InnovaPrep CP Select User Manual
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InnovaPrep CP Select User Manual

Concentrating pipette select
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Copyright 2018, InnovaPrep LLC
Patented
https://www.innovaprep.com/patents
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Summary of Contents for InnovaPrep CP Select

  • Page 1 Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 2: Table Of Contents

    ONCENTRATION ROTOCOLS ................................27 ELECTING A ROTOCOL ..............................27 REATING A USTOM ROTOCOL ..............................28 DITING A USTOM ROTOCOL ................................28 ELETING A ROTOCOL ................................28 ASSWORD ROTECTION ..........................29 ONCENTRATING IPETTE PERATION HECK Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...
  • Page 3 TIPS AND SUGGESTIONS ............................... 30 SAFETY PRECAUTIONS ..............................30 CONCENTRATING PIPETTE SUPPLIES ..........................32 CHEMICAL COMPATIBILITY GUIDE ............................ 34 CUSTOM PROTOCOL RECORDS ............................36 REVISION HISTORY ................................37 Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 4: Product Overview

    200 microliters (smaller volumes are attainable in some applications). The CP Select uses a novel elution method to recover particles that have been captured onto a disposable porous membrane filter with high recovery efficiencies.

  • Page 5: Ccessories Included With The Cp Select

    1.4 A CP S CCESSORIES INCLUDED WITH THE ELECT An image and list, including part numbers, of the accessories that are provided with the CP Select is provided below. Power Cord 11 µm Nylon Syringe Alcohol Prep Pads Filter 25mm...
  • Page 6 Filterless Tip (HC08524 - M) – Used for alternate cleaning and maintenance procedures. Also, for • troubleshooting and diagnostic procedures when consulting with InnovaPrep Technical Support Engineers. Keep on hand. 11 µm Nylon Syringe Filter 25mm (HC08533) – Used for alternate cleaning and maintenance procedures.

  • Page 7: Concentrating Pipette Method Of Operation

    4. When the user performs an elution, InnovaPrep’s patented Wet Foam Elution™ process is utilized. The Permeate Port valve closes and the elution valve opens, allowing the foam to enter the Elution Port. The foam travels tangentially down the surface of the membrane as it washes the particles from the surface.

  • Page 8: Wet Foam Elution

    2.4 CP F ILTER For selecting the proper CPT please refer to the filter selection matrix presented in Table 2.1. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...
  • Page 9 30 minutes. The recommended upper-end run volume as shown in the concentrating pipette tip selection guide and time of 30 minutes is a general recommendation and may be dependent on sample matrix and target organism. See Section 10 for full part number list. Copyright 2018, InnovaPrep LLC Patented...

  • Page 10: Components Of The Concentrating Pipette

    OMPONENTS OF THE ONCENTRATING IPETTE The following Section describes the components of the InnovaPrep Concentrating Pipette Select. Fluidics Head Tip Interface Ports Single-use Pipette Tip Canister Latch Elution Fluid (Foam) Canister Maintenance Tip Canister Interface Port Sample Platform Storage Fluid w/...
  • Page 11 Enter ( ⏎ ) down for approximately 1 second. Sample Platform – The sample container is placed here during sample processing. Tension Knob USB Port Power Switch 12V DC Power Jack Permeate Line Port FIGURE 3.2 INSTRUMENT COMPONENTS LEFT SIDE – Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...
  • Page 12 Power Switch – The up position ( | ) turns the instrument on and the down position ( ¡ ) turns the instrument off. Permeate Line Port – This quick connect fitting is where the filtered fluid (Permeate) exits the instrument. This port is valved to prevent fluid from being pumped out without the permeate tubing attached. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 13: Installation Of The Instrument Components

    100 to 240 VAC, 50-60 Hz, single phase. The connection is self-regulating. WARNING: DO NOT USE ANY POWER SUPPLY OTHER THAN THE ONE SUPPLIED WITH THE CP SELECT INSTRUMENT-DOING SO MAY CAUSE DAMAGE TO THE INSTRUMENT AND VOID THE WARRANTY. (NOTE: THE POWER SUPPLY FOR THE PREVIOUSLY RELEASED CP - 150 CONCENTRATING PIPETTE INSTRUMENT IS NOT COMPATIBLE WITH THE CP SELECT) 4.2 I...

