Related Manuals for Leica Microsystems Mateo FL
Summary of Contents for Leica Microsystems Mateo FL
- Page 1 Mateo FL User Manual Leica Microsystems CMS GmbH, User Manual Mateo FL, 11934230, V00, 2024-05-22...
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Page 2: Table Of Contents
Hazardous Substance Marking Table Installing Object Guide and Holding Frame Introduction Installing Object Guide Intended Use Installing Holding Frames Directions for Use of Mateo FL RUO Getting Familiar with System Settings System Specifications Quick Save Image System Overview Image Format... - Page 3 Care and Maintenance Taking an Image Technical Data Adjusting Scale Bar Storage Multichannel Capture Zooming in/out in Live View Managing Images in Gallery Checking Image Parameters Changing Gallery Folder Path Selecting Image(s) Zooming in/Zooming out in Gallery Mateo FL User Manual...
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Page 4: Important Notes
Important Notes This user manual is an essential part of the product. It must be read carefully before the product is assembled, put into operation or used and must be kept for later reference. Important Notes Mateo FL User Manual... -
Page 5: Symbols Used
Serial number ● Product malfunctions and damage Warning of hazardous electrical voltage! Risk of electrical shock! Failure to comply may cause the following: ● Personal injury. ● Product malfunctions and damage. Warning of electromagnetic field Mateo FL User Manual Important Notes... -
Page 6: Safety Notes
Do not use damaged, non-functioning components or accessories. Instead, notify your Leica branch office or Leica dealer. IMPORTANT! Mateo FL should not be subjected to UV sterilization. UV degrades many materials, including plastic. Damage In order to ensure the product reliability and warranty services,... -
Page 7: Directives Of European Community (Ec-Directives)
IEC 62368-1: Information technology equipment – Safety – Part one: general requirements The Mateo FL microscope has also been tested in accordance with EN Radio interference suppression in compliance with EN 55011 62471/IEC 62471 photobiological safety of lamps and lamp systems and class B is classified in risk group 1 (low risk). -
Page 8: Electrical Safety
Do not repair the power supply. Electrical work must only be carried out by Leica Service. To avoid injury to the user and for cooling reasons and fire protection, never remove the covers of the components. Important Notes Mateo FL User Manual... -
Page 9: Photobiological Safety
Please contact Leica Service in case of high condensation. High condensation can harm the device. The light source of Mateo FL generates high-energy light with The microscope’s electrical accessory components are not invisible UV components. Always use the provided protective shield. -
Page 10: Notes On Handling Acids And Bases
GB/T 26572. x: Indicates that said hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement of GB/T 26572. Important Notes Mateo FL User Manual... -
Page 11: Introduction
Introduction The Mateo FL is a fluorescence digital inverted microscope and is intended for use as a general laboratory microscope for routine examinations of biological samples such as cells and tissues. Mateo FL User Manual Introduction... -
Page 12: Intended Use
Intended Use Mateo FL is a digital inverted fluorescent light microscope for routine quick cell check, providing user coding function/all-in-one PC and software features including label-free counting as well as confluency and transfection efficiency to increase the ease of use. -
Page 13: System Specifications
USB ports 1x USB 3.0 and 3x USB 2.0 USB power output 5V, 0.5A (back plate) 5V, 1.0A (on the side of the stand) AI-based software modules Confluency module, cell counting module, transfection efficiency module Mateo FL User Manual Introduction... - Page 14 Overvoltage category: II Pollution degree: class II Microscope Voltage: 12 VDC Max. power: 84 W Manufacturer Leica Microsystems CMS GmbH, Ernst-Leitz-Strasse 17–37, 35578 Wetzlar (Germany) Tel. +49 (0) 6441 29-0, F +49 (0) 6441 29-2599 Mateo FL User Manual Introduction...
