Determining Copper Concentration In Samples - Hach APA 6000 Operation Manual

Testing Samples
1.
Using a volumetric
pipet, transfer 5 mL of
APA Copper Reagent 2
(indicator) into a 100-mL
volumetric flask. Add
approximately 75 mL
of sample to the flask
and mix.

5.3 Determining Copper Concentration in Samples

6202918bench.fm
2.
Add 5 mL of APA
Copper Reagent 1 (buffer)
to the same flask. Fill to
the mark with sample.
Invert the flask at least
seven times to mix.
Blank Absorbance of 0 ppm standard
=
Slope = Abs. of 1 ppm standard – Blank absorbance

Sample Abs. Blank Abs.
------------------------------------------------------------------- -
Copper (ppm) =

440 nm
3.
Within 10 minutes of
adding reagents, transfer
3 mL of sample into a 1-cm
cell. Measure the
absorbance at 440 nm.

–

Slope
Determining Copper Concentration in Samples
Section 5
4.
Calculate the total
copper concentration in
the sample using the
following equations.
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