Page 1 228-30883G May 2005 SOLVENT DELIVERY MODULE FOR SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH LC-20AB INSTRUCTION MANUAL Read the instruction manual thoroughly before you use the product. Keep this instruction manual for future reference.
Page 2 This page is intentionally left blank.
Page 3 • To ensure safe operation, read the Safety Instructions before using the instrument. COPYRIGHT • Copyright (C) 2004-2005 Shimadzu Corporation. All rights reserved. This publication may not be reproduced in whole or in part without written permission from Shimadzu Corporation. Since Shimadzu products are frequently upgraded and improved, information in this publication is subject to change without notice.
Page 4: Warranty And After-Sales Service
Introduction Warranty and After-Sales Service Warranty Validity Please consult your Shimadzu representative for information about the extent of the warranty. Term The manufacturer will provide free replacement parts for, or repair free of charge, any instrument that fails during the warranty period, if the cause can be attributed to a defect in manufacturing.
Page 5 "7 Hardware Validation" P. 7-1 Hardware Validation This is a contract under which a qualified Shimadzu-approved engineer performs periodic component and system validation, and provides reports of Contract the results. Details of the contract can be obtained from your Shimadzu representative. LC-20AB...
Page 6: Safety Instructions
Application Precautions WARNING This instrument is a solvent delivery module for use with a high performance liquid chromatography system. Use this instrument ONLY for the intended purpose. Using this instrument for any other purpose could cause accidents. LC-20AB...
Page 7: Installation Site Precautions
Otherwise, the instrument could tip over or fall off the table. At least 600mm • Avoid installation sites that are exposed to corrosive gases or excessive dust. These adverse conditions may be detrimental to maintaining the instrument performance and may shorten its service life. LC-20AB...
Page 8: Installation Precautions
The cord could be damaged, resulting in fire, electrical shock or malfunction. If the cord becomes damaged, contact your Shimadzu representative immediately. • Do not modify the cord in any way. Do not bend it excessively or pull on it.
Page 9: Operation Precautions
It could break the reservoir bottles and cause injury. • Do not use flammable sprays (hair sprays, insecticide sprays, etc.) near the instrument. They could ignite and cause a fire. LC-20AB...
Page 10: Precautions For Instrument Inspection, Maintenance, Adjustment And Care
• Never remove the main cover. This may cause injury or malfunction of the instrument. The main cover does not need to be removed for routine maintenance, inspection and adjustment. Have your Shimadzu representative perform any repairs requiring removal of the main cover.
Page 11: Static Electricity Precautions
Air bubbles in liquid facilitate Ignition of flammable substances generation of static electricity. Insulated container of spark polyethylene, etc. Dry air Flammable gas present in container. Flammable organic solvent with spark large electrostatic charge. Floor covered with rubber, etc., cannot conduct electricity away. LC-20AB...
Page 12: Preventing Static Electricity Accidents
Connect clip to metal parts. Connect to ground terminal or other grounding point of the Reduce the opening instrument to ground metal with a cap. container. 18 Liter metal can (preferably plated) Liquid's static will be conducted through container to ground. LC-20AB...
Page 13 Use tubing with an inner diameter of at least 2mm for drain lines with high flow rates. CAUTION • Periodically check the tubing connections for leaks. Air bubbles in liquid can multiply the electrostatic charge by a factor of 20, 30 or more. LC-20AB...
Page 14: For Reference
• Use as small a container as possible to minimize damage in the event of fire. • Keep the room at a proper humidity. Ambient humidity exceeding 65% will prevent static. For Reference Anti-static equipment (anti-static clothing, shoes and matting) and charge measurement equipment (potentiometer) are sold by specialty manufacturers. LC-20AB...
Page 15: Precautions For Mobile Phase Selection And Use
• Always degas the mobile phase, as air bubbles may tend to form during solvent mixing or during temperature or pressure changes. Air bubbles may cause pump malfunctions and detector signal noise. • For boiling points, viscosities and other data relating to the mobile phases used, "9.5 Mobile Phase Characteristics" P. 9-44 LC-20AB XIII...
Page 16: Warning Labels
Introduction Warning Labels For safety operation, warning labels are affixed to where special attention is required. Should any of these labels peel off or be damaged, obtain replacements from Shimadzu Corporation. Warning label (Part No. 228-42603) LC-20AB...
Page 17: Table Of Contents
Behind Front Cover ..................2-3 Right Side and Base Panel ................2-5 Back .........................2-6 Names and Functions of Displays and Keypad ..........2-7 2.5.1 Display Panel ....................2-7 2.5.2 Keypad ......................2-8 Chapter 3 Preparation Turning Power ON/OFF ...................3-2 Preparation before Operation ................3-3 LC-20AB Contents...
Page 18 List of Auxiliary Functions ................5-12 Parameter Settings Group ..................5-12 File Operation Group ....................5-12 Control Settings Group ....................5-13 System Settings Group ....................5-13 Monitor Settings Group ....................5-13 5.2.2 Showing the Auxiliary Function Screen ............5-14 5.2.3 Parameter Settings Group ................5-15 LC-20AB Contents...
Page 19 ................5-28 Calibration Support Group 5.3.2 Displaying the VP Functions ................. 5-29 5.3.3 Mobile Phase Monitor Group ................ 5-30 Showing/Setting Mobile Phase Volume [MOBILE PHASE A/B] ......5-30 Setting Alarm Level of Remaining Mobile Phase [ALARM LEVEL] ......5-30 LC-20AB Contents...
Page 20 Deleting Steps ....................5-51 5.4.7 Starting and Stopping a Time Program ............5-51 Starting a Time Program ..................5-51 Stopping a Time Program ..................5-51 5.4.8 Commands Used Only For Time Program ............ 5-52 Setting Stop Command of Time Program [STOP] ...........5-52 LC-20AB Contents...
Page 21 Validation Precautions ..................7-4 7.3.1 Environment ....................7-4 7.3.2 Installation Site ....................7-4 Equipment Required for Validation ..............7-5 Testing Equipment ....................7-5 Standard Reagents for Validation ................7-6 Hardware Testing Supplies ..................7-6 Validation: Pump ....................7-7 7.5.1 Check Terms ....................7-7 LC-20AB Contents...
Page 22 Objective .........................7-23 Items Required for Validation ..................7-23 Checking and Preparing the LC system ..............7-24 Validation Procedure ....................7-25 Parameter Settings for Isocratic System Validation ..........7-26 7.7.2 Validation of Gradient LC System ..............7-27 Objective .........................7-27 Items Required for Validation ..................7-27 LC-20AB Contents...
Page 23 Inspection (Replacement) and Ultrasonic Bath Cleaning of Line Filter ..8-19 Replacing Frit ......................8-20 Inspection (Replacement) and Ultrasonic Bath Cleaning of Suction Filter ..8-21 Replacement of Drain Valve ASSY ..............8-22 Replacement of Fuse ..................8-24 8.10 Wiping of Leak Tray ..................8-25 8.11 Exterior Cleaning ....................8-26 LC-20AB Contents...
Page 24 Mounting the Mixer on the Instrument ..............9-25 Mixer Plumbing ......................9-26 Mixer Cover Installation ...................9-27 9.1.9 Wiring ......................9-28 Connectors ......................9-28 Connecting the Optical Cable ..................9-29 Connection to System Controller ................9-30 Connection to System Controller (in case of the installed CBM-20Alite) ....9-31 LC-20AB Contents...
Page 25 LC-20AB Pump Unit ..................9-37 9.2.2 High-Pressure Gradient System ..............9-38 High-Pressure Gradient System controlled by System Controller (CBM-20A) ..9-38 High-Pressure Gradient System controlled by Pump Unit (LC-20AB) ....9-38 Maintenance Parts ..................9-39 9.3.1 Consumable Parts ..................9-39 9.3.2 Replacement Parts ..................
Page 26 Contents This page is intentionally left blank. LC-20AB Contents...
Page 27 Configuration Contents Overview ......................1-2 Features ......................1-2 Component Parts ..................... 1-3 Optional Parts ....................1-5...
Page 28: Overview
1. Configuration Overview LC-20AB (hereinafter to as the instrument) is a high-pressure gradient solvent delivery module equipped with two dual-plunger tandem-flow pumps. It offers improved accuracy and sensitivity in high-performance liquid chromatograph. In addition to the instrument, a high performance liquid chromatography system requires an autosampler, column oven, detector and system controller at a minimum, which should be purchased separately.
