Nikon N-STORM User Manual

The Nikon N-STORM User Guide

General Guidelines:

If you get stuck at any point, do not understand something or find the system does not
function properly ALWAYS warn the staff!!!
Microscope:
1. NEVER switch the microscope and stage off as the system as the heat generated by of the
motors, circuits and perfect focus system can generate excessive drift while heating up.
2. Do not forget to switch the 405 nm laser of as its switch is not as visible as for the others.
Objectives:
1. Objectives are very expensive always handle with extreme delicacy especially while changing
samples. Never touch or scratch the front lens directly.
2. When starting to work check for visible damage.
3. Always start imaging with the 10x objective and proceed to larger magnifications. This helps
to avoid crashing into the sample and damaging the objective.
4. When changing objectives always make sure the distance between the objective and stage is
appropriate. ESPECIALLY if using a different stage insert than the previous user.
5. When applying oil always check if the objective is indeed an oil immersion objective.
6. After using the 100x oil immersion objective NEVER forget to clean with lens cleaning paper:
1. Fold the lens cleaning paper three times to form a strip, 2. Hold it on the two ends on both
sides, 3. Gently wipe with left-right motion. Never press with your finger directly. 4. Repeat
previous step until you cannot see oil stain and repeat once after that one final time.
7. When finished ALWAYS turn to the 10x objective and lower the stage to a safe distance.
Sample Holder:
1. When using the universal sample holder (Ti-SH-U) never loosen the left slider!! This is the
fixed point and changing its position will prohibit users to find positions when reimaging slides.
Lasers:
1. Only switch on lasers that will be used for your experiments.
Computers:
1. Never save data on the local hard drives. Please use the network drives instead.
Booking: