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InnovaPrep FLUIDPREP CP Select User Manual

Concentrating pipette and concentrating pipette system
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Concentrating Pipette (CP Select™)
and Concentrating Pipette System
User Manual

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Summary of Contents for InnovaPrep FLUIDPREP CP Select

  • Page 1 Concentrating Pipette (CP Select™) and Concentrating Pipette System User Manual...

  • Page 2: Table Of Contents

    PTIONS HIS CAN BE TURNED ON IN YSTEM ETTINGS ............................ 27 EFAULT ONCENTRATION ROTOCOLS ................................27 ELECTING A ROTOCOL ..............................28 REATING A USTOM ROTOCOL ..............................28 DITING A USTOM ROTOCOL ................................. 29 ELETING A ROTOCOL Rev B InnovaPrep LLC...
  • Page 3 ................................29 ASSWORD ROTECTION ..........................29 ONCENTRATING IPETTE PERATION HECK TIPS AND SUGGESTIONS ..............................30 CONCENTRATING PIPETTE SUPPLIES ............................ 31 CHEMICAL COMPATIBILITY GUIDE ............................32 CUSTOM PROTOCOL RECORDS ............................34 Rev B InnovaPrep LLC...

  • Page 4: Product Overview

    RODUCT VERVIEW The InnovaPrep Concentrating Pipette System is part of the FluidPrep product line, which revolutionizes how particles are concentrated from liquids for a wide variety of applications, including microbial analysis. 1.1 P RODUCT ESCRIPTION The InnovaPrep Concentrating Pipette Select (CP Select™), shown in Figure 2.1, quickly and efficiently concentrates particles of interest from large liquid sample volumes into liquid volumes as small as 200 microliters (smaller volumes are attainable in some applications).

  • Page 5: Device Specifications - See Cp Select Specifications

    Tubing Clip (HC08017) – Keeps permeate line secure to waste container. • Permeate Line (HC08006) – Directs filtered fluid to waste container. See Section 5.2. • Silicone Lubricant (HC08535) – Used for required, periodic lubrication of O-rings in the Tip Interface Port. See • Section 5.5. Rev B InnovaPrep LLC...

  • Page 6: Safety Precautions

    BIOHAZARD SYMBOLS Use of the InnovaPrep Concentrating Pipette in the laboratory is subject to general safety provisions for use of microbiological instrumentation. Please refer to your laboratory’s current SOPs and the guidance located in the Biosafety in Microbiological and Biomedical Laboratories, 6th Edition (BMBL}: https://www.cdc.gov/labs/pdf/CDC-BiosafetyMicrobiologicalBiomedicalLaboratories-2020-P.pdf...
  • Page 7 SYMBOLES DE MISE EN GARDE OU DE RISQUE BIOLOGIQUE L’utilisation de la Pipette de concentration InnovaPrep au laboratoire est soumise aux dispositions de sécurité générales pour l’utilisation d’instruments microbiologiques. Consultez les procédures normalisées d’exploitation (SOP) en vigueur dans votre laboratoire et les instructions (en anglais) du manuel Biosafety in Microbiological and Biomedical Laboratories, 6e édition (BMBL} :...

  • Page 8: Concentrating Pipette Method Of Operation

    ETHOD OF PERATION The InnovaPrep concentration process uses dead-end filtration to capture particles onto the surface of a porous membrane filter. A novel Wet Foam Elution™ process is then employed to wash the particles off of the membrane surface into very small liquid volumes.

  • Page 9: Foam Generation

    Wet Foam Elution allows the user to select the fluid that the particles will be suspended in after concentration, which reduces inhibition and maximizes the chances of detection. 2.4 CP F (CPT ILTER For selecting the proper CPT please refer to the Consumable Selection Guide Rev B InnovaPrep LLC...