Page 29: Component Parts
These 2-digit figures refer to the last two digits in the product number of the instrument. -31 is for products destined for Japan, while -32 and -38 are for use in other countries . Parts Part No. Q’ty Remark LC-20AB AC Power cord (for UL/CSA) -31, -32 only 071-60825-01 AC Power cord (for VDE) -38 only...
Page 30: Accessory Pack
For line filter 228-32744 Drain OUT, STD 228-42205 Drain OUT, CTO 228-42206 Silicone tubing 228-25162-03 Straight tubing connector 228-28163 L-type tubing connector 035-61561-12 Drain adapter 228-42204 Tubing holder 228-42209 Stop joint D 228-46054-91 Syringe tubing D Volume: 2.5mL 228-46055-91 LC-20AB...
Page 31: Optional Parts
1.4 Optional Parts Optional Parts Optional units available for this instrument are listed below. For information about other optional units not listed below, contact your Shimadzu representative. Option Part No. Features Degasses mobile phase by passing through special tubing made of Degasser (5 flow lines) resin film, thereby reducing the pressure surrounding the tubing.
Page 32 Injector mounting plate Plate for installing manual injectors on the instrument. 228-35659-92 Column holder For use if the column is installed on the instrument. 228-35655-94 CBM-20Alite CBM-20Alite system controller can be installed inside of the instrument. 228-45011-XX LC-20AB...
Page 33 Parts Identification and Function Contents Front Cover ...................... 2-2 Behind Front Cover ..................2-3 Right Side and Base Panel ................2-5 Back ......................... 2-6 Names and Functions of Displays and Keypad ..........2-7...
Page 34: Front Cover
To close the valve, turn back as far as it will go. Front cover To cover pump heads and flow lines. Power switch To switch the power ON/OFF. Press the switch in to turn power ON. Press again to turn power OFF. Press in Press again (power ON) (power OFF) LC-20AB...
Page 35: Behind Front Cover
To enclose a reciprocating plunger which delivers solvent. Pump B inlet Head holder* Inlet check valve* The filtered solvent line is To support the plunger rinse The inlet check valve. connected to this inlet. flow line and the pump head. LC-20AB...
Page 36 The outlet for the rinse flow line. To connect rinse tubing to perform auto rinsing and manual rinsing. ∗ Rinse-solution inlet The inlet for the rinse flow line. To connect rinse tubing to perform auto rinsing and manual rinsing. LC-20AB...
Page 37: Right Side And Base Panel
2.3 Right Side and Base Panel Right Side and Base Panel Mixer mounting holes Used to install mixer, manual injector and column stay. Leakage drain outlet Shipping screws Connect the provided drain tubing. To prevent the shock during transportation. Remove before installation. LC-20AB...
Page 38: Back
(FCV series) or DGU-20A degasser. [PUMP PRESS] connector To output voltage so that pressure can be monitored with an external device. Can be adjusted from 0-5MPa up to 0-50MPa in 10 steps. [DGU PRESS] connector To input the pressure signal from degasser. LC-20AB...
Page 39: Names And Functions Of Displays And Keypad
Illuminates when the instrument is controlled by system controller. (Remote mode indicator) G.E. Illuminates when the instrument is running in gradient mode. (Gradient mode indicator) Green : when power is ON. : when an error is generated. Status Indicator Orange : during sleep mode LC-20AB...
Page 40: Keypad
• To scroll forward through basic functions. Function key • To scroll forward through auxiliary functions. • To scroll during time program editing. Enter key To validate input values for each item setting. Numeric keypad To enter numeric values for each item setting. LC-20AB...
Page 41 Preparation Contents Turning Power ON/OFF ................... 3-2 Preparation before Operation................3-3 Checking Pressure Value................. 3-5...
Page 42: Turning Power On/Off
™™™™™™™™™™™™™™™™ The memory is automatically tested, and after the memory check passes, the version number of the Prominence control program is displayed momentarily. LC-20AB V*.** The screen shown on the right appears and ∗ ∗∗ displays the ROM version [V ] in this example.
Page 43: Preparation Before Operation
Fig. 3.3 Turn the drain valve knob 180° counterclockwise to open the drain valve. Press twice and set the concentration of Open B to 50%. mobile phase Press The pumps run, and the pump indicator illuminates. Drain valve Fig. 3.4 LC-20AB...
Page 44 Press Pump indicator Concentration of mobile phase B The pumps run, and the pump indicator illuminates. After about 15 minutes, press again. The pump stops, and the pump indicator goes out. Preparation for operation is now completed. LC-20AB...
Page 45: Checking Pressure Value
Fig. 3.5 To open the drain valve, turn the drain valve knob 180° counterclockwise. NOTE If the drain valve knob is turned more than 180°, any mobile phase that drains out may contain air bubbles. This is normal. Fig. 3.6 LC-20AB...
Page 46 22MPa or more has been activated. Observe the condition of the mobile phase that flows from the end of the drain tubing for about 10 seconds. The mobile phase should flow continuously and be free of air bubbles. LC-20AB...
Page 47 • If no mobile phase emerges: Attach the syringe needle to the disposable syringe Drain tubing (provided), insert the tip of the needle into the end of Syringe needle the drain tubing, and draw the mobile phase through Disposable syringe the tubing. Fig. 3.7 LC-20AB...
Page 48 3. Preparation This page is intentionally left blank. LC-20AB...
Page 49 Basic Operation Contents Setting Parameters ..................4-2 Operation ......................4-5 Plunger Rinsing When Buffer Solution is Used as Mobile Phase ....4-6 Replacing the Mobile Phase ................4-10...
Page 50: Setting Parameters
The ranges, steps, initial values and their applicable modes of parameters are listed in the following table. Parameter Range Steps Initial Value flow 0 ∼ 10.0000 mL/min 0.0001 mL/min 0 mL/min bconc 0.0 ~ 100.0% 0.1% 0.0% 4.1.1 Prior to Key Operation Press to show the operation key. LC-20AB...
Page 51: Setting Flow Rate
When an item is accessed, the cursor blinks in the 0.0MPa B:™ 0.0% display field, prompting value input. Press further to access the other auxiliary functions. [bconc] After setting a flow rate, press to return to the initial screen. LC-20AB...
Page 52: Setting The Concentration Of Mobile Phase B
∗ To cancel the new setting, press 0.0MPa B: 20.0% In the initial screen, the concentration of mobile phase A is automatically calculated and displayed. Concentration of mobile phase A [A:] (%) = 100 (%) - Concentration of mobile phase B [B:] (%) LC-20AB...
Page 53: Operation
Pump indicator Pressure display To stop operation, press again. The pumping stops, and the pump indicator goes out. CAUTION Close the drain valve if necessary. When it is left opened, mobile phase could flow out of the drain due to atmospheric pressure. LC-20AB...
Page 54: Plunger Rinsing When Buffer Solution Is Used As Mobile Phase
Draw distilled water into the disposable syringe, and fill the rinse flow line with liquid. ∗ Attach a syringe needle to the tip of the disposable syringe before use. Return the PTFE tubing connected to the automatic rinsing kit's outlet to the original state. LC-20AB...
Page 55: Operation
Pump indicator Check whether liquid is coming out of the tip of the PTFE tubing connected to the automatic rinsing kit's outlet. If liquid is coming out, press . The pump indicator goes out and the pump stops. Fig. 4.2 LC-20AB...
Page 56: Operation Method For Manual Rinsing
Right rinse- 1 to the left rinse-tubing inlet and connect the solution outlet disposable syringe (accessory). ∗ Attach a syringe needle to the tip of the disposable syringe before use. Rinse tubing Fig. 4.4 LC-20AB...
Page 57: Operation
Draw rinse solution into the disposable syringe and reconnect it to the PTFE tubing. Inject rinse solution into the rinse flow line using the disposable syringe. ∗ Manual rinsing is possible whether the pumps are running or not. LC-20AB...
Page 58: Replacing The Mobile Phase
0.0000ml A:100.0% replacement of the mobile phase is 100%. • When replacing mobile phase A, set [bconc] to 0.0MPa B: 0.0% 0.0%. • When replacing mobile phase B, set [bconc] to 100.0%. Concentration of mobile phase B [bconc] 4-10 LC-20AB...
Page 59 The mobile phase in the flow line downstream of the manual injector will be replaced. ∗ The flow rate should be set so that the pressure will be lower than the maximum column pressure. Fig. 4.10 LC-20AB 4-11...
