RULE 3: IF IN DOUBT; ASK FOR HELP A. START-UP 1. Turn on the visual display by turning toggle switch DISPLAY POWER to 1. (These toggle switches must be pulled out slightly before switching - DO NOT FORCE THEM) 2. Turn the toggle switch SC AIRLOCK VALVE from CLOSED to AUTO (OPEN)- a few seconds later the green light on the HV off/ready display will come on.
7. Set magnification to IQOOX. You can do this by manually turning the MAGNIFICATION knob or pressing the PRESET switch on the magnification display. 8. Locate area of interest using X and V stage shifts (you may need to go to low magnification see section I).
4. Wait for the line scan to perform on cycle and expose the film. Exposure is complete when light on the DIRECT switch goes off. 5. Withdraw the film from the cassette. WHAT TO DO WHEN IT ALL GOES DARK - DON'T PANIC: DON'T FIDDLE WITH ANYTHING 1.
Hit ENTER to move $ sign against STIG ALIGN Y Press ENTER again 11. The image will oscillate on the screen, minimise oscillation with ALIGNMENT X and Y knobs. 12. Press PF16 EXIT to finish. CHANGING THE DETECTOR The 4500 has two secondary electron detectors, an upper detector which sits above the specimen and a lower detector which sits to the side of the specimen.
Often it can be difficult to locate the area you want on a specimen. In this case use Low Magnification Imaging, here the lowest magnification is x20. 1. Press LOW MAG switch on display panel (the green light in the switch will glow). This will automatically set the magnification to x500.
FINISHING A SESSION 1. Turn the SC AIRLOCK VALVE from AUTO (OPEN) to CLOSED - the green light on the HV off/ready display will turn off.. 2. Turn off the visual display by turning DISPLAY POWER to O. 3. Fill in log book.