Q Imaging Retiga EXi User Manual

Very high sensitivr ieee 1394 firewire digital ccd camera - monochrome or color

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Summary of Contents for Q Imaging Retiga EXi

  • Page 2 Retiga EXi Fast 1394 User’s Manual Applicability This document applies to the Retiga EXi Fast 1394 camera. For the latest updates, please visit QIMAGING Notice of Copyright  Copyright 2003 Quantitative Imaging Corporation. All rights reserved. Unauthorized duplication of this document is prohibited.
  • Page 3: Table Of Contents

    Step 3. Connect the Retiga EXi Fast 1394 ..............5 CAMERA BASICS ......................9 Turning the Camera On and Off ................. 9 Connecting the Retiga EXi Fast 1394 to your Optics ..........9 Understanding the LED Indicators ................10 Capturing Images with the Retiga EXi Fast 1394 ............10 ADVANCED CAMERA CONTROLS ................
  • Page 4: Introduction

    CHAPTER 1 INTRODUCTION The QImaging Retiga EXi CCD digital camera features enhanced visible and IR quantum efficiency for super high sensitivity for demanding low light and fluorescence imaging applications. A progressive scan interline CCD sensor gives a resolution of 1.4 million pixels in a 12-bit digital output. High-speed low-noise electronics provide FireWire linear digital data for rapid image capture.
  • Page 5: Ieee 1394 (Firewire) Interface

    Some laptop computers have 6-pin FireWire jacks that do not provide power. If your computer has such a port, you will have to power your Retiga EXi Fast 1394 by attaching an external power supply to the system.
  • Page 6 QCapture Suite Software The Retiga EXi Fast 1394 operates on both Windows and Mac OS based systems. QCapture Suite Software for both systems is available at . The easy-to-use QCapture software QIMAGING gives you complete control over the camera’s settings and image capture functions. QCapture Suite also includes a TWAIN-compliant interface that allows many Windows image-editing applications to acquire images using QImaging cameras.
  • Page 7: Installation

    Step 1. Install the 1394 PCI card (Windows Users Only) This step is for Windows PC users only. If you are installing the Retiga EXi Fast 1394 on a Mac OS computer, please skip to Step 2.
  • Page 8: Step 2. Install Your Imaging Software

    Once the IEEE 1394 card is installed in your computer, you are ready to install the FireWire drivers and imaging software. See your Imaging Software User’s Guide for complete installation instructions. Step 3. Connect the Retiga EXi Fast 1394 Once your imaging software is installed, connect your camera. There are two different types of connections.
  • Page 9 Connecting the camera to a PC/MAC desktop or MAC laptop Computer Perform the following for connecting your camera. • Remove the FireWire cable from the camera box, and plug one end of the cable into one of the camera’s FireWire sockets (either socket is fine). FIREWIRE PORTS Figure 3—...
  • Page 10 Code 28 or the software not working consistently. In this case, external power must be delivered to the camera which can be accomplished by connecting the camera via one of the following methods. • Connection of an 1394 power supply with your camera is as follows: C om puter C am era 110/240 VA C...
  • Page 11 Computer Port2 Port1 Connected Camera 110/240 VAC Figure 7— FireWire and 1394 Power Supply Connection If you purchased an IEEE 1394 HUB, connect your camera as per the following figure. Camera Computer 1394 HUB 110/240 VAC Figure 8— FireWire and IEEE 1394 HUB Connection 2.
  • Page 12: Camera Basics

    Turning the Camera On and Off To turn the Retiga EXi Fast 1394 on, all you need to do is to flip the power switch to the “|” position ensuring that you have the appropriate configuration of “Step 3” in the “Introduction” section. Refer to this step for all system configurations ensuring that the FireWire cable(s) are connected as specified.
  • Page 13: Understanding The Led Indicators

    If you turn off cooling (a setting in your imaging software), the amber LED will turn off too. Capturing Images with the Retiga EXi Fast 1394 Consult your imaging application’s user’s manual for more details.
  • Page 14: Advanced Camera Controls

    The External Trigger Port of the Retiga EXi Fast 1394 is a 6-pin miniature circular DIN connector. This port accepts digital logic signals that are used to control the camera's integration. The External Trigger Port is optically isolated from the camera's electronics.
  • Page 15: Gain And Offset Controls

    Table 1—Trigger Sources and Integration Modes Gain and Offset Controls The Retiga EXi Fast 1394's electrical gain and offset controls allow the user to map an image's intensities of interest to the camera's digital range. This mapping is performed in the analog domain and thus avoids the quantization errors incurred when the mapping is performed in the digital domain.
  • Page 16: Caring For Your Camera

    CHAPTER 5 CARING FOR YOUR CAMERA The Retiga EXi Fast 1394 camera requires no regular maintenance except occasional external cleaning of the CCD window (the glass window between the camera sensor and the microscope or lens). To clean the CCD window: CAUTION —...
  • Page 17: Troubleshooting

    If the image does not seem “sharp,” check the format of the lens or the coupler being used on the microscope. Retiga EXi Fast 1394 is equivalent to the CCD sensor and requires the appropriate lens or coupler. An incorrect coupler on the microscope will not provide the correct field of view, and will...
  • Page 18: Unresolved Problems - Contacting Qimaging Support

    Unresolved Problems - Contacting QImaging Support If you are still unable to resolve your problem, contact QImaging Support for assistance in one of four ways: • Visit / for a list of all frequently asked questions. HTTP QIMAGING SUPPORT Your issue may be resolved in one of these faqs. •...
  • Page 19: Glossary

    CHAPTER 7 GLOSSARY Binning Binning is a method of increasing camera speed and sensitivity to boost low signals. Binning causes the acquired image to be brighter and smaller, but the resolution will be lower as a result. Because the image is smaller, the image transfer time is reduced significantly. When you select a binning setting in your imaging software, the camera combines data from several pixels in the camera’s CCD into a single super pixel.
  • Page 20 See IEEE 1394. IEEE 1394 High bandwidth (40 megabytes/second - 1394a; 80 megabytes/second - 1394b) interface for connecting digital imaging, storage, and other devices to host computers. Image Intensifier An electro-optical vacuum tube which intensifies or amplifies on low light level images. Integration The active collection of photons as done by an image sensor.
  • Page 21: Appendices

    CHAPTER 8 APPENDICES APPENDIX A: External Trigger Port The external trigger port at the rear of the camera is a 6-pin miniature circular DIN receptacle (AMP 749265-1). The mating connector is a Singatron Enterprises part number 62000-6P, available fromDigikey (www.digikey.com) Figure 11—...
  • Page 22 Figure 12— Schematic of External Trigger circuit internal to camera.
  • Page 23: Input Signals

    Pin 2 and should be a 5V TTL pulse. Output Signals There are two output signals provided on the Retiga EXi Fast 1394, SYNC A and SYNC B. SYNC A: This signal indicates the readout time of the camera and this signal is active high. A user could monitor this signal as a means of knowing when to trigger the next frame.

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