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Page 15: System Overview
Supports precise movement of the sample vessel. 4. Power/Stand-by button with LED indicator ● Indicator light on: indicates the system is on. ● Indicator light off: indicates the system is off. ● Indicator light blinks when the button is pressed. Mateo FL User Manual Introduction... -
Page 16: Rear View
2. Power switch Press to power on/off the system. 3. Supporting handle Hold the handle to facilitate safe and steady transportation of the equipment. 4. Ethernet connection 5. Power supply connection For plugging in the power supply. Mateo FL User Manual Introduction... -
Page 17: Left View
For fine tuning the focus of the specimen. 4. Nosepiece Nosepiece is the base where objectives are to be installed. Rotate the nosepiece to move the objective of interest to the light path. 5. Objective For magnifying the sample. Mateo FL User Manual Introduction... - Page 18 Right view 6. USB ports Left: USB 2.0 Right: USB 3.0 7. Condenser 8. Filter cube turret (under magnetic cover) Mateo FL User Manual Introduction...
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Page 19: Display In Upright And Folded Position (Dimension Data In Mm)
Display in Upright and Folded Position (dimension data in mm) 377 mm 611 mm 611 mm 397 mm 377 mm 466 mm 466 mm 397 mm Mateo FL User Manual Introduction... -
Page 20: Unpacking
Unpacking Before setting up Mateo FL, carefully remove all components from the transportation box and packaging materials. Unpacking Mateo FL User Manual... - Page 21 83. DAPI 390 Mateo FL base configuration: GFP ET The following parts will be included in the delivery: Y3 ET ● Mateo FL stand with integrated cameras and touch monitor Y5 ET ● UV protection cover ● Barcode reader ●...
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Page 22: Preparation
If you want to put the Mateo FL inside a laminar flow hood, you should check in advance whether there is a cable port inside the flow hood. -
Page 23: Connecting Power Supply
Check the LED status at the lower front side of your microscope: Steps: ● Indicator light on: indicates the system of Mateo FL is on. Connect the power cable and the power adaptor. ● Indicator light off: indicates the system of Mateo FL is off. -
Page 24: Turning On/Off The System With The Front Panel
2 seconds. When the system is in use, the light ring around the power button Mateo FL system comes with a built-in virtual keyboard. You can on the front panel is on, along with the monitor. -
Page 25: Installing Uv Protection Shield
"Transferring Image(s) to your Smart Device through Wi-Fi" on page 59. AI Software Modules For the usage of the corresponding module, see "Using AI-based Software Modules" on page 63. All software modules are already activated. Mateo FL User Manual Preparation... -
Page 26: Objective Setting
There are no pre-installed and pre-configured objectives before delivery. Screw the objective into the free position until it sits firmly. You can install up to 6 objectives in the nosepiece of Mateo FL, and switch to any one of them during experiment. The current objective in Avoid touching the lens surfaces of the objectives. -
Page 27: Configuring Objectives
Click the red arrow in the top bar to go back to the main screen. After the completion of one objective repeat the steps 4 and 5 to configure another objective. Please make sure the objective to be configured is positioned to the left of the nosepiece. Mateo FL User Manual Preparation... -
Page 28: Delete/Change The Objective Setting
Move your cursor to the icon of the objective (corresponding to by tilting the cover to the side. the numbering of the nosepiece) to be removed until a trash bin icon appears, or simply tap the objective you want to delete. Preparation Mateo FL User Manual... - Page 29 The filter cube sits firmly in its position. In the "Filter cube setting", enter the corresponding name and select the color for the filter cubes. The status is activated automatically when the configuration is completed. Mateo FL User Manual Preparation...
