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Leica Microsystems Visoria B Instructions For Use Manual

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From Eye to Insight
Visoria B
Instructions for Use

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  • Page 1 From Eye to Insight Visoria B Instructions for Use...
  • Page 2 (in whole or in part) by print, photocopy, microfilm or other method (including electronic sys- tems) is not allowed without express written permis- sion from Leica Microsystems CMS GmbH. The instructions contained in the following documen- tation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible.

  • Page 3: Table Of Contents

    Notes on Handling Immersion Oil Köhler Illumination Notes on Handling Acids and Bases Checking Phase Contrast Rings* Disposal Adjustment of Condenser Prisms The Visoria B Operation Overview of the Instrument Switching on Main Components of the Instrument Stages and Object Displacement...
  • Page 4 8.4.6 Correction for Vision Problems Retrofitting Components Objectives 14.1 Fitting the Filter Magazine * (Transmitted Light) 8.5.1 Changing Objectives 14.2 Equipping the Condenser Disk * 8.5.2 Immersion Objectives Front Control Panel EU Declaration of Conformity 8.6.1 Incident Light Illumination (IL) 8.6.2 Transmitted Light Illumination (TL) 8.6.3 Fluorescent Light Illumination (R/G/B/UV) 37 8.6.4 Image Capture...

  • Page 5: Important Notes About This Manual

    Therefore, it must be kept and taken care of. Unless specified explicitly, all information in this man- Warning of optical radiation! Never ual is applicable to Visoria B. look directly into the light beam! Wear safety goggles! Text Symbols, Pictograms...
  • Page 6 Important Notes about this Manual UK Responsible Person Leica Microsystems (UK) Limited Larch House, Woodlands Business Park, Milton Keynes, England, United Kingdom, MK14 6FG...

  • Page 7: Intended Purpose Of Microscopes

    Intended Purpose of Microscopes Intended Purpose of Microscopes Reasonably Foreseeable Misuse The Visoria B microscopes are upright, light micro- scopes and are intended for use as general lab micro- The following are prohibited: scopes for routine examinations of biological speci- •...
  • Page 8 For use of the microscope with lasers, Leica Micro- systems offers special microscope variants with ad- ditional safety features. Laser couplings require cor- responding safety devices that have to be inspected and installed by trained personnel. For further information, please contact your autho- rized Leica Microsystems representative.

  • Page 9: Safety Notes

    H use – EMC requirements – Particular requirements 1.6 A, slow-blowing – In vitro diagnostic (IVD) medical equipment. Note: For Visoria B, the group 1 class B limits, the Safety Instructions measuring methods and the provisions given 3.3.1 Dangers Associated with in CISPR 11 Subclause 6.2 apply.

  • Page 10: Fuses

    Safety Notes Notes on Handling Light For connections without a ground connector, Leica Service must be consulted. Sources • Use only original power cables or alternative cables • Light sources pose a potential irradiation risk with a VDE / HAR logo, which at least fulfill the re- (glare, UV radiation, IR radiation).

  • Page 11: The Visoria B

    The Visoria B The Visoria B Overview of the Instrument Specification Visoria B Contrast Method • Transmitted Light: à Brightfield, Darkfield, Phase Contrast, Polarization, Differential Interference Contrast • Incident Light: à Fluorescence Transmitted Light Axis LED lamp housing Manual adjustment of: •...
  • Page 12 • UCL 0.90/1.25 OIL universal condenser (UCLP 0.85 for polarization with 5-position light ring disk) (Visoria B with objectives < 10× require the adapter lens (diffuser)) • UCL/P pol. universal condenser with interchangeable condenser head and condenser disk with 6 positions Focusing •...

  • Page 13: Main Components Of The Instrument

    The Visoria B Main Components of the Instrument Fig. 1 Left and right side view of Visoria B Fluorescent LED lamp housing 13 Condenser Condenser height adjustment 14 Objective prism slide On/Off switch (see also Fig. 28.1) 15 Eyepiece tube...

  • Page 14: Dimensions

    The Visoria B Dimensions Fig. 2 Dimensions...

  • Page 15: Unpacking The Microscope

    Unpacking the Microscope Unpacking the Microscope CAUTION CAUTION Do not yet connect the microscope and pe- When installing the microscope, make sure ripherals to the power supply at this point! the power inlet is freely accessible so that the instrument can be quickly disconnected from the mains if necessary.

  • Page 16: Assembling The Microscope

    Assembling the Microscope Assembling the Microscope 6.1.1 Specimen Holder The microscope components are logically assembled in this order: Place the specimen holder on the stage and fasten • Stage it with the two screws (Fig. 3.1). • Condenser • Fluorescence illuminator* •...