Page 60: When New Mobile Phase Is Not Miscible With The Old Mobile Phase
"4.3 Plunger Rinsing When Buffer Solution is Used as Mobile Phase" P. 4-6 CAUTION When the instrument is to remain inactive following operation with a buffer solution, be sure to replace the buffer solution in the flow lines with distilled or de-ionized water to prevent deposition of buffer solution crystals. 4-12 LC-20AB...
Page 61 Application Operation Contents Display Panel ....................5-2 Parameter in Auxiliary Functions ..............5-12 VP Functions....................5-27 Creating Time Program.................. 5-44 Control by CBM-20A or CBM-20Alite System Controller ....... 5-54 Control by SCL-10Avp or SCL-10A System Controller ........5-55 Connection to External Input/Output Terminals ..........5-56...
Page 62: Display Panel
P r e s s f u n c o r V P VP function settings • Mobile phase monitor • Product information • Maintenance information • Validation support • Calibration support Time program edit screen P.5-44 U S E D L E F T 3 2 0 Time program editing LC-20AB...
Page 63: Basic Setting Screen And Auxiliary Function Setting Screen
0 . 0 M P a B : 0 . 0 % Concentration of P.4-4 mobile phase 0 . 0 0 0 0ml A : 1 0 0 . 0 % 0 . 0 M P a B : ™ 0 . 0 % LC-20AB...
Page 64 I n p u t 0 . 1 - 1 0 . 0 m L Purge max. pressure limit P.5-18 0 . 0 0 0 P - P M A X ™ 0 . 0 I n p u t 1 - 1 0 M P a LC-20AB...
Page 65 1 : L 2 : R 3 : M i d Zero adjustment of pressure sensor P.5-20 0 . 0 0 0 Z E R O A D J E n t e r t o Z e r o A d j LC-20AB...
Page 66 S e t P r e s s U n i t Pressure recorder full scale P.5-23 0 . 0 0 0 R A N G E ™ R A N G E 5 . 0 M P a LC-20AB...
Page 67 0 . 0 0 0 0ml A : 1 0 0 . 0 % 0 . 0 0 0 D E G A S P R S 0 . 0 M P a B : 0 . 0 % - 9 3 k P a ( O K ) LC-20AB...
Page 68: Vp Function Screen
P r e s s f u n c o r V P L 2 0 1 2 0 0 0 0 0 0 0 Software version No. P.5-31 S / W I D : V * . * * L C - 2 0 A B LC-20AB...
Page 69 E n t e r t o D i s p l a y Operation log P.5-33 O P E R A T I O N L O G E n t e r t o D i s p l a y LC-20AB...
Page 70 E n t e r t o S t a r t Leak sensor test P.5-37 L E A K S E N S O R T E S T S o a k a n d E n t e r 5-10 LC-20AB...
Page 71 C B M P A R A M E T E R P r e s s f u n c o r V P E n t e r t o S e l e c t LC-20AB 5-11...
Page 72: Parameter In Auxiliary Functions
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. 5-12 LC-20AB...
Page 73: Control Settings Group
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. LC-20AB 5-13...
Page 74: Showing The Auxiliary Function Screen
Select the desired group and press 0.000 PMAX ™0.0 Input 1 - 44 MPa Press and select the desired parameter. Press to move to the other functions. Press to show the group screen. Press to return to the initial screen. 5-14 LC-20AB...
Page 75: Parameter Settings Group
0.000 PMIN ™0.0 situations. Input 0 - 44 MPa • When the mobile phase in the reservoir bottle is depleted and air is delivered into the flow line. • When a leakage occurs in the flow line. LC-20AB 5-15...
Page 76 All channels are set to the A side. Channel 1 is set to the B side. FCV-11AL Channel 2 is set to the B side. Channel 3 is set to the B side. The above settings can be combined in use. 5-16 LC-20AB...
Page 77 0.000 BCOMP ™.45 independently. Input 0.00-3.00 The setting units are (GPa) . Enter the value using numeric keypad, and press . The initial setting is 0.45. Mobile phase Compressibility (GPa) Water 0.45 Acetonitrile 1.20 Methanol 1.25 Hexane 1.60 LC-20AB 5-17...
Page 78 Enter a maximum pressure limit using the numeric keypad and press Set range Steps 1.0 ~ 10.0 MPa 0.1 MPa 10 ~ 102 kgf/cm 1 kgf/cm 10 ~ 100 bar 1 bar 140 ~ 1400 psi 1 psi 1kgf/cm = 0.098MPa = 0.98bar = 14.2psi 5-18 LC-20AB...
Page 79 0.000 FILE CPY ™ Enter the file number and press Input No. Deleting a Time Program [FILE DEL] The contents of the time program of the currently selected file are deleted. 0.000 FILE DEL Press Enter to Delete LC-20AB 5-19...
Page 80 The plungers move to an intermediate position. Zero Adjustment of Pressure Sensor [ZERO ADJ] Clears the value of the pressure sensor to zero in atmospheric pressure. Open the drain valve. Pressure sensor is released from the pressure. 0.000 ZERO ADJ Enter to ZeroAdj Press 5-20 LC-20AB...
Page 81 Channel numbers when this instrument is 1-12 connected to system controller. Disables Key Entry [KEY CLOSE] Press to prohibit the key entry. After this, key operation is not available. 0.000 KEY CLOSE ∗ To release this function, press while pressing Enter to Close LC-20AB 5-21...
Page 82 Enter the desired value and press 0:Clear 1:Set Set value Function Cancels system protection. Set system protection. ∗ To clear [ERROR P.MAX], press . It turns the alarm off, and the flow rate will be increased as before. 5-22 LC-20AB...
Page 83 Sets the link destination of system controller. Enter the desired value and press 0.000 CBM LINK ™ 0:Int 1:Ext Set value Function Link with CBM-20Alite (option) inside of the instrument. Link with external system controller by optical cable connected to [REMOTE] connector. LC-20AB 5-23...
Page 84: Beep Mode
If [1] is set, the initial screen will be as shown on the right while the program is running. However, if other monitor 0.0000ml T 1.00 functions are set, this screen may not be displayed. 0.0MPa B: 0.0% 5-24 LC-20AB...
Page 85 Enter the set value and press 0.000 MON SV ™ Set value Function 0:Clear 1:Set Clear this function Set this function If [1] is set, the initial screen will be as shown on the right. 0.0000ml A:100.0% 0.0MPa SV LC-20AB 5-25...
Page 86 • When the pressure value is standard, [(OK)] is 0.000 DEGAS PRS displayed. - 93 kPa (OK) • When the pressure value is not standard, [(NG)] is displayed. • When the DGU-20A degasser unit is not connected, [(NOT CONNECTED)] is displayed. 5-26 LC-20AB...
Page 87: Vp Functions
∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. LC-20AB 5-27...
Page 88 ∗ Operation in the table head shows the types of operation described below. Display : Check the monitor. : Press to activate the function. Numeric keypad : Press to enter a value and press to determine the value. 5-28 LC-20AB...
Page 89 ∗ To return to the previous screen, press MOBILE PHASE A 0 / ™000 mL Follow the further instructions of the selected function. To select a different VP Function Group, press repeatedly. To select the desired function, press To return to the initial screen, press LC-20AB 5-29...
Page 90 ALARM LEVEL ™0% setting value (%). The pumping will continue. Input 1-99,0:OFF Input the value, and press Warning screen When 0% is registered, this function will be deactivated. WARN : M.PHASE-A Press CE Key 5-30 LC-20AB...
Page 91: Product Info
SERIAL NUMBER L2012000000 Showing S/W Version No. [S/W ID] Shows the name of software (same as the model name) and version. S/W ID: V*.** LC-20AB 5.3.5 Maintenance Information Group This group provides the maintenance-related information. MAINTENANCE Press func or VP Showing Total Operating Time [TOTAL OP TIME] Shows the total operating time of this instrument.
Page 92: Maintenance Log
228-30000-91 was replaced on May 12, 2003. If less than 10 logs are recorded, the screen displays the LOG 1 03-05-12 message as shown on the right. P/N:228-30000-91 Press to return to the title screen. No more Logs 5-32 LC-20AB...
Page 93: Error Log
May 12, 2003. If less than 10 logs are recorded, the screen displays the LOG 1 03-05-12 message as shown on the right. CHANGE PASSWORD Press to return to the title screen. No more Logs LC-20AB 5-33...