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Page 30: Installing Object Guide And Holding Frame
Align the two hexagon screws of the object guide with the two screw holes on the stage, as shown in the picture below. Turn the two screws counterclockwise manually until they are engaged with the holes. Preparation Mateo FL User Manual... -
Page 31: Installing Holding Frames
To exit from system "Settings" menu, click on the top right corner of the screen. To uninstall/remove the holding frame, you can pull it outward until it is off from the object guide. Mateo FL User Manual Preparation... -
Page 32: Quick Save Image
Enter the image name, and click "Confirm" to save the image. Then the gallery right after capturing an image, with the default naming rule system goes back to the main screen automatically. below: Prefix_contrast method_timestamp_extension Example: Leica_2021-12-12 120000.jpeg Preparation Mateo FL User Manual... -
Page 33: Image Format
"Quick save image" is disabled and you are manually naming a newly captured image. The images are stored chronologically to facilitate later reference and repeating experiment. (For details, see chapter "Repeating the Image Settings in the Gallery" on page 62). Mateo FL User Manual Preparation... -
Page 34: User Management
In the section "Overexposure indicator", select your preferred color from the drop-down menus for each of the channels. On Mateo FL you can create password-protected user profiles. Only the admin can access the user management system which allows them to reset, unlock and delete other user accounts and view the audit trails. - Page 35 Similarly, when deleting the last user profile, the admin account will be deleted automatically at the same time. User account name has to be unique in the system and cannot be: Admin, ADMIN, or Administrator. Mateo FL User Manual Preparation...
- Page 36 Alternatively, after restarting the device you will be prompted to create a contacted to reset it. password. As a user you can change your password by clicking "User" on the right panel. Only admins can reset passwords and unlock or delete user profiles. Preparation Mateo FL User Manual...
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Page 37: Audit Trail
To take a backup of the electronic record you can export the audit trail as a CSV file to a USB stick or to your network folder. No one can edit the Mateo FL creates an electronic record of user activities such as login, audit trail. -
Page 38: About System
Leica service. Click "Legal notice" to view the content. To update the software click on "Update from USB" and follow the instructions given in chapter "Updating Software" on page 71. Preparation Mateo FL User Manual... -
Page 39: Shading Correction For Phase Contrast
Place a uniform fluorescence slide on the stage. Lower the nosepiece using the focus knobs a few micrometers to get out of focus. Adjust mean brightness to green range by turning the light intensity adjustment wheel. Mateo FL User Manual Preparation... -
Page 40: Network Setting
, then "System" and "Network setting". Define the name and path of the network folder. If needed, contact your IT for support. Close the window by clicking Repeat steps 1 to 6 for each FL channel. Preparation Mateo FL User Manual... -
Page 41: Export Log File
Then, close the window by clicking Format requirement: ● Date: yyyy-mm-dd ● Time: hh:mm (24 hours) Select the desired folder and click "Confirm". If needed, provide the log file to Leica service for analysis. Mateo FL User Manual Preparation... -
Page 42: Language
Language Mateo FL supports English and Simplified Chinese. The default language is English. A system restart is required on each language switch. If the format requirement for date or time is not met, a warning message appears to remind you the date and time you entered is invalid. -
Page 43: Operations
Operations This chapter includes step-by-step instructions on how to use the system correctly. Please read the instructions carefully before operating the device. Mateo FL User Manual Operation... -
Page 44: Turning On The Microscope
Otherwise, you cannot proceed. Place the sample on the stage. Click "Camera" and select the correct camera type. For subsequent powering on/off, use the power button on the front panel of the device. Operation Mateo FL User Manual... -
Page 45: Phase Contrast/Brightfield
If the image quality does not reach your expectation, you can adjust the camera parameters for an optimum effect (see "Adjusting Camera Parameters" on page 45). With "Easy mode" disabled, you can individually adjust the parameters of the camera: Light Intensity, Exposure and Gain. Mateo FL User Manual Operation... -
Page 46: White Balancing
You can adjust each parameter through any way below: ● Move the slider by dragging it. ● Select the slider or input box, and adjust it by scrolling your mouse wheel. ● Enter value in the textbox. Operation Mateo FL User Manual... -
Page 47: Brightness Scaling
"Camera" setting Enabling or disabling "Brightness scaling" is only possible in live mode. The "Auto" function of "Brightness scaling" is enabled by default. You can disable it by clicking on the brightness scaling bar. Mateo FL User Manual Operation... -
Page 48: Fluorescence
To save an image manually with a preferred name, you need to disable the "Quick save image" by clicking to open the "Settings" menu and then clicking "Image" setting. (For details, please refer to "Quick Save Image" on page 24.) Operation Mateo FL User Manual... -
Page 49: Adjusting Scale Bar
"Settings" and "System". If it is due to shortage in storage space, please see "Storage" on page 87. When the user has live cells or non-adherent cells, to minimize vibration as much as possible, the user should use the mouse. Mateo FL User Manual Operation... - Page 50 You can also change its color and length. You can disable the scale bar by clicking "Scale bar" at the right panel of the screen. Click the scale bar at the bottom right corner of the screen. Operation Mateo FL User Manual...