  • Page 14: Installing And Removing A N Elution Fluid Canister

    FIGURE 4.4. STORAGE FLUID AND FIGURE 4.3 STORAGE FLUID AND STORAGE FLUID STORAGE FLUID ADAPTER ADAPTER STOWED ON CP SELECT BRACKET 4.4 I NSTALLING AND EMOVING LUTION LUID ANISTER To install an elution fluid canister into the instrument, first hold the canister with the valve pointing down.

  • Page 15: Installing And Removing Concentrating Pipette Tips

    To remove the CPT, grasp it with your hand then use your thumb to press up on the underside of the Fluidics Head. The "thumbs up" technique eliminates the need to hold the Fluidics Head with your free hand while you pull down on the CPT. Copyright 2018, InnovaPrep LLC Patented...

  • Page 16: Navigating The Menu

    2.1 CPT Selection Guide for a full Table description. • Selecting Start Run on the Run Protocols screen will immediately start the sampling process using the sample protocol notated above. • Return will lead back to the Main Menu. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 17: The Maintenance Menu

    11 advanced settings during Protocol Creation. Once set, the options are accessed in the Protocol Setup menu under Create Protocol. Default = Std • Fluid Gauge turns the Elution Fluid Volume gauge on and off. Default = on • Return will return you to the Options menu. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 18: Maintenance Procedures

    Section 4: Install Power Supply (Section 4.1) and Permeate Line (Section 4.2), and stow the Storage Fluid and Adapter in the bracket on the right side of the instrument (Section 4.3). Step 3 – Power instrument on. FIGURE 6.1 MAINTENANCE TIP Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 19: The Start U P Procedure

    The Shut Down procedure performs two important functions, instrument decontamination and the prevention of salt crystals forming in the fluidics due to evaporation of the elution fluid. Step 1 – From the Main Menu select Maintenance>Shut Down. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 20: Priming The Elution Fluid

    Step 5 – At the menu prompt: Power Off Unit or Restart. Leave the Maintenance Tip and Storage Fluid Adapter in place and Power Off the unit. Step 6 – Allow the instrument to sit for 30 minutes to ensure complete decontamination of the fluidics. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...
  • Page 21 Step 13 – Place the orange Canister Interface Port cap that came with the instrument over the Canister Interface Port. Step 14 – Remove and drain the Permeate Line. Step 15 – Use the original Foam Packaging and Box for all transport and shipments. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 22: Recommended General Maintenance For

    Eliminates "salt crystals" in CP Select fluid Routine Daily lines. Perform Shut Down After day's use Main Menu > Maintenance > Shut Down Eliminates " salt crystals " in CP Select fluid Routine Daily lines. Lubricate O - rings HC08535 Every 2 weeks Available at www.Innovaprep.com or your...

  • Page 23: Performing A Sample Concentration Run

    In cleanroom settings, the Concentrating Pipette user may desire to collect the permeate fluid in an enclosed container fitted with a vent filter. This eliminates the remote possibility of airborne contamination. InnovaPrep has a Permeate Bottle with a Vent Filter available for this use (item HC08530). It comes with a 2-port closure consisting of a 0.2 µm vent...
  • Page 24 Permeate Port is pushed down on the instrument. Then, insert the quick connect fitting on the permeate tubing into the Permeate Port. When the fitting is fully seated the metal button will pop up, thus locking it in place. Replacement Permeate Vent Filters are also available from InnovaPrep in packs of five filters (item HC08532-5). Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 25: Concentration Run Protocols

    (>200 µL) final volumes are desired. For example; in some situations, recovery may be more efficient if two 100-ms pulses are performed rather than one 200-ms pulse while maintaining the same final elution volume. Min = 1ms, Max = 25ms Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 26: Default Concentration Run Protocols

    CC08020), and ULTRA is for use with the hollow fiber ultrafilters (item CC08003). Table 8.1 shows the standard set points (shown in blue) and advanced menu set points (shown in orange) for each protocol: Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 27: Selecting A Protocol

    Step 1 – You will then be taken to a menu with the created Protocol name at the top and standard or advanced settings for the protocol below. Standard settings will be shown if Protocol: Std has been previously selected in Copyright 2018, InnovaPrep LLC Patented...

