ThermoFisher Scientific Invitrogen iBlot 3 Manuals

Manuals and User Guides for ThermoFisher Scientific Invitrogen iBlot 3. We have 1 ThermoFisher Scientific Invitrogen iBlot 3 manual available for free PDF download: User Manual

ThermoFisher Scientific Invitrogen iBlot 3 User Manual (54 pages)

Western Blot Transfer System For dry electroblotting of proteins from mini and midi gels

Brand: ThermoFisher Scientific | Category: Laboratory Equipment | Size: 3 MB

Table of Contents
3
CHAPTER 1 Product Information

6
- Product Contents
  6
- Iblot ™ 3 Western Blot Transfer Device Contents
  6
- Before Starting
  6
- Installing the Instrument
  7
- Service Operation Requirements
  8
- Unpacking Instructions
  8
- Upon Receiving the Instrument
  8
- Unpacking the Iblot ™ 3 Western Blot Transfer Device
  8
- 3 Western Blot Transfer Device
  8
- Device Views
  8
- About the System
  9
- Iblot ™ 3 Western Blot Transfer System
  9
- Features
  9
- System Components
  9
- System Overview
  9
- Description of Parts
  11
- Iblot ™ 3 Western Blot Transfer Device
  11
- Blotting Surface
  11
- Lids and Cooling
  12
- Control Panel
  12
- Blotting Roller
  12
- Power Cord and Power Adapters
  12
- Transfer Stacks
  12
- Guidelines for Iblot ™ 3 Transfer Stacks
  13
- Top Stack
  14
- Bottom Stack
  15
- Transfer Membrane
  16
- Iblot ™ Filter Paper
  17
- Absorbent Pad
  17
- Operating the Iblot
  18
- First Time Usage of the Iblot ™ 3 Western Blot Transfer Device
  18
- Control Panel of the Iblot ™ 3 Gel Transfer Device
  18
- Set the Date and Time
  19
- Write Logs to a USB Storage Device
  19
- Description of Methods
  20
- Methods
  20
- Recommended Running Parameters
  20
CHAPTER 2 Protein Transfer Protocol

22
- Experimental Overview
  22
- Experimental Outline
  22
- General Guidelines
  22
- Recommended Gel Types
  23
- Using the Iblot
  23
- Materials Needed
  23
- Select a Method
  24
- Assemble the Iblot ™ 3 Transfer Stack
  24
- Perform Blotting
  26
- Disassemble the Iblot ™ 3 Transfer Stack
  26
CHAPTER 3 Custom Methods

28
APPENDIX A Troubleshooting

32
- Introduction
  32
APPENDIX B Post Transfer Analysis

35
- Post Transfer Analysis
  35
APPENDIX C Optimizing Blotting

36
- Optimizing Blotting
  36
APPENDIX D Maintenance

37
- Cleaning
  37
- General Maintenance
  37
- Replacing the Fuse
  37
- Replacing Electrical Contacts
  39
APPENDIX E Product Specifications

41
- Iblot ™ 3 Western Blot Transfer Device Specifications
  41
- Iblot ™ 3 Transfer Stack Specifications
  42
APPENDIX F Accessory Products

43
- Transfer Stacks
  43
- Additional Products
  43
- Precast Gels and Premade Buffers
  44
APPENDIX G Safety

45
APPENDIX H Documentation and Support

52
- Customer and Technical Support
  52
- Limited Product Warranty
  52

ThermoFisher Scientific Categories

Laboratory Equipment Measuring Instruments Microscope Data Loggers Security Sensors

More ThermoFisher Scientific Manuals

ThermoFisher Scientific Invitrogen iBlot 3 Manuals

ThermoFisher Scientific Invitrogen iBlot 3 User Manual (54 pages)

Table of Contents

CHAPTER 1 Product Information

CHAPTER 2 Protein Transfer Protocol

CHAPTER 3 Custom Methods

APPENDIX A Troubleshooting

APPENDIX B Post Transfer Analysis

APPENDIX C Optimizing Blotting

APPENDIX D Maintenance

APPENDIX E Product Specifications

APPENDIX F Accessory Products

APPENDIX G Safety

APPENDIX H Documentation and Support

Related Products

ThermoFisher Scientific Categories