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Page 51: Multichannel Capture
Checkboxes will appear next to each channel. Tick the desired checkboxes and click Capture to acquire a multichannel image. The scale bar length changes according to the objective magnification chosen and when zooming into the image. Mateo FL User Manual Operation... -
Page 52: Zooming In/Out In Live View
In the panel at the bottom of the screen you can see an overview of the images captured as well as an overlay image. Click "Save" to store the images in the gallery. They will be stored according to the image naming rule of the system. Operation Mateo FL User Manual... -
Page 53: Checking Image Parameters
If multiple images are selected, only the image that was clicked system is 500 GB. (surrounded with red borders) and its parameters are presented. (For To use the Network folder, see chapter "Network Setting" on page 40. details, see chapter "Selecting Image(s)" on page 54.) Mateo FL User Manual Operation... -
Page 54: Selecting Image(S)
Alternatively, you can click Filter and filter your images according to the date or other parameters. Click at the top left corner of the original image to close it. Operation Mateo FL User Manual... -
Page 55: Zooming In/Zooming Out In Gallery
You can move the window to the desired position on the screen. ● Click "Select all" at the top of the screen to select all images. Mateo FL User Manual Operation... -
Page 56: Using Measurement Tool
In the gallery select the image of interest, so that it is displayed on the right side of the screen. Click "Measure" to open the measurement tools. With these tools you can measure parameters such as area of regions of interest or distance between points of interest. Operation Mateo FL User Manual... -
Page 57: Deleting Image(S)
Select "Delete" from the drop-down menu and click "Delete" again to confirm your action. Method 3 (Renaming multiple images at once) Tick the checkbox of the image(s) of interest. Click "Rename" in the middle of the top panel. Rename the image(s) of interest. Mateo FL User Manual Operation... -
Page 58: Copying Image(S) To Usb Disk
You can undo this action to retrieve the deleted images by selecting the "Undo" button within 10 seconds before it disappears. This step is possible with each method. Operation Mateo FL User Manual... -
Page 59: Transferring Image(S) To Your Smart Device Through Wi-Fi
Follow the instructions in the prompted window to share your image(s). Please see "Table 2: Recommended USB disks and USB hard disks" on page 89. When the copy completes, a message appears: "Copy successful". Mateo FL User Manual Operation... - Page 60 APPs is not recommended to use due to compatibility issues. After a successful connection between your Mateo FL and your smart device, you can directly select the wireless network "Leica Wi- Fi+serial number" on your smart device without having to scan the QR code ‘1’...
