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Table of Contents
Troubleshooting
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Summary of Contents for Planer Kryo550-16
- Page 1 Kryo550-16 Operator’s Manual...
- Page 2 Hard copies and backup copies should be routinely kept. All equipment supplied by Planer plc. is subject to a Warranty Agreement and Limited Liability. In addition, software products are not sold but are licensed for use and are subject to a Licence Agreement.
- Page 3 Safety i Symbols Used in this Manual Failure to comply with these instructions could result in personal injury. Failure to follow these instructions could result in damage to your equipment, system or samples. This indicates first aid advice.
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Page 4: Symbols Used On The Chamber
ii Safety Symbols Used on the Chamber Run key Stop key Sample display key Heat manual override Cool manual override Chamber PRT Sample PRT Reset Alarm output Ethernet 10 Base T RS232 serial port Mains power off Mains power on Caution - Read this manual. -
Page 5: Safety Precautions
(such as a pump) into the liquid. Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available as an accessory from Planer Biomed. When running a program, the chamber and contents may get cold enough to cause frostbite. -
Page 6: Iv Safety
The 9-way D Type plug on the Kryo550-16 interface panel is only for connecting to the RS232 serial port of a computer which complies with IEC950. - Page 7 (ESD), and that its immunity to radio frequency interference is not compromised. Ensure that the Kryo550-16 is not running a program and that the mains power is disconnected before connecting or disconnecting any cable. Fit the plastic covers and plugs provided to all unused connectors.
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Page 8: Table Of Contents
Chapter 1 Introduction to the Kryo550-16 ........ - Page 9 Contents vii Safety Declaration ........... . .
- Page 10 viii Contents...
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Page 11: Chapter 1 Introduction To The Kryo550-16
Chapter 1 Introduction to the Kryo550-16 Welcome to the Kryo550-16, a fully featured tissue bank freezer for the cryopreservation of bone marrow, stem cells, skin, cord blood and other critical high volume samples. This manual is intended for both operators of the freezer and those persons responsible for its installation. -
Page 12: Typical Installation
1-2 Introduction to the Kryo550-16 Typical Installation Mains power Pump power supply LNP4 pump and dewar Printer Personal computer Ethernet hub Alternative network connection if kryo-connect card fitted... -
Page 13: Control Panel
Introduction to the Kryo550-16 1-3 Control Panel Display Normally shows chamber temperature. While the Sample key is pressed, the display shows the sample temperature. Run indicator light Illuminated when running a profile. Run button Press to start a profile Flashes for operator attention. -
Page 14: Interface Panel
1-4 Introduction to the Kryo550-16 Interface Panel Reset button Manual override - heat Manual override - cool Sample PRT Alarm output RS232 Serial port kryo-connect ethernet connector (if fitted) -
Page 15: Rear Panel
Introduction to the Kryo550-16 1-5 Rear Panel Mains on/off switch Pressure relief valve Mains power inlet Liquid nitrogen inlet... -
Page 16: Kryo550-16 Features
All results from the run are stored on the PC for traceability purposes. Any profile you run from the PC may be selected to be stored in the Kryo550-16's memory (overwriting any previously stored profile). This enables you to rerun the profile directly from the freezer without having the PC connected. -
Page 17: Liquid Nitrogen Delivery System
Kryo550-16 Profile Memory The Kryo550-16 has two memory locations that are used for storing profiles. These are the Permanent Profile and Run Profile areas. When the Run button on the control panel is used to start a profile, the freezing profile stored in the Permanent Profile memory is copied into the Run Profile location and then started. - Page 18 1-8 Introduction to the Kryo550-16...
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Page 19: Chapter 2 Installing The Kryo550-16
If you do not have the kryo-connect option and want to interface with a PC, connect RS232 serial port connector ( 9 way D type plug) on the side of the Kryo550-16 to an RS232 port on the PC using the cable supplied. This is a straight-through one to one cable. -
Page 20: Installing A Cryocyl Low Pressure Cylinder
The liquid-nitrogen supply pressure must not exceed 2.0 bar (30 psi). If the hose is not already fitted to the Kryo550-16, wrap the male hose thread with PTFE tap and fix to the LN input on the back of the chamber. Tighten securely with a spanner. -
Page 21: Installing A Planer Pump And Dewar
To avoid damage to the heating element, support the pump carefully while it is out of the dewar. If the hose is not already fitted to the Kryo550-16, wrap the male hose thread with PTFE tap and fix to the LN input on the back of the chamber. - Page 22 2-4 Installing the Kryo550-16 Fitting the pump Wear protective clothing, including cryogloves, goggles, boots and a protective apron. Ensure that the red pressure-release valve on the pump is open. Ensure that power is disconnected from the pump. Ensure the delivery hose from the pump to the chamber is securely tightened.
