Related Manuals for Zeiss LSM 5 MP
Summary of Contents for Zeiss LSM 5 MP
- Page 1 M i c r o s c o p y f r o m C a r l Z e i s s Quick Guide LSM 5 MP, LSM 510 and LSM 510 META Laser Scanning Microscopes LSM Software ZEN 2007 August 2007 We make it visible.
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Page 2: Table Of Contents
Contents Page Contents ..........................1 Introduction..........................1 Starting the System ....................... 2 Introduction to ZEN – Efficient Navigation ................5 Setting up the microscope....................10 Configuring the beam path and lasers ................12 Scanning an image....................... 15 Storing and exporting image data ..................20 Switching off the system..................... -
Page 3: Starting The System
Starting the System Switching on the LSM system " When set to ON the REMOTE CONTROL switch labeled System/PC provides power to the computer. This allows use of the computer and ZEN software offline (Fig. 1). " To completely switch on the system, now press the Components switch to ON. - Page 4 Starting the ZEN software " Double click the ZEN icon on the WINDOWS desktop to start the Carl Zeiss LSM software. The ZEN Main Application Window and the LSM 510 Startup window appear on the screen (Fig. 3) Fig. 3 ZEN Main Application Window at Startup (a) and the LSM 510 Startup Window (b and c) In the small startup window, choose either to start the system online ("Start System"...
- Page 5 Fig. 4 ZEN Main Application Window after Startup with empty image container Fig. 5 ZEN Main Application Window after Startup with several images loaded 08/2007...
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Page 6: Introduction To Zen - Efficient Navigation
Introduction to ZEN – Efficient Navigation ZEN - Efficient Navigation - is the new software for the LSM 5 Family from Carl Zeiss. With the launch of this software in 2007 Carl Zeiss sets new standards in application-friendly software for Laser Scanning Microscopy. - Page 7 The Pro-Basic concept ensures that tool panels are never more complex than needed. In Basic Mode, the most commonly used tools are displayed. For each tool, the user can activate Pro Mode to display and use additional functionality (Fig. 6). Fig.
- Page 8 The ZEN software will ask you if you want to save your unsaved images when you try to close the application. Note: There is no "image database" any more like in the earlier Zeiss LSM software versions. Fig. 8 New image document in the Open Images Ares...
- Page 9 Advanced data browsing is available through the File Browser (Ctrl-F or from the File Menu). The File Browser can be used like the WINDOWS program file browser. Images can be opened by a double-click and image acquisition parameters are displayed with the thumbnails (Fig. 9). For more information on data browsing please refer to the detailed operating manual.
- Page 10 (Fig. 10). Remember, the argon multi-line laser has to first be put to standby for a 5 minute warm-up before it changes to on. " Zeiss recommends operating the Argon multi-line Laser at a tube current of about 6 A (#50 % output). This is the best compromise between laser stability/power and laser life-time. (tube-current control can be found in Pro Mode).
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Page 11: Setting Up The Microscope
Setting up the microscope Changing between direct observation, camera detection and laser scanning mode The Ocular, Camera and LSM buttons switch the beam path and indicate which beam path is currently in use for the microscope: " Click on the Ocular button to change the microscope beam path for direct observation via the eyepieces of the binocular tube, lasers are blocked. - Page 12 Setting the microscope for reflected light " Click on the Reflected Light icon to open the X-Cite 120 Controls and turn it on. " Click on the Reflected Light shutter to open the shutter of the X-Cite 120 lamp / HBO100. "...
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Page 13: Configuring The Beam Path And Lasers
Configuring the beam path and lasers " Click on the LSM button. Choosing a configuration Simultaneous scanning of single, double and triple labeling: Advantage: faster image acquisition Disadvantage: Eventual cross-talk between channels Sequential scanning of double and triple labeling; line-by-line or frame-by-frame: Advantage: Only one detector and one laser are switched on at any one time. - Page 14 Settings for track configuration in Channel Mode " Select Channel Mode if necessary (Fig. 15). " Click on the LSM tab (Fig. 14). The Light Path tool displays the selected track configuration which is used for the scan procedure. " You can change the settings of this panel using the following function elements: Fig.
- Page 15 " For storing a new configuration enter a desired name first line Configurations list box (Fig. 17) and click Store. " For loading an existing configuration select it from the list box and click on the Load button. " For deleting an existing configuration select it in Fig.
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Page 16: Scanning An Image
Scanning an image Setting the parameters for scanning " Select the Acquisition Mode tool from the Left Tool Area (Fig. 18). " Select the Frame Size as predefined number of pixels or enter your own values (e.g. 300 x 600) in the Acquisition Mode tool. - Page 17 Setting scan averaging Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried out line-by-line or frame-by-frame. Frame averaging helps to reduce photo-bleaching, but does not give quite as smooth of an image. " For averaging, select the Line or Frame mode in the Acquisition Mode tool. "...
- Page 18 Image acquisition Once you have set up your parameter as defined in the above section, you can acquire a frame image of your specimen. " Use one of the Find, Fast, Continuous, or Single buttons to start the scanning procedure to acquire an image.
- Page 19 The scanned image appears in a false-color presentation (Fig. 22). If the image is too bright, it appears red on the screen. Red = saturation (maximum). If the image is not bright enough, it appears blue on the screen. Blue = zero (minimum). Adjusting the laser intensity "...
- Page 20 Scanning a Z stack " Open the Z Stack tool in the Left Tool Area. " Select Mode First/Last on the top of the Z Stack tool. " Click on the button in the Action Button area. A continuous XY-scan of the set focus position will be performed.
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Page 21: Storing And Exporting Image Data
" The WINDOWS Save As window appears. " Enter a file name and choose the appropriate image format. Note: the LSM 5 format is the native Carl Zeiss LSM image data format and contains all available extra information and hardware settings of your experiment. -
Page 22: Switching Off The System
Switching off the system " Click on the File button in the Main Menu bar and then click on the Exit button to leave the ZEN software. " If any lasers are still running you should shut them off now in the pop-up window indicating the lasers still in use.