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Tenmars TM-740 User Manual

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Accela Pump
Hardware Manual
60157-97000 Revision D
February 2009

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  • Page 1 Accela Pump Hardware Manual 60157-97000 Revision D February 2009...
  • Page 2 © 2009 Thermo Fisher Scientific Inc. All rights reserved. Accela, ChromQuest, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Super Flangeless is a trademark of Upchurch Scientific. PEEK is a trademark of Victrex PLC. The following are registered trademarks in the United States and other countries: Windows is a registered trademark of Microsoft Corporation.
  • Page 3 Regulatory Compliance Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in the next section by product name. Changes that you make to your system might void compliance with one or more of these EMC and safety standards.
  • Page 4 CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device. Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.

  • Page 5: Weee Compliance

    WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 6 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 7 CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA Electric Shock: This instrument uses Elektroschock: In diesem Gerät werden Choc électrique: L’instrument utilise des Descarga eléctrica: Este instrumento Shock da folgorazione. L’apparecchio è high voltages that can cause personal Hochspannungen verwendet, die tensions capables d’infliger des blessures utiliza altas tensiones, capaces de alimentato da corrente ad alta tensione...
  • Page 8 CAUTION Symbol CAUTION Electric Shock: This instrument uses high voltages that can cause personal injury. Before servicing, shut down the instrument and disconnect the instrument from line power. Keep the top cover on while operating the instrument. Do not remove protective covers from PCBs. Chemical: This instrument might contain hazardous chemicals.

  • Page 9: Table Of Contents

    Contents Preface .............xiii Related Documentation .
  • Page 10 Contents Chapter 4 Preparing the Pump for Operation ........37 Removing Air from the Solvent Lines .
  • Page 11 Contents Baseline Noise at Low Pressures........88 Insufficient Solvent Flow from the Pump Outlet.

  • Page 13: Preface

    Preface This Accela Pump Hardware Manual provides you with information on how to install, maintain, and troubleshoot the Accela Pump. You operate the Accela Pump from a computer running Xcalibur™ (version 2.0 or higher) or ChromQuest (version 4.2 or higher). Related Documentation In addition to this guide, Thermo Fisher Scientific provides the following documents for the Accela Pump:...

  • Page 14: Special Precautions For The Accela Pump

    Preface Special Precautions for the Accela Pump Note the caution sticker on the front panel of the Accela Pump shown below. The drive arm mechanism that controls the movement of the pistons contains moving parts that can pinch your fingers. White, insulated, electrical wires connect the actuating coils at the ends of the proportioning valves to the pump as shown below.

  • Page 15: Good Laboratory Practices

    Preface Good Laboratory Practices To obtain optimal performance from your LC system and to prevent personal injury or injury to the environment, do the following: • Keep good records. • Read the manufacturers’ Material Safety Data Sheets (MSDSs) for the chemicals being used in your laboratory.
  • Page 16 Preface Solvent Requirement Many chemical manufacturers provide a line of high-purity or HPLC-grade reagents that are free of chemical impurities. Routine filtration of all solvents or eluents through a 0.45 or 0.2 micron (or less) fluorocarbon filter before placing them in the solvent reservoir significantly prolongs the life and effectiveness of the inlet filters, check valves and seals, injector, and column.

  • Page 17: Contacting Us

    Preface Contacting Us There are several ways to contact Thermo Fisher Scientific for the information you need. To contact Technical Support Phone 800-532-4752 561-688-8736 E-mail us.techsupport.analyze@thermofisher.com Knowledge base www.thermokb.com Find software updates and utilities to download at mssupport.thermo.com. To contact Customer Service for ordering information Phone 800-532-4752 561-688-8731...

  • Page 19: Introduction

    Introduction The Accela Pump is a member of the Accela™ family of ultra high performance liquid chromatography instruments. This chapter provides an introduction to the Accela Pump shown in Figure Contents • Functional Description • Specifications Figure 1. Accela Pump Thermo Scientific Accela Pump Hardware Manual...

  • Page 20: Functional Description

    Introduction Functional Description Functional Description The Accela Pump is a quaternary, low-pressure mixing pump with built-in solvent degassing and pulse dampening systems. The pumping system provides optimum performance in the flow rate ranges needed for liquid chromatography and mass spectrometry. You can run precise gradients from 50 to 1000 μL/min.

  • Page 21: Vacuum Degasser Assembly

    Introduction Functional Description The following topics describe the main features of the Accela Pump: • Vacuum Degasser Assembly • Liquid Displacement Assembly (LDA) • Purge Valve • Pulse Dampening Assembly • Dynamic Mixer • Status LEDs Vacuum Degasser Assembly The Accela Pump contains a built-in vacuum membrane degasser that removes dissolved gasses from the mobile phase.