Page 94: Time Hh:mm:ss
When the instrument is controlled by a system controller, ™0:00:00 the time is transmitted in connecting. Example: Setting 5:30:55 p.m. Enter hour, minute and second in 2 digits. Entered The display uses the 24-hour clock. TIME HH:MM:SS 17:30:55 Press 5-34 LC-20AB...
Page 95 The date and results are recorded. (A) 1.99min left NOTE When measuring, ensure that parameters for solvent 1.000 0.09 compressibility compensation are set correctly. (A) 0.99min left "Setting Fine Adjustment of Compressibility Compensation [ACOMP] [BCOMP]" P. 5-17 PLS:A 0.09MPa OK Enter to Memory LC-20AB 5-35...
Page 96 Enter a file number 0-9 of time program in which the default gradient test program is to be copied. This file is GE TEST PROGRAM automatically loaded for execution. Copy to File ™ ∗ The test program is the gradient time program described in P.7-21. 5-36 LC-20AB...
Page 97 Support Group. SENSOR GOOD "Setting Operation Level of Leak Sensor [LEAK THR]" P. 5-39 NOTE After wetting and testing the leak sensor, wipe away the water on the leak tray completely. "8.10 Wiping of Leak Tray" P. 8-25 LC-20AB 5-37...
Page 98: Input Password
Reset when the pressure sensor is replaced. PRESS COMP FACT Enter the value as a multiple of 100 for PRS-1 (in [1:] ) 1:™456 5:8535 and PRS-5 (in [5:] )[XX.XX] shown in the data label of the pressure sensor. 5-38 LC-20AB...
Page 99 0:20A 1:VP 2:10A Set value System controller CBM-20A/20Alite SCL-10Avp SCL-10A Initializing Parameters [INITIALIZE PARAM] Initializes the parameters and deletes the time programs. Press to return to the default value and to delete the time programs. INITIALIZE PARAM Enter to Init LC-20AB 5-39...
Page 100: Change Password
To return to the title screen on the right, press NOTE When the instrument is not connected with CBM-20A/ 20Alite, or is set to local mode, the CBM parameter setting screen will not appear, even is pressed. 5-40 LC-20AB...
Page 101 Showing Serial No. [SERIAL NUMBER] Shows the serial No. of CBM which controls the instrument. SERIAL NUMBER L20220000000 Showing S/W Version No. [S/W ID] Shows the name of software and version No.of CBM which controls the instrument. S/W ID: V*.** CBM-20Alite LC-20AB 5-41...
Page 102 USE GATEWAY ™ Enter the value and press 0:No 1:Yes Set Value Default Gateway Not use Setting IP Address [IP ADDRESS] Sets IP address of CBM which controls the instrument. Enter the value and press IP ADDRESS ™92.168.1.250 5-42 LC-20AB...
Page 103: Subnet Mask
To connect to LCsolution To connect to C-R8A To connect to C-R7A/C-R5A To connect to C-R4A To connect to C-R6A (without extended ROM board) To connect to C-R6A (with extended ROM board) NOTE Value other than above are invalid. LC-20AB 5-43...
Page 104: Application Operation
1:If FCV-10ALvp is set for the [FCV TYPE] parameter, the setting range is 1, 2, 3, 4. If FCV-11AL (S) is set, the range is 0-123, using combinations of 0, 1, 2 & 3. "Selecting Flow Channel Valve Type [FCV TYPE]" P. 5-23 5-44 LC-20AB...
Page 105 310 steps remain. Press Editing screen appears. Elapsed time from program start (minutes) Command ™IME FUNC VALUE Set value 0.01 - 999.99min For the following programming procedure, refer to "5.4.3 Time Program Creating Flow" P. 5-46. LC-20AB 5-45...
Page 106 Input the Value (except for STOP) 5.00 FLOW™VALUE 0.0 - 10.0000 mL Input Stored ™5.00 FLOW4.0000 0.01 - 999.99min Make settings for the next one. ∗ The displayed screen is the one set previously. ∗ To return to the previous step, press 5-46 LC-20AB...
Page 107 STOP Fig. 5.2 Press Initial screen appears. 1.0000ml A:100.0% 0.0MPa B: 0.0% Press The number of steps already created and USED LEFT remaining steps appear. Press Time setting The setting screen appears. ™IME FUNC VALUE 0.01 - 999.99min LC-20AB 5-47...
Page 108 Step 2 Press to enter the Determination of step 2 time. Press to select [FLOW]. 20.00 FLOW5.0000 Press to enter the flow rate. 0.01 - 999.99min Step 3 Press Select of command 30.00 ™LOW 0.0 - 10.0000 mL 5-48 LC-20AB...
Page 109 3-step time program has created. USED LEFT Press to save the time program. Initial screen of step 1 appears. USED LEFT Saving ... 1.0000mlA:100.0% 0.0MPa B: 0.0% NOTE When setting several steps, they are ordered automatically in sequence. LC-20AB 5-49...
Page 110 B Conc. B (%) is shown at right. (The initial value is 0.0%.) Step TIME (min) FUNC VALUE (%) 20.00 BCNC 100.0 30.00 BCNC 100.0 30.01 BCNC 40.00 STOP "5.4.4 Creating Time Program" P. 5-47 Fig. 5.3 5-50 LC-20AB...
Page 111: Deleting Steps
There are two ways to stop the time program: • Press to stop the running program forcefully. The [prog.run] indicator goes out. • Insert [STOP] command of the program. "5.4.8 Commands Used Only For Time Program" P. 5-52 LC-20AB 5-51...
Page 112: Setting Loop Count Of Time Program [Loop]
The time program will stop when [LOOP] has finished. • Steps set after [LOOP] command will deactivate except for [GOTO] command. • Value of [LOOP] command can be set to 255. When [0] is set, time program will be repeated 256 times. 5-52 LC-20AB...
Page 113 Set the EVENT output terminal of the newly called up file specified in step 4 as [1]. 0.000 EVENT "Setting EVENT Output Terminals [EVENT]" 0,1,2 or 12 P. 5-17 Set the file number back to the original file number. "File Number [FILE NUM]" P. 5-19 LC-20AB 5-53...
Page 114: Control By Cbm-20A Or Cbm-20Alite System Controller
B, maximum pressure limit, minimum pressure limit, solenoid valves, pumping ON/OFF, time program, etc. and control binary high-pressure gradient. Also by combining the SIL-20A autosampler, auto purging function is available. Refer to the CBM-20A/20Alite instruction manual. 5-54 LC-20AB...
Page 115: Control By Scl-10Avp Or Scl-10A System Controller
[LP.GE] (i.e., set to [ISO.], [B.GE], or [T.GE]), the concentration of the mobile phase B at the instrument will not be controlled from the system controller. A mixture of the two liquids will be delivered at the concentration of mobile phase B set directly at the instrument. LC-20AB 5-55...
Page 116: Connection To External Input/Output Terminals
[0]. common terminal. ERR IN Error input terminal. To detect error. Duration of shorting (tc) Common terminal for [PUMP ON], [PUMP OFF], [START], and [ERR should be as follows. IN]. 0.5 sec < tc < 10 sec. 5-56 LC-20AB...
Page 117: Connection Of Event Cable
If [EVENT1] or [EVENT2] signal is used, set [EVENT] and [EXT-S] parameters. 0.000 EVENT "Setting EVENT Output Terminals [EVENT]" P. 5-17 0,1,2 or 12 "External Signal Functions for EVENT Output Terminals [EXT-S]" P. 5-22 0.000 EXT-S Input 0 - 3 LC-20AB 5-57...
Page 118 5. Application Operation This page is intentionally left blank. 5-58 LC-20AB...
Page 119: Troubleshooting
Troubleshooting Contents Troubleshooting and Corrective Action ............6-2 Error Message....................6-5...
Page 120: Troubleshooting And Corrective Action
For more detailed procedures, refer to the indicated page. If the problem cannot be resolved even after taking the indicated measures, or if there are problems not included in the following tables, contact your Shimadzu representative. Symptom...
Page 121 4:NOTE (Pumping at low flow rate of 50µl/min or less) Take care not to pump at high flow rate and high pressure (approx. 3ml/min (20MPa) or more) before pumping at low flow rale of 50µl/min or less. (Pumping may be unstable because of the seal wearing.) LC-20AB...
