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Related Manuals for Q Imaging Rolera EM-C2
Summary of Contents for Q Imaging Rolera EM-C2
- Page 2 Rolera EM-C User’s Manual Applicability This document applies to the Rolera EM-C camera. For the latest updates, please visit QIMAGING Notice of Copyright © Copyright 1999-2010 Quantitative Imaging Corporation. All rights reserved. Unauthorized duplication of this document is prohibited. Trademarks and Proprietary Names QImaging, Rolera, Retiga, QICAM, MicroPublisher, Go, LightWire-800, Fast-1394, and QCapture are trademarks of QImaging.
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Page 3: Table Of Contents
CONTENTS Rolera EM-C User’s Manual .................... i INTRODUCTION ......................1 Power Requirements ....................1 Host Requirements ...................... 1 IEEE 1394 (FireWire) Interface ................. 2 Cables..........................2 Imaging Software for Your Camera ................3 INSTALLATION ......................4 Step 1. Install the 1394 PCI-Express card (Windows Users Only) ......4 Step 2. -
Page 4: Introduction
CHAPTER 1 INTRODUCTION The QImaging® Rolera EM-C unites the highest sensitivity of EMCCD sensors with high resolution and high speed. The camera's 1004 x 1002 frametransfer EMCCD provides QE up to 65%, enabling a 1MegaPixel resolution for low-light imaging. With its ability to capture images at an unprecedented 34.2full frames per second in 14-bit mode (and as fast as 200+ frames per second with binning and ROI), the Rolera EM-C sets a new benchmark in high-speed, high-sensitivity performance for live-cell... -
Page 5: Ieee 1394 (Firewire) Interface
For Mac OS Computers: • Intel based Macintosh computers with an OHCI compliant IEEE 1394B port. • Mac OS X 10.5 and above. • 256 MB+ RAM • The included IEEE 1394B card should be installed when a 1394b port is not present. IEEE 1394 (FireWire) Interface The Rolera EM-C can be powered and controlled through an IEEE 1394a or 1394b (FireWire) -
Page 6: Imaging Software For Your Camera
Imaging Software for Your Camera Industry Standard Imaging Applications The Rolera EM-C works with industry-standard Windows and Mac OS imaging software. QCapture Suite Software The Rolera EM-C operates on both Windows and Mac OS based systems. QCapture Suite Software for both systems is available at . -
Page 7: Installation
CHAPTER 2 INSTALLATION IMPORTANT: Follow these steps in order to complete the installation. DO NOT CONNECT the camera until the camera driver is installed. 1. Windows PC users only: Install the IEEE 1394 PCI-Express card - only if necessary. 2. Imaging Software - Install the software and drivers that your new QImaging camera needs to capture images. -
Page 8: Step 2. Install Your Imaging Software
Step 2. Install Your Imaging Software Once the IEEE 1394 card is installed in your computer, you are ready to install the FireWire drivers and imaging software. See your Imaging Software User’s Guide for complete installation instructions. Step 3. Connect the Rolera EM-C Once your imaging software is installed, connect your camera. - Page 9 • Plug the other end of the cable into a FireWire port on your computer Camera Computer Figure 4 — Desktop Computer FireWire Connection • In order to obtain the cooling specification of the camera, the supplied external power adapter needs to be connected to the camera as shown in Figure 5 below. Computer 110/240 VAC Camera...
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Page 10: Camera Basics
CHAPTER 3 CAMERA BASICS The Rolera EM-C ’s image capture capabilities are controlled entirely by your imaging software. This chapter provides basic instructions for working with the camera itself. Turning the Camera On and Off To turn the Rolera EM-C on, press the power switch. -
Page 11: Understanding The Led Indicators
To attach F-mount optics: If you p urchased the optional color filter, then carefully thread the color filter to the C-mount ring on the camera. The opposite end of the color filter is a female F-mount connector. Carefully connect your F-mount optics to the open connector of the color filter until it is mounted securely. Use your optics controls to adjust focus. -
Page 12: Advanced Camera Controls
CHAPTER 4 ADVANCED CAMERA CONTROLS The Rolera EM-C offers many advanced features. This section provides information on how to make the most of your QImaging camera. Cooling Capability The Rolera EM-C offers the user the option of selecting the cooling temperature in one (1) degree increments. - Page 13 Connecting External Sources to the Camera Before you can begin to externally trigger your camera, you need to connect the necessary trigger signal source to the camera. To do this you need to ensure that you have a 6-pin miniature circular din cable and ensure that the pinouts are set correctly from your trigger source.
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Page 14: Gain And Offset Controls
Gain and Offset Controls The Rolera EM-C has two gain modes: (1) Multiplication Gain or EM Gain and (2) Easy-EM mode . The Multiplication gain controls the EM portion of the camera and allows you to bring the signal of the sample above the read-noise. -
Page 15: Caring For Your Camera
CHAPTER 5 CARING FOR YOUR CAMERA The Rolera EM-C camera requires no regular maintenance except occasional external cleaning of the cooling window (the glass window that forms part of the cooling chamber and sits between the camera sensor and the microscope or lens) . To clean the Cooling window: CAUTION —... -
Page 16: Troubleshooting
CHAPTER 6 TROUBLESHOOTING Resolving Problems with the camera The White LED is not lit. • Check all the cable connections. • Press the switch at the back of the camera. • I f your white LED is still not lit, then your camera may not be receiving the required power. If you have a 1394 Power Supply or the included external power supply, connect the camera as listed in Step 3 of the “Intr oduction ”... -
Page 17: Unresolved Problems - Contacting Qimaging Support
Unresolved Problems - Contacting QImaging Support If you are still unable to resolve your problem, contact QImaging Support for assistance in one of four ways: • Visit http://www.qimaging.com/support/faq/index.php for a list of all frequently asked questions. Your issue may be resolved in one of these faqs . •... -
Page 18: Glossary
GLOSSARY Binning Binning is a method of increasing camera speed and sensitivity to boost low signals. Binning causes the acquired image to be brighter and smaller, but the resolution will be lower as a result. Because the image is smaller, the image transfer time is reduced significantly. When you select a binning setting in your imaging software, the camera combines data from several pixels in the camera ’... - Page 19 IEEE 1394 High bandwidth (40 megabytes/second - 1394a; 80 megabytes/second - 1394b) interface for connecting digital imaging, storage, and other devices to host computers. Image Intensifier An electro-optical vacuum tube which intensifies or amplifies on low light level images. Integration The active collection of photons as done by an image sensor.
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Page 20: Appendices
APPENDICES APPENDIX A: External Trigger Port The external trigger port at the rear of the camera is a 6-pin miniature circular DIN receptacle (AMP 749265-1). The mating connector is a Singatron Enterprises part number 62000-6P, available fr om Digikey (www.digikey.com) Figure 15 —... - Page 21 CN8 PIN REFERENCE 1 : USER VCC 2 : TRIGGER (input) 3 : SYNCA (output) 4 : GROUND 357R R32 357R 5 : SYNCB (output) 6 0.1uF TRIGGER 6 : GROUND R33 357R HCPL-061A MINI-DIN 357R R35 237R 0.1uF SYNCA R36 237R HCPL-061A 357R...
- Page 22 Input Signals In order to use the External Trigger Port correctly and ensure external triggering operation for your camera, you need to supply at least 4 input signals through the mini din receptacle. Pin 1 always needs to be powered at a 5V potential, with Pins 4 and 6 always connected to ground. The input signal to begin triggering the camera is through Pin 2 and should be a 5V TTL pulse.