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10. Using the focusing control of the Centering Telescope only, focus the rings to
maximum clearness.
11. To achieve optimum performance, the bright ring must be positioned concen-
tric with the darker ring at the center of view. This is done by using the knurled
ring holding the illumination annulus located underneath the turret housing.
Move the knurled ring until the bright ring is concentric with the dark ring.
(See the right chart)
12. Put the green filter onto the top of the illuminator condenser frame.
13. Replace Centering Telescope with normal eyepiece. You can observe an excel-
lent phase contrast view of your specimen.
14. STEP 9-12 should be performed for the other objective lenses.
Note)
1:
The phase turret dial number must match the magnification power of each
objective. 10=10X, 20=20X, 40=40X, 100=100X
2:
Use slides which are free of dust and 1mm thick with cover slips 0.17mm
thick.
3:
Care should be taken not to disturb the illumination annulus under the tur-
ret of the phase condenser after it has been adjusted (STEP 11), otherwise
misalignment may occur.
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