  • Page 17: Stage Lock

    Assembling the Microscope 6.1.3 Stage Lock* Loosen the lock screw (Fig. 5.1) at the front lefthand side of the stage. The stage lock is mounted in the same hole as the stage drive (in case of ErgoStages, it can be mounted on the opposite position in addition to stage drive).

  • Page 18: Tube And Eyepieces

    Fig. 9 Underside of condenser (example CL/PH) Orientation pin Auxiliary condenser lens LS (for Visoria B) Pull the condenser’s clamping screw (Fig. 8.2) so that the condenser is locked in place. NOTICE The condenser must be centered before using the microscope.

  • Page 19: Tablet And Tablet Holder

    Assembling the Microscope Tablet and Tablet Holder Usage of the tablet and tablet holder is only possible in combination with the Leica Flex- acam i5 camera. Take off the filter turret cover. Mount the Leica Flexacam i5 on the illumination axis (e.g.

  • Page 20: Objectives

    475/30 nm • G channel: 546/75 nm • R channel: 628/18 nm The Visoria B is equipped with the external LED lamp housing LH113 (order no. 11 504 199). The service life of the LED is about 25.000 hours. It cannot be replaced.

  • Page 21: Components For Fluorescence Applications

    Assembling the Microscope Components for The Visoria B may be equipped with a fluorescent LED light source (Fig. 15.1). This light source can be at- Fluorescence Applications* tached to the illumination axis by tightening a grub- 6.7.1 Fluorescence Illuminator* screw securing it in the port of the axis.

  • Page 22: Fluorescent Lamp Housing

    Assembling the Microscope 6.7.2 Fluorescent Lamp Housing Equipping the Fluorescence Turret Disk WARNING Risk of eye injuries Light sources pose a potential irradiation risk (glare, UV-radiation, IR-radiation). Therefore, lamps have to be operated in closed hous- ings and only after being mounted. Fig.

  • Page 23: Analyzer* And Polarizer* Analyzer

    Assembling the Microscope Analyzer* and Polarizer* Apply the included adhesive labels (Fig. 20.3) cor- responding to the installed parts to the front of Analyzer the fluorescence illuminator. 6.8.1 Analyzer When all filters and reflector cubes have been inserted, close the front cover plate again. Ensure that the cover snaps into place.

  • Page 24: Polarizer

    Assembling the Microscope 6.8.2 Polarizer Ergomodule* For raising the eye level of the tube opening, the Attach the polarizer holder to the underside of 30  mm, 60  mm or the variable ergomodule may be the condenser holder with the left clamp screw (Fig.
  • Page 25 Assembling the Microscope Leica DM Multiple Viewing Systems* Viewing attachments featuring illuminated pointers are available for groups of up to 10 viewers. The sup- port (Fig. 26.3) must be aligned precisely. The fade-in arrow can be moved in x and y direction. Fig.

  • Page 26: Start Up

    (Axis status). A separate connec- tion is provided for users of Visoria who want to ben- efit from fluorescent LED illumination (FL). Fig. 28 Connectors of Visoria B On/off switch IL – port for connecting incident light source (white light LED) (*) FL –...

  • Page 27: Köhler Illumination

    (Fig. 30c), the condenser must be moved into the middle of the field of view with the help of the two centering bolts (Fig. 29.6). The Fig. 29 Controls on the Visoria B tool required for this purpose is magnetically at- Condenser height adjuster tached to the underside of the stage.
  • Page 28 Start Up When swiveling in a suitable objective for phase contrast, the corresponding light ring must be selected. The objective engraving (e.g. PH 1) indicates the corresponding light ring (e.g. 1). In the place of an eyepiece, insert the focusing telescope (Fig.

  • Page 29: Adjustment Of Condenser Prisms

    Start Up Adjustment of Condenser Insert the centering screws (Fig. 33.1) into the openings provided at the rear of the condenser. Prisms If the equipment was delivered assembled, the con- denser prisms will already have been adjusted at the factory, but it is advisable to check the adjustment from time to time, especially after shipping.
  • Page 30 Start Up Engage the condenser-side prisms one af- ter the other and focus the telescope on dark diagonal compensation stripe (Fig. 35) by slightly loosening the clamping ring (Fig. 31.2) and moving the eye lens (ig. 31.1). The compensator must be inactive, i.e. the engrav- ing λ...

  • Page 31: Operation

    (Fig. 37.b.1) downward; repeat with the upper grip (Fig. 37.b.2). Fig. 36 Controls on the Visoria B Stage lock screw Coaxial pinion mounting screw Stage positioning Fine focusing Fig. 37 Standard coaxial pinion, b coaxial pinion with...

  • Page 32: Focusing

    Operation Right-/Left-Hand Operation After installation of the stage control, move object guide all the way to the left side of the instrument. The coaxial pinion can be attached to either side Keep turning when guide has reached the end of of the stage.