  • Page 10: Components Of The Concentrating Pipette

    OMPONENTS OF THE ONCENTRATING IPETTE The following Section describes the components of the InnovaPrep Concentrating Pipette Select. Fluidics Head Tip Interface Ports Single-use Pipette Tip Canister Latch Elution Fluid (Foam) Canister Maintenance Tip Canister Interface Port Sample Platform Storage Fluid w/...
  • Page 11 Enter ( ⏎ ) down for approximately 1 second. Sample Platform – The sample container is placed here during sample processing. Tension Knob USB Port Power Switch 12V DC Power Permeate Line Port Rev B InnovaPrep LLC...
  • Page 12 Power Switch – The up position ( | ) turns the instrument on and the down position (  ) turns the instrument off. Permeate Line Port – This quick connect fitting is where the filtered fluid (Permeate) exits the instrument. This port is valved to prevent fluid from being pumped out without the permeate tubing attached. Rev B InnovaPrep LLC...

  • Page 13: Installation Of The Instrument Components

    The Bung can now clip onto the bracket as shown, with the Fluids Adapter tubing clipped into the bracket behind the bottle. The Maintenance Tip also stows on the bracket in the 1 position. See Section 7 for detailed instructions for use. Rev B InnovaPrep LLC...

  • Page 14: Installing And Removing A N Elution Fluid Canister

    Utilize the protective orange cap (HC03173) included with the unit for long term storage. Clean the Canister Interface Port using an Alcohol Prep Pad prior to installing a new elution fluid canister. Rev B InnovaPrep LLC...

  • Page 15: Installing And Removing Concentrating Pipette Tips

    To remove the CPT, grasp it with your hand then use your thumb to press up on the underside of the Fluidics Head. The "thumbs up" technique eliminates the need to hold the Fluidics Head with your free hand while you pull down on the CPT. Rev B InnovaPrep LLC...

  • Page 16: Navigating The Menu

    3.1 CPT Selection Guide for a full Table description. • Selecting Start Run on the Run Protocols screen will immediately start the sampling process using the sample protocol notated above. Return will lead back to the Main Menu. • Rev B InnovaPrep LLC...

  • Page 17: The Maintenance Menu

    11 advanced settings during Protocol Creation. Once set, the options are accessed in the Protocol Setup menu under Create Protocol. Default = Std Fluid Gauge turns the Elution Fluid Volume gauge on and off. Default = on • • Return will return you to the Options menu. Rev B InnovaPrep LLC...

  • Page 18: Maintenance Procedures

    Step 1 – Ensure the required instrument components have been installed: Power Supply (Section 5.1), Permeate Line (Section 5.2), Storage Fluid and Adapter (Section 5.3). Step 2 – Power instrument on. Advance to the Main Menu by pressing Enter (⏎) on the control panel. Step 3 – Select Maintenance>Start Up Rev B InnovaPrep LLC...

  • Page 19: The Shut Down Procedure

    For instruments with firmware versions V6.0.0.0 or later: If the CP Select is turned off prior to performing a shut down procedure, a notice will appear on the menu stating, CP not properly shut down. Please run a shut down. If this message appears, select maintenance>shut down and follow the menu prompts. Rev B InnovaPrep LLC...

  • Page 20: Priming The Elution Fluid

    Step 11 – Continue past the menu prompt: Insert Storage Fluid Line into Can Port Select Continue. The canister port must remain open to allow fluid to be flushed out of the permeate port. Rev B InnovaPrep LLC...
  • Page 21 Step 13 – Place the orange Canister Interface Port cap that came with the instrument over the Canister Interface Port. Step 14 – Remove and drain the Permeate Line. Step 15 – Use the original Foam Packaging and Box for all transport and shipments. Rev B InnovaPrep LLC...

  • Page 22: Recommended General Maintenance For

    Inspect this tube set regularly Adapter for proper operation during startup and shutdown routines. Annual 12 Months Contact InnovaPrep or your distributor for The pressure transducer should be calibrated Maintenance and return material authorization. every 12 months. Calibration *Failure to follow recommended maintenance procedures could cause your instrument to perform poorly and could void warranty.

  • Page 23: Performing A Sample Concentration Run

    Continue with the Wash step as you would with a normal concentration run and follow the prompts to FIGURE 7.1 ALTERNATE WASH FUNCTION perform an Elute step or a second Wash step. Rev B InnovaPrep LLC...

  • Page 24: Controlled Collection Of Permeate

    In cleanroom settings, the Concentrating Pipette user may desire to collect the permeate fluid in an enclosed container fitted with a vent filter. This eliminates the remote possibility of airborne contamination. InnovaPrep has a Permeate Bottle with a Vent Filter available for this use (item HC08530). It comes with a 2-port closure consisting of a 0.2 µm vent filter and an 18-inch length of tubing.