  • Page 28: Editing A Custom Protocol

    In order to allow users to control the creation, modification, or deletion of custom protocols, a password protection is provided with the CP Select. Passwords can be setup by selecting Systems Settings>Set Password from the Main Menu. If a password has not been previously set up then the user will see the Set Password screen and can create a password.

  • Page 29: Concentrating Pipette Operation Check List

    8. Look inside the head of the unit and check for any visible fluid leaks or moisture inside the head. Failure in any of the above tests indicates a return of the Concentrating Pipette for repair may be necessary. Copyright 2018, InnovaPrep LLC Patented...

  • Page 30: Tips And Suggestions

    IPS AND UGGESTIONS This Section contains some dos and don'ts, tips, tricks and important notes to remember while using the CP Select. • The instrument can concentrate and elute with the Fluidics Head in any position. • Never run alcohol > 15% through the instrument as it may damage internal components.
  • Page 31 Select fits conveniently within a biosafety cabinet; use of such containment is recommended. For work with BSL3 or BSL4 samples, refer to and follow your laboratories SOPs for use and care of laboratory equipment. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 32: Concentrating Pipette Supplies

    ONCENTRATING IPETTE UPPLIES THE FOLLOWING TABLE CONTAINS A LIST OF THE SUPPLIES THAT CAN BE PURCHASED FROM INNOVAPREP FOR USE WITH THE CONCENTRATING PIPETTE. ALL OF THESE ITEMS CAN BE PURCHASED DIRECTLY FROM THE INNOVAPREP WEBSITE, HTTP://INNOVAPREP.COM/STORE OR BY CONTACTING CUSTOMER SERVICE CUSTOMERSERVICE@INNOVAPREP.COM...
  • Page 33 Part Number Item External Power Supply – CP Select HC08584 12v Power supply for field use. HC08535-20 Silicone Lubricant - 1cc Tube, 20pk Elution Port Cap HC03173 Protects the fluid canister interface port while the Concentrating Pipette is not in use.

  • Page 34: Chemical Compatibility Guide

    Cyclohexanone Diacetone alcohol Dimethyl formamide Dimethyl sulfoxide Ethers Ethyl acetate Ethyl Alcohol Fuels 50% Ethyl alcohol 95% Ethyl alcohol Ethylene dichloride Ethylene glycol 10% Formaldehyde 30% Formaldehyde 50% Formaldehyde Glycerin Hexane 5% Hydrochloric acid Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...
  • Page 35 0.5% Phenol 10% Phenol 0.1 N Sodium Hydroxide Conc. Sodium Hydroxide Sodium hypochlorite (10% bleach solution) 5% Sulfuric acid 25% Sulfuric acid Conc. Sulfuric Acid Toluene Trichloroethane Trichloroethylene Triton (surfactant solution) Tween (surfactant solution) Water Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 36: Custom Protocol Records

    Protocol Name: _________________________________________________________________________________ Date: ___________________ Created by: ___________________________________________________________ Created for: ____________________________________________________________________________________ Valve Pulse Foam Valve Flow Flow Flow Pump Open Factor Closed Start Delay Pump Protocol Name Sec. Sec. Start Sec. Delay Sec. Sec. Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

  • Page 37: Revision History

    September 2017 A. Packingham Rev 2 October 2017 A. Packingham Rev 3 January 2018 A. Packingham Rev 4 March 2018 A. Packingham Rev 5 April 2018 D. Alburty Rev 6 February 2020 J. Birkenholz Copyright 2018, InnovaPrep LLC Patented https://www.innovaprep.com/patents...

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