Absorbance And Transmittance Measurement Procedure - Hach 2100AN IS User Manual

Absorbance and transmittance measurement procedure

Note: To measure samples with negative absorbance, set the analytical zero using the sample with the greatest absorbance, and measure the sample with the least
absorbance. Report the reading as negative absorbance.
1. Push UNITS/Exit 2. Using the manual
until "%T" or "ABS" is flow cell kit, install the
shown on the display. flow cell. Refer to
a flow cell
on page 31.
Note: The sample cell
cover does not close
when the flow cell is
installed.
28 English
3. Slowly put 250 mL of
100 %T or zero
Using absorbance reference
solution down the
interior edge of the inlet
reservoir.
Put the sample down
the interior edge of the
reservoir to prevent air
bubbles in the sample.
4. Push CAL/Zero. 5. Push ENTER.
The display shows "100 The instrument display
%T" or zero. counts down from 30 to
0.
Note:
The instrument starts Note: If the value
analytical zeroing for shown is not 100 %T,
transmittance, and 0.000 A, or if dashes
absorbance modes at flash, do steps 5 and 6
the same time. again.
6. Slowly put 250 mL of
the sample in the inlet
reservoir.