  • Page 22: Purge Valve

    Introduction Functional Description pumps. In addition, the ruby ball and sapphire seat are virtually wear-free as opposed to the polymeric membrane of a diaphragm valve, which becomes more brittle and less responsive as it ages. Purge Valve The purge valve is located to the left of the LDA (see Figure 3).

  • Page 23: Pulse Dampening Assembly

    Introduction Functional Description Pulse Dampening Assembly The pulse dampening assembly is located behind the front panel of the pump to the left of the LDA (see Figure Figure 4. Pulse dampening assembly (located behind the front panel to the left of the LDA) Purge valve Pressure sensor...

  • Page 24: Dynamic Mixer

    Introduction Functional Description CAUTION Keep the pulse dampener flush valve closed during normal operation of the pump. If you do accidentally open the valve while you are pumping an acidic or buffered mobile phase, prime the pulse dampener with an organic solvent such as IPA or methanol as soon as you notice the error.

  • Page 25: Status Leds

    Introduction Functional Description Status LEDs Four status LEDs—labeled Power, Communication, Run, and Degas—are located on the left door of the pump (see Figure 1 page 1 Figure Table 1 describes these LEDs. Figure 5. Front panel LEDs Power Communication Degas Table 1.

  • Page 26: Specifications

    Introduction Specifications Specifications The specifications for the pump are as follows. 1.0 μL/min isocratic, 50 μL/min gradient Minimum flow rate: 1000 μL/min Maximum flow rate: 0.1 μL/min Flow rate resolution: Pressure range: 0 to 1000 bar (0 to 14500 psi) Operating mode: •...

  • Page 27: Installation

    Installation This chapter provides instructions for the initial installation of your Accela Pump, including connections to other modules in the system. Use the Installation Checklist on the back of this page as a quick reference. Make a copy of the checklist and fill it out when you have successfully completed the installation.

  • Page 28: Installation Checklist

    Installation Installation Checklist Installation Checklist For the proper installation of your Accela Pump, complete these steps and keep this checklist in your maintenance records. Unpack and inspect your instrument (see “Unpacking and Inspecting the Instrument” page 11). Read the safety precautions. Position the pump appropriately (see “Making the Initial Instrument Preparations”...

  • Page 29: Unpacking And Inspecting The Instrument

    Installation Unpacking and Inspecting the Instrument Unpacking and Inspecting the Instrument Carefully remove the pump from the shipping container and inspect both the pump and packing for any signs of damage. If you find any damage, save the shipping materials and immediately contact the shipping company.

  • Page 30: Making The Initial Instrument Preparations

    Installation Making the Initial Instrument Preparations Making the Initial Instrument Preparations Place the pump on a laboratory bench as close as possible to a designated electrical outlet. Be sure to place the system in a draft-free location away from an open window, air conditioner vents, or other circulating air source.

  • Page 31: Making The Initial Back Panel Connections

    Installation Making the Initial Back Panel Connections Making the Initial Back Panel Connections Before you can operate the Accela Pump, connect the power cable and the USB communication cable. To operate the Accela Pump as part of the LC system, connect the interconnect cable that is used to coordinate the operation of the pump with the other Accela modules.

  • Page 32: Connecting The Power Cable

    Installation Making the Initial Back Panel Connections Connecting the Power Cable The pump is shipped with a power cord appropriate for the shipping destination. CAUTION Never connect the pump to, or operate the pump with, an electrical line source that has power drops or fluctuations of 10% below the nominal rated line voltage. To connect the pump to line power 1.

  • Page 33: Connecting The System Interconnect Cable

    Installation Making the Initial Back Panel Connections Connecting the System Interconnect Cable The system interconnect cable synchronizes the timing of the Accela devices. The interconnect cable has seven combicon connectors: three are labeled DETECTOR; two are labeled PUMP; one has a small, A/S tag on its adjacent cable; and one has a small, M/S tag on its adjacent cable (see Figure Figure 9...
  • Page 34 Installation Making the Initial Back Panel Connections Figure 9. Schematic of the connections for the system interconnect cable Accela PDA Detector System interconnect cable Accela Autosampler Accela Pump RS232 1 2 3 4 5 6 7 8 Accela Pump Accela Pump Hardware Manual Thermo Scientific...

  • Page 35: Connecting The Low-Pressure Solvent Lines

    Installation Connecting the Low-Pressure Solvent Lines Connecting the Low-Pressure Solvent Lines Four low-pressure solvent lines connect the solvent bottles in the solvent platform to the pump. Connect these solvent lines before you operate the pump. Because the Accela Pump contains very small, precision-machined parts that can be affected by small particles, solvents should always be HPLC-quality and free of particulate matter.
  • Page 36 Installation Connecting the Low-Pressure Solvent Lines 2. Place the label A sticker onto one of the four solvent reservoir bottle caps. 3. Pass one end of the inlet line for solvent A through the solvent reservoir bottle cap labeled A. Terminate the inlet line with one of the solvent reservoir filters included in the system accessory kit (see Figure 10).

