2. Operating the equipment Installation ........................20 2.1.1 Installing the liquid nitrogen supply ..............20 Loading printer paper (MRV only) ................21 Using a Planer LNP4 pump and Dewar ............... 22 2.3.1 Filling the Dewar ....................22 2.3.2 Fitting the pump ....................22 2.3.3...
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Table of Contents 2.6.5 Removing the samples ..................29 Reports .......................... 30 Switching off the system (MRV only) ................30 Switching off the system (MR7 only) ................30 3. Routine maintenance and troubleshooting Routine maintenance ....................34 Calibrating the system ....................35 Troubleshooting ......................
TW16 7HD, UK. Tel: +44 (0)1932 755000; email: sales@planer.com Any serious incidents that may occur with this device should be reported to Planer Ltd and the competent authority of the Member State in which the user and/or patient is established. If the incident occurs within the EU, the EC REP identified on the device should also be informed.
Introduction Symbols 1.2.1 Symbols used in this manual Warning. Information or instructions that are related to safety. Failure to follow these instructions may result in personal or third-party injury. Caution. Important information or instructions related to the safe use of the equipment.
Introduction 1.2.3 Symbols used on the equipment (MRV only) Caution: consult instructions for use. Failure to follow these instructions may result in personal or third-party injury. Caution: low temperature or freezing conditions. Caution: hot surface. Catalogue number. Manufacturer. Outlet duct. Mains ON Mains OFF Alternating current...
Introduction Safety precautions Warning Ensure that you have read and fully understand these instructions before attempting to use this equipment. 1.3.1 First aid IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any quantity of liquid nitrogen comes into contact with the skin or eyes, immediately flush that area of skin with large quantities of tepid water.
Introduction The Series 500 chamber weighs 23 kg and requires at least two people for safe handling. o Support the weight using the bottom plate. o Do not attempt to lift using the edges of the case. Cautions The connectors on the equipment must only be used for connection to other equipment described in this document.
Introduction o Liquid nitrogen boils at -196 ºC and both liquid and gas can cause rapid and severe frostbite. o Delicate tissue such as the eyes can be damaged by exposure to cold gas, which may be too brief to affect the skin of the hands or face. Stand clear of boiling and splashing liquid nitrogen and its gas when filling a Dewar or when inserting objects, such as a pump, into the liquid.
Introduction Cryopreservation process to enable the samples to be re-introduced into the body at a later date. Theory of operation The equipment is designed to cool samples by following a predefined time-temperature profile. The temperature-time relationship is referred to as the profile. The equipment comprises two main elements: 1.
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Introduction Fig.1 Log in screen Fig.2 Menu screen Series 300-M & 500-M: Instructions for use MA102747-M-EN 10.0.2697...
Introduction 1.6.1.1.1 Connection overview 1.6.1.1.1.1 MR7 controller All connectors are at the bottom of the MR7 system control box. Fig.3 Connections on the MR7 control box DC power input Controller rate freezer connector. Primary temperature probe input Secondary temperature probe input Serial port.
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Introduction Fig.4 Connections for the 300 series freezers Fig.5 Connections for the 500 series freezers Series 300-M & 500-M: Instructions for use MA102747-M-EN 10.0.2697...
Introduction 1.6.1.1.1.2 Chamber All connectors are located at the back of the freezer. Fig.6 Connections on the back of the 300 series freezer Liquid nitrogen inlet INPUT SAMPLE Sample temperature probe input LNP4 Power outlet for LNP4 pump only ONLY MAINS Mains power inlet INLET...
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Introduction Fig.7 Connections on the back of the 500 series freezer Liquid nitrogen inlet INPUT SAMPLE Sample temperature probe input Power outlet for MRV and LNP4 pump only POWER MAINS Mains power inlet INLET Series 300-M & 500-M: Instructions for use MA102747-M-EN 10.0.2697...
Introduction 1.6.2 Guide to the MRV controller Key: 1. Contrast Manual override: heat Manual override: cool 1.6.2.1 Screen layout To select a menu item, press and then 1.6.3 Connections Warning Installation must only be undertaken by suitably trained personnel. Typical connections to a Series 300 system are shown below. Key: 1.
Operating the equipment Operating the equipment Installation Warning Installation must only be undertaken by suitably trained personnel. Ensure that you have all the latest updates to the IFU, Installation Qualification and MR7 software version before installation.. Any electrical devices connected to the equipment must comply with either IEC 950, IEC 61010 or their equivalent.
Operating the equipment Your service provider will connect the chamber to a liquid nitrogen cylinder or a Planer LNP4 pump and Dewar. Loading printer paper (MRV only) Note Only use thermal paper recommended by Planer Limited or an authorised distributor.
Operating the equipment Using a Planer LNP4 pump and Dewar This section describes how to use the Planer LNP4 pump and dewar. 2.3.1 Filling the Dewar Warning Wear protective clothing. See Liquid nitrogen and vessels. Never remove a pump from a Dewar until the pressure gauge reading has fallen to zero.
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Operating the equipment 3. Press firmly on the top of the pump to hold it level and clip all the springs onto the Dewar. Alternate between handles. 4. Close the pressure relief valve. 5. Connect the mains inlet of the pump power supply unit to the auxiliary power output on the rear of the chamber.
Operating the equipment Caution Always test the Dewar after fitting the pump. 2.3.3 Testing and pressuring the Dewar Warning Wear protective clothing. See Liquid nitrogen and vessels. 1. Connect the mains lead from the chamber to an appropriate wall socket. 2.