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Page 61: Transferring Image(S) Through The Network Folder
In the gallery, select the image(s) of interest and click "Share" in the middle of the top panel. Select "Copy to network folder" and click your folder of interest. Mateo FL User Manual Operation... -
Page 62: Repeating The Image Settings In The Gallery
Gallery reference image. Many experiments are conducted repeatedly under the same conditions. With Mateo FL, you can take one image from the gallery as a reference and reuse its parameters to repeat the imaging conditions of the reference. Click to open the gallery where your experiment images are stored. -
Page 63: Using Ai-Based Software Modules
Many cell-based experiments require cell culture to reach a certain confluency. With Mateo FL, you can use the onboard Confluency module Click "OK, Start" to start repeating. - Page 64 If "Quick save image" is enabled, the image is automatically named with a suffix "-Confluency", following the naming rules of "Quick save image". Click "Calculate" to obtain the Confluency results. After that, the system closes the Confluency module and goes back to main menu. Operation Mateo FL User Manual...
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Page 65: Cell Counting Module
4 and 5 in chapter "Checking Confluency from Live Image" or the Auto button. on page 64. To save the image with the analysis, click "Save" or cancel to exit without saving. Click "Calculate" to obtain the cell counting results. Mateo FL User Manual Operation... - Page 66 You can also close the Cell Counting module manually by clicking "Close" in the right corner of the screen. To save the image with the analysis, click "Save" or cancel to exit without saving. Operation Mateo FL User Manual...
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Page 67: Transfection Module
Place the sample on the stage and focus the sample. to identify the cells in your image. To use the transfection module, you first need to acquire an image in PH and FL mode of the same field of view. Mateo FL User Manual Operation... - Page 68 "Cancel" to return to the Transfection module. The Transfection percentage is presented on the top right corner of the image. The cells in the image are outlined in yellow and the fluorescence signal is highlighted in pink. Operation Mateo FL User Manual...
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Page 69: Barcode Reader
You can also enter the information in the input box manually. To use the barcode function, plug in the barcode reader to a USB port of the device (wired: backport, wireless: sideport). Mateo FL User Manual Operation... - Page 70 You can see the barcode information in the image parameters section under "Others". If the input box is empty or the Barcode function is not activated, "N/A" will be displayed under "Others". Operation Mateo FL User Manual...
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Page 71: Updating Software
Updating Software You should update the software regularly to make sure the system is using the latest software version and keep your Mateo FL running at an optimum performance level. Mateo FL User Manual Updating Software... - Page 72 2. In the main screen, click to open the "Settings" menu. 3. Click "System" and "About system", you can see the current version of the software. 5. Click "Confirm" to proceed. Updating Software Mateo FL User Manual...
- Page 73 You can also click "Restart Now" to restart the system immediately. 8. If the update fails, it shows the failed result. Click "OK, Go Back" to roll back to the previous version. Mateo FL User Manual Updating Software...
- Page 74 9. Go back to "About system" to verify whether the software is the target version. It is also possible to downgrade the Mateo FL system to any previous version, if needed. To do so, you can download the package of your desired version and repeat the steps above.
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Page 75: Troubleshooting
Troubleshooting This chapter gives an overview about the most common problems and their possible causes and solutions. Mateo FL User Manual Troubleshooting... - Page 76 Possible cause: The wrong side of the slide is facing the objective. The image of samples on glass slide is blurry. Solution: The glass slide must be placed the right way. Possible cause: The sample/objective is not clean. Solution: Clean the sample/objective. Troubleshooting Mateo FL User Manual...
- Page 77 Possible cause: The USB disk is incompatible with the system. The system cannot detect the USB disk. Solution: Use one of the recommended USB disks (see "Table 2: Recommended USB disks and USB hard disks" on page 89). Mateo FL User Manual Troubleshooting...
- Page 78 Possible cause: The user used a built-in shortcut key function of the physical keyboard. (The used. shortcut key function is not supported in the Mateo FL system as the definitions of shortcut keys vary from manufacturer to manufacturer and they all cannot be verified.) Solution: Please reboot the system.
- Page 79 Possible cause: Signal from the sample is too low and auto brightness scaling is on. The image is noisy/grainy. Solution: Increase the light intensity under camera settings or by using the light intensity adjustment wheel. Turn off brightness scaling and adjust the parameters under camera settings. Mateo FL User Manual Troubleshooting...