  • Page 37: Installing The Super Flangeless Fittings

    Installation Connecting the Low-Pressure Solvent Lines Installing the Super Flangeless Fittings The Super Flangeless™ fitting consists of three components: a nut, a ferrule, and a stainless steel compression ring (see Figure 11). The compression ring has two sides (see Figure 12).
  • Page 38 Installation Connecting the Low-Pressure Solvent Lines To install a Super Flangeless fitting 1. Slide the nut onto the end of the tubing. 2. With its flattened side facing toward the nut, slide the compression ring onto the end of the tubing (see Figure 13).
  • Page 39 Installation Connecting the Low-Pressure Solvent Lines d. Unscrew the swaged fitting and verify the following (see Figure 15): • The end of the square-cut tubing is flush with the end of the ferrule. • The steel compression ring is seated over the ferrule. Figure 15.

  • Page 40: Connecting The Pump To The Autosampler

    Installation Connecting the Pump to the Autosampler Connecting the Pump to the Autosampler The Accela System Kit contains 1/16 in. OD × 0.005 in. ID stainless steel tubing precut to 20 cm and high-pressure fittings. These items are used to connect the outlet of the Accela Pump to the heat exchanger of the Accela Autosampler.
  • Page 41 Installation Connecting the Pump to the Autosampler Figure 18. Connection between the pump and the autosampler Connection to inlet of heat LDA outlet exchanger 10 cm length of high-pressure tubing 20 cm length of high-pressure tubing Mixer outlet PU RGE Inline filter holder PULSE DAMPENER...

  • Page 42: Powering On For The First Time

    Installation Powering On for the First Time Powering On for the First Time After you install the solvent lines, you can turn on the pump. To turn on the pump 1. Ensure that the power switch on the front of the pump below the left door is in the Off position (released or out position).

  • Page 43: Data System Instrument Configuration

    Data System Instrument Configuration To add the Accela Pump to the data system instrument configuration and to ensure that the Accela Autosampler recognizes the input signals issued by the pump, follow the procedures in this chapter. Contents • Adding the Pump to the Xcalibur Instrument Configuration •...
  • Page 44 Data System Instrument Configuration Adding the Pump to the Xcalibur Instrument Configuration Figure 19. Instrument Configuration window (for Xcalibur versions lower than 2.1) Accela Pump Hardware Manual Thermo Scientific...
  • Page 45 Data System Instrument Configuration Adding the Pump to the Xcalibur Instrument Configuration Figure 20. Thermo Foundation Instrument Configuration window (Xcalibur 2.1 or higher) 2. To show all the instruments, in the Device Types list, select All. The installed instruments appear as buttons in the Available Devices pane. 3.

  • Page 46: Configuring The Signal Polarity For The Accela As In Xcalibur

    Data System Instrument Configuration Configuring the Signal Polarity for the Accela AS in Xcalibur Figure 21. Accela Pump dialog box (Xcalibur) 5. Click OK to close the Accela Pump dialog box. 6. Click Done to save the configuration and close the Instrument Configuration window. Configuring the Signal Polarity for the Accela AS in Xcalibur During an injection sequence, the pump issues a Pump Ready signal and an Injection Hold Release signal to the autosampler.
  • Page 47 Data System Instrument Configuration Configuring the Signal Polarity for the Accela AS in Xcalibur Note In Xcalibur, the instrument setup method for the pump contains a custom stability limit parameter. This parameter allows the user to define the acceptable level of pressure stability that must be attained before the pump issues the Pump Ready signal to the autosampler.

  • Page 48: Configuring An Accela Instrument In Chromquest

    Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest Figure 22. Configuring the input signal polarities for the Accela Autosampler (Xcalibur) Configuring an Accela Instrument in ChromQuest An Accela LC system consists of an analytical pump, an autosampler, and a PDA detector. To configure an Accela instrument in ChromQuest 1.
  • Page 49 Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest The Instrument Configuration dialog box appears (see Figure 23). Figure 23. Instrument Configuration dialog box (ChromQuest) 4. Do the following: a. In the Instrument name box, type a name that identifies your instrument. b.
  • Page 50 Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest Figure 25. Accela dialog box 5. In the Available modules pane, do the following: a. Double-click the Accela Pump button. A copy of the button appears in the Configured modules pane. b.
  • Page 51 Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest 6. Configure the Accela Pump options: a. In the Configured modules pane, double-click the Accela Pump button. The Accela Pump Configuration dialog box appears (see Figure 27). Figure 27. Accela Pump Configuration dialog box (ChromQuest) Note In ChromQuest 5.0, the Accela Pump Configuration dialog box includes a Handle Degasser Feedback check box.
  • Page 52 Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest Figure 28. Accela AS Configuration dialog box 8. Configure the Accela PDA Detector: a. Double-click the Analog button in the Configured modules pane. The Detector Configuration dialog box appears. b. In the Detector name box, type an identifying name for the PDA detector. c.
  • Page 53 Data System Instrument Configuration Configuring an Accela Instrument in ChromQuest e. Keep the stack value at its default of 01 unless you are controlling more than one PDA detector from the data system computer. Note The stack value must match the unit ID setting on the back panel of the PDA detector.

