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Table of Contents
Troubleshooting
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Summary of Contents for Planer Kryo250
- Page 1 Kryo250 Operators' Manual...
- Page 2 © 2004 Planer plc. Planer plc 110 Windmill Road Sunbury-on-Thames Middlesex U.K. TW16 7HD Telephone +44 (0) 1932 755000 Facsimile +44 (0) 1932 755001 Kryo 250 Operators’ Manual MA009478 Issue 1.0 July 2004...
- Page 3 Hard copies and backup copies should be routinely kept. All equipment supplied by Planer plc. is subject to a Warranty Agreement and Limited Liability. In addition, software products are not sold but are licensed for use and are subject to a Licence Agreement.
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Page 4: Symbols Used In This Manual
Symbols Used in this Manual Failure to follow these instructions could result in personal injury. Failure to follow these instructions could result in damage to equipment or samples. This indicates first-aid advice. Symbols Used on the Equipment Caution: Refer to this manual and accompanying documentation Mains supply ON Mains supply OFF... -
Page 5: Safety Precautions
SAFETY PRECAUTIONS IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO OPERATING ANY EQUIPMENT First Aid IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any quantity of liquid nitrogen comes into contact with the skin or eyes, immediately flood that area of the body with large quantities of unheated water, apply cold compresses and seek medical attention. - Page 6 Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available as an accessory from Planer plc. When running a program, the chamber and contents may get cold enough to cause frostbite. The dewar must be depressurised before the pump can be removed. This is achieved by opening the pressure-relief valve mounted on the side of the pump, which seals an orifice in the pressure system by toggle action.
- Page 7 The mains outlets on the chamber’s rear panel are only for use with the Planer LNP4 unit and the Kryo250 Controller. The 9-way plug on the Controller is only for connecting to the RS232 serial port of a computer that complies with IEC950.
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Page 8: Emc And Esd Considerations
EMC AND ESD CONSIDERATIONS The following precautions must be taken to ensure that the equipment is not damaged by electrostatic discharge (ESD), and that its immunity to radio frequency interference is not compromised. Ensure that the Controller is not running a program and that the mains power is disconnected before connecting or disconnecting any cable. -
Page 9: Table Of Contents
Rear Connections ..... . . Installing the Kryo250 ...... - Page 10 viii The Chamber will not Cool ....The Chamber will not Heat ....No Response from the System .
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Page 11: Installing The System
Installing the System 1 - 1 Chapter 1 Installing the System Overview of the System An overview of the system and connections is given in the following diagrams. Top View Mains power inlet Power outlet for LNP4 pump Power to 37 way Sample PRT socket controller... -
Page 12: Rear Connections
1 - 2 Installing the System Rear Connections Rear panel of chamber Rear panel of controller 37 way D Type connection to chamber Sample PRT RS232 to PC AUX socket Reset button Manual override Liquid Power from nitrogen chamber to inlet controller Power to LNP4 pump... -
Page 13: Installing The Kryo250
Installing the System 1 - 3 Installing the Kryo250 Carefully unpack the equipment. Place the Kryo250 Controller and the Chamber on a secure, flat surface. Connect the 37 way D Type socket from the Chamber to the plug on the rear of the Controller. -
Page 14: Installing A Cryocyl Low Pressure Cylinder
The liquid-nitrogen supply pressure must not exceed 1.7 bar (25 psi). The Controller is optimised for use with a Planer pump and dewar ruuning at approximately 0.5 bar (7.5 psi). If running from a 1.5 bar (22 psi) cylinder, alternative control coefficients should be used. -
Page 15: Installing A Planer Pump And Dewar
Installing the System 1 - 5 Installing a Planer Pump and Dewar Connecting the Delivery Hose Never insert the pump into a dewar until it has been connected to the chamber. To avoid damage to the heating element, support the pump carefully while it is out of the dewar. -
Page 16: Fitting The Pump
Close the pressure-relief valve. The mains power outlet on the rear of the Chamber must only be used for connection to a Planer Products pump. Connect the mains inlet of the pump power supply unit to the auxiliary power output on the rear of the Chamber. -
Page 17: Testing The Dewar
The dewar should remain pressurised until almost empty. Before rectifying any leaks, or to shutdown the system, switch off the Kryo250 system, disconnect the mains supply at the wall socket, open the red pressure-relief valve and wait for the flow of gas to stop. - Page 18 1 - 8 Installing the System Press firmly on the upper surface of the pump, then remove the spring clips from the handles on the dewar. To prevent jamming only remove one spring at a time from each side. Lift the pump carefully, but firmly, out of the neck of the dewar using a slight twisting and rocking action.