Operating the equipment 2.3.4 Removing the pump You will need to remove the pump whenever the Dewar needs to be refilled. Warning Never remove a pump from a Dewar until the pressure gauge reading has fallen to zero. Wear protective clothing. See Liquid nitrogen and vessels.
Operating the equipment Configuring the controller (MRV only) 2.4.1 Configuring the system Note Default passwords are 1111. 1. Select Configure from the main menu. 2. Select Set Time Date and enter the correct time and day. Use a 24 hour clock and enter the date in dd/mm/yyyy format.
Preparing the nitrogen supply Caution Ensure that you have enough liquid nitrogen to complete your freezing run. If using a Planer pump and dewar, follow the instructions in Using a Planer LNP4 pump and dewar If using a cylinder, refer to the manufacturer's instructions on how to switch on the supply.
Operating the equipment 3. Select how you want to run the profile: a. If you intend to run the profile using DeltaT, select Control from PC and then refer to the DeltaT documentation. b. If running directly from the controller, select profile.
Operating the equipment 2.6.4 Loading the samples using an MRV Warning Use tongs or wear suitable protective gloves when handling cold or hot objects. 1. Load the samples. To silence the beeping, press any key other than the key. 2. After loading the samples, wait five minutes to allow the chamber to re-stabilise. 3.
Operating the equipment Caution Switching off the chamber at sub-zero temperatures may cause serious damage to the equipment. 4. When prompted to return the chamber to room temperature, select to rewarm the chamber. Only select if you intend to run another profile starting at a very low temperature.
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Operating the equipment 2. Click Shut down 3. Wait (approximately 20 seconds) for the green LED to go off on the controller before removing power. 4. Switch off the system using the mains switch on the chamber’s control panel 5. If resuming power to the unit please wait 10 seconds after the previous shut down.
Routine maintenance and troubleshooting Routine maintenance and troubleshooting Routine maintenance Warning Before cleaning: o Switch off the equipment. o Disconnect the mains supply at the wall socket. o Turn off any connected liquid nitrogen cylinders. o Open the red pressure relief valve on the pump. Wait until the pressure on the gauge has fallen to zero and the flow of gas has stopped.
Routine maintenance and troubleshooting To ensure adequate earth bonding, the equipment and mains cables must be regularly checked by suitably trained personnel using a Portable Appliance Tester or similar equipment. The earth continuity of the mains installation must be regularly inspected by the person responsible for the safety of the installation.
Check the nitrogen supply. Check the fuses. If using a Planer LNP4cpump, check that the pump filter is not blocked by ice. 3.3.4 The chamber will not heat Warning Refer to the safety instructions at the front of this manual.
3.3.7 Returning for service Should the equipment or any part of the equipment need to be sent back to Planer Limited for repair, or if the equipment is to be inspected, maintained or repaired on-site by Planer Limited personnel, a Decontamination Certificate must be completed. This can be downloaded from http://www.planer.com/support/service/decontamination-...
Additional information Additional information Specifications 4.1.1 System specifications Accuracy ±(0.3 + 0.005 x TM) °C (where TM is the magnitude of the temperature). Storage temperature -10 °C to +50 °C Storage humidity 5% to 95% relative humidity non-condensing Operating environment For indoor use only Operating temperature +5 °C to +40 °C...
Additional information 4.1.2 MRV Controller specifications Feature Heating rates 0.01 to 10 °C/min Cooling rates -0.01 to -50 °C/min * Temperature range +40 to -180 °C/min Printer 320/640 dot thermal printer STP411 832 dot thermal printer LTPV445 PC interface to DeltaT RS232 Dimensions 80 mm high x 220 mm wide x 350 mm deep...
Additional information 4.1.3 MR7 Controller specifications Feature Value Heating rates 0.01 to 10 °C/min Cooling rates -0.01 to -50 °C/min * Temperature range +40 to -180 °C/min Dimensions 242mm wide x 205mm deep x 228mm high Weight 1.8 kg approx. Display 7.1"...
Additional information 4.1.4 Chamber specifications Kryo chamber 360-1.7 360-3.3 560-16 Weight kg 14.4 14.7 Capacity litres Dimensions mm 450 x 300 x 420 450 x 300 x 420 470 x 650 x 420 (External H x W x D) Dimensions mm 200 x Ø150 400 x Ø150 310 x 226 x 230...
Additional information Kryo 360-1.7 and 360-3.3 F1 and F2 115V ~ model T 10A H 250V T 5A L 250V (Planer # FL013299) (Planer #FL013801) 230 V~ model T 5A L 250V T 2.5A L 250V (Planer #FL013801) (Planer #FL012026)
INDEX dewar - A - first aid fitting pump alarm fuses external - I - - C - indications for use calibrating installation chamber liquid nitrogen connectors specification - K - configuring system connections keys connectors - L - chamber controller controller liquid nitrogen...
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INDEX dewar - R - theory of operation troubleshooting display blank removing no response pump run stopped samples will not cool reports will not heat reset returning - U - for service routine maintenance unexpected reset starting - S - safety equipment liquid nitrogen...
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Planer Limited, 110 Windmill Rd., Sunbury-on-Thames, Middlesex, TW16 7HD, UK. www.planer.com Tel: +44 (0)1932 755000 Series 300-M & 500-M: Instructions for use MA102747-M-EN 10.0.2697...
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