ThermoFisher Scientific Invitrogen NEON1 Manuals

Manuals and User Guides for ThermoFisher Scientific Invitrogen NEON1. We have 1 ThermoFisher Scientific Invitrogen NEON1 manual available for free PDF download: User Manual

ThermoFisher Scientific Invitrogen NEON1 User Manual (90 pages)

NxT Electroporation System For Electroporation of Mammalian Cells, Including Primary and Stem Cells, with High Transfection Efficiency

Brand: ThermoFisher Scientific | Category: Laboratory Equipment | Size: 11 MB

Table of Contents
3
CHAPTER 1 Product Information

6
- Product Description
  6
- System Overview
  6
- Features
  7
- Product Contents
  7
- Neon ™ Nxt System Contents
  7
- Neon ™ Nxt Kit Contents
  8
- System Components
  9
- Neon ™ Nxt Device
  9
- Neon ™ Nxt Pipette Station
  10
- Neon ™ Nxt Pipette
  11
- Neon ™ Nxt Kit
  11
- Neon ™ Nxt Tip
  11
- Neon ™ Nxt Tube
  12
- Upon Receiving the Device
  12
- Unpacking Instructions
  12
- Getting Started
  13
- Set up the Neon ™ Nxt Electroporation System
  13
- User Interface Overview
  16
- Touchscreen Controls
  16
- Connect the Instrument to the Internet
  19
- Create a User Profile on the Instrument
  23
- Manage User Profiles
  23
- About the Thermo Fisher ™ Connect Platform
  25
- Features
  25
- Create a Connect Platform Account
  25
- Create a PIN Number
  25
- Set up a New Administrator
  26
- Add an Instrument to Your Connect Platform Account
  26
CHAPTER 2 Methods

28
- Electroporation Protocol Options
  28
- Electroporation Parameters
  28
- Create or Edit Protocols
  29
- Create a Protocol
  29
- Edit a Protocol
  30
- Save a Protocol
  30
- Create or Edit Plate Maps
  31
- Create a Plate Map
  31
- Edit a Plate Map
  32
- Save a Plate Map
  33
- Manage Protocols
  33
- Import or Export Protocols
  33
- General Guidelines
  37
- Recommended Kits
  37
- Recommended Buffers
  38
- DNA Quality and Amount
  38
- Sirna Quality and Amount
  38
- Controls
  38
- Using the Neon ™ Nxt Electroporation System
  39
- Required Materials Not Supplied
  39
- Calculate Electroporation Volumes for Cell Suspension
  40
- Prepare Adherent Cells
  40
- Prepare Suspension Cells
  42
- Set up the Pipette Station
  43
- Load the Pipette
  43
- Run Electroporation Protocol
  45
- Maintenance
  47
- Cleaning and Maintenance
  47
- Replace the Fuses
  48
- Upgrade the System Firmware
  48
- Self Verification Test
  50
- Restore Factory Settings (Administrator Profile Only)
  51
- Repackaging the Instrument
  51
APPENDIX A Troubleshooting

53
- Instrument Error Codes
  55
APPENDIX B Optimization

60
- Optimization Protocol
  60
APPENDIX C Recommended Instrument Settings

61
- About Instrument
  61
- Recommended Instrument Settings
  61
- Instrument Settings (Administrator Only)
  62
- Maintenance & Services
  65
- Run History
  66
APPENDIX D Specifications

68
- Product Specifications
  68
- Networking Requirements
  69
APPENDIX E Related Products

70
- Accessory Products
  70
- Additional Products
  70
APPENDIX F Safety

78
- Safety Information
  78
- Informational Symbols
  78
- Informations de Sécurité
  80
- Informational Symbols
  80
- Environmental Requirements
  82
- Safety and Electromagnetic Compatibility (EMC) Standards
  83
- Safety
  83
- Emc
  83
- Class a Notice
  83
- Environmental Design Standards
  84
- Electrical Safety
  84
- Service Operation Requirements
  84
- Chemical Safety
  86
- Biological Hazard Safety
  87
APPENDIX G Documentation and Support

88
- Customer and Technical Support
  88
- Limited Product Warranty
  88

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Laboratory Equipment Measuring Instruments Microscope Data Loggers Security Sensors

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ThermoFisher Scientific Invitrogen NEON1 Manuals

ThermoFisher Scientific Invitrogen NEON1 User Manual (90 pages)

Table of Contents

CHAPTER 1 Product Information

CHAPTER 2 Methods

APPENDIX A Troubleshooting

APPENDIX B Optimization

APPENDIX C Recommended Instrument Settings

APPENDIX D Specifications

APPENDIX E Related Products

APPENDIX F Safety

APPENDIX G Documentation and Support

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ThermoFisher Scientific Categories