  • Page 33: Speed Switch (Optional)

    Operation 8.3.2 Speed Switch (optional)* 8.3.4 Setting the Torque Fine focusing can be adjusted in two speeds. To The torque of the focus drive is preset in factory. It switch speeds, press the left focus wheel to the right can be adjusted using the knurled wheel at the right- hand focus knob (Fig.

  • Page 34: Tubes

    Operation Tubes Fig. 40 Tube range HC L Binocular observation tube HC LB 0/3/4 Photo adapter, with 2 exits (50 % / 50 %) Ergonomy tube HC LVB 0/4/4, binocular, viewing Photo TV exit angle 0-35° additional ergotube (short) Other tubes on request. Some viewing tubes will be HC -/0/4, swivelable 7.5°-32.5°...

  • Page 35: Adjusting The Viewing Distance

    Operation 8.4.1 Adjusting the Viewing Adjusting the Eyepiece Section to the Arm Length Distance On the AET22 tube, the eyepieces can be extended Adjust the viewing distances of the eye-pieces so up to 30 mm (Fig. 41). that a congruent total image is seen (Fig. 41). 8.4.3 Beam Splitting in Photo Tubes EDT22 Tube The beam splitting between the observation and...

  • Page 36: Eyepieces With Inlaid Reticle

    DIN/ISO standards, cleaning see Chap- 8.5.1 Changing Objectives ter “Cleaning“ on page 53 In Visoria B, the objectives are manually swung into • W: Water immersion the beam path. • IMM: Universal objective for water, glycerol, oil...

  • Page 37: Front Control Panel

    Operation Front Control Panel 8.6.1 Incident Light Illumination (IL) White light LED source (LH113) may be attached to the back of the IL illumination axis by a grub screw. Its power cable must be connected to a connector marked IL on back plate of the microscope to enable the control of this light source with an associated IL button.

  • Page 38: Image Capture

    Encoding 8.8.1 Transmitted or Incident Light, Some components of a Visoria B microsope are en- Including Fluorescence coded for improved user efficiency while operating Adjust the brightness with the dial (Fig. 44).

  • Page 39: Aperture Diaphragm

    Operation Aperture Diaphragm 8.9.1 Color-Coded Condenser The color markings on the condenser (Fig. 46.2) cor- respond to the color rings of the objectives. When changing objectives, a suitable aperture diaphragm setting can be found by setting it to the matching color marking (corresponds to 2/3 of the objective-side aperture).

  • Page 40: Contrast Methods Transmitted Light

    When using a diffuser slider*, 2.5× magnification is also possible. Magnifications of 1.6× and 2.5× are also possible The Leica Visoria B with objectives <10× re- with the CL/PH or CLP/PH, UCL or UCLP condensers quires the diffuser slider*. if the condenser is removed completely. The field dia-...

  • Page 41: Brightfield

    Contrast Methods 9.1.1 Brightfield Bring the image into focus using the focus dial and set the brightness. DLF filter magazine for For an optimal field diaphragm setting, check the attachment to micro- Köhler illumination (Chapter 7.3 on page 27). scope base Use suitable transmitted light filters as applicable (except Leica DM2500) (Fig.

  • Page 42: Darkfield

    Contrast Methods 9.1.3 Darkfield Special darkfield condensers are available (Fig. 48). The application potential of the DF condensers de- pends on the aperture of the objective in use. For objectives with a built-in iris diaphragm, the aperture can be adapted. DF Condenser Max.
  • Page 43 Contrast Methods Rotate the polarizer until you observe the maxi- mum extinction position in the eyepiece (Fig. 51). Fix the cross position thus determined with the clamping screw. Crossing the polarizers when observing through a fo- cusing telescope or Bertrand lens, high-aperture Pol objective: Fig.

  • Page 44: Differential Interference Contrast

    Contrast Methods 9.1.6 Differential Interference Contrast* Insert a specimen, rotate a suitable objective into place and bring the image into focus. Turn the disk in the UCA/P condenser to the brightfield position. If present: switch the fluorescence illuminator into an empty position or filter system A. Pull the objective prism slide out of the tube slit.

  • Page 45: Fluorescence

    Contrast Methods Fluorescence* Insert a suitable specimen and rotate an appropri- ate objective into place. Focus the image initially in transmitted light if ap- propriate. Select an appropriate excitation wavelength of the fluorescent LED light source by pressing a but- ton on the control panel.

  • Page 46: Measurements With The Microscope

    Measurements with the Microscope Measurements with the Microscope 10.1 Linear Measurements • Remember to take the additional mag- nification value into consideration! We The following are required for linear measurements: strongly recommend you to calibrate each • Graticule with scale division* in eyepiece or a digi- objective separately instead of extrapolat- tal linear measuring eyepiece*.