  • Page 25: Concentration Run Protocols

    Pulse Count is the number of cycles that the elution valve will open and close. Multiple pulses are usually used when larger (>200 µL) final volumes are desired. For example; in some situations, recovery may be more efficient Rev B InnovaPrep LLC...
  • Page 26 Changes to the Valve Open time and Pulse count should be considered first. seconds, Min= 0.1 seconds, Max = 10.0 seconds Reset all defaults resets all settings on the unit to the factory defaults, and custom protocols will be lost. Return will return to the Select Protocol menu. Rev B InnovaPrep LLC...

  • Page 27: Efault Oncentration Un Rotocols

    Step 2 – Select Select Protocol. Using the Up arrow ( ▲ ) and Down arrow ( ▼ ) you may now go to each available menu with the Protocol name at the top and standard or advanced settings for the protocol below. Standard Rev B InnovaPrep LLC...

  • Page 28: Creating A Custom Protocol

    System Settings. Advanced settings will be shown if Protocol: Adv has been previously selected in the System Settings. This process is described in Section 5.4 The Instrument Settings Menu. Make the needed edits. Step – 2 Select Save & Return. The user is then returned to the Protocol Setup menu. Rev B InnovaPrep LLC...

  • Page 29: Deleting A Protocol

    Insert an Elution Fluid Canister into the Canister Interface Port and check for leaks. b. From the Main Menu select Maintenance>Prime and follow the menu prompts. Check for production of foam. c. Remove Elution Fluid Canister. d. Ensure there is no leakage from the Canister Interface Port. Rev B InnovaPrep LLC...

  • Page 30: Tips And Suggestions

    Clean CP Select’s surface areas with damp cloth-10% bleach solution or 3% hydrogen peroxide. • Use a 70% isopropyl alcohol prep pad to wipe down the CPT interface ports on the underside of the Fluidics Head between CPTs and the Canister Interface Port between Fluid Canisters. Rev B InnovaPrep LLC...

  • Page 31: Concentrating Pipette Supplies

    10 C ONCENTRATING IPETTE UPPLIES CONSUMABLE SELECTION GUIDE ALL ITEMS CAN BE PURCHASED DIRECTLY FROM THE INNOVAPREP WEBSITE, HTTP://INNOVAPREP.COM/STORE OR BY CONTACTING CUSTOMER SERVICE CUSTOMERSERVICE@INNOVAPREP.COM 816 -619-3375. Rev B...

  • Page 32: Chemical Compatibility Guide

    Carbon tetrachloride Chloroform Chromic acid Cresol Cyclohexanone Diacetone alcohol Dimethyl formamide Dimethyl sulfoxide Ethers Ethyl acetate Ethyl Alcohol Fuels 50% Ethyl alcohol 95% Ethyl alcohol Ethylene dichloride Ethylene glycol 10% Formaldehyde 30% Formaldehyde 50% Formaldehyde Glycerin Hexane Rev B InnovaPrep LLC...
  • Page 33 Perchloroethylene 0.5% Phenol 10% Phenol 0.1 N Sodium Hydroxide Conc. Sodium Hydroxide Sodium hypochlorite (10% bleach solution) 5% Sulfuric acid 25% Sulfuric acid Conc. Sulfuric Acid Toluene Trichloroethane Trichloroethylene Triton (surfactant solution) Tween (surfactant solution) Water Rev B InnovaPrep LLC...

  • Page 34: Custom Protocol Records

    Sec. Sec. Protocol Name: _________________________________________________________________________________ Date: ___________________ Created by: ___________________________________________________________ Created for: ____________________________________________________________________________________ Valve Pulse Foam Valve Flow Flow Flow Pump Open Factor Closed Start Delay Pump Protocol Name Sec. Sec. Start Sec. Delay Sec. Sec. Rev B InnovaPrep LLC...
  • Page 35 © 2018-2023 InnovaPrep LLC. All rights reserved. InnovaPrep, FluidPrep, CP Select, Concentrating Pipette, Wet Foam Elution are trademarks or registered trademarks of InnovaPrep LLC. All other brands and names contained herein are the property of their respective owners. Patented https://www.innovaprep.com/patents...

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