  • Page 55: Preparing The Pump For Operation

    Preparing the Pump for Operation After you connect the pump to the data system computer, connect the solvent lines to their respective degas inlets, and configure the pump to communicate with the data system, you are ready to prepare the pump for operation. Before you operate the Accela Pump for the first time, remove air from the solvent lines and prime the pulse dampener.

  • Page 56: Using The Data System To Start And Stop The Solvent Flow

    Preparing the Pump for Operation Using the Data System to Start and Stop the Solvent Flow Figure 29. Using a syringe to draw solvent through a solvent reservoir line PURGE PULSE D A MPENER F L USH Low-pressure solvent line disconnected from the A outlet port of the built-in degassing unit 3.

  • Page 57: Using Xcalibur To Start And Stop The Solvent Flow

    Preparing the Pump for Operation Using the Data System to Start and Stop the Solvent Flow Using Xcalibur to Start and Stop the Solvent Flow You can start and stop the solvent flow by using the Direct Control dialog box in the Xcalibur data system.

  • Page 58: Using Chromquest To Start And Stop The Solvent Flow

    Preparing the Pump for Operation Using the Data System to Start and Stop the Solvent Flow 9. Click (Stop) to stop the solvent flow. 10. If you opened the purge valve to let the solvent flow bypass the LC column, close the purge valve by turning the wingnut clockwise.

  • Page 59: Priming The Pulse Dampener

    Preparing the Pump for Operation Priming the Pulse Dampener 6. If you do not want the LC column in the solvent path, do the following: a. Open the purge valve by turning the wingnut counterclockwise. b. Place a beaker under the purge valve to collect solvent. 7.

  • Page 61: Diagnostics

    Diagnostics Use the following procedures to monitor the pump pulsation, test the pump proportioning accuracy, and determine the delay volume of the pump. Contents • Monitoring the Pump Pulsation • Testing the Pump Proportioning • Determining the Delay Volume of the Pump Monitoring the Pump Pulsation The pump continuously monitors the system pressure and bases its pressure readout on a 30 s interval.

  • Page 62: Using Xcalibur To Monitor The Pump Status

    Diagnostics Monitoring the Pump Pulsation Using Xcalibur to Monitor the Pump Status You can find information about the pressure variation (absolute pressure and standard deviation of the pressure) on the Status page of the Information view. This view appears on the left side of the Home Page window.

  • Page 63: Using Chromquest To Monitor The Pump Status

    Diagnostics Monitoring the Pump Pulsation Using ChromQuest to Monitor the Pump Status Table 3 lists the possible statuses for the Accela Pump. Table 3. Status descriptions for the Accela Pump Status Meaning Ready to Download The pump is online, but is producing a zero flow rate. Homing The pistons are moving to the home position.

  • Page 64: Testing The Pump Proportioning

    Diagnostics Testing the Pump Proportioning Figure 33. Pump Status area of the Accela Pump page (ChromQuest) Pump status System backpressure and pressure standard deviation 3. Check the status of the Accela Pump. 4. Check the system backpressure and the pressure SD (based on pressure readings taken over a 30 second period).
  • Page 65 Diagnostics Testing the Pump Proportioning Use the following detector settings and chromatographic conditions to run and monitor the step gradient listed in Table 200 μL/min • Flow: • Pressure: approximately 100 bar by restriction • Eluents: A: methanol B: spiked methanol •...
  • Page 66 Diagnostics Testing the Pump Proportioning Figure 34. Step gradient profile for testing compositional accuracy Step 5 100% Step 4 Step 3 Step 2 Step 1 Time (min) Step heights should be within ±1% of the theoretical values. In the step gradient shown in Figure 34, the absorbance value for 100% spiked solvent is 600 mAU (600 mAU –...

  • Page 67: Determining The Delay Volume Of The Pump

    Diagnostics Determining the Delay Volume of the Pump Determining the Delay Volume of the Pump The gradient delay volume of your pump is an important variable if you are developing gradient methods that you plan to transfer to other instruments. The gradient delay volume of the Accela Pump consists of the wetted components that lie in the flow path between the solvent proportioning valves of the pump and the outlet of the LDA.
  • Page 68 Diagnostics Determining the Delay Volume of the Pump 4. From the Xcalibur data system, do the following: a. In the Direct Control dialog box for the Accela Pump, select the Take the pump under control check box (see “Using Xcalibur to Start and Stop the Solvent Flow” page 39).
  • Page 69 Diagnostics Determining the Delay Volume of the Pump Figure 35. Graphical display of the baseline shift that occurs as the spiked mobile phase reaches the detector Solvent proportioning valve to detector Baseline shift Inject Time (min) Thermo Scientific Accela Pump Hardware Manual...

