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Cryogenic Freezer
User
Manual
Version: CVFM-202401
CryoMatrix Series
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Please read this manual carefully before use.
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The appearance of the product is subject to the actual product.
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In case of product technology or software upgrade, without prior notice.
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Antech Scientific reserves the right of explanation.
© all rights reserved
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Table of Contents
Related Manuals for Antech CryoMatrix Series
Summary of Contents for Antech CryoMatrix Series
- Page 1 Please read this manual carefully before use. ○ The appearance of the product is subject to the actual product. ○ In case of product technology or software upgrade, without prior notice. ○ Antech Scientific reserves the right of explanation. © all rights reserved...
- Page 2 Qingdao Antech Scientific Co., Ltd. No. 182 Haier Road, Qingdao, 266100, China +86 532 8789 0321 | info@antechscientific.com | www.antechscientific.com This manual reviews the operation and maintenance of Antech CryoMatrix Series Cryogenic Freezer. This manual covers below models: CryoMatrix 15K CryoMatrix 19K...
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Page 3: Table Of Contents
CryoMatrix Series Cryogenic Freezer Manual Contents Introduction ..........................4 Safety ............................5 Liquid Nitrogen (LN2) ....................... 5 Frostbite Hazard .......................5 Asphyxiation Hazard ......................6 Over pressurization Hazard ..................... 6 Pinch and Nip Hazard ...................... 6 Product Information ........................7 Freezer Specifications ..................... 7 Construction ........................ - Page 4 CryoMatrix Series Cryogenic Freezer Manual 5. LN2 Usage ......................... 21 6. Temperature Measurement ..................21 7. Data Storage ......................22 8. Alarm .......................... 23 9. Log Data ........................24 1.0 Battery Backup ......................25 Preventative Maintenance .......................25 Troubleshooting ........................26 Repair Safety Instruction ......................27 Appendix ...........................
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Page 5: Introduction
This manual reviews the operation and maintenance of Antech Cryo Freezers. Read before using this equipment. Antech Cryo Freezers are designed for safe and efficient storage of biological samples in dry liquid nitrogen (LN2) vapor at -190°C (-310°F). This is a stainless steel, vacuum- insulated, non pressurized Dewar. -
Page 6: Safety
CryoMatrix Series Cryogenic Freezer Manual Safety This section reviews the safety guidelines for Antech Cryo Freezers. Read before using this equipment. This product is intended for use by trained personnel only. All service and maintenance should be performed by Antech or authorized Antech representatives. -
Page 7: Asphyxiation Hazard
CryoMatrix Series Cryogenic Freezer Manual Asphyxiation Hazard Although nitrogen is nontoxic, it can act as an asphyxiant by displacing oxygen in the air to levels below that required for breathing. Inhalation of excessive nitrogen can cause dizziness, nausea, vomiting, loss of consciousness, and death. Personnel, including rescue workers, should not enter areas where oxygen concentration is below 19.5%, unless... -
Page 8: Product Information
CryoMatrix Series Cryogenic Freezer Manual Product Information Freezer Specifications Model Vials Capacity, Square Rack 1.2 & 2 ml vials (internally 15,600 19,500 28,000 39,200 44,200 55,500 94,500 threaded) Qty of rack (100- cell box) Qty of rack (25- cell box) -
Page 9: Construction
CryoMatrix Series Cryogenic Freezer Manual Wi-Fi connection Optional Optional Optional Optional Optional Optional Optional & cloud access Construction CryoMatrix Series Freezer Construction Diagram 1. Touch screen controller Advanced PLC + Touch screen control system 2. Lid The ergonomic lid design ensures safety and easy use of freezer It consists of three parts: the upper and lower heads and the middle straight cylinder. -
Page 10: Control System
CryoMatrix Series Cryogenic Freezer Manual Control System The control system is designed to automatically maintain the freezer LN2 level while monitoring and logging storage conditions. It consists of a custom controller and touchscreen monitor. The RS232 port and USB port are located at the back of the controller. -
Page 11: Plumbing System
Relative Humidity Less than 50%, non-condensing Seismic restraints may be required in earthquake prone areas. Seismic Restraint Please follow local standards and regulations. Contact Antech for more information. Regulatory Antech Cryo Freezers meet the following safety requirements conforming to Low Voltage... -
Page 12: Environmental
CryoMatrix Series Cryogenic Freezer Manual Directive (2014/35/EU) Environmental 1. Thermal Load Freezer thermal load is negligible with no mechanical refrigeration. Vaporization of LN2 within the insulated freezer provides cryogenic temperatures. 2. Noise Emission Freezer noise emission is negligible with no mechanical refrigeration. Flow noise is generated by the pressurized LN2 supply when relief valves release excess pressure or when the freezer is purging or filling. -
Page 13: Installation
Installation Setup and Initial Fill Antech Cryo Freezers arrive ready for a plug and play setup. The control system is preinstalled with temperature and level sensors factory calibrated. A convenient initial fill routine avoids nuisance alarms as the freezer cools down. The initial fill takes longer and uses more LN2 than a normal fill. -
Page 14: Touch Screen Interface
CryoMatrix Series Cryogenic Freezer Manual Touch Screen Interface 1. Home page The Home page displays the current freezer status and storage conditions. Home Page Including: ○ Date & Time ○ Alarm information: if there is any alarm, it will show on top of screen in red ○... -
Page 15: Parameter Setting
Filling Set, Temperature Alarm set and Other Set. The freezer has been set properly in factory. We recommend to get professional suggestions from Antech or its authorized service agency before changing these settings, so as not to cause any unexpected problems. -
Page 16: Advanced Settings
CryoMatrix Series Cryogenic Freezer Manual Scheduled fill: Fill the LN2 on specified timeTemp Alarm: ○ HT Top: high temperature alarm for top sensor ○ LT Top: low temperature alarm for top sensor ○ HT Bot: high temperature alarm for bottom sensor ○... -
Page 17: System
CryoMatrix Series Cryogenic Freezer Manual 4. System On the System Settings tab you can set time & date, choosing language (English / Chinese), set backlight time and data storage interval. Setting Page: System settings Tab 5. User Settings On the System Settings tab, you can set the authorization to operate this freezer. You can add, delete and change the ID and password. - Page 18 CryoMatrix Series Cryogenic Freezer Manual There are 3 operation authorization level: Operator, Admin and Super Admin Super Admin level can set different ID, password and operation level to different people Once the Super Admin make the Enable On, the display below: User requires to login and manual log out by click the icon in the red rings above.