- Page 23 Installing the Kryo550-16 2-5 Testing the Dewar Wear protective clothing, including cryogloves, goggles, boots and a protective apron. Press the switch on the pump power supply unit. The adjacent light will illuminate. After about 5 minutes, the pressure will rise to 5 psi and the light will switch off. The dewar should remain pressurised until almost empty.
- Page 24 2-6 Installing the Kryo550-16...
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Page 25: Chapter 3 Freezing Samples
It is normal to load the samples after the chamber has reached the start temperature. If you intend to run from a PC, initiate the profile from the PC using the Planer plc application - DeltaT. Refer to the DeltaT documentation for details. -
Page 26: Removing The Samples
Run button on the control panel. This will cause the Kryo550-16 to start heating to its start temperature. Once the Kryo550-16 starts beeping to indicate that the start temperature has been reached, the Stop button can be pressed to end the run. -
Page 27: About Seeding
When the seeding start temperature is reached, the chamber temperature is maintained at that temperature for the specified soak time. After this period, the Kryo550-16 displays S and beeps. The seed temperature is still maintained, but the operator will normally momentarily remove the samples and seed manually;... - Page 28 3-4 Freezing Samples Pulse seeding Long pulse durations can cause the chamber to overcool so this feature should be used with care. When determining the appropriate time always start with a very short duration, less than 5 seconds, and then experiment with longer times until an appropriate duration has been found.
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Page 29: Power Failures
When you have removed the samples, press the reset button on interface panel . To deal with a system failure Switch off the power to the Kryo550-16 using the mains on/off switch at the rear. Reset the Kryo550-16’s microprocessor. The reset button is operated by inserting the tip of a ball-point pen or similar tool through the hole in the interface panel located just above the manual override buttons. - Page 30 3-6 Freezing Samples...
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Page 31: Chapter 4 Routine Maintenance And Troubleshooting
Check all mains cables and interconnecting cables for signs of damage The Kryo550-16 is classified as Class I equipment and must be earthed for safe operation The freezer, pump and the mains connecting cords should be regularly checked by suitably trained personnel, using a Portable Appliance Tester or similar equipment, to ensure adequate earth bonding. -
Page 32: Troubleshooting
4-2 Routine Maintenance and Troubleshooting Troubleshooting Refer to the safety instructions at the front of this manual. The Chamber will not Cool Check the nitrogen supply. Check that the pump filter is not blocked by ice (if using an LNP4 pump). The Chamber will not Heat Check that the freezer did not overheat (wait until the thermal cut-out resets). -
Page 33: Returning For Service
Routine Maintenance and Troubleshooting 4-3 Returning for Service Should the system or any part of the system need to be sent back to Planer plc for repair, or if the system is to be inspected, maintained or repaired on-site by Planer plc personnel, the Safety Declaration described in Appendix C - Safety Declaration must be completed. - Page 34 4-4 Routine Maintenance and Troubleshooting...
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Page 35: Appendix A Specifications
Specifications A-1 Appendix A Specifications Kryo550-16 System Specifications Operation Temperature range +30 ºC to -180 ºC Heating rates 0.01 ºC/minute to 10 ºC/minute Cooling rates -0.01 ºC/minute to -50 ºC/minute Programmable cooling -0.01 ºC/minute to -99 ºC/minute rates Controller accuracy ±... -
Page 36: Fuses
Note. The Kryo550-16 systems are designed to be connected to an Installation Category (over-voltage category) II mains supply as defined in IEC 664. Fuses There are no user replaceable fuses. The Kryo550-16 mains inlet is fitted with a circuit breaker to IEC/EN 60934, UL 1077. Freezer type Nominal trip at +23 ºC... -
Page 37: Appendix B Alarm Output
Appendix B Alarm Output Alarm Output The Kryo550-16 is provided with an alarm output at the side of the interface panel. This is a normally closed voltage-free relay output. Only connect the alarm output to equipment that complies with EN 61010-1 or IEC 950 or their equivalent. - Page 38 B-2 Alarm Output...
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Page 39: Appendix C Safety Declaration
The declaration overleaf must be completed, signed by a competent authority and returned to Planer - before the service engineer arrives at the laboratory ( or before equipment is received at factory when returning for repair ). - Page 40 I declare that I have taken all reasonable steps to ensure the accuracy of the above information, in accordance with HSG(93)26. Authorised Unit signature _______________ _______________ Name (printed) Dept _______________ _______________ Position Tel no. _______________ _______________ Date Fax No. _______________ _______________ This page may be photocopied for use as a declaration for the Kryo550-16 freezer.
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