBS) from the relevant list box. Selection of an emission range for scanning.

  • Page 14: Scanning An Image

    LSM710 / 780 ZEN Carl Zeiss 5. Scanning an image Workflow to acquire images Adjusting pinhole size ↓ Auto Exposure ↓ Live scan Adjusting Brightness/Contrast ↓ Setting the parameters ↓ Snap Adjusting pinhole size ① Select the Channels tool in the Left Tool Area. The Channels tool provides the control of the parameters for the individual detection channels.
  • Page 15 LSM710 / 780 ZEN Carl Zeiss Image acquisition – Auto Exposure Pressing the Auto Exposure button will then optimize the settings of the Gain (Master) and offset for the given laser power and pinhole size. Further image optimization from this point can be done easily.
  • Page 16 LSM710 / 780 ZEN Carl Zeiss Split View display the individual channels of a multi-channel image as well as the superimposed image. ※ The Dimensions View shows the Merged tick box to activate / deactivate the display of the channel overlay.
  • Page 17 LSM710 / 780 ZEN Carl Zeiss Image Optimization Choosing Range Indicator In the View – Dimensions View Option Control Block, click inside the color field in the button under the channel button (Fig. 22) Fig.21 Dimensions Control window The scanned image appears in a false-color...
  • Page 18 LSM710 / 780 ZEN Carl Zeiss Setting the parameters for scanning ⑥ Select the Acquisition Mode tool from the Left Tool Area. Fig.24 Acquisition Mode Frame Size Select the Frame Size as predefined number of pixels or enter your own values (default is 512 x 512) in the Acquisition Mode tool.
  • Page 19 LSM710 / 780 ZEN Carl Zeiss Averaging Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried out line-by-line or frame-by-frame. Frame averaging helps to reduce photo- bleaching, but does not give quite as smooth of an image.

  • Page 20: Storing And Exporting Image Data

    Merit) ・ ・ re-load your configuration from the stored image data. De-merit) ・ cannot load the image in other software. ※ You can download the free software ‘ZEN Light Edition’ from Carl Zeiss website. (Windows OS only) http://www.microimaging.zeiss.co.jp/ 2) Save as multipurpose format (Export), like TIF, JPEG…etc. (→page 20) Merit) ・...
  • Page 21 Fig.29 Save as window ③ Enter the file name and choose the appropriate image format. Note: the LSM 5 format is the native Carl Zeiss LSM image data format and contains all available extra information and hardware settings of your experiment.
  • Page 22 LSM710 / 780 ZEN Carl Zeiss 2) Export of Images as general formats ① Select the image to be exported, and c hoose File - Export from the menu. ② Select the file format, and the data type which the image is to be exported under Data.
  • Page 23 LSM710 / 780 ZEN Carl Zeiss 6-2. Open images To open images, select the file from File menu – Open or New File Browser. ZEN File Browser Advanced data browsing is available through the New File Browser (Ctrl + F or from the File Menu).

  • Page 24: Z Stack

    LSM710 / 780 ZEN Carl Zeiss 7. Z stack The Z-Stack function permits scanning a series of XY-images in different focus positions resulting in a Z-Stack, thus producing 3 dimensional data from your specimen. Scanning a Z stack ① Select Z-Stack in the main tools area.
  • Page 25 LSM710 / 780 ZEN Carl Zeiss ④ Click on the Smallest button to set number of slices to match the optimal Z-interval for the given stack size, objective lens, and the pinhole diameter. ⑤ Click on the Start Experiment button to start the recording of the Z-Stack.

  • Page 26: View Tabs

    LSM710 / 780 ZEN Carl Zeiss 8. View Tabs The View tabs make all viewing options and image analysis functions directly available from the main view. Switching from one View tab to another, changes the view type for the currently active image, keeping the image in the foreground.
  • Page 27 LSM710 / 780 ZEN Carl Zeiss 8-4. Ortho View ・ display a Z-Stack of images in an orthogonal view ・ measure distances in three dimensions Fig.41 Ortho View 8-5. Cut View ・ display a user defined section plane (= cut plane) of a Z- Stack.
  • Page 28 LSM710 / 780 ZEN Carl Zeiss 8-8. Co-localization View ・ permits interactive analysis of two channels of an image by computing a scatter diagram (co-localization) ・ Quantitative Colocalization Parameters shown in the Data Table ※ Tables can be saved by right-mouse Fig.45 Colocalization View...

  • Page 29: View Option Control Tab

    LSM710 / 780 ZEN Carl Zeiss 9. View Option Control tab (View Controller) These tabs allow individual activation / deactivation of the available View Option control blocks by clicking on the tabs. (The View tab Specific control tabs are marked with a blue triangle on their upper right corner.) ※...
  • Page 30 LSM710 / 780 ZEN Carl Zeiss 9-4. Overlay ・ add a scale bar to the image, as well as text annotations, ・ use a set of interactive measurement functions for length, angle, area and size, ① ② ③ ④ ⑤...
  • Page 31 LSM710 / 780 ZEN Carl Zeiss 9-5. Series This panel allows settings for the axis to be used for rotation of the 3D reconstructed images. ① Select 3D View ② Select the render mode, and set the position of the image (zoom, angle) in the Image Display window Fig.52 3D...

  • Page 32: Operation Of Microscope

    LSM710 / 780 ZEN Carl Zeiss 10. Operation of Light Microscope (Axio Observer. Z1) - 30 -...
  • Page 33 LSM710 / 780 ZEN Carl Zeiss TFT display touchscreen on the Axio Observer.Z1 On the motorized Axio Observer, the user can operate and configure the microscope and utilize optional functions using the TFT display. The TFT display is designed as a touch-sensitive screen.

  • Page 34: Switching Off The System

    11-4. Click on the File - Exit button to leave the ZEN software. 11-5. Shut down the computer. 11-6. Turn off the Power remote switch. ① Turn off the Components switch and the System/PC switch. ② Turn off the Main Switch. June. 2012 Carl Zeiss Microscopy Co., Ltd. - 32 -...

This manual is also suitable for:

Lsm 780Lsm 710 nloLsm 780 nloConfocor 3

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