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Related Manuals for Q Imaging Retiga-SRV
Summary of Contents for Q Imaging Retiga-SRV
- Page 2 4190 Still Creek Drive, Suite 110, Willingdon Business Park Burnaby, BC, Canada V5C 6G9 604.708.5061 QIMAGING QImaging Technical Support Technical support is available to all registered users of QImaging products from 9am to 5pm Pacific Standard Time. SUPPORT QIMAGING 604.708.3068 QIMAGING...
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Page 3: Table Of Contents
Step 3. Connect the Retiga-SRV ................. 5 CAMERA BASICS ......................7 Turning the Camera On and Off ................. 7 Connecting the Retiga-SRV to your Optics ..............7 Understanding the LED Indicators ................8 Capturing Images with the Retiga-SRV ..............8 ADVANCED CAMERA CONTROLS ................ -
Page 4: Introduction
Power Requirements The Retiga-SRV can be powered through a single IEEE 1394 FireWire 6-pin to 9-pin cable when the cooling is off. When the cooling is on, the Retiga-SRV must be connected to the included external power supply. -
Page 5: Ieee 1394 (Firewire) Interface
The Retiga-SRV can be powered and controlled through an IEEE 1394 (FireWire) digital interface. To realise the full bemefits of the Retiga-SRV, a single FireWire connection from the camera to the computer combined with the external power connection will allow full control of the camera and rapid image data transfer from the camera to the computer. - Page 6 QCapture Suite Software The Retiga-SRV operates on both Windows and Mac OS based systems. QCapture Suite Software for both systems is available at . The easy-to-use QCapture software gives you QIMAGING complete control over the camera’s settings and image capture functions. QCapture Suite also includes a TWAIN-compliant interface that allows many Windows image-editing applications to acquire images using QImaging cameras.
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Page 7: Installation
Step 1. Install the 1394 PCI card (Windows Users Only) This step is for Windows PC users only. If you are installing the Retiga-SRV on a Mac OS computer, please skip to Step 2. -
Page 8: Step 2. Install Your Imaging Software
(1) through Windows or Macintosh 6-pin PCI card, (2) through windows or Macintosh laptop computer or (2) through Macintosh 9-pin connection. For the Retiga-SRV, in order to operate the cooling, the camera must have the external power connected. However, should the cooling not be required, a single 9-pin to 6-pin connection will operate the camera sufficiently. - Page 9 Camera Computer Desktop Computer FireWire Connection Figure 3 — • In order to obtain the cooling specification of the camera, the supplied external power adapter needs to be connected to the camera as shown in Figure 4 below. Computer 110/240 VAC Camera power supply FireWire and External Power Supply Connection...
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Page 10: Camera Basics
“Introduction” section. Refer to this step for all system configurations ensuring that the FireWire cable(s) are connected as specified. If power is supplied, the WHITE LED will turn on. To turn the Retiga-SRV off, press the power switch again. This will turn your camera off. The WHITE LED will turn off. -
Page 11: Understanding The Led Indicators
When the temperature is locked and regulated, the blue LED will stay illuminated. It will turn off when cooling is turned off through software. Capturing Images with the Retiga-SRV Consult your imaging application’s user’s manual for more details. -
Page 12: Advanced Camera Controls
The Retiga-SRV offers many advanced features. This section provides information on how to make the most of your QImaging camera. Cooling Capability The Retiga-SRV offers the user the option of selecting the cooling temperature in one (1) degree increments. The maximum cooling of the Retiga-SRV is -30ªC. iGlo (OLED) iGlo is the innovative combination of QImaging’s digital camera technology and an Organic Light... - Page 13 When changing between color states, iGlo will show if the camera is set to Monochrome mode (Figure ?a) or RGB mode (Figure ?b). RGB mode will be displayed on iGlo if Retiga-SRV is a bayer camera or if it is a monochrome camera with an RGB filter attached for color imaging. The icon changes with the change in mode in software.
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Page 14: Triggering The Retiga-Srv Extenally
Bit Depth Icons 7. Sensitivity Mode Retiga-SRV supports two light modes: (1) High Speed mode - for all types of imaging including brightfield, dark-field and fluorescence, offering high frame rates whilst keeping the anti-blooming protection; and (2) High Sensitivity mode - enhances the quantum efficiency above 600nm by reducing the frame rate and turning off anti-blooming protection. - Page 15 There are several ways that you can provide an external pulse to the camera, one of them being via the QImaging External Trigger Board, which is available through QImaging, and another common device is a function generator. To trigger the camera, you need to provide a 5V TTL pulse though the pin stated in Appendix A.
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Page 16: Gain And Offset Controls
Table 1—Trigger Sources and Integration Modes Gain and Offset Controls The Retiga-SRV's electrical gain and offset controls allow the user to map an image's intensities of interest to the camera's digital range. This mapping is performed in the analog domain and thus avoids the quantization errors incurred when the mapping is performed in the digital domain. -
Page 17: Caring For Your Camera
CHAPTER 5 CARING FOR YOUR CAMERA The Retiga-SRV camera requires no regular maintenance except occasional external cleaning of the CCD window (the glass window between the camera sensor and the microscope or lens). To clean the CCD window: CAUTION — The camera’s CCD sensor, and circuits are sensitive to static discharge. -
Page 18: Troubleshooting
• This effect may also be caused by excessive infrared (IR) illumination. Verify that your camera or optical system is blocking the IR. Contact QImaging to order an IR filter. Images That Do Not Appear “Sharp” If the image does not seem “sharp,” check the format of the lens or the coupler being used on the microscope. -
Page 19: Unresolved Problems - Contacting Qimaging Support
Unresolved Problems - Contacting QImaging Support If you are still unable to resolve your problem, contact QImaging Support for assistance in one of four ways: • Visit / for a list of all frequently asked questions. HTTP QIMAGING SUPPORT Your issue may be resolved in one of these faqs. -
Page 20: Glossary
CHAPTER 7 GLOSSARY Binning Binning is a method of increasing camera speed and sensitivity to boost low signals. Binning causes the acquired image to be brighter and smaller, but the resolution will be lower as a result. Because the image is smaller, the image transfer time is reduced significantly. When you select a binning setting in your imaging software, the camera combines data from several pixels in the camera’s CCD into a single super pixel. - Page 21 See IEEE 1394. IEEE 1394 High bandwidth (40 megabytes/second - 1394a; 80 megabytes/second - 1394b) interface for connecting digital imaging, storage, and other devices to host computers. Image Intensifier An electro-optical vacuum tube which intensifies or amplifies on low light level images. Integration The active collection of photons as done by an image sensor.
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Page 22: Appendices
CHAPTER 8 APPENDICES APPENDIX A: External Trigger Port The external trigger port at the rear of the camera is a 6-pin miniature circular DIN receptacle (AMP 749265-1). The mating connector is a Singatron Enterprises part number 62000-6P, available from Digikey (www.digikey.com) External Trigger Port Connector Figure 15 —... - Page 23 Schematic of External Trigger circuit internal to camera. Figure 16 —...
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Page 24: Input Signals
Pin 2 and should be a 5V TTL pulse. Output Signals There are two output signals provided on the Retiga-SRV, SYNC A and SYNC B. SYNC A: This signal indicates the readout time of the camera and this signal is active high. A user could monitor this signal as a means of knowing when to trigger the next frame.