Page 122 ∗ 3:How to check clogging of filter Take off the filter part of the suction filter and record a pressure waveform. If the pressure waveform improves by taking off the filter, that suction filter is clogged. LC-20AB...
Page 123: Error Message
Cause: ROM error (electric failure) M A I N R O M F A I L U R E Action: Turn power OFF and contact your Shimadzu representative. S U B R O M F A I L U R E...
Page 124 E R R O R B - H O M E P O S Action: Turn the power OFF and ON again, and press (Home position error) . If this message still appears, turn the power OFF and contact your Shimadzu representative. Alarm Cause: Pump discharge pressure has exceeded O P E N D R A I N V A L V E [P-PMAX] during purging.
Page 125 [NG], turn the power OFF once and turn it ON again. If the vacuum pressure is [OK], it is of no matter, for the degasser is returning to run automatically. "Monitoring Vacuum Pressure in Degasser Unit [DEGAS PRS]" P. 5-26 LC-20AB...
Page 126 6. Troubleshooting This page is intentionally left blank. LC-20AB...
Page 127: Chapter 7 Hardware Validation
Hardware Validation This chapter provides instruction on hardware validation, which verifies the performance of individual components and the instrument as a whole. Contents Overview of Hardware Validation..............7-2 Implementation of Hardware Validation ............7-3 Validation Precautions ..................7-4 Equipment Required for Validation ..............7-5 Validation: Pump ....................
Page 128: Overview Of Hardware Validation
The operational protocol and criteria for this component and the HPLC system are described in this chapter to assist the user in conducting validation. Refer to each the instruction manual for each component for operational protocol of that specific component. LC-20AB...
Page 129: Implementation Of Hardware Validation
After any maintenace, component performance must be re-validated. The type of validation depends on the actual work done. If the maintenance inspection cannot be performed solely by the specific component validation, system validation is required. NOTE Maintenance information and results of hardware validation must be recorded and kept for future reference. LC-20AB...
Page 130: Validation Precautions
• Provide adequate installation surface and space The weight of LC-20AB is 13kg. During installation, consider the entire weight combined with other LC components. The lab table on which this instrument is installed should be strong enough to support the total weight of the LC system.
Page 131: Equipment Required For Validation
For inspection of the flow rate accuracy for the solvent delivery module. Measuring flask Obtain a 5mL-measuring flask. For inspection of the injection volume accuracy for the autosampler. Electronic balance The balance must be calibrated and able to perform measurement with a 0.001g precision at the time of inspection. LC-20AB...
Page 132: Standard Reagents For Validation
For inspection of the wavelength accuracy for the spectrofluorometric PTFE block assembly 228-34319-91 detector. Column Particle size: 5µm Shim-pack VP-ODS Column Dimension: I.D. 4.6mm × length 150mm 228-34937-91 (An equivalent ODS column may also be used.) LUNA C18(2) For the system validation. 00F-4252-E0 LC-20AB...
Page 133: Validation: Pump
[VALIDATION]. Press three times to display [MEMORY CHECK]. MEMORY CHECK ROM OK / RAM OK Press "Checking Memory [MEMORY CHECK]" P. 5-35 CHECK CRITERIA : [ROM OK / RAM OK] is displayed on the screen. LC-20AB...
Page 134: Firmware Version Check
Press two times to display the version number. S/W ID: V*.** "Showing S/W Version No. [S/W ID]" P. 5-31 LC-20AB CHECK CRITERIA : Version number appears. The number is same as the administrated one. 7.5.4 Display, LED Test Objective To check the operation of display and LEDs.
Page 135: Flow Rate's Control Parameter
Make a note of the displayed two values (1: [PRS- PRESS COMP FACT 1] and 5: [PRS-5]) of [PRESS COMP FACT]. 1:1456 5:8535 "Setting Pressure Sensor Sensitivity Compensation Factor [PRESS COMP FACT]" P. 5-38 CHECK CRITERIA : Parameters appear. LC-20AB...
Page 136: Pumping Stability Test
Input 0.00-3.00 Press once. Make a note of the current value of [BCOMP] 1.000 BCOMP 0.45 (compressibility compensation) and set it to [0.45] Input 0.00-3.00 in the way described above. Press two times to return to the initial screen. 7-10 LC-20AB...
Page 137 PULSE CHECK B CRITERIA:0.20MPa Repeat steps 10 to 12 to measure the pressure fluctuation width for pump B. CHECK CRITERIA: The measurement results for the pressure fluctuation width for pump A and pump B do not exceed 0.20MPa. LC-20AB 7-11...
Page 138: Resetting After Check
Turn the power switch ON. Initial screen appears. 2.0000ml A:100.0% 0.0MPa B: 0.0% Press to activate setting of the flow rate. Cursor (positioned to prompt input) Press to set the flow rate to [1mL/min]. ™.0000ml A:100.0% 0.0MPa B: 0.0% 7-12 LC-20AB...
Page 139 While pumping, change [P.MAX] and [P.MIN] as follows: [P.MAX] = actual pressure + 1.0MPa. 1.0000ml A:100.0% [P.MIN] = actual pressure - 1.0MPa. 10.0MPa B: 0.0% ∗ For example, when the actual pressure value is [10.0MPa], set [11.0MPa] as [P.MAX] and [9.0MPa] as [P.MIN]. LC-20AB 7-13...
Page 140 The actual pressure should fall below the [P.MIN] value set by step 12, and in several seconds, 5.0MPa B: 0.0% [ERROR P-MIN] should appear. Press to clear [ERROR P-MIN]. ERROR P-MIN 5.0MPa B: 0.0% CHECK CRITERIA : [ERROR P-MAX] and [ERROR P-MIN] appear. 7-14 LC-20AB...
Page 141: Leak Sensor Test
LEAK SENSOR TEST SENSOR GOOD CHECK CRITERIA : [SENSOR GOOD] appears on the screen. NOTE After wetting and testing the leak sensor, wipe away the water on the leak tray completely. "8.10 Wiping of Leak Tray" P. 8-25 LC-20AB 7-15...
Page 142: Flow Rate Accuracy Test
[0.45] as [ACOMP]. 1.000 ACOMP 0.45 Input 0.00-3.00 Press once. Make a note of the current value of [BCOMP] 1.000 BCOMP 0.45 (compressibility compensation) and set it to [0.45] Input 0.00-3.006 in the way described above. 7-16 LC-20AB...
Page 143 STOP 3) Stop the stopwatch when the water is pooled Measuring 5mL. cylinder START NOTE Fig. 7.3 Make sure that the end of the tubing remains above the water in the measuring cylinder throughout the measurement. LC-20AB 7-17...
Page 144: Resetting After Check
CHECK CRITERIA : The flow rate accuracy for both pump A and pump B is within ± 2.00%. Resetting after Check Press repeatedly until [PARAMETER] appears. Then, press Press repeatedly until [ACOMP] or [BCOMP] appears. Return the value to the recorded value by step 4, 7-18 LC-20AB...
Page 145: Validation: Gradient Pump System
Data processor Chromatopac, LC workstation or similar Resistor tube Distilled water (How to prepare) <When using Shimadzu's standard reagents for validation> Mobile Weigh 20mg of caffeine (250mg/L, Part No. 228-45725-06) and bring it to ∗ phase 500mL volume with water.
Page 146 • If a system controller or LC workstation is used, select time program from each menu, and create the program. • If control is local, create the time program on pump. Time (min.) Fig. 7.4 7-20 LC-20AB...
Page 147 For C-R5A LINE PROGRAM LPRINT LEVEL; WAIT 30 GOTO 10 Zero the baseline, start plotting on the data processor and continue with the gradient time program. If the Chromatopac is used as data processor, run the BASIC program. LC-20AB 7-21...
Page 148 (Absorbance level at set concentration of 90%) concentration of 0%) Actual concentration of 90% = × 100 (Absorbance level at set (Absorbance level at set concentration of 100%) concentration of 0%) CHECK CRITERIA : Within ± 1.0% of set value 7-22 LC-20AB...
Page 149: System Validation
Weigh 20mg of anhydrous caffeine, transfer to a 100mL volumetric flask and dilute to volume with Sample water. Transfer 1mL of the solution to a 10mL volumetric flask, and dilute to volume with water. Water HPLC grade, or equivalent 2-propanol HPLC grade, or equivalent LC-20AB 7-23...
