Chapter 1 Qx200 ™ Droplet Generator; Introduction - BIO RAD QX200 Instruction Manual

1
QX200
Generator

1.1 Introduction

The QX200 ™ Droplet Digital
precise digital PCR. The system consists of two instruments, the QX200 droplet
generator and the QX200 droplet reader, and their associated consumables.
The QX200 droplet generator partitions samples into 20,000 nanoliter-sized
droplets and, after PCR on a thermal cycler, droplets from each sample are
analyzed individually on the QX200 droplet reader. PCR-positive and PCR-
negative droplets are counted to provide absolute quantification of target DNA
in digital form. Alternatively, amplified products can be extracted from droplets
following PCR for downstream applications, such as sequencing or cloning.
The ddPCR system lets you:
Detect rare DNA target copies with unmatched sensitivity
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Determine copy number variation with unrivaled accuracy
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Measure gene expression levels with precision
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Applications and uses include:
Copy number variation
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Rare sequence detection
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Gene expression analysis
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Next-generation sequencing (NGS)
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library quantification
Viral load determination
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This manual covers use of the QX200 droplet generator and preparation for PCR.
For information on the QX200 droplet reader, please refer to bulletin 10031906.
Droplet
™
™ PCR (ddPCR ™
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QX200 Droplet Generator Instruction Manual
) system performs accurate and
Single cell gene expression analysis
Absolute quantification
Rare mutant detection
miRNA analysis
NGS sample preparation
GMO detection
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