  • Page 71: Maintenance

    Maintenance To properly maintain your pump, monitor its performance. If the pressure pulsation increases or the proportioning accuracy decreases, take remedial action. To lengthen the lifespan of the seals, always flush the piston guide bushings with distilled water after pumping buffered solutions.

  • Page 72: Flushing The Piston Guide Bushings

    Maintenance Flushing the Piston Guide Bushings Flushing the Piston Guide Bushings As the pistons move back and forth within the displacements chambers, a small quantity of mobile phase leaks behind the primary piston seals. With buffered mobile phases, a precipitate (also known as creep or salt build-up) forms as the liquid fraction evaporates.

  • Page 73: Minimizing Pump Pulsation

    Maintenance Minimizing Pump Pulsation Minimizing Pump Pulsation Trapped air bubbles, contaminated proportioning valves, or contaminated check valves can cause excessive pump pulsation. If the pulsation of the pump is greater than 1 percent at pressures greater than 100 bar, purge air out of the piston chambers, and clean the proportioning valves and the check valves.

  • Page 74: Cleaning The Lda Components Without Disassembling The Lda

    Maintenance Minimizing Pump Pulsation Figure 37. View of the tubing connected to the purge valve Tubing (with fitting PURGE for syringe) PULSE DAMPENER FLUSH Cleaning the LDA Components without Disassembling the LDA Contaminated check valves and contaminated proportioning valves can cause an excessive variation in backpressure.
  • Page 75 Maintenance Minimizing Pump Pulsation 4. If you were pumping buffered mobile phase through the system, pump HPLC-grade water on all four channels at a flow rate of 200 μL/min for 30 minutes. Empty the contents of the syringe into a suitable waste receptacle, and then pump an additional 6 mL of water through the LDA.

  • Page 76: Cleaning The Lda Components After Disassembling The Lda

    Maintenance Cleaning the LDA Components after Disassembling the LDA Cleaning the LDA Components after Disassembling the LDA The check valves, pistons, displacements chambers, and inlet module are the most likely components to have problems with contamination. You cannot clean the primary piston seals. If the primary piston seals contain particulate matter, replace them.

  • Page 77: Cleaning The Displacement Chambers And The Inlet Module

    Maintenance Cleaning the LDA Components after Disassembling the LDA Cleaning the Displacement Chambers and the Inlet Module Clean the displacement chambers if the primary piston seals have started to decompose and you notice black, fibrous, particles in the chamber. To clean the displacement chambers and the inlet module 1.

  • Page 78: Removing The Lda From The Pump

    Maintenance Removing the LDA from the Pump Removing the LDA from the Pump To remove the LDA from the front panel of the pump, use the following tools. Almost all of these tools are provided in the Accela Pump Accessory Kit: •...
  • Page 79 Maintenance Removing the LDA from the Pump Figure 39. View of the high-pressure tubing connections Crossover Connection to tubing outlet module PU RGE PULSE DAMPENER FLUSH Captive screws (3x) 5. Unscrew the low-pressure solvent lines connected to the solvent proportioning valves. 6.
  • Page 80 Maintenance Removing the LDA from the Pump 7. Using a 2.5 mm balldriver, remove the two M3 screws that secure the drive arm cover to the pump (see Figure 41). Figure 41. Removing the drive arm cover Drive arm cover M3 screws 8.
  • Page 81 Maintenance Removing the LDA from the Pump 10. Gently push the piston heads to the left so that they do not contact the Z-arms as shown Figure Figure 44. View of the piston heads pushed to the left Piston heads 11.

  • Page 82: Disassembling The Lda

    Maintenance Disassembling the LDA Disassembling the LDA To prevent contamination of the internal surfaces of the LDA, make sure that you have a clean benchtop to work on before you disassemble the LDA. To replace the piston seals, remove the holding hooks, bushings, and pistons from the LDA body.
  • Page 83 Maintenance Disassembling the LDA 2. Remove the holding hooks, bushings, and pistons from the LDA body (see Figure 47). Figure 47. Removing the holding hooks, bushings, and pistons from the LDA body Guide bushing Piston Holding hook If you are replacing the primary piston seals but do not need to clean the stacked components of the LDA, go to “Replacing the Primary Pistons Seals”...

  • Page 84: Removing The Stacked Components From The Lda Body

    Maintenance Disassembling the LDA Removing the Stacked Components from the LDA Body To replace the check valves, remove the stacked components from the LDA body. To remove the stacked components from the LDA body 1. Using the 4 mm L-Hex wrench, loosen the top set screw, and then slide the LDA compression lid out (see Figure 48).