Page 150: Checking And Preparing The Lc System
(methanol, acetonitrile, etc.) > Clean the flow lines with water. Pass water through the flow lines for 10 minutes, at a rate of 2mL/min. 7-24 LC-20AB...
Page 151: Validation Procedure
"Parameter Settings for Isocratic System Validation" P. 7-26 See the autosampler’s instruction manual for setting procedures. Set the data processor parameters. "Parameter Settings for Isocratic System Validation" P. 7-26 See the data processor's instruction manual for setting procedures. LC-20AB 7-25...
Page 152: Parameter Settings For Isocratic System Validation
: 1000 ATTEN : 10 (1,024mAUFS) SLOPE : 1000 MIN.AREA : 100000 STOP.TM CHECK CRITERIA The RSD (C.V.)'s obtained must satisfy the following criteria: Retention time RSD must not exceed 0.5%. Peak area RSD must not exceed 1.0%. 7-26 LC-20AB...
Page 153: Validation Of Gradient Lc System
(b) the column outlet and the detector inlet, has an I.D. of less than 0.3mm, and is shorter than 300mm. Keep the liquid volume that is not in the column as low as possible. Fig. 7.8 LC-20AB 7-27...
Page 154: Validation Procedure
7.9"). Validation Procedure Pump’s display screen Set the pumping flow rate to 1mL/min, and set the concentration of mobile phase B parameter to 40%. 1.0000ml A: 60.0% See the pump's instruction manual for setting 0.0MPa B: 40.0% procedures. 7-28 LC-20AB...
Page 155 Validation" P. 7-30 See the data processor’s instruction manual for setting procedures. Monitor the baseline. When the baseline has stabilized, press the detector key. Then inject 10µL of mobile phase and verify that no peaks are observed the second time. LC-20AB 7-29...
Page 156: Parameter Settings For Gradient System Validation
: 1000 ATTEN : 10 (1,024mAUFS) SLOPE : 1000 MIN.AREA : 100000 STOP.TM CHECK CRITERIA The RSD (C.V.)'s obtained must satisfy the following criteria: Retention time RSD must not exceed 0.5%. Peak area RSD must not exceed 1.0%. 7-30 LC-20AB...
Page 157: If Validation Fails
For troubleshooting procedures for individual system components, see the applicable instruction manuals. • If a cause cannot be determined, contact your Shimadzu representative: If you are unable to determine the cause of the failure, or if you are unclear about troubleshooting or corrective action procedures, contact your Shimadzu representative.
Page 158 7. Hardware Validation This page is intentionally left blank. 7-32 LC-20AB...
Page 159 Maintenance Contents Periodic Inspection and Maintenance .............. 8-2 Replacement of Plunger Seal ................8-5 Cleaning and Inspection (Replacement) of Plungers and Diaphragms ..8-10 Cleaning of Check Valves ................8-15 Inspection (Replacement) and Ultrasonic Bath Cleaning of Check Valves..8-16 Inspection (Replacement) and Ultrasonic Bath Cleaning of Line Filter..
Page 160: Periodic Inspection And Maintenance
Periodic Inspection and Maintenance It is necessary to perform periodic inspections of this instrument to ensure its safe use. It is possible to have these periodic inspections performed by Shimadzu service representatives on a contractual basis. For information regarding the maintenance inspection contract, contact your Shimadzu representative.
Page 161: List Of Periodic Inspection And Maintenance
• Particulates in mobile phase clog the filter × P.8-21 cleaning of suction filter in prolonged use. • Sealing efficiency decreases when the Replacement of Drain valve × P.8-22 drain valves are worn. Pump ASSY lubrication × • Contact your Shimadzu representative. Fuse replacement × P.8-24 LC-20AB...
Page 162: Check After Inspection And Maintenance
To filter particulates from the mobile phase phase to eliminate. in order to protect the column. Fig. 8.1 8.1.3 Check after Inspection and Maintenance After inspection and maintenance, check any leakage during pumping. "6.1 Troubleshooting and Corrective Action" P. 6-2 LC-20AB...
Page 163: Replacement Of Plunger Seal
The pump indicator illuminates for a short while, then goes out. The right plungers for both pumps A and B are retracted as far as possible. "Plunger Set [P-SET]" P. 5-20 Pump indicator illuminates. Press two times. Initial screen appears. LC-20AB...
Page 164: Removing Pump Head
SUS tubing Outlet check valve Inlet check valve Male nut PEEK Fig. 8.3 Using the provided Allen wrench, alternately loosen the two hex socket head bolts in the pump head and remove. Hex socket head bolt Allen wrench Fig. 8.4 LC-20AB...
Page 165: Replacing Plunger Seal
After removing the seal, wipe the plunger head inner Plunger seal hole side and plunger seal outlet with clean gauze soaked in 2-propanol. If seal material adheres to these surfaces, the sealing efficiency of the plunger cannot be maintained. Fig. 8.7 LC-20AB...
Page 166: Installing Pump Head
Allen wrench. socket NOTE head bolts Screw the bolts in alternately 90°. At the end, tighten the bolts securely with the Allen wrench. Allen wrench Fig. 8.10 LC-20AB...
Page 167: Check After Replacement
When the phenomena above happen even after replacement of the plunger seals, the surfaces of the plunger may be scratched or nicked, in which case the plunger must be replaced. "8.3 Cleaning and Inspection (Replacement) of Plungers and Diaphragms" P. 8-10 LC-20AB...
Page 168: Cleaning And Inspection (Replacement) Of Plungers And Diaphragms
Remove the head holder. Plunger Hex socket head bolt Fig. 8.13 CAUTION Ease the head holder out gently, keeping it aligned with the plunger at all times. If it is pulled out forcefully, the plunger could be bent. 8-10 LC-20AB...
Page 169: When The Head Holder Is Difficult To Remove
When inserting the box driver into the plunger holder, be careful not to bend the plunger. Handle Rubber rings Fig. 8.15 Wipe any seal material with clean gauze soaked in 2-propanol. Seal material will interfere the sealing efficiency. Plunger holder Assy Clean/ Inspect Plunger Fig. 8.16 LC-20AB 8-11...
Page 170: Inspection (Replacement) Of Diaphragms
Fig. 8.18 8.3.4 Re-Installing after Inspection Mount the diaphragm to the body. Body Diaphragm NOTE Be sure to mount the diaphragm with the correct orientation, as shown on the "Fig. 8.19". Plunger holder Assy Fig. 8.19 8-12 LC-20AB...
Page 171 Allen wrench. NOTE Tighten the hex socket head bolts alternately, in 90º increments. At the end, tighten the bolts securely with the Allen wrench, holding the long part of the wrench like a handle. LC-20AB 8-13...
Page 172: Check After Cleaning And Inspection (Replacement)
If Air Is Leaked Check the connection between the rinse tubing and the head holder and, if there are no irregularities, disassemble the components and check that the diaphragm is mounted properly. 8-14 LC-20AB...
Page 173: Cleaning Of Check Valves
"4.1.2 Setting Flow Rate" P. 4-3 "4.1.3 Setting the Concentration of Mobile Phase B" P. 4-4 Pump indicator illuminates Press Pumping of 2-propanol will begin at a rate of 2mL/ min, and the pump indicator will illuminate. Pump for at least 1 hour. LC-20AB 8-15...
Page 174 • Empty the reservoir and tubing of all mobile phase, and unscrew and remove the filter bushing from the pump inlet. Hold the outlet check valve using the provided wrench SUS tubing Loosen the male nuts at both ends of SUS tubing using the other wrench and remove SUS tubing. Hold Fig. 8.25 8-16 LC-20AB...
Page 175: Inspection (Replacement) And Ultrasonic Bath Cleaning Of Check Valves
5 minutes. Screw the inlet and outlet check valves back into the pump head, and tighten with the wrench. Return SUS tubing, the inlet-side tubing, the suction filters, and the reservoir bottle to their original states. LC-20AB 8-17...
Page 176 The pump indicator illuminates, and liquid flows from the drain tubing. If liquid flows continuously, the check valves are functioning normally. If the liquid does not flow continuously from the drain tubing, the check valves must be replaced. 8-18 LC-20AB...
Page 177: Inspection (Replacement) And Ultrasonic Bath Cleaning Of Line Filter
Screw the line filter back into the line filter Line filter opening, and tighten it with the open-ended wrench. Screw the male nut on the SUS tubing back into Male nut the line filter, and tighten the nut with the open- ended wrench. Fig. 8.31 LC-20AB 8-19...
