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Original method
0
5
Figure 2-7 Automatic Run Extension Examples
Figure 2-7 illustrates the possible run extensions. The original
method was programmed to rise to the maximum %B over
seven minutes, hold for two minutes and return to the
minimum %B for a final minute. At nine minutes, a peak was
eluting. The system extended the run, holding the %B at the
maximum level for another five minutes. This also occurred at
fourteen and nineteen minutes, resulting in the second and
third extensions. At 24 minutes, the %B solvent strength
returned to zero. Before the final minute elapsed, more
compound was detected, causing the system to extend the run
for a final five minutes at the minimum %B.
2.11.6 Set Default Tube Volumes
Click this button to open a window from which you can set the
default volume of the collection tubes. You can increase or
decrease these values to set the desired default volume
collected in each container. Just be sure you don't overflow the
containers by exceeding their capacity.
The system will automatically advance to the next tube when
this default volume is met in each tube. Keep in mind that
advanced method settings (discussed in Section 3 and the
on-line help) may override this default setting. During a run,
other factors such as automatic tube advances for detected
peaks will affect collected volume.
2.11.7 Set Date/Time
Click this button to open a window from which you can set the
system date and time.
Extension 2
Extension 1
10
15
Minutes
Section 2 System Preparation
Final Extension
Extension 3
20
25
2-15
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