  • Page 85: Replacing The Primary Pistons Seals

    Maintenance Replacing the Primary Pistons Seals Figure 49. Removing the stacked components from the LDA body Upper displacement chamber Outlet Lower displacement chamber module and check valves Replacing the Primary Pistons Seals When you disassemble the LDA, you must replace the primary piston seals. To replace the primary piston seals 1.
  • Page 86 Maintenance Replacing the Primary Pistons Seals Figure 50. View of the piston inserted into the holding hook and the secondary piston seal installed on the piston Secondary piston seal Piston Holding hook 6. Slide the guide bushing onto the piston (see Figure 51).
  • Page 87 Maintenance Replacing the Primary Pistons Seals Figure 52. View of the final assembly Guide bushing Primary piston seal 6 mm 8. Finish reassembling the LDA: • If you did not remove the stacked components from the LDA, go to “Installing the Holding Hooks and the Piston Assemblies”...

  • Page 88: Assembling The Liquid Displacement Assembly

    Maintenance Assembling the Liquid Displacement Assembly Assembling the Liquid Displacement Assembly To assemble the LDA, follow these procedures: Connecting the Quaternary Inlet Module to the LDA Body Inserting the Check Valves into the Lower Displacement Chamber Inserting the Components of the LDA Stack into the LDA Body Installing the Holding Hooks and the Piston Assemblies Connecting the Quaternary Inlet Module to the LDA Body To connect the quaternary inlet module to the LDA body...

  • Page 89: Inserting The Check Valves Into The Lower Displacement Chamber

    Maintenance Assembling the Liquid Displacement Assembly Inserting the Check Valves into the Lower Displacement Chamber Note There are two versions of the LDA. For information on the discontinued version, Appendix A, “Discontinued Versions of the Accela Pump.” To install the check valves 1.
  • Page 90 Maintenance Assembling the Liquid Displacement Assembly Figure 56. Installing the stacked components of the LDA LDA body PEEK sealing rings Outlet module Upper displacement chamber Lower displacement chamber with check valves 4. Slide the compression lid into the upper bracket of the LDA body (see Figure 57).

  • Page 91: Installing The Holding Hooks And The Piston Assemblies

    Maintenance Assembling the Liquid Displacement Assembly 5. Using a 4 mm L-hex wrench, tighten the compression lid as shown in Figure Figure 58. Tightening the compression lid Installing the Holding Hooks and the Piston Assemblies Note For instructions on assembling the holding hook and piston assembly, see “Replacing the Primary Pistons Seals”...
  • Page 92 Maintenance Assembling the Liquid Displacement Assembly 2. Slide the pistons as far as possible into the displacement chambers (see Figure 60). The piston acts as a guiding aid as you tighten the fastening screws in step Figure 60. View of the piston pushed into the displacement chamber 3.

  • Page 93: Connecting The Lda To The Pump

    Maintenance Connecting the LDA to the Pump Figure 62. View of the LDA with the wash tubing installed on the guide bushings Wash tubing Connecting the LDA to the Pump After you reassemble the LDA, connect it to the front panel of the pump. To connect the LDA to the front panel of the pump 1.
  • Page 94 Maintenance Connecting the LDA to the Pump Figure 63. View of the alignment pins for the LDA body Alignment pins 2. Alternately tighten the three captive screws that secure the LDA to the pump. 3. Push the heads of the pistons toward the arms of the drive mechanism, leaving a gap between the piston head and the brackets at the ends of the arms (see Figure 64).

  • Page 95: Servicing The Proportioning Valves

    Maintenance Servicing the Proportioning Valves 5. Using a 4 mm L-hex wrench, tighten the clamping screws on the right side of the drive arm mechanisms. 6. Using a 2.5 mm balldriver, replace the drive arm cover and tighten the screws. 7.
  • Page 96 Maintenance Servicing the Proportioning Valves Occasionally, after extended periods of disuse, the free-floating piston inside the proportioning valve freezes against its sapphire seat. When this occurs, dislodging the frozen valve requires a greater mechanical force than the magnetic field applied by the actuating coils. You can dislodge the frozen proportioning valve with the proportioning valve tool supplied in the accessory kit.

  • Page 97: Replacing The Solvent Reservoir Filters

    Maintenance Replacing the Solvent Reservoir Filters Replacing the Solvent Reservoir Filters The solvent reservoir filter removes particulate matter from the mobile phase before it enters the solvent line. Eventually particulate matter from the mobile phase builds up on the filter and restricts the flow of solvent.

  • Page 98: Replacing A Fuse

    Maintenance Replacing a Fuse Replacing a Fuse Instrument power is supplied by two 2.0 amp, 250 V, TL, 5 mm OD × 20 mm length fuses. The fuses are housed in the fuse compartment on the lower right corner as you are facing the back panel of the pump (next to the power cord).

  • Page 99: Troubleshooting

    Troubleshooting If properly maintained, the Accela Pump can operate free of trouble for many years. You can avoid most pump problems with periodic maintenance, as described in Chapter 6, “Maintenance.” However, in the event that an error message is displayed or you suspect a mechanical or electrical failure, you might be able to diagnose the source of the problem.

