Q Imaging Intensified Retiga Fast 1394 User Manual

Monochrome super high sensivity ieee 1394 firewave digital ccd camera
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  • Page 2 Intensified Retiga Fast 1394 User’s Manual Applicability This document applies to the Intensified Retiga Fast 1394 camera. For the latest updates, please visit QIMAGING Notice of Copyright  Copyright 2003 Quantitative Imaging Corporation. All rights reserved. Unauthorized duplication of this document is prohibited.
  • Page 3: Table Of Contents

    Step 3. Connect the Intensified Retiga Fast 1394 ............5 CAMERA BASICS ......................9 Turning the Camera On and Off ................. 9 Connecting the Intensified Retiga Fast 1394 to your Optics ........9 Understanding the LED Indicators ................10 Capturing Images with the Intensified Retiga Fast 1394 ..........10 ADVANCED CAMERA CONTROLS ................
  • Page 4: Introduction

    A Software Development Kit (SDK) is available upon request for interfacing with custom software. Power Requirements The Intensified Retiga Fast 1394 can be powered through a single IEEE 1394 (FireWire) cable. • Power: 10 watts •...
  • Page 5: Ieee 1394 (Firewire) Interface

    Some computers have 4-pin FireWire ports. These ports may be used if they are OHCI compliant, but you will need to purchase a 4-pin to 6-pin FireWire cable from your local computer store. If you connect the Intensified Retiga Fast 1394 to a 4-pin FireWire port, you likely need to use an external power supply.
  • Page 6 QCapture Suite Software The Intensified Retiga Fast 1394 operates on both Windows and Mac OS based systems. QCapture Suite Software for both systems is available at . The easy-to-use QCapture QIMAGING software gives you complete control over the camera’s settings and image capture functions.
  • Page 7: Installation

    Step 1. Install the 1394 PCI card (Windows Users Only) This step is for Windows PC users only. If you are installing the Intensified Retiga Fast 1394 on a Mac OS computer, please skip to Step 2.
  • Page 8: Step 2. Install Your Imaging Software

    In most cases, the FireWire cable provides the necessary power required by the Intensified Retiga Fast 1394. However, if your camera is cooled you may need to provide additional power to the camera in one of the ways listed in the following figures. Most noteably, you will need to either connect your camera with a 1394l power supply or through an IEEE 1394 HUB.
  • Page 9 Connecting the camera to a PC/MAC desktop or MAC laptop Computer Perform the following for connecting your camera. • Remove the FireWire cable from the camera box, and plug one end of the cable into one of the camera’s FireWire sockets (either socket is fine). FIREWIRE PORTS A U X 1 3 9 4...
  • Page 10 Code 28 or the software not working consistently. In this case, external power must be delivered to the camera which can be accomplished by connecting the camera via one of the following methods. • Connection of an 1394 power supply with your camera is as follows: C om puter C am era 110/240 VA C...
  • Page 11 Computer Port2 Port1 Connected Camera 110/240 VAC Figure 7— FireWire and 1394 Power Supply Connection If you purchased an IEEE 1394 HUB, connect your camera as per the following figure. Camera Computer 1394 HUB 110/240 VAC Figure 8— FireWire and IEEE 1394 HUB Connection 2.
  • Page 12: Camera Basics

    Turning the Camera On and Off To turn the Intensified Retiga Fast 1394 on, all you need to do is to flip the power switch to the “|” position ensuring that you have the appropriate configuration of “Step 3” in the “Introduction”...
  • Page 13: Understanding The Led Indicators

    To attach F-mount optics: If you have a monochrome camera and you have purchased the optional color filter, then carefully thread the color filter to the C-mount ring on the camera. The opposite end of the color filter is a female F-mount connector.
  • Page 14: Advanced Camera Controls