  • Page 47: Thickness Measurements

    Measurements with the Microscope 10.2 Thickness Measurements Example: The upper and lower surfaces of a thin polished specimen have been focused with a dry objective = 1.0), scale readings of the mechanical fine drive (division spacing = 1 µm): 9.0 and 27.0. Therefore d‘...
  • Page 48 Measurements with the Microscope Orienting the lambda plate compensator* The following section explains the basic procedure for gout/pseudo gout differentiation. This test is made possible due to the negative birefringence of urates and positive birefringence of pyrophosphates. Both gout (monosodium urate) and pseudo gout (calcium pyrophosphate) crystals tend to be needle shaped.
  • Page 49 Measurements with the Microscope Procedure The following is the procedure for identification of pseudo gout: To insure the test is being done correctly, a slide of The test for pseudo gout is done identically to the test known monosodium urate crystals should be used for gout.

  • Page 50: Troubleshooting

    Troubleshooting Troubleshooting Problem Cause/Remedy Stand The microscope does not respond. Make sure that the stand is connected to the power supply. Check the cable connections. Check whether the fuse is defective and replace it if neces- sary (see Chapter “Changing Fuses“ on page 54). Illumination The image is completely dark.

  • Page 51: Transmitted Light Interference Contrast

    Troubleshooting Problem Cause/Remedy Phase contrast No phase contrast is possible. The specimen is too thick, too thin or too brightly stained. Refractive indiceses of the mounting medium and speci- men are identical, so that there is no phase jump. The cover glass is not placed planar. Check the right light ring (see Chapter “Checking Phase Contrast Rings*“...
  • Page 52 Troubleshooting Problem Cause/Remedy Fluorescence The image is completely dark (no fluores- Open the shutter (see Chapter “Fluorescence*“ on cence). page 45). Check the antigen-antibody combination. Insert a new lamp (see Chapter “Control of Fluorescent Light Source for the Incident Light Axis“ on page 20). The fluorescence is too weak.

  • Page 53: Care Of The Microscope

    Care of the Microscope Care of the Microscope Cleaning Coated Parts WARNING Risk of electrical shock Dust and loose dirt particles can be removed with a Unplug the power supply before per- soft brush or lint-free cotton cloth. forming cleaning and maintenance work! Clinging dirt can be cleaned with a little soapy water Protect electrical components from mois- or alcohol.

  • Page 54: Changing Fuses

    Care of the Microscope 12.4 Changing Fuses WARNING Risk of electrical shock Before exchanging the fuses or lamps, be absolutely certain to switch off the main power switch and remove the power cable. NOTICE Never use any fuses as replacements other than those of the types and the current ratings listed here.

  • Page 55: Essential Wear And Spare Parts

    APO objective ,10 ml 11 513 860 Type N, ISO 8036, low autofluorescence, 20 ml 11 513 861 Type N, ISO 8036, glow autofluorescence, 250 ml Fuses 13 302 138 946 910 T1,6A H, 250 VAC Fuse for Visoria B...

  • Page 56: Retrofitting Components

    Retrofitting Components Retrofitting Components 14.1 Fitting the Filter Magazine * 14.2 Equipping the Condenser (Transmitted Light) Disk * Fig. 59 Filter magazine (transmitted light) Remove the tube and intermediate systems where Fig. 60 Condenser UCL applicable. Fixing screw for condenser disk Turn the microscope stand upside down, loosen Turn the stage upward and lower the condenser.
  • Page 57 Retrofitting Components Condenser UCA/P Remove the screw (Fig. 60.1) completely. Turn back the centering screws (Fig. 61.3) until the light rings, λ and λ/4-compensator* and lens* 2.5× can be inserted. 3b 4 6a The largest hole is for brightfield observation (= BF), the slightly smaller ones for light rings or λ...
  • Page 58 Retrofitting Components The lettering of the components should correspond the marking at the opposite position (outer edge of the disk). Inserting DIC condenser prisms: Insert prisms K2, K3, etc into the large holes as fol- lows: Turn back the centering screws slightly. Prism labeling upward, the name K2 ,...

  • Page 59: Eu Declaration Of Conformity

    EU Declaration of Conformity EU Declaration of Conformity To download the EU Declaration of Conformity for your product use the following link: https://downloads.leica-microsystems.com/DM%20LA/Certificate/Leica_DM_LA_DM_LAM-EC_Declaration. Select the type of your microscope and switch to the Download page. 有害物质标记表 Hazardous Substance Marking Table 有害物质...
  • Page 60 CONNECT WITH US! Leica Microsystems CMS GmbH | Ernst-Leitz-Strasse 17–37 | 35578 Wetzlar (Germany) | Tel. +49 (0) 6441 29-0 | F +49 (0) 6441 29-2599 www.leica-microsystems.com...

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