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DR/2400
Method 10050
Scope and Application:
* This test is semi-quantitative. Results are expressed as greater or less than the threshold value used.
Tips and Techniques
• Read the entire procedure before starting. Identify and have ready all the necessary reagents, cuvettes, and other apparatus
before beginning the analysis. A 1-cm square cell holder is required.
• Timing is critical; follow instructions carefully.
• A consistent technique when mixing the cuvettes is critical to this test. The best results come from using the cuvette rack and
mixing as described in Using the 1-cm MicroCuvette Rack on page 7. Cuvettes can be mixed individually, but test results may not
be as consistent.
• Handle the cuvettes carefully. Scratches on the inside or outside may cause erroneous results. Carefully clean the outside of the
cuvettes with a clean absorbent cloth or tissue before placing them into the instrument.
• Antibody cuvettes and enzyme conjugate are made in matched lots. Do not mix reagent lots.
• Twenty Antibody Cuvettes are provided with each reagent set. One Antibody Cuvette will be used for each calibrator or sample.
Antibody Cuvettes are not reusable.
• To avoid damaging the Color Developing Solution, do not expose it to direct sunlight.
• There are two protocols in this procedure, one for levels of 1 ppm and 5 ppm, and another for 10 ppm and 50 ppm. Each uses a
different quantity of calibrator and sample extract as follows:
• To test across ranges, such as 1 and 50 ppm, test the lower concentration first. If the result is positive then test at the higher level.
If the result of the test at the lower concentration is negative, the higher range test will be negative also, and need not be
performed.
• The same filtered extract can be used for both protocols if it is tightly capped between assays. The maximum time between
assays cannot exceed one-half hour.
• Store the reagents at 4 °C when they are not in use. Allow the reagents to reach room temperature before using them in an
analysis. Actual testing may be done at temperatures ranging from 1– 38 °C.
• The Soil Extractant contains methyl alcohol which is poisonous and flammable. Before using this and other reagents, read the
Material Safety Data Sheet (MSDS) for proper use of protective equipment and other safety information.
• Hach Company recommends wearing protective nitrile gloves for this procedure.
PCB_None_Other_IMM_Eng_Ody.fm
For soil
This method analyzes for PCB that has been extracted from soil samples. Sample
extracts, calibrators, and reagents are added to cuvettes coated with PCB-specific
antibodies. The color that develops is then measured and compared with the
color measurements of the calibrators. The test requires about 20 minutes for
complete analysis. As many as 10 cuvettes can be run simultaneously.
Range
(as Arochlor 1248)
1 ppm and 5 ppm
10 ppm and 50 ppm
PCB (Polychlorinated
Immunoassay Method*
Volume of calibrator and
sample extract used
50 µL
10 µL
PCB (Polychlorinated Biphenyls)
Biphenyls)
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