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PHASE CONTRAST SYSTEM
Phase contrast is a form of illumination which alters the normal path of light waves
from an object to a wavelength shifted diffraction pattern thus allowing visualization
of transparent or low contrast specimens. This allows living cells and suspensions to
be viewed without the need for staining procedures.
Phase Set Components
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Phase Turret Condenser, N.A 1.25
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Achromat or Plan Phase Objectives (DM)
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10X NA=0.25
20X NA=0.40
40X NA=0.65
100X (oil) NA=1.25
Centering Telescope
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Green Filter
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Installation Key (Screwdriver)
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Storage Case
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INSTALLATION AND ALIGNMENT
1.
Replace the normal brightfield objectives of the microscope with the phase ob-
jectives (Dark & Medium type) supplied in the storage case.
2.
Remove the brightfield substage condenser by loosening the condenser locking
thumb screw and gently lowering the condenser down and out of the mounting
ring. This thumb screw is located on the left side of the condenser mount as
you face the front of the microscope.
3.
Install the Phase Turret Condenser by sliding it up into the condenser mount
and tightening the condenser locking thumb screw using the Installation Key
provided. The index mark (white dot) on the turret condenser should be situ-
ated at the front of the microscope.
4.
Rotate the turret dial of the condenser to align the zero (0) mark to the index
mark on the turret housing. This is the setting to use for any brightfield work.
5.
Raise the condenser to its maximum height and place a specimen slide on the
stage.
6.
Swing the 10X objective into the optical path and focus.
7.
Move the slide, using the mechanical stage, to a location with little or nothing
in the field of view.
8.
Rotate the turret dial of the condenser to align the 10 mark to the index mark
(white dot) on the turret housing.
9.
Replace either eyepiece with the Centering Telescope. Looking through the
telescope, you can see two rings of light, one at the center of view, the other
slightly off center.
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