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Q Imaging Retiga 3000 User Manual

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  • Page 1 www.QIMAGING.com...
  • Page 2 ® are registered trademarks of Roper Scientific, Inc. Product names mentioned in this document may be trademarks or registered trademarks of QImaging or other hardware, software or service providers, and are used herein for identification purposes only. Microsoft and Windows are registered trademarks in the U.S.
  • Page 3 Product Warranty Plan is provided on all new and used equipment, including retired demonstration cameras. Extended Product Warranty Plan Extended Product Warranty Plans are conveniently priced and very easy to purchase. Available for all QImaging cameras, the Extended Product Warranty Plan includes parts and labor, and is available in one-year increments up to five years.
  • Page 4 Retiga 3000 USER MANUAL RETIGA 3000 CERTIFICATE OF CONFORMITY www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

  • Page 5: Table Of Contents

    Installation Step 1: Install the USB 3.0 PCIe Card ..............5 Installation Step 2: Install the Camera Driver ............... 5 Installation Step 3: Connect the Camera ................6 USING YOUR RETIGA 3000 CAMERA ................7 Imaging Software ....................... 7 Basic Camera Parameters ....................7 Exposure Time ........................

  • Page 6: Introduction

    75%, this camera provides higher resolution and sensitivity than most standard fluorescence microscopy cameras. Available in monochrome or color, the Retiga 3000 uses a USB 2.0 data connection for a fast and easy installation. Additionally, low-noise electronics, cooled sensor technology, and excellent quantum efficiency (QE) yield superb sensitivity for low-light fluorescence applications.

  • Page 7: Precautions

    0°C to 30°C with 80% relative humidity, noncondensing. Store the Retiga 3000 camera system in its original containers. To protect the system from excessive heat, cold, and moisture, store at an ambient temperature between -20°C and 60°C with a relative humidity of 0% to 90%, noncondensing.

  • Page 8: Cleaning

    Retiga 3000 USER MANUAL Cleaning Clean the exterior surfaces of the camera with a dry, lint-free cloth. To remove stains, contact QImaging Support. To clean the camera’s imaging window, use only a filtered compressed-air source. Handheld cans are not recommended, as they may spray propellant onto the window. Do not touch the window.

  • Page 9: Installation

    Retiga 3000 USER MANUAL CHAPTER 2 INSTALLATION This chapter will detail the proper installation of your Retiga 3000 camera. In order to install and use the camera, the following items are required: Retiga 3000 camera (supplied) ■ USB 2.0 data cable (supplied) ■...

  • Page 10: Camera Power Requirements

    Installation Step 2: Install the Camera Driver In order for the Retiga 3000 to communicate with the host PC, the camera’s device driver (PVCAM) must first be installed. Before connecting the camera to the computer, use the supplied USB flash drive to run the PVCAM installer executable.

  • Page 11: Installation Step 3: Connect The Camera

    Installation Step 3: Connect the Camera In addition to a power switch, there are three connectors on the back of the Retiga 3000 camera: a USB 2.0 connector, a power supply connector, and an I/O connector. Connect the camera following these steps: IMPORTANT: Ensure the power switch is set to the OFF position and before connecting the camera 1.

  • Page 12: Using Your Retiga 3000 Camera

    Basic Camera Parameters Exposure Time The Retiga 3000 camera’s exposure controls allow you to adjust the integration time for each acquisition (0 ms – 30 min). By increasing the exposure time, more light is captured by the sensor and a better signal-to-noise ratio (SNR) is achieved.

  • Page 13: Sensor Cooling

    +5°C. Clocking Mode The Retiga 3000 camera has two CCD clocking modes, one of which allows removal of the glow that can appear in an image’s corners at longer exposures (arising from preamplifiers). In the PVCAM implementation, the clocking modes are referred to as “Normal”...