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Page 19: Operating The Kryo250
Operating the Kryo250 2 - 1 Chapter 2 Operating the Kryo250 Chamber Front Panel Indicates when Indicates when Mains on off chamber is being chamber is being switch cooled heated... -
Page 20: Controller Front Panel
2 - 2 Operating the Kryo250 Controller Front Panel Chamber temperature (or sample temperature while sample button is depressed). EEEE indicates that the chamber or sample PRT is faulty. A profile -120.4 can be run without a working sample PRT but the chamber PRT must be in full working order. -
Page 21: Controller Rear Panel
Operating the Kryo250 2 - 3 Controller Rear Panel 37 Way D Type Connector for Chamber Connection RS232 Connection to PC Auxilliary connector for service calibration only Reset the controller Press to force a reduction in temperature Press to force an increase in... -
Page 22: Freezing Samples
If you are using a CryoCyl cylinder, make sure that the liquid-nitrogen supply tap is open. v If you are using a newly-filled Planer dewar and pump, switch on the pump power supply, then wait for the orange light to switch off; this indicates that the correct pressure has been attained. -
Page 23: Loading The Samples
Operating the Kryo250 2 - 5 Loading the Samples Always use tongs or wear cryogloves when handling cold objects. v Load the samples. This will depend on the freezer furniture fitted to your Chamber. v After loading all of the samples, it is advisable to wait 5 minutes to allow the chamber to re-stabilise. -
Page 24: Shutting Down The System
. (see Power Failures below ) If you have finished using the system for a short period of time, simply remove all specimens from the chamber and switch off the Kryo250 and pump when the chamber is back at ambient temperature. - Page 25 Operating the Kryo250 2 - 7 During this time, the solenoid and heater will not operate, but the controller will continue to monitor the chamber temperatures to ensure that it can resume the profile upon return of mains power. If power resumes within 1 minute, the run will continue. If power is removed for...
- Page 26 2 - 8 Operating the Kryo250...
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Page 27: Routine Maintenance And Troubleshooting
The Kryo250 System is classified as Class I equipment and must be earthed for safe operation v The Kryo250 System and the mains connecting cord should be regularly checked by suitably trained personnel, using a Portable Appliance Tester or similar equipment, to ensure adequate earth bonding. -
Page 28: Calibrating The System
3 - 2 Routine Maintenance and Trouble Shooting Calibrating the System The system will have been factory calibrated when first supplied. However, the system should be regularly calibrated to ensure its continued accuracy. Contact your local distributor for details on calibrating the system. Troubleshooting The Run has Stopped Prematurely A run could stop prematurely if, for example, the liquid-nitrogen supply is used... -
Page 29: The Chamber Will Not Cool
The Chamber will not Cool v Check the nitrogen supply. v Check the fuses. v Check that the pump filter is not blocked by ice (if using a Planer pump). The Chamber will not Heat v Check the fuses. v Check that the Chamber did not overheat (wait until the thermal cut-out resets). -
Page 30: Returning For Service
3 - 4 Routine Maintenance and Trouble Shooting Returning for Service Should the system or any part of the system need to be sent back to Planer plc for repair, or if the system is to be inspected, maintained or repaired on-site by Planer plc personnel, the Safety Declaration described in Appendix B - Safety Declaration must be completed. -
Page 31: Appendix A Specifications
Specifications A - 1 Appendix A Specifications System Specifications Accuracy ± (0.3 + 0.005 x TM)ºC (where TM is the magnitude of the temperature). Storage temperature -10ºC to +50ºC. Storage humidity 5% to 95% relative humidity non-condensing. Operating environment For indoor use only Operating temperature 5ºC to +40ºC. -
Page 32: Chamber Specifications
200 x Ø150 0.25ml straws 0.5ml straws 2ml ampoules Liquid nitrogen 0.34 to 0.52 bar (5 to 7.5 psi) from Planer pump and dewar supply 1.5 bar (22 psi) ± 10% from LN cylinder Power 115V ~ Requirements... -
Page 33: Appendix B Safety Declaration
The declaration overleaf must be completed, signed by a competent authority and returned to Planer - before the service engineer arrives at the laboratory ( or before equipment is received at factory when returning for repair ). - Page 34 B - 2 Safety Declaration PLANER plc SAFETY DECLARATION The preceding notes on page B-1 of the manual must be read prior to completing this form Service Customer Order Order No. Equipment Serial Type Nos. The equipment listed above has been cleaned as necessary to render it safe to handle and service.
- Page 36 MA009478 Kryo250 Operators’ Manual...
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