    The Intensified Retiga Fast 1394 has two integration modes: Synchronous and User Controlled. Synchronous mode allows the user to control the Intensified Retiga Fast 1394 in the same way as any other QImaging camera, where all the details of shuttering and gating are handled by the Intensified Retiga Fast 1394’s electronics.
  • Page 15 User Control Gate Mode Timing Diagram Triggering Integration The Intensified Retiga Fast 1394 integration can be triggered either by the camera’s electronics, or externally by the user through the External Trigger Port and Gate Input. When operating in an external...
  • Page 16 Gate Input The Gate Input of the Intensified Retiga Fast 1394 is a BNC connector that accepts fast negative TTL pulses. This port is electrically isolated from the camera's electronics. See Appendix B for further information and schematics of the Gate Input.
  • Page 17: Triggering The Intensified Retiga Fast 1394 Extenally

    The External Trigger Port of the Intensified Retiga Fast 1394 is a 6-pin miniature circular DIN connector. This port accepts digital logic signals that are used to control the camera's integration. The External Trigger Port is optically isolated from the camera's electronics.
  • Page 18: Gain And Offset Controls

    Technical Support. Gain and Offset Controls The Intensified Retiga Fast 1394 has three controls that allow the user to optimize the range of the camera's imaging elements to specific applications. The three controls are Optical Gain, Electrical Gain, and Electrical Offset.
  • Page 19 Diagram of Image Intensifier Tube Assembly Electrical Gain and Offset The Intensified Retiga Fast 1394's electrical gain and offset controls allow the user to map an image's intensities of interest to the camera's digital range. This mapping is performed in the analog domain and thus avoids the quantization errors incurred when the mapping is performed in the digital domain.
  • Page 20: Caring For Your Camera

    CHAPTER 5 CARING FOR YOUR CAMERA The Intensified Retiga Fast 1394 camera requires no regular maintenance except occasional external cleaning of the photomultiplier window (the glass window between the camera sensor and the microscope or lens). To clean the photomultiplier tube window: CAUTION —...
  • Page 21: Troubleshooting

    I am receiving Error Code 10. What does this mean? Ensure you have the correct drivers to operate the camera. The Intensified Retiga Fast 1394 is not supported by driver versions lower than 1.62 for both Macintosh and Windows.
  • Page 22: Unresolved Problems - Contacting Qimaging Support

    When I operate the camera in a Preview mode, my image is black. What is that? The Intensified Retiga Fast 1394 is extremely sensitive to light, however that does not mean that all your settings should be set to the minimum values. If you operate your camera in a Previw Mode, increase the Intensified Retiga Fast 1394 gain and see if this increases the brightness of your image.
  • Page 23: Glossary

    CHAPTER 7 GLOSSARY Binning Binning is a method of increasing camera speed and sensitivity to boost low signals. Binning causes the acquired image to be brighter and smaller, but the resolution will be lower as a result. Because the image is smaller, the image transfer time is reduced significantly. When you select a binning setting in your imaging software, the camera combines data from several pixels in the camera’s CCD into a single super pixel.
  • Page 24 See IEEE 1394. IEEE 1394 High bandwidth (40 megabytes/second - 1394a; 80 megabytes/second - 1394b) interface for connecting digital imaging, storage, and other devices to host computers. Image Intensifier An electro-optical vacuum tube which intensifies or amplifies on low light level images. Integration The active collection of photons as done by an image sensor.
  • Page 25: Appendices

    CHAPTER 8 APPENDICES APPENDIX A: External Trigger Port The external trigger port at the rear of the camera is a 6-pin miniature circular DIN receptacle (AMP 749265-1). The mating connector is a Singatron Enterprises part number 62000-6P, available fromDigikey (www.digikey.com) Figure 14—...
  • Page 26 Figure 15— Schematic of External Trigger circuit internal to camera.
  • Page 27: Appendix B: Gate Input

    Pin 2 and should be a 5V TTL pulse. Output Signals There are two output signals provided on the Intensified Retiga Fast 1394, SYNC A and SYNC B. SYNC A: This signal indicates the readout time of the camera and this signal is active high. A user could monitor this signal as a means of knowing when to trigger the next frame.
  • Page 28 NOTE: You need to provide a ground. Figure 17—Gate Input Connection Gate Input Schematic The following figure illustrates the gate input schematic internal to the Intensified Retiga Fast 1394. Isolated +5V Isolation Buffer 50Ω To Image Intensifier Gate...

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