  • Page 100: General System Troubleshooting

    Troubleshooting General System Troubleshooting General System Troubleshooting The following are common problems that occur with a liquid chromatography system. No flow 1. Check mobile phase connections. 2. Check for leaks. 3. See problem 8 on page High backpressure 1. Check the flow rate and system/column specifications. 2.
  • Page 101 Troubleshooting General System Troubleshooting Poor peak shape 1. Check the system for leaks. 2. Check the fittings and tubing lengths. 3. Check the column performance. 4. Refer to the troubleshooting guide for your autosampler. 5. Refer to the troubleshooting guide for your detector. Poor retention time reproducibility 1.

  • Page 102: Pump Troubleshooting Guide

    Troubleshooting Pump Troubleshooting Guide Pump Troubleshooting Guide The information in Table 7 can help you to identify specific pump-related problems. Table 7. Troubleshooting pump-related problems (Sheet 1 of 2) Problem Cause Diagnostic/Remedy 1. No response a. Loose power cord a. Plug the power cord into line power when power is b.
  • Page 103 Troubleshooting Pump Troubleshooting Guide Table 7. Troubleshooting pump-related problems (Sheet 2 of 2) Problem Cause Diagnostic/Remedy 6. Proportioning a. Loose actuating coil a. Reattach the actuating coil (see page valves do not b. Defective proportioning valve b. Contact your Thermo Fisher Scientific service click representative c.
  • Page 104 Troubleshooting Pump Troubleshooting Guide To reconnect the loose LED cable 1. Using a #2 Phillips screwdriver, remove the screw that secures the LED cover to the inner-left door, and then remove the cover. Screw 2. Reconnect the LED cable to the board. LED cable 3.

  • Page 105: Pump Pulsation

    Troubleshooting Pump Pulsation Pump Pulsation Pump pulsation is the variation in pressure with time due to the motion of the pump pistons. Pump pulsation can be divided into these categories: • Normal Pressure Pulsation • Abnormal Pressure Pulsation Normal Pressure Pulsation The pump continuously monitors the system pressure and bases its pressure readout on a 30 second interval.

  • Page 106: Baseline Noise At Low Pressures

    Troubleshooting Baseline Noise at Low Pressures If the problem still persists after replacing the seals, the problem is likely due to a contaminated check valve. For low flow (< 50 μL/min) applications, cleaning a check valve is normally not effective. Most likely you must replace one or both check valve assemblies. Pressure Pulsations with SD between 3 and 10 Bar Pressure pulsations with a standard deviation of 3 to 10 bar are of various origins.

  • Page 107: Insufficient Solvent Flow From The Pump Outlet

    Troubleshooting Insufficient Solvent Flow from the Pump Outlet Insufficient Solvent Flow from the Pump Outlet If there is insufficient flow when the pump is running, the problem is usually a result of one of the following: • Pump Cavitation • Blocked Proportioning Valve •...

  • Page 108: Blocked Check Valve

    Troubleshooting Irreproducible Retention Times Occasionally, a valve might freeze closed if it hasn’t been used for an extended period of time. To open the valve, insert the valve tool (stainless steel wire) into the valve inlet and push the floating piston away from the valve seat, as described in “Servicing the Proportioning Valves”...

  • Page 109: Chapter 8 Accessories And Replacement Parts

    Accessories and Replacement Parts This chapter contains a list of replaceable parts and their corresponding part numbers. To place an order, contact your local Thermo Fisher Scientific service representative. Contents • Liquid Displacement Assembly (LDA) Parts • Accela Pump Accessory Kit •...

  • Page 110: Liquid Displacement Assembly (Lda) Parts

    Accessories and Replacement Parts Liquid Displacement Assembly (LDA) Parts Liquid Displacement Assembly (LDA) Parts The part number for the liquid displacement assembly (LDA) is 00950-01-00111 The LDA replaceable parts listed in Table 8 correspond to the items labeled in Figure Figure 70, and Figure...
  • Page 111 Accessories and Replacement Parts Liquid Displacement Assembly (LDA) Parts Figure 69. Liquid displacement assembly (original version, parts 1 to 8) (1) Outlet module (2) PEEK film seal (3) Upper displacement chamber (4) Primary piston seal (Fluoroloy 907) (2) PEEK film seal (5) Outlet holder and check valve (6) Lower displacement chamber (4) Primary piston seal...
  • Page 112 Accessories and Replacement Parts Liquid Displacement Assembly (LDA) Parts Figure 70. Liquid displacement assembly (current version, parts 1 to 8) (1) Outlet module (2) PEEK film seal (3) Upper displacement chamber (4) Primary piston seal (Fluoroloy 907) (2) PEEK film seal (5) Check valve and PEEK film seals (6) Lower displacement chamber (4) Primary piston seal (Fluoroloy 907)
  • Page 113 Accessories and Replacement Parts Liquid Displacement Assembly (LDA) Parts Figure 71. Liquid displacement assembly (parts 9 to 17) (9) Compression lid LDA body (10) Guide bushing Holding hook (11) Quaternary inlet module (12) Piston (sapphire) (14) Secondary piston seal (15) Actuating coil (13) Nylon set screw (17) Proportioning valve (16) Proportioning valve seal ring...
  • Page 114 Accessories and Replacement Parts Liquid Displacement Assembly (LDA) Parts Table 9. Additional LDA parts Item Description Part number Material Pressure sensor and pulse dampener unit 00950-01-00112 Ti, 316 stainless steel Wing screw for purge valve 00950-01-00117 Purge valve spindle 00950-01-00118 Ti/Ruby Crossover tubing 00950-01-00113...