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Page 19: Default Settings
CryoMatrix Series Cryogenic Freezer Manual 6. Default Settings Each freezer comes preloaded with default settings tailored to vapor storage. Temperature sensors are placed at the top and bottom of the storage space to capture the full range of sample temperatures. Verify and adjust these settings as needed. -
Page 20: Operation
CryoMatrix Series Cryogenic Freezer Manual Operation 1. LN2 Vapor Storage Cryopreservation is the storage of material below -135°C (-211°F) in order to provide longevity to bio-specimens. Around -135°C is referred to as the glass transition point (Tg) of polyol’s water solution. It is generally accepted that below Tg, biological activity essentially ceases. -
Page 21: Ln2 Level Measurement
CryoMatrix Series Cryogenic Freezer Manual 3. LN2 Level Measurement A differential pressure system is used to measure the LN2 level inside the freezer. This system is based on the principle that the pressure generated by a fluid column is proportional to the depth of that fluid column. The difference between the pressure measured at the inside bottom of the freezer and atmospheric pressure will determine the pressure generated by the LN2. -
Page 22: Ln2 Usage
CryoMatrix Series Cryogenic Freezer Manual Following the initial fill, the LN2 level will slowly decrease as LN2 boils off until the Low Fill setting is reached. Prior to starting the fill, a prefill purge is initiated to exhaust warm gas from the freezer plumbing and supply lines. -
Page 23: Data Storage
CryoMatrix Series Cryogenic Freezer Manual 7. Data Storage The freezer storage conditions are logged and displayed in the controller data file. This time stamped event log provides an unalterable electronic history of freezer performance for traceability, record keeping, and troubleshooting. Data can be displayed as graph or pdf. -
Page 24: Alarm
CryoMatrix Series Cryogenic Freezer Manual 8. Alarm Click on Alarm data, it shows historical alarm below: Find: check the historical alarm data on specified date Export: export Data to USB disk. Only can be used when USB disk correctly connected... -
Page 25: Log Data
CryoMatrix Series Cryogenic Freezer Manual 9. Log Data Click LOG DATA, it shows the historical operation log below Setting Page: LOG DATA Tab Find: check the historical alarm data on specified date Export: export Data to USB disk. Only can be used when USB disk correctly connected... -
Page 26: Battery Backup
Main power switch Battery switch Preventative Maintenance This is the recommended preventative maintenance schedule for Antech Cryo Freezers. Preventative maintenance is performed while the equipment is operating properly to reduce the likelihood of potential failure. Content... -
Page 27: Troubleshooting
CryoMatrix Series Cryogenic Freezer Manual Verify Temperature Sensor Calibration Solenoid Valve Replacement Relief Valve Replacement Temperature Sensor Replacement Battery Replacement Lid Gasket Replacement Thaw, Decontaminate, and Dry Freezer Troubleshooting Symptom Causes ● Insufficient LN2 supply volume and/or pressure Freezer Not Filling Slow Fill LN2 supply incorrectly connected ●... -
Page 28: Repair Safety Instruction
Failure to observe these precautions can result in severe injury. All service and maintenance the Safety section of should be performed by Antech or trained Antech representatives. Refer to this manual for more information. Appendix... -
Page 29: Altitude - Ln2 Temperature Table
CryoMatrix Series Cryogenic Freezer Manual BM-011 LN2 Transfer Hose CGA-295 | 2 meters | Vacuum Jacketed BM-012 Backup Battery BM-013A Lid insulation Foam For CryoMatrix 15K/19K BM-013B Lid insulation Foam For CryoMatrix 28K/39K BM-013E Lid insulation Foam For CryoMatrix 44K... -
Page 30: Ln2 Level - Pressure Units Table
CryoMatrix Series Cryogenic Freezer Manual LN2 Level - Pressure Units Table in LN2 in H20 in LN2 in H20 0.02 0.16 0.03 0.18 0.04 0.19 0.06 0.21 0.07 0.22 0.09 0.23 0.10 0.24 0.12 0.26 0.13 0.28 0.15 10.0 0.29... -
Page 31: Warranty
During the first year of the warranty period, component parts proven to be non-conforming in materials or workmanship will be provided by Antech free of charge (international express fee not included). Installation and calibration is not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to any work being performed. - Page 32 Qingdao Antech Scientific Co., Ltd. Tel: +86 532 87890321 Email: info@antechscientific.com Web: www.antechscientific.com...
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