Page 178: Replacing Frit
Remove the frit on the end of the line filter body using nippers. Nippers Frit Fig. 8.32 Put a new frit into the line filter body. New frit Reinstall the line filter. Perform steps 6 through 7 of the procedure in P.8-19. Filter body Fig. 8.33 8-20 LC-20AB...
Page 179: Inspection (Replacement) And Ultrasonic Bath Cleaning Of Suction Filter
Turn the power switch ON. The initial screen appears on the display. Pump water at 1mL/min for 10 minutes. Check that air bubbles do not accumulate inside the solvent tubing. If they do, the tubing must be replaced. LC-20AB 8-21...
Page 180: Replacement Of Drain Valve Assy
If the drain valve assy is inserted slantwise and forcefully, the seal can deform. As a result, the sealing of the seal cannot be maintained. Seal Fig. 8.36 Screw the new drain valve ASSY as far as it will go to close. Fig. 8.37 8-22 LC-20AB...
Page 181 8.8 Replacement of Drain Valve ASSY Screw the knob to the drain valve ASSY and insert the cap into the knob, while the kob position is vertical. Screw Knob Fig. 8.38 LC-20AB 8-23...
Page 182: Replacement Of Fuse
Coin Unscrew by using a coin Fig. 8.39 Place new fuses into the fuse holder. Fuse holder New fuse Coin Screw by using a coin Fig. 8.40 Push the fuse holder in and fix by using a coin. 8-24 LC-20AB...
Page 183: Wiping Of Leak Tray
• Wipe the dirt on the leak tray with cloth soaked in water. Leak sensor Re-rotate the protective plate. Leak tray Fig. 8.42 CAUTION • Make sure to re-rotate the protective plate after the wiping. Leak sensor may not detect leakage without the protective plate. LC-20AB 8-25...
Page 184: Exterior Cleaning
Use a dry cloth to remove all moisture from the exterior surface of the instrument. NOTE Do not allow spilled water to remain on the instrument surface, and do not use alcohol or thinner-type solvents to clean the surfaces. These can cause rusting and discoloration. 8-26 LC-20AB...
Page 185 Technical Information Contents Installation ......................9-2 Specifications ....................9-37 Maintenance Parts ..................9-39 Introduction to HPLC System................. 9-41 Mobile Phase Characteristics................. 9-44...
Page 186: Technical Information
• air currents from heating or air conditioning equipment are not directed on the instrument. • sunlight does not shine directly on the instrument. • there is no vibration. • humidity stays within 20 - 85%. • place without condensation LC-20AB...
Page 187 This allows for sufficient air circulation to provide cooling and prevent the instrument from overheating and impairing the performance. Typical system configurations and required installation spaces are shown in the figures below. System 1 (with Manual Injector) System 2 (with Autosampler) (Unit : mm) Fig. 9.1 LC-20AB...
Page 188 Safety available. bracket Fasten the instrument firmly in place by attaching stacking brackets to both the right and left sides. For more details, contact your Shimadzu representative. Safety bracket An example of stacking bracket placement is shown in "Fig. 9.3".
Page 189: Power Supply Connection
Insert the connector side of the power cord into the power cord connector at the back of the instrument. Connector side Insert the plug side of the power cord into the power supply outlet. Plug side Connect to Power outlet Fig. 9.5 LC-20AB...
Page 190 Cutting SUS Tubings Position the provided file (for cutting SUS tubing) diagonally against the tubing, and cut up around SUS tubing the tubing. NOTE Cut up the tubing so that the cut surface is at a right angle. Fig. 9.6 LC-20AB...
Page 191 • Make sure that the inner diameter of the tubing is not deformed. Otherwise, the tubing may be clogged. Cutting Resin Tubings Cut off the resin tubing at a right angle using a cutter. Resin tubing Cut at right angle Fig. 9.8 LC-20AB...
Page 192 If the nut is to be connected to a union or other part that is not secured, use a second wrench to secure the union. • For a resin male nut: Tighten and loosen the nut by hand. Coupling Fig. 9.11 LC-20AB...
Page 193 Keep the plugs, and replace them when the instrument will be left not in use for a long time. NOTE • For stop plugs: Use the wrench provided to unscrew and screw in the plugs. • For resin plugs: Remove and replace the plugs by hand. LC-20AB...
Page 194 Suction filter plumbing P.9-12 Reservoir preparation P.9-11 Drain tubing plumbing Connecting leakage drain tubing P.9-13 P.9-14 Waste container preparation P.9-11 Waste container preparation P.9-11 Front cover removal P.9-11 Front cover installation P.9-18 Fig. 9.13 9-10 LC-20AB...
Page 195: Front Cover Removal
Press on the cover at the position indicated in the figure, and release to open the front cover. Fig. 9.15 Hold the front cover upward and remove. NOTE The front cover can be removed at 120 degree of open angle. Fig. 9.16 LC-20AB 9-11...
Page 196 Bottle cap cap, place the lid into the bottle mouth, and screw the bottle cap on. NOTE The filter should touch the bottom of the bottle. Replace the ferrule, bushing and coil spring on the Reservoir tubing. Fig. 9.18 9-12 LC-20AB...
Page 197 NOTE Keep the shipping cap after removal and attach it again if the instrument will not be used for a long time to prevent Shipping cap foreign matter from entering the port. Fig. 9.21 LC-20AB 9-13...
Page 198 • For connecting, cut a silicone tubing into the length in which both of the cut parts will not sag. • Set the silicone tubing so that its edge does not touch Waste container the liquid surface in the waste container. Fig. 9.23 9-14 LC-20AB...
Page 199 L-Joint Insert the other end of the silicone tubing into the waste container. Fig. 9.26 NOTE To ensure a smooth flow of liquid, insert the silicone tubing into the container with its tip pointing downward. LC-20AB 9-15...
Page 200 Drain outlet Upper unit Pour some water onto a spot near the drain outlet of the top unit, and verify that the water flows to Drain adapter the waste container. Leakage hole Lower unit Fig. 9.28 9-16 LC-20AB...
Page 201 • Set the silicone tubing so that its edge does not touch the liquid surface in the waste container. When the tip of tubing touches into the liquid of waste container, the drainage becomes difficult to flow. LC-20AB 9-17...
Page 202: Front Cover Installation
Has liquid-contacting parts made of non-metallic materials. Maximum 228-32650-91 Type 9725 use temperature: 60°C Same as type 9725, but with a position sensing switch. Non-metallic manual injector Can send signals synchronized with injection of samples to system 228-32650-93 Type 9725i controller or Chromatopac. 9-18 LC-20AB...
Page 203 Fig. 9.33 Insert the vial holder provided with the installation plate into the vial holder opening in the plate. Insert the vial provided with the installation plate Vial holder into the vial holder. Vial Vial holder hole Fig. 9.34 LC-20AB 9-19...
Page 204 Install a male nut (with long bushing) and ferrule to Waste liquid one end of each of the two waste liquid tubing tubing sections. Then attach the tubing and ferrules into ports 5 and 6 of the manual injector. Tighten the nuts. Waste liquid tubing Fig. 9.36 9-20 LC-20AB...
Page 205 (provided as manual injector Pump standard accessories). SUS tubing Insert the ends of the SUS tubing into the pump outlet and port 2 of the manual injector, and tighten the male nuts. Connect to port 2 Fig. 9.39 LC-20AB 9-21...
Page 206 Column inlet Fig. 9.40 Insert the ends of the SUS tubing into port 3 of the manual injector and the column inlet, and tighten From pump outlet the male nuts. Column inlet SUS tubing Fig. 9.41 9-22 LC-20AB...
Page 207 Arrows indicate the direction of flow Male nut PEEK Cell outlet Detector Cell inlet Male nut PEEK Blue cover Stop plug Male nut PEEK Column outlet Waste container Unscrew and remove stop plug. from Autosampler / Manual injector Fig. 9.42 LC-20AB 9-23...
Page 208 Attach caps to ports which are not used, to prevent dirt or dust entering through them. 0.5mL Plumbing 1.7mL Plumbing 2.6mL Plumbing Attach caps Attach cap to Autosampler / Manual injector to Autosampler / Manual injector to Autosampler / Manual injector Attach cap Fig. 9.44 9-24 LC-20AB...