  • Page 14: Clearing Mode

    However, since exposure and readout occur simultaneously, the minimum exposure time is dependent on the time taken to complete readout. As such, excluding the first frame, any exposure time smaller than the readout time defaults to an exposure duration equal to the readout time. www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

  • Page 15: Roi / Binning

    A special cable is available to access primary signals such as “Trigger-in,” “Expose out,” and “Frame Readout”. In the default mode, the camera triggers on the rising edge of a TTL signal. Retiga 3000 cameras support three trigger modes: Trigger-first mode In this mode, the camera requires one trigger to begin the acquisition of a stream of images.
  • Page 16 In this mode, each frame in the sequence requires a trigger. The camera exposes for the duration the trigger signal is high. Exposure time entered into the software is not used in this mode. Triggers received when Trigger Ready is low are ignored. Non-Overlap mode is shown below. www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...
  • Page 17 ■ Clear Pre-Exposure ■ Clear Pre-Exposure Gamma = 1 ■ ■ Gamma = 1 10ms exposure ■ ■ 30ms exposure RGB values ■ ■ R = 1.6, G = 1, B = 1.5 www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

  • Page 18: Troubleshooting

    Confirm that the camera is operational by taking an image with a standard C-mount lens attached ■ to your Retiga 3000. Using normal room lighting, place the camera on a table about 3 meters away from an object and acquire an image.

  • Page 19: Unresolved Problems - Contacting Qimaging Support

    (and at least one inch of space has been left around the camera housing), switch off the camera immediately and contact QImaging Support. Unresolved Problems - Contacting QImaging Support If you are still unable to resolve your problem, contact QImaging Support for assistance in one of four ways: http://www.qimaging.com/support/faq/ Visit for a list of all frequently asked questions.

  • Page 20: Specifications

    2.465 [62.61] 5.756 [146.2] Additional Camera Measurements Dimensions: 97.79 mm x 125.22 mm x 146.2 mm Camera weight: 3.10 lbs (1.406 kg) Optical interface: 1”, C-mount optical format Mounting hole thread size: 1/4”-20 thread www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

  • Page 21: Connectors On Back Of Camera

    Hirose Electrical Co. Connector Part # HR212-10P-10PC Power Supply Specifications IMPORTANT: Only operate the Retiga 3000 camera with the power supply provided by QImaging. Voltage output: +5 V DC, 4 A Voltage input: 100 – 240 V @ 50 – 60 Hz IMPORTANT: Must not exceed 5V DC, 4A.

  • Page 22: Focal Plane Measurement

    1940 x 1460 (2.8 MP) Pixel size: 4.54 µm x 4.54 µm Single-pixel full well: 16,000 e- (24,000e- binned 2x2) Peak QE: 75% @ 600 nm (measured using monochrome model) Readout frequency: 20 MHz Cooling: 0°C (stabilized) www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

  • Page 23: Sensor Orientation

    USED IN THE 6000 When the Retiga 3000 is coupled to the side port of an inverted microscope with the flat surface of the camera parallel to the table, the CCD orientation will match the view seen through the eyepiece. This allows for easy access to the mounting holes to stabilize the camera while maintaining a consistent perspective between the captured images and what’s seen down the eyepiece of the microscope.
  • Page 24 Power Ready – reflects power status for the camera (+5 V = on, 0 V = off) ■ Ground – system digital ground ■ Types of triggering supported by the Retiga 3000: Trigger-first mode (Overlap/Non-Overlap) ■ Strobe mode (Overlap/Non-Overlap) ■...

  • Page 25: Appendix A: Usb Information

    USB 2.0 Chipset Incompatibility Notice The USB 2.0 hardware used in the Retiga 3000 cameras is incompatible with new computers using native Intel USB 3.0 host controllers. A third party PCIe USB 3.0 card is required to resolve the problem.

  • Page 26: Determining If A Usb Port Is On A Root Hub

    Each port can have a device plugged in or another hub to allow connection of more devices. However, the Retiga 3000 camera should be the only device connected to a single USB Root Hub. If other devices are connected to the same Root Hub, then camera communication may fail, which can result in camera hanging or image tearing.
  • Page 27 If more devices are connected to the same USB Root Hub, please reconnect them to a different port for optimal camera performance or use the supplied USB 3.0 card. For any further questions and assistance, please contact QImaging technical support. www.qimaging.com ©2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...
  • Page 28 +1 800.874.9789 support@qimaging.com www.qimaging.com © 2014 QImaging. All rights reserved. QI_Retiga3000_UM_Rev_A1...

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