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Page 80: Self-Diagnosis
Self-diagnosis Mateo FL features quick and easy self-diagnosis. With it, you can easily diagnose your system, get the needed technical information and report the issue to Leica service via your smart device. Self-diagnosis Mateo FL User Manual... - Page 81 "Settings" menu. Error message Then, click "Hardware" and "Start self-diagnosis" to start self- ● QR code (Login portal to Leica service website) diagnosing. Example result for normal system: You cannot stop self-diagnosing during the process. Mateo FL User Manual Self-diagnosis...
- Page 82 If the result shows a system with error message, scan the QR code to visit Leica service website and enter required information (model, serial number, etc.), then click "Submit Form" to send the information to Leica service. Self-diagnosis Mateo FL User Manual...
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Page 83: Care And Maintenance
Care and Maintenance The following chapter includes instructions on how to clean and protect the device to support the longlasting function of your Mateo FL. Mateo FL User Manual Care and Maintenance... - Page 84 "Cleaning of Microscope Optics". You can Do not clean any interior parts yourself. download the information from the Mateo FL product website. Please contact an authorized Leica dealer for technical service. In case of a leak or spillage, clean the surface of the filter cube chamber thoroughly before you open the magnetic cover.
- Page 85 You can also contact our Technical Service in case of any questions. with these chemicals. Maintenance, repair work and servicing Ensure that repairs are only carried out by Leica-trained service engineers. Only use original Leica spare parts. Mateo FL User Manual Care and Maintenance...
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Page 86: Technical Data
Technical Data This chapter gives an overview of onboard storage, recommended objectives and external devices such as keyboard, mouse, barcode scanner. Technical Data Mateo FL User Manual... -
Page 87: Storage
Example: than 2 GB. means you be done. cannot take an image. For the specific clearing method, please refer to chapter "Deleting Image(s)" on page 57. Mateo FL User Manual Technical Data... - Page 88 40x N PLAN L / CORR PH2 N PLAN L 3.3 – 1.9 0.55 11506298 40x HC PL FL L / CORR PH2 PL FLUOTAR L 3.3 – 1.9 0.60 11506203 63x N PLAN 0.26 0.80 11506184 Technical Data Mateo FL User Manual...
- Page 89 Table 3: Recommended smart devices Type Specification Apple iPhone iOS 15 and higher Apple iPad iOS 16 and higher SAMSUNG galaxy A52 Android 12 and higher SAMSUNG TAB S6 Lite Android 13 and higher Mateo FL User Manual Technical Data...
- Page 90 Microsoft Keyboard & Mouse: Wired Desktop 600 | Microsoft Accessories Table 6: Filter cubes Material No. Description 11504164 Filter system GFP ET, k 11504169 Filter system Y3 ET, k 11504171 Filter system Y5 ET, k 11533332 DAPI 390 Filter cube, size K Technical Data Mateo FL User Manual...
- Page 91 Copy to USB/network folder; image name 1, image name 2, image name 3 ... User name 2020-10-19 3:47:00 Image Send to mobile; image name 1, image name 2, image name 3 ... User name 2020-10-19 3:47:00 System Create/Delete confluency module; module name User name 2020-10-19 3:47:00 Mateo FL User Manual Technical Data...
- Page 92 System Delete backup file; file name User name 2020-10-19 3:47:00 System Update software; Vxxx to Vxxx User name 2020-10-19 3:47:00 System Export audit trails Admin 2020-10-19 3:47:00 System Delete audit trails Admin 2020-10-19 3:47:00 Technical Data Mateo FL User Manual...
- Page 93 Mateo FL User Manual Technical Data...
- Page 94 CONNECT WITH US! Leica Microsystems CMS GmbH | Ernst-Leitz-Strasse 17-37 | 35578 Wetzlar (Germany) | Tel. +49 (0) 6441 29-0 | F +49 (0) 6441 29-2599 www.leica-microsystems.com...
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