Page 209 For installing of the mixer on a column oven, see the column oven instruction manual. Unscrew the cover installation screws attached on the right side of this instrument. Mixer Install the mixer on this instrument using the unscrewed screws. Fig. 9.47 LC-20AB 9-25...
Page 210 SUS tubing. Attach the ends of one tubing section and ferrule Pump outlet into the pump outlet and premixer inlet B, and SUS tubing Inlet B screw in the male nuts. Fig. 9.50 9-26 LC-20AB...
Page 211 NOTE If an autosampler is used, see its instruction manual for procedures. to Port 2 from Mixer outlet Fig. 9.51 Mixer Cover Installation Secure the mixer cover with the screws. Mixer cover Fig. 9.52 LC-20AB 9-27...
Page 212 Use the connectors above needed for the system. Connection instructions are provided on the following pages. [PUMP PRESS] connector [DGU PRESS] connector External input/output terminals P.9-33 P.9-33 P.5-56 [DGU/SOL.V] connector [REMOTE] connector P.9-29 P.9-32 Fig. 9.53 9-28 LC-20AB...
Page 213 • Do not bend the cable where it joins the plug. Failure to follow these above precautions could result in damage to the plug or a broken wire in the cable. Fig. 9.56 LC-20AB 9-29...
Page 214 : Check the value is [0] (initial value). (Remote mode) • [CBM LINK]: Check the value is [1] (initial value). (External connection) • [ADRS] : Enter the system controller’s connector channel number. 0.000 CBM LINK 1 0:Int 1:Ext 0.000 ADRS Input 1 - 12,85 9-30 LC-20AB...
Page 215 Refer to the CBM-20Alite instruction manual. WARNING • Only Shimadzu service representative is responsible for installation of CBM-20Alite. There is a risk of electric shock or short. • By the installation of the instrument, remove the power plug of the instrument from the power outlet.
Page 216: Connection Of [Dgu/Sol.v] Connector
0:10AL 1:11AL "5.2 Parameter in Auxiliary Functions" P. 5- • [FCV TYPE] : Enter [1]. • [SV] : Set the parameter depending on a kind of selected mobile phase. 0.000 SV ™ Select Po rt No. 9-32 LC-20AB...
Page 217: Connection Of [Pump Press] Connector
"Setting Pressure Recorder Range 0.000 RANGE ™0 [RANGE]" P. 5-23 RANGE*5.0MPa/FS Connection of [DGU PRESS] Connector [DGU PRESS] connector is to enter the pressure signal of vacuum pump in degasser unit. Refer to the instruction manual of degasser unit. LC-20AB 9-33...
Page 218: Installation Of Automatic Rinsing Kit (Optional)
Incorrect plumbing may shorten the life expectancy of the diaphragms attached to the main unit. The arrows indicate the flow of the rinse solution. Left rinse-solution outlet Right rinse-solution outlet Right rinse-solution inlet Left rinse-solution inlet Transparent tubing (Outlet) Outlet Transparent tubing (Inlet) Inlet Fig. 9.60 9-34 LC-20AB...
Page 219 Rinse tubing ASSY the way shown on the right. Fig. 9.62 The arrows indicate the flow of the rinse solution. Fig. 9.63 LC-20AB 9-35...
Page 220 Attach the automatic rinsing kit to the conical flask. Conical flask Rinsing kit base Fig. 9.64 NOTE Do not pull on the rinse tubing connected to the pump heads or any of the automatic rinsing kit tubing, or you could disconnect the tubing. 9-36 LC-20AB...
Page 221: Specifications
9.2 Specifications Specifications 9.2.1 LC-20AB Pump Unit Item Specification Pump Type Micro-volume double plunger pump (approx. 10µL/stroke) Pumping Methods Constant flow delivery 0.0001-5mL/min (1.0-40MPa) Flow Rate Setting Range 5.0001-10mL/min (1.0-20MPa) ± 1% or ± 0.5mL/min, whichever is the greater. (0.01-2mL/min) ±...
Page 222: High-Pressure Gradient System
± 0.5% Concentration accuracy (for binary gradient with water/caffeine solution, at 0.05-2mL/min, 1.0- 40MPa) Flow rates possible 0.0001-10mL/min High-Pressure Gradient System controlled by Pump Unit (LC-20AB) Item Specification Number of solvents mixed Gradient profile Step and linear possible at multiple levels Maximum program steps Max.
Page 223: Maintenance Parts
For plumbing between the inlet block and the Male nut 1.6MN PEEK 228-35403 check valve Inlet block 228-18648 Pump inlet Pressure sensor ASSY, 20AB 228-45552-91 Drain valve ASSY, B/D 228-45574-91 Right panel ASSY, 20AB 228-46513-93 Front cover Thrust 228-34469 LC-20AB 9-39...
Page 224: Electrical Parts
Electrical Parts Part Part No. Remark Fuse 5AT, 250V 072-02004-23 Switch ASSY 228-42287-91 Power supply LEA100F-24 074-80427-61 PCB LC-20AB ASSY 228-42118-92 without EEPROM PCB sensor ASSY 228-35842-92 Left panel 20AB ASSY 228-43200-93 Display panel and keypad assembly Leak sensor ASSY 228-39247-92...
Page 225: Introduction To Hplc System
9.4 Introduction to HPLC System Introduction to HPLC System The Prominence LC (LC-20A) series components are for use with Shimadzu high performance liquid chromatography (HPLC) systems, which are designed to provide high accuracy and high sensitivity analyses. Example system configurations are provided below, along with descriptions of the operations of the various components.
Page 226: Example Of Autosampler System (1)
In the column, the components are separated by means of the mutual interactions of the mobile phase and the column packing (stationary phase). The detector detects the components separated in the column, and sends the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. 9-42 LC-20AB...
Page 227: Example Of Autosampler System (2)
The detector detects the components eluted from the column, and send the signal data to a Chromatopac or PC. Mobile phase from the detector drains into the waste container. The CBM-20A system controller can control a maximum of 8 LC components (12 LC components as an option) including a maximum of 4 pump units. LC-20AB 9-43...
Page 228: Mobile Phase Characteristics
26 Ethyl iodide 1.510 0.57 27 n-Octanol 1.427 10.3 28 Fluorobenzene 1.46 0.55 29 Benzylether 1.538 30 Methylene chloride(**) 1.421 0.41 0.42 0.17 31 Anisole 1.514 32 i-Pentanol 1.405 0.61 14.7 33 1,2-Dichloroethane 1.442 0.78 0.44 0.16 10.4 9-44 LC-20AB...
Page 229 0.80 36.7 64 Dimethylsulfoxide 1.477 2.00 0.62 miscible 65 N-methyl-2-pyrolidone 1.468 1.67 Hexamethyl phosphoric 1.457 0.65 acid triamide 67 Methanol(*) 1.326 0.54 miscible 32.7 68 Nitromethane 1.380 0.61 69 m-Cresol 1.540 11.8 10.0 70 N-methylformamide 1.447 1.65 miscible LC-20AB 9-45...
Page 230 (7) Water solubility (%W) at 20°C of solvent used in liquid-to -solid adsorption. (8) Value at 20°C. (9) Function consisting of P’ (proportional to solvent strength) plus the dielectric constant, in ion chromatography. Source:A.Krstulovic and P.R.Blown, Reversed-Phase High-Performance Liquid Chromatography, Wiley Interscience, 1982. 9-46 LC-20AB...
Page 231 DGU/SOL.V Connector ......2-6 9-32 INITIALIZE PARAM ........5-39 Display Panel ...........2-2 Input PASSWORD ........5-38 Drain Tubing ..........9-13 Installation ............9-2 Drain Valve ............. 2-3 Installation Space ..........9-3 Drain Valve Knob ..........2-2 KEY CLOSE ..........5-21 LC-20AB Index...
Page 232 Plunger Rinsing ..........4-6 TIME .............. 5-34 PMAX ............5-15 Time Program ..........5-44 Power Cord Connector ........2-6 TOTAL OP TIME ........... 5-31 Power Supply Connection ....... 9-5 Troubleshooting and Corrective Action ... 6-2 Power Switch ..........2-2 LC-20AB Index...
Page 233 Index Validation Support Group ..5-10 5-28 5-34 Validation: Pump ..........7-7 VP Functions .........5-8 5-27 Wiring ............9-28 ZERO ADJ ............ 5-20 LC-20AB Index...
Page 234 Index This page is intentionally left blank. LC-20AB Index...

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