  • Page 115: Accela Pump Accessory Kit

    Accessories and Replacement Parts Accela Pump Accessory Kit Accela Pump Accessory Kit The following items are contained in the Accela Pump Accessory Kit (P/N 60157-62001). Cable, assembly, RS232, shielded, external PC ......72011-63008 Cable, USB, 3 m length, ROHS .

  • Page 116: Solvent Interconnect Kit

    Accessories and Replacement Parts Solvent Interconnect Kit Solvent Interconnect Kit The following items are included in the Solvent Interconnect Kit (P/N F5050-010). Bottle cap solvent bottles......... . . A0343-010 Solvent bottle cap adaptor .
  • Page 117 Accessories and Replacement Parts Other Parts and Assemblies Tubing, no-ox kit, 31, 22, 18, and 17 cm lengths of low-pressure tubing and fittings (tubing and fittings that connect the degassing unit outlet ports and the proportioning valves) ..00950-01-00021 Tubing, stainless steel, 5 cm length, 0.005 in.

  • Page 119: Appendix A Discontinued Versions Of The Accela Pump

    Discontinued Versions of the Accela Pump There are two versions of the LDA. The differences between the current and previous versions lie in the inlet module, lower displacement chamber, and upper displacement chamber. You can identify the previous version of the LDA by the flow-direction arrow etched onto the front face of the lower displacement chamber.

  • Page 120: Installing The Screw-In Check Valves (Discontinued Version)

    Discontinued Versions of the Accela Pump Installing the Check Valves in the Discontinued LDA To install the check valves and their holders 1. Insert the PEEK film seals into both bores of the lower displacement chamber. 2. Insert the check valves and PEEK seals into their respective holders (see Figure 73).
  • Page 121 Discontinued Versions of the Accela Pump Installing the Check Valves in the Discontinued LDA Figure 74. Installing the PEEK film seals and discontinued check valves Lower displacement chamber Inlet check valve Outlet check PEEK film seals valve 2. Insert the lower displacement chamber into the LDA body just far enough so that the body serves as a vice, and then tighten the check valve fitting with an 8 mm open-ended wrench (see Figure...

  • Page 122: Installing The Components Of The Discontinued Lda Stack

    Discontinued Versions of the Accela Pump Installing the Components of the Discontinued LDA Stack Installing the Components of the Discontinued LDA Stack If they are installed correctly, the inner components of the LDA form a perfect stack that ensures smooth movement of the pistons. To install the inner components of the LDA stack 1.
  • Page 123 Discontinued Versions of the Accela Pump Installing the Components of the Discontinued LDA Stack 5. Using a 4 mm L-hex wrench, tighten the compression lid as shown in Figure Figure 78. Tightening the compression lid Thermo Scientific Accela Pump Hardware Manual...

  • Page 125: Index

    Index description discontinued accessories installing in discontinued LDA kit containing replacing replacement parts checklist stainless steel tubing ChromQuest data system actuating coils, removing configuring an instrument air bubbles configuring the Accela Pump effect on pump pulsation 43, 55, instrument status page for the Accela Pump effect on retention time reproducibility starting and stopping the solvent flow from the pump removing from the piston chambers...
  • Page 126 Index: E delay volume holding hooks determining installing pulse dampener removing specification detector baseline instability inlet blind cartridge, installing performance installation device drivers, ordering checklist discontinued parts 101, connecting the pump to the autosampler dwell volume low-pressure solvent lines See gradient delay volume serial communication cable system interconnect cable unpacking...
  • Page 127 Index: P defined dirty check valves peak area, reproducibility dirty solvent reservoir filters peak shape, effect of plumbing effect of trapped air piston guide bushings monitoring flushing normal installing test failure piston spring clamps, removing pistons cause of scratches quaternary pump, functional description salt buildup volume platform, solvent...
  • Page 128 Index: T steel compression ring step gradient for testing proportioning accuracy Super Flangeless fittings tools required to remove the LDA troubleshooting high backpressure no flow no instrument control no peaks poor peak shape retention time reproducibility tips unstable baseline Troubleshooting Guide tubing connecting pump to autosampler